Thesis Proposal.

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In vivo determination of the hypoglycemic effect of Mollugo oppositofollia Linn. (Molluginaceae) plant extracts in Alloxan- induced male Sprague- Dawley rats HERNANDEZ, RAY CONRAD. , JAVIER, JEZREEL ROLLAN., LIM, ALEC JACOB, MANUEL, ROSELLE, PENSOTES, DARRYL VAN , RAMORAN, EDRICK GLENN DR. GAVINO IVAN TANODRA University of Santo Tomas Faculty of Pharmacy 1

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Mollugo Oppositifolia Extraction

Transcript of Thesis Proposal.

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In vivo determination of the hypoglycemic effect of Mollugo oppositofollia Linn. (Molluginaceae) plant extracts in Alloxan-induced male Sprague-Dawley ratsHERNANDEZ, RAY CONRAD. , JAVIER, JEZREEL ROLLAN., LIM, ALEC JACOB, MANUEL, ROSELLE, PENSOTES, DARRYL VAN , RAMORAN, EDRICK GLENN

DR. GAVINO IVAN TANODRA

University of Santo TomasFaculty of Pharmacy

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Introduction

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3Diabetes Mellitus

Disorder in which blood sugar (glucose) levels are abnormally high.

Metabolic diseases characterized by hyperglycemia Defects in the B-cells of the pancreas

(American Diabetes Association, 2003)

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4Type 1 Diabetes

insulin-dependent diabetes juvenile-onset diabetesautoimmune destruction of the β-cells of

the pancreas

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5Insulin

hormone released from the β-cells of pancreas controls the amount of glucose in the blood

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6Prevalence of Diabetes According to the International Diabetes

Federation & World Health Organization 387 million people around the world have diabetes cause of 1.5 million deaths and is projected to rise

by more than 50% in the next 10 years 7th leading cause of death in the world

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7Alloxan 2,4,5,6- pyrimidinetetrone diabetogenic agent induce Type 1 diabetes in experimental

animals administered parenterally

intravenously intraperitoneally subcutaneously.

Usual dose is 65 mg/kg

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8Mollugo oppositofollia Linn. : Botanical Description

Kingdom: Plantae

Phylum: Tracheophyta

Class: Magnoliospida

Order: Caryophyllales

Family: Molluginaceae

Genus: Mollugo

Species: Mollugo oppositofolia

 

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9Mollugo oppositofollia Linn. : Botanical Description

Characterized by diffuse prostrate, annual herb

Made up of numerous, dichotomously branched slender stems.

Possesses 13-22 mm long leaves, in whorls of 4-5, unequal, and oblanceolate

Consists of small, white flowers, in axillary fascicles of 2 or more, with long filiform pedicels

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10Mollugo oppositