Thyroid Autoimmune Diseases:

Mechanism of development of Autoimmune endocrine disease:Two factors could be involved in development of human autoimmune disorders: 1-Expression of Class II HLA (human leukocyte antigen) on the surface of target endocrine cells. Infectious agent Inflammatory cells chemotaxis(INFγ) (or Self-antigen) Expression of HLA Presentation of own cellular proteins; reactive T and B cell response.

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2-The antigen Cross-reactivity: external organic material or infectious agents epitopes Show antigenic cross- reactivity with self tissues

formation of Auto-reactive Humoral immune response antibodies. Tissue destruction.

Chronic lymphocytic Thyroiditis:(Hashimoto’s thyroiditis):

Definition: -It is an autoimmune disease in which the thyroid gland is attacked by a variety of cell- and antibody-mediated immune processes. -The first disease recognized as autoimmune disease.-Described by the Japanese specialist (Hakaru Hashimoto) in Germany in 1912.-Hypothyroidism, large and lobulated thyroid gland.-Enlargement of thyroid due to lymphocytic infiltration and fibrosis.

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General considerations: -Family history of thyroid disease and HLA gene polymorphism (DR3, DR4, DR5).-CTLA-4 (Cytotoxic T-Lymphocyte gene A-4) Polymorphism; results in reduced negative regulation of T-lymphocytes.-Studies on Monozygotic twins show: Anti-Thyroid Antibodies (Gene homology of 80%).

Other Risk factors: -Infectious agents : Human Herpes Virus-6 (A, and B).-Chromosomal disorders: Turner ,Klinefelter’s, and Down’s Syndrome.- Pollutants (Tobacco smoke).

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-Most common in middle-aged and starts in adulthood. - 5-10 times more common in woman than in men.

-Associated with other autoimmune diseases such as: Systemic lupus erythematosus, dermatitis, and scleroderma.

Major Immunologic features of Hashimoto’s thyroiditis:

1- Lymphocytic infiltration of the thyroid gland.

2- Presence of antibodies against thyroid antigens.

3- Cellular sensitization to thyroid antigens.

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Pathogenesis: - Expression of MHC Class II-self epitope complex on the thyroid cuboidal cell surface. - Thyroid cell-CD4+ Lymphocyte interaction. - Chemotaxis of CTL and Macrophage.

- Loss of T lymphocyte suppressor function due to CTL gene A mutation. - CTL-Thyroid cell interaction and killing of target cell by Apoptosis.

-Engulfment of cellular peptide by macrophage; Ag presentation.

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- Formation of Anti- thyroid, and Anti-thyroglobulin Antibodies.

-ADCC for cuboidal cells lining the thyroid follicles by CD8 and N.K. cells.

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Histologically:

-Diffuse parenchymal infiltration of lymphocyte; mainly B-

lymphocytes which can be seen as secondary lymphoid

follicles (Germinal center).-Hǖrthle cell ( Kari Hurthle) could be seen in tissue or cytology. -Hurthle cell: metaplasia from cuboidal cells lining the thyroid follicles characterized by eosinophilic-granular cytoplasm.

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Germinal center: lymphocyte infiltrate.Pink reactive dying thyroid cell : Immunohistochemistry for P63. with cytoplasmic-acidophilic granules. :Positive in Germinal center (H.T).

Clinical Features of Chronic thyroiditis:

Primary stage: - Clinical hyperthyroidism due to inflammatory

breakdown of thyroid follicles ( Silent, Painless inflammation).

Late stage:- Hypothyroidism due to progressive destruction of

thyroid tissue and cellular malfunction.

- The last outcome of Hashimoto’s disease is the hypothyroidism.

Clinical presentation of Chronic thyroiditis:

- A consistent physical sign seen in Hashimoto’s disease is an enlarged thyroid gland (Goiter).- Enlarged surrounding lymph nodes.- Weight gain, muscle weakness, cold intolerance, depression,

fatigue, constipation, and dry skin.

- Rarely, Symptoms of generalizedvasculitis with urticaria and nephritiscould be seen due to presence ofcirculating immune complexes.

Diagnosis of Chronic thyroiditis:

- The hallmark of the diagnosis of this disease is the presence of circulating Autoantibodies:1- Anti-thyroglobulin antibodies.2- Anti-thyroid peroxidase antibodies.

- These antibodies show a sensitivity of 90% and detected by: 1- Immunofluorescence assay. 2- ELISA or Agglutination assay.

- In Seronegative patients , autoantibodies are localized in intrathyroidal lymphoid follicles.

Graves’ Disease: Definition:

-It is an autoimmune disease where the thyroid is

activated by anti-TSH receptor autoantibodies to produce

excessive amount of thyroid hormones.

-The most common cause of hyperthyroidism (60-90% of

all cases).

-It has a powerful hereditary component, affects up to 2%

of the female population, and is between five and ten

times as common in females as in males.

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General Considerations: -Hyperthyroidism and thyrotoxicosis with a diffuse goiter. - About 30-50% of people with Graves' disease will also suffer from Graves' ophthalmopathy caused by inflammation of the eye muscles by attacking autoantibodies.

Exophthalmos: upper eyelid retraction, edema, erythema, and Graves’ Goiter: hyperthyroidism conjunctivitis.

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-Specific cross-reactivity between some microbes (Viruses;

Coxsackievirus, and bacteria; Yersinia enterocolitica) and

TSH-Receptor of thyroid follicular cells.

-Family History:

The disease is associated with different types of generalized

autoimmune susceptibility; such as Hashimoto’s disease and

antibodies to gastric intrinsic factors.

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Clinical presentation:

-Goiter, exophthalmos(30-50%), muscle weakness, weight loss,

diarrhea and frequent defecation, hyperactivity ,tachycardia,

hair loss, and Oligomenorrhea.

Major Immunologic features of Graves’ disease:

1-Antibodies against thyroid antigen (TSH-R) are present;

that stimulate thyroid cell function.

2-Class II HLA expression on the surface of thyroid cells.

3-Associated autoimmune ophthalmopathy.

Graves’ disease Pathogenesis :

-Autoantibodies present against TSH-receptor: 1- Thyroid-stimulating immunoglobulins (TSI): Activate TSH-receptor; elevated thyroid hormones.2- Thyroid growth immunoglobulins( TGI) : Growth of thyroid follicles. 3-Thyroid binding-inhibiting immunoglobulins (TBII) : Inhibits TSH binding.-No Cellular immune response; Histology show no destruction of thyroid tissues.-Colloid suspension show lymphocytic infiltration: CD4, CD8, and B lymphocytes.

Pathogenesis mechanism of Graves’ disease: N

Diagnosis of Graves’ disease: -Clinically: Signs and symptoms of hyperthyroidism. -Radiology: Increased uptake of radioactive iodine.

-Serology: A-Elevated Total and free T4, and T3. B-Identification of Anti-thyroid antibodies in patient’s sera: 1-Antibodies that activate cellular cAMP ; Thyroid stimulating Immunoglobulin (TSI). 2-Thyroid growth stimulating immunoglobulins (TGI). 3-Antibodies that displace the binding of TSH from its receptor (TBII).

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Anti-thyroid antibodies could be detected by:1-ELISA Test: Microtiter plate wells should be coated by recombinant Human TSH-receptors.2-Tissue culture (Fisher Rat thyroid cell line 5): -It can be used to measure the presence and activity of Anti-thyroid antibodies ( IgG) in patient's sera. -Serum specimens should be incubated with cell line culture; then the incorporation of radioactive thymidine are measured.

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