Thin Layer Chromatography (TLC) Plates - Sigma-Aldrich ... · PDF fileWe have a range of high...

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High Performance TLC Plates Standard TLC Plates Preparative TLC Plates Other TLC Products Unfold a Variety of Trusted Options Thin Layer Chromatography (TLC) Plates

Transcript of Thin Layer Chromatography (TLC) Plates - Sigma-Aldrich ... · PDF fileWe have a range of high...

Page 1: Thin Layer Chromatography (TLC) Plates - Sigma-Aldrich ... · PDF fileWe have a range of high performance TLC (HPTLC) ... in thickness. Our standard TLC ... Thin Layer Chromatography

High Performance TLC Plates

Standard TLC Plates

Preparative TLC Plates

Other TLC Products

Unfold a Variety of Trusted Options

Thin Layer Chromatography(TLC) Plates

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TLC Plates

Support/Backing Advantages Disadvantages

Glass • Rigid

• Transparent

• Economical (reusable)

• High chemical resistance

• Most commonly used support

• Good heat stability for charring

• Fragile

• Cannot be easily cut into desired size

• Heavy – high transport costs

• Thick (>1.0 mm) – about 5x more shelf space than aluminum or plastic-backed plates

• Backing highly susceptible to breakage (potential safety issue)

Aluminum Foil • Easy to handle/safe – resistant to breakage

• Can be easily cut to desired dimensions with scissors

• Thin (~0.15 mm) – minimal storage space

• Lightweight – lower shipping costs

• Solvent resistance

• Strong adsorbent layer adherence – good for use with eluents containing high concentrations of water

• Good heat stability

• Can be stored in lab notebook

• Backing is not reusable

• Not as chemically resistant as glass to reagents such as mineral acids and concentrated ammonia

Plastic (Polyester – PET) • Easy to handle/safe – resistant to breakage

• Can be easily cut to desired dimensions with scissors

• Thin (~0.2 mm) – minimal storage space

• Lightweight – lower shipping costs

• Solvent resistance

• Can be stored in lab notebook

• Backing is not reusable

• Lower heat stability – charring must be done at lower temperatures than with glass

• Flexible – adsorbent layer may be more susceptible to cracking

SupportsThree different types of supports (or backings) are available to meet your individual application requirements. The key advantages and disadvantages of each support are identified in the table below:

Ensure Selection, Quality and Performance

BrandsWe offer our own high quality brand of thin layer chromatography (TLC) plates as well as brand name TLC plates from EMD Millipore and Analtech to meet our customers’ needs for brand name, quality and price.

Plate TypesWe have a range of high performance TLC (HPTLC) plates as well as an extensive offering of standard analytical and preparative TLC plates.

Thin Layer Chromatography (TLC) Plates

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3TLC Plate Features

Adsorbent Layers/Matrices

Our glass, aluminum foil and PET (polyester) TLC plates are available coated with a variety of matrices and are available with and without fluorescent indicator.

Available matrices include:

• Silica gel (unmodified, modified/bonded, chiral and high purity) – most common TLC sorbent. Eluents used are similar to those used for normal phase HPLC. Does not have the catalytic properties of alumina.

• Aluminum oxide – exhibits selectivity similar to, although slightly different than, silica. Second most common TLC sorbent.

• Cellulose – available as either microcrystalline or fibrous cellulose. Spots are generally more compact when separated on layers of microcrystalline cellulose than when separated on layers of fibrous cellulose.

• Polyamide – exhibits low diffusivity, allowing for tightly confined sample spots with maximum intensity. Corrosive visualization reagents are not recommended.

BindersPlates are available with polymeric (organic) and inorganic binders as well as without binders. Binders do not generally impact the adsorptive properties of the matrix, instead they impact the adherence of the adsorbent layer to the backing.

Available binders include:

• Polymeric (organic) binders – Plates with adsorbent layers containing polymeric binders are generally most rugged, making sample handling and application easier. They also permit the use of higher water content in the developing solvent. They are generally recommended for all TLC applications except for those that use charring for visualization.

