Phenotyping common bean populations and breeding lines for drought and BSM tolerance in Ethiopia

Asrat AA, Fistum A, Daniel A, Yayis R

SARI- Ethiopia

Act. 3: Deploy insect and disease resistance genes

Milestone 5: QTL for bean stem

maggot resistance

• Two populations evaluated for BSM

and drought combined stress in Ethiopia

at three locations as part of M.Sc.

Thesis

• 14 released varieties evaluated for

BSM and drought combined stress

tolerance in Ethiopia at three locations

• To estimate the phenotypic and genotypic

variability for Morpho-physiological

traits and asses the genetic variability for

drought and BSM resistance among

common bean recombinant lines

• To evaluate the performance of the

recombinant lines of two populations

from crosses G2333 x G19839 and

BAT881 x G21212 for their drought and

BSM combined resistance.

• To evaluate the contribution of

adventitious roots for BSM resistance

Objectives:

Methodology • The experiments were conducted at three locations

• Areka • Humbo • Wondogenet

• Where BSM infestation is very common • Four managed environments were created

– 1. Non stress • Chemical treated for BSM and fully irrigated

– 2.Intermittent Drought Stressed (Humbo and Wondogenet) • Stressed before flower initiation and at pod initiation

– 3. Terminal drought stress at flower initiation (Humbo and Wondogenet)

– 4. Terminal drought stress at pod initiation (Areka, Humbo and Wondogenet)

Act. 3: Deploy insect and disease resistance genes

Methodology

The materials evaluated in three groups 14 regionally and nationally released common bean varieties

82 RILs from cross G2333 x G19839 ( CIAT)

100 RILS from cross BAT881 x G21212 ( CIAT)

using

14*14 simple lattice design

Two rows plot leaving one row between plots

60 cm spacing between rows and 10 cm between

plants spacing

Act. 3: Deploy insect and disease resistance genes

• All phenology data were collected • BSM count per plant • % of lodged plants due to BSM • BSM damage score per plot • BSM damage score per Individual plant • SPAD reading • Canopy temp • Root pulling resistance • Stem Diameter • PHI • Yield • Pod wall thickness

Act. 3: Deploy insect and disease resistance genes

Data collected

Humbo non-stress

Areka stress

Areka non-stress

Humbo stress

G2333 x G19839 RIL

with adventitious root

BAT881 x G21212 RIL

Field performance of two RIL populations

under drought and BSM stress

BAT881 BAT881 x G21212 RIL

G19839

G21212 G2333

Regional varieties

G19839 x G2333 RIL

Grain yield kgha-1 NS

Gra

in y

ield

kg

ha

-1 S

tress

Performance of RILs, parents and regional varieties for combined effect of DS & BSM

at Areka, Ethiopia 2011

Grain yield kgha-1 NS

Gra

in y

ield

kg

ha

-1 S

tress

Performance of RILs, parents and regional varieties for combined effect of DS & BSM

at Humbo, Ethiopia 2011

Act. 4: Deploy drought tolerance traits in Andean gene pool

Milestone 6: 36 Andean genotypes characterized for drought traits

• 36 Andean genotypes were evaluate at Awassa Ethiopia under irrigated and stressed conditions and data on canopy temperature, root pulling resistance, PHI and grain yield

Milestone 7: Three visual scales for drought

• 64 lines developed from non-wilting parents

were evaluated at Awassa Ethiopia for wilting

resistance, root pulling resistance and PHI

Act. 4: Deploy drought tolerance traits in Andean gene pool

• Population development and schematic diagram of MARS

• Population developed from single and multiple crosses

• Cross 16357 (CAL 143 x SAB 620) x SAB 626 • Cross 16358 (SAB 628 x CAL 143) x SAB 659 • Cross 16359 (SAB 628 x CAL 143) x SAB 686

• Advanced to F3-6 generation

Act. 4: Deploy drought tolerance traits in Andean gene pool

Milestone 11: 1 population (drought-tolerant Durango x Andean) of 190

lines phenotyped for MARS (years 2 and 3), in Colombia and

Ethiopia

– 126 DAB lines evaluated in Ethiopia at two sites under drought

stress and non-stress environments using 9 x 14 alpha lattice

design

– 72 SAB lines evaluated in Ethiopia at two sites under drought

stress and non-stress environments using 9 x 8 alpha lattice

Marker -assisted recurrent selection scheme on beans

Genotyping of parents (CIAT)

Pheotyping of the whole population(Colombia

and Ethiopia)

Single and multiple cross

Cross 16357 (CAL 143 x SAB 620) x SAB 626

Cross 16358 (SAB 628 x CAL 143) x SAB 659

Cross 16359 (SAB 628 x CAL 143) x SAB 686

DAB lines

F1

F2

F3-6

Genotyping of the whole population

(CIAT)

QTL

Cycle one

Cycle two Selection of the best lines based on QTL results

Intercross the best lines (at least 10 genotypes/family )

F2

Pheotyping of the population

(Colombia and Ethiopia)

Genotyping of the population

(CIAT)

QTL

Development of the best lines

Genotyping of the selected genotypes

Description of phenoytping Locations

• Arbaminch – is located in the Gamo Gofa Zone of the Southern Nations,

Nationalities, and Peoples Region about 500 kilometres south of Addis Ababa, at an elevation of 1285 meters above sea level.

– Geographical coordinates: 6° 2' 0" North, 37° 33' 0" East.

– The soil is characterized in clay loam texture.

• Wondo genete – is located in the Sidama Zone of the Southern Nations, Nationalities,

and Peoples Region about 300 kilometres South of Addis Ababa,

– at an elevation of 1760 meters above sea level.

– The Geographical coordinates: ??

– The soil is characterized in loam texture.

MARS population early growth

MARS population evaluated by Professors from University of Free

State and Dr Beebe

Support from GCP

• Irrigation system

Support from GCP

• Technician training on farm machinery

Acknowledgments

• GCP

• CIAT

• Dr Steve Beebe

• Dr Hannibal M from AgriconInternational

• Jake Bentum

• Dr Bodo

• Gillian Summers

• SARI