• Inorganic binders – Plates with adsorbent layers containing inorganic binders are not as rugged as those with organic binders but are generally more rugged than plates without a binder or with a gypsum binder. They are the most water resistant and compatible with aqueous developing reagents. Inorganic binders are often used for preparative TLC plates because they are soft, allowing for spots containing the target molecule to be easily scraped off the support for elution and recovery. Plates with inorganic binder are recommended for applications requiring charring for visualization, or when developing or visualizing reagents interact with an organic binder.

• Gypsum or no binder – Plates with adsorbent layers that do not contain a binder or that use a gypsum binder are generally least rugged and fractured easily. They are not recommended for use with developing solvents containing more than 20% water. Like plates with inorganic layers, they allow spots containing the target molecule to be easily scraped off the support for elution and recovery and are recommended for applications requiring charring for visualization.

Plate Dimensions

Choose from a comprehensive variety of plate dimensions:

• Larger plates (such as 20 cm x 20 cm plates) are often less costly as they can be cut into smaller sizes as needed.

• Prescoring (available on some of our glass plates) makes cutting glass plates easier and safer as they can be easily snapped into smaller dimensions.

• Aluminum and plastic (PET)-backed plates are easy to cut to desired dimensions with a pair of scissors.

• Smaller, precut plates are also available and offer the advantages of convenience and ease of use that may make them the more practical choice in labs where productivity rather than per plate cost is the major driver.

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Thin Layer Chromatography (TLC) Plates

HPTLC PlatesAchieve Faster Separations and Increased Sensitivity HPTLC (high performance TLC) plates are characterized by smaller particles (≤10 μm), thinner layers (≤150 μm) and smaller plates (≤10 cm developing distance). In addition, the particle size distribution of the sorbent is typically narrower than for standard TLC layers. The thinner layers allow faster solvent migration. Sample volumes for HPTLC are generally less than one microliter. The smooth surface of HPTLC plates reduces noise when performing densitometric quantitation, increasing sensitivity.

HPTLC plates offer users the following advantages over conventional TLC:

• Better resolving power per unit distance

• Faster development times

• Lower sample diffusion

• Reduced solvent consumption

HPTLC Plates With Glass Backing

Silica on Glass HPTLC Plates

Indicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.10 cm x 10 cm plates (scored)Yes 150 Inorganic 25 Analtech Z265330-1PAK10 cm x 10 cm platesNo 150 Organic 25 Analtech Z265292-1PAKNo 150 Inorganic 25 Analtech Z265314-1PAKYes 150 Organic 25 Analtech Z265306-1PAKYes 150 Inorganic 25 Analtech Z265322-1PAK10 cm x 10 cm platesNo 200 Polymeric 25 EMD Millipore Z740222-25EAYes 200 Polymeric 25 EMD Millipore Z740223-25EAYes 200 Polymeric 100 EMD Millipore Z740224-100EA10 cm x 20 cm platesYes 200 Polymeric 50 EMD Millipore Z740220-50EANo 200 Polymeric 50 EMD Millipore Z740221-50EA

C18-Silica on Glass HPTLC Plates

Indicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.10 cm x 10 cm platesNo 150 Organic 25 Analtech Z265365-1PAKYes 150 Organic 25 Analtech Z265373-1PAK10 cm x 10 cm plates (50% silanized)Yes 200 Organic 25 Analtech Z265411-1PAK10 cm x 10 cm plates (high % carbon)No 150 Organic 25 Sigma-Aldrich® Z122750

C8-Silica on Glass HPTLC Plates

Indicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.10 cm x 10 cm platesYes 150 Organic 25 Analtech Z265403-1PAK

Like our standard analytical TLC plates, our HPTLC plates are available coated with a variety of matrices, with and without indicators.

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5HPTLC Plates

Amino-Silica on Glass HPTLC PlatesIndicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.10 cm x 10 cm platesYes 150 Organic 25 Analtech Z265349-1PAK

Cyano-Silica on Glass HPTLC PlatesIndicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.10 cm x 10 cm platesYes 150 None 25 Analtech Z265357-1PAK

Cellulose (Fibrous) on Glass HPTLC PlatesIndicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.10 cm x 20 cm platesNo 100 Proprietary 50 Sigma-Aldrich 95412-50EA20 cm x 20 cm platesYes 100 Proprietary 25 Sigma-Aldrich 73128-25EA

HPTLC Plates With Aluminum Foil Backing

Nano Silica on Aluminum Foil HPTLC PlatesIndicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.20 cm x 20 cm platesYes 150 Organic 25 Sigma-Aldrich 60761-25EA

Cellulose (Fibrous) on Aluminum Foil HPTLC PlatesIndicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.10 cm x 20 cm platesYes 100 Proprietary 20 Sigma-Aldrich 22185-20EA-F20 cm x 20 cm platesNo 100 Proprietary 25 Sigma-Aldrich 95413-25EA

Silica on Aluminum SheetsIndicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.20 cm x 20 cm platesNo 250 Polymeric 25 EMD Millipore Z740232-25EA

C18 on Aluminum SheetsIndicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.20 cm x 20 cm platesYes 250 Polymeric 20 EMD Millipore Z740234-20EA

HPTLC Plates With Plastic (PET) Backing

Cellulose on Plastic (PET) HPTLC PlatesIndicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.20 cm x 20 cm platesNo 100 None 25 Sigma-Aldrich Z122866-25EA20 cm x 20 cm plates (PEI-cellulose)Yes 100 Polymeric 25 Sigma-Aldrich Z122882-25EA20 cm x 20 cm plates (microcrystalline cellulose)Yes 100 Proprietary 25 Sigma-Aldrich 95725-25EA

Polyamide on Plastic (PET) HPTLC PlatesIndicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.20 cm x 20 cm platesNo 100 Proprietary 25 Sigma-Aldrich 17288-25EAYes 100 Proprietary 25 Sigma-Aldrich 17289-25EA

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TLC Plates With Glass Backing

Silica (High Purity) on Glass

Indicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.5 cm x 10 cm platesNo 250 Gypsum/polymeric 50 Sigma-Aldrich® 60762-50EAYes 250 Gypsum/polymeric 50 Sigma-Aldrich 60763-50EA20 cm x 20 cm plates (with concentration zone)Yes 250 Gypsum/polymeric 25 Sigma-Aldrich 60768-25EA20 cm x 20 cm plates (w/ gypsum)Yes 250 Gypsum/polymeric 25 Sigma-Aldrich 08572-25EA-F

Silica on Glass Without Indicator

Indicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.5 cm x 20 cm platesNo 250 Polymeric 100 Sigma-Aldrich Z122688-100EANo 250 Inorganic 25 Analtech Z265608-1PAKNo 250 Organic 25 Analtech Z265535-1PAK10 cm x 10 cm platesNo 200 Organic 25 Sigma-Aldrich 09918-25EA10 cm x 20 cm platesNo 250 Polymeric 50 Sigma-Aldrich Z185310-50EANo 250 Polymeric 50 EMD Millipore Z292966-1PAKNo 250 Inorganic 25 Analtech Z265586-1PAKNo 250 Organic 25 Analtech Z265519-1PAK10 cm x 20 cm plates (impregnated with caffeine; for PAH analytics (DIN38407, part 7)No 200 Proprietary 50 Sigma-Aldrich 60796-50EA20 cm x 20 cm platesNo 250 Polymeric 25 Sigma-Aldrich 99570-25EANo 250 Polymeric 25 EMD Millipore Z292974-1PAKNo 250 Inorganic 25 Analtech Z265551-1PAKNo 250 Organic 25 Analtech Z265497-1PAK

Standard TLC PlatesDevelop the Method and Verify Results Thin layer chromatography can be used for both qualitative and quantitative analysis. Standard analytical TLC plates typically have adsorbent layers that are nominally between 200 and 250 microns in thickness. Our standard TLC plates are available coated with a variety of matrices, with and without indicators.

Thin Layer Chromatography (TLC) Plates

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7Standard TLC Plates

Silica on Glass With IndicatorIndicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.2.5 cm x 7.5 cm platesYes 250 Polymeric 100 EMD Millipore Z740213-100EA Yes 250 Polymeric 500 EMD Millipore Z740214-500EA5 cm x 10 cm platesYes 250 Polymeric 200 EMD Millipore Z292990-1PAK5 cm x 20 cm platesYes 250 Polymeric 100 Sigma-Aldrich 99569-100EAYes 250 Polymeric 100 Sigma-Aldrich Z122696-100EAYes 250 Inorganic 25 Analtech Z265616-1PAKYes 250 Organic 25 Analtech Z265543-1PAK10 cm x 10 cm platesYes 250 Polymeric 25 Sigma-Aldrich 99573-25EAYes 200 Organic 25 Sigma-Aldrich 09916-25EA10 cm x 20 cm plates (scored)Yes 250 Inorganic 25 Analtech Z274283-1PAK10 cm x 20 cm plates Yes 200 Organic 50 Sigma-Aldrich 09922-50EAYes 250 Polymeric 50 Sigma-Aldrich 99876-50EAYes 250 Polymeric 50 EMD Millipore Z293016-1PAKYes 250 Organic 25 Analtech Z265527-1PAK10 cm x 20 cm plates (w/ concentration zone)Yes 250 Proprietary 50 Sigma-Aldrich 60767-50EA20 cm x 20 cm platesYes 250 Polymeric 25 Sigma-Aldrich 99571-25EAYes 250 Polymeric 25 Sigma-Aldrich Z122726-25EAYes 250 Polymeric 25 EMD Millipore Z293024-1PAKYes 250 Inorganic 25 Analtech Z265578-1PAKYes 250 Organic 25 Analtech Z265500-1PAK

C18-Silica on Glass Without IndicatorIndicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.5 cm x 20 cm platesNo 250 Inorganic 25 Analtech Z265470-1PAK10 cm x 20 cm platesNo 250 Inorganic 25 Analtech Z265454-1PAK20 cm x 20 cm plates (scored)No 250 Inorganic 25 Analtech Z500666-1PAK20 cm x 20 cm platesNo 250 Inorganic 25 Analtech Z265438-1PAK

C18-Silica on Glass With IndicatorIndicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.5 cm x 20 cm platesYes 250 None 25 Analtech Z265489-1PAK10 cm x 10 cm platesYes 250 Organic 25 Sigma-Aldrich 99575-25EA10 cm x 20 cm plates (scored)Yes 250 None 25 Analtech Z500992-1PAK10 cm x 20 cm platesYes 250 None 25 Analtech Z265462-1PAK10 cm x 20 cm plates (w/ low %C)Yes 250 Polymeric 50 Sigma-Aldrich® Z292923-1PAK20 cm x 20 cm plates (scored)Yes 250 None 25 Analtech Z500887-1PAK20 cm x 20 cm platesYes 250 None 25 Analtech Z265446-1PAKYes 250 Polymeric 25 EMD Millipore Z293032-1PAK

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Chiral Silica on GlassIndicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.5 cm x 20 cm platesYes 250 Organic 50 Sigma-Aldrich 99697-50EA10 cm x 20 cm platesNo 250 Polymeric 4 Sigma-Aldrich Z148687-4EA20 cm x 20 cm platesNo 250 Polymeric 25 Sigma-Aldrich Z148717-25EA

Cellulose (Fibrous) on GlassIndicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.10 cm x 20 cm platesNo 250 Proprietary 50 Sigma-Aldrich Z122815-50EAYes 250 Proprietary 50 Sigma-Aldrich Z122823-50EA20 cm x 20 cm platesNo 250 Proprietary 25 Sigma-Aldrich Z122831-25EAYes 250 Proprietary 25 Sigma-Aldrich Z122858-25EA

Cellulose (Microcrystalline) on GlassIndicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.5 cm x 20 cm platesYes 250 None 25 Analtech Z265799-1PAK20 cm x 20 cm plates (scored)Yes 250 None 25 Analtech Z513083-1PAK20 cm x 20 cm platesNo 250 None 25 Analtech Z265764-1PAKYes 250 None 25 Analtech Z265772-1PAKYes None None 25 EMD Millipore Z740215-25EA

Aluminum Oxide on GlassIndicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.5 cm x 20 cm platesNo 250 Inorganic 25 Analtech Z265659-1PAKYes 250 Inorganic 25 Analtech Z265667-1PAK10 cm x 20 cm plates (scored)Yes 250 Inorganic 25 Analtech Z500550-1PAK20 cm x 20 cm platesNo 250 Organic 25 Sigma-Aldrich 02665-25EAYes 250 Organic 25 Sigma-Aldrich 90066-25EANo 250 Inorganic 25 Analtech Z265632-1PAKYes 250 Inorganic 25 Analtech Z265640-1PAK

TLC Plates With Aluminum Foil BackingHigh Performance Silica on Aluminum Foil

• Outstanding wettability (even for 100% aqueous visualization reagents)

• Easy and precise cutting (no flaking of silica)

• Excellent separation efficiency and reproducibility from batch-to-batch

Indicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.4 cm x 8 cm platesYes 200 Polymeric 50 Sigma-Aldrich 49859-50EA5 cm x 7.5 cm platesYes 200 Polymeric 20 Sigma-Aldrich 52038-20EANo 200 Polymeric 20 Sigma-Aldrich 55811-20EA5 cm x 10 cm platesYes 200 Polymeric 50 Sigma-Aldrich 23478-50EANo 200 Polymeric 50 Sigma-Aldrich 75196-50EA5 cm x 20 cm platesYes 200 Polymeric 50 Sigma-Aldrich 12606-50EANo 200 Polymeric 50 Sigma-Aldrich 92572-50EA

Thin Layer Chromatography (TLC) Plates

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9Standard TLC Plates

Indicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.20 cm x 20 cm platesYes 200 Polymeric 25 Sigma-Aldrich 56524-25EANo 200 Polymeric 25 Sigma-Aldrich 53356-25EA

Silica on Aluminum FoilIndicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.4 cm x 8 cm platesYes 200 Polymeric 50 Sigma-Aldrich 70643-50EA5 cm x 7.5 cm platesYes 200 Polymeric 20 EMD Millipore Z740231-20EA5 cm x 10 cm platesYes 200 Polymeric 50 Sigma-Aldrich 91835-50EAYes 200 Polymeric 50 Sigma-Aldrich Z193275-1PAKNo 200 Polymeric 50 Sigma-Aldrich 70644-50EA5 cm x 20 cm platesYes 200 Polymeric 100 EMD Millipore Z740229-100EA10 cm x 20 cm platesYes 200 Polymeric 20 Sigma-Aldrich 60800-20EANo 200 Polymeric 20 Sigma-Aldrich 02599-20EA20 cm x 20 cm platesYes 200 Polymeric 25 Sigma-Aldrich 60778-25EANo 200 Polymeric 25 Sigma-Aldrich 60805-25EAYes 200 Polymeric 25 Sigma-Aldrich Z193291-1PAKYes 200 Polymeric 25 EMD Millipore Z740226-25EANo 200 Polymeric 25 EMD Millipore Z740227-25EA

Aluminum Oxide on Aluminum FoilIndicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.20 cm x 20 cm platesYes 200 Organic 25 Sigma-Aldrich 06408-25EA

Silica Gel 60 W*Indicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.20 cm x 20 cm platesYes None Polymeric 25 EMD Millipore Z740230-25EA

*Water Resistant

Aluminum Oxide 60 on Aluminum Sheets

Indicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.20 cm x 20 cm platesYes 200 None 25 EMD Millipore Z740228-25EA

TLC Plates With Plastic (PET) BackingSilica (High Purity) on Plastic (PET)

Indicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.20 cm x 20 cm platesYes 250 Gypsum/polymeric 25 Sigma-Aldrich Z122807-25EA

Silica on Plastic (PET)

Indicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.4 cm x 8 cm platesYes 250 Proprietary 50 Sigma-Aldrich® 89070-50EA20 cm x 20 cm platesYes 200 Proprietary 25 Sigma-Aldrich 99577-25EA

High Performance Silica on Aluminum Foil (continued)

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Indicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.No 250 Polymeric 25 Sigma-Aldrich Z122777-25EAYes 200 Polymeric 25 Sigma-Aldrich Z122785-25EAYes 200 Polymeric 25 EMD Millipore Z740235-25EANo 200 Polymeric 25 EMD Millipore Z740236-25EA

Aluminum Oxide on Plastic (PET)

Indicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.4 cm x 8 cm platesYes 200 Organic 50 Sigma-Aldrich 89071-50EA20 cm x 20 cm platesNo 200 Organic 25 Sigma-Aldrich Z234206-1PAKYes 200 Organic 25 Sigma-Aldrich Z234214-1PAK

PEI Cellulose on Plastic (PET) Backing

Indicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.20 cm x 20 cm platesYes None None 25 EMD Millipore Z740237-25EA

Thin Layer Chromatography (TLC) Plates

Preparative TLC PlatesIsolate and Recover Your CompoundPreparative TLC is used to purify and isolate a particular substance by separating it from contaminants. Preparative TLC plates have adsorbent layers that are greater than 500 microns in thickness allowing them to be used for the separation of larger quantities of material. When a binder is required, the softer inorganic binders are often used so the sample bands can be removed more easily. Our glass plates for preparative TLC are available coated with both unmodified and bonded silica matrices and are available with and without fluorescent indicator.

Silica on Glass Prep TLC Plates

Indicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.20 cm x 20 cm plates No 500 Inorganic 25 Analtech Z512990-1PAKYes 500 Inorganic 25 Analtech Z513032-1PAKNo 1,000 Inorganic 25 Analtech Z265810-1PAKYes 1,000 Inorganic 25 Analtech Z265829-1PAKNo 1,500 Inorganic 25 Analtech Z513016-1PAKYes 1,500 Inorganic 25 Analtech Z513040-1PAKNo 2,000 Inorganic 25 Analtech Z513024-1PAKYes 2,000 Inorganic 25 Analtech Z513059-1PAKYes 500 Polymeric 20 EMD Millipore Z740218-20EAYes 1000 Polymeric 15 EMD Millipore Z740216-15EAYes 2000 Polymeric 12 EMD Millipore Z740219-12EA

C18-Silica on Glass Prep TLC Plates

Indicator Layer Thickness (µm) Binder Plates/Pack Brand Cat. No.20 cm x 20 cm plates Yes 1,000 Inorganic 25 Analtech Z272426-1PAK

Silica on Plastic (PET) (continued)

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11Other TLC Products

Other TLC ProductsSupport Your Entire WorkflowIn addition to our diverse offering of TLC plates, we also provide other products to support your entire TLC workflow, including products for plate spotting and development, visualization and sample recovery and storage.

Plate Spotting and Development

UV Lamps and Cabinets

Sample Recovery and Storage

• Scrapers

• Plate cutters/scoring tools

• Plate storage racks

• Sample recovery tubes

• Dessicating cabinets

• Spotting syringes and micro capillary pipettes

• Plate holders

• Development tanks and chambers

• Solvents

Visualization

• Sprayers – We offer both flask-type and bottle-type TLC sprayers in a variety of sizes ranging from 10 to 250 mL.

• Spray Reagents – Spray reagents allow visualization of the colorless components on a TLC plate. These reagents are mixtures of chemicals that, when sprayed on the plate and allowed to react, will cause colorless compounds to change to colored compounds for easy detection. See the table below for a sampling of spray reagents we offer.

Product Description Pkg. Cat. No.Phosphomolybdic AcidDetection of lipids, steroids, lactones, keto acids, hydroxy acids, unsaturated fatty acids and phenolic compounds

100 mL P4869

NinhydrinDetection of amino acids, amines and amino sugars 100 mL N1286IodoplatinateDetection of alkaloids, amines and organic nitrogen compounds

100 mL I9157

MolybdenumDetection of phospholipids and related compounds 100 mL M1942

Scan to visit our TLC resources, or visit sigma-aldrich.com/TLC

We offer products that range from hand-held units to bench-top cabinets with a variety of features to meet your specific needs.

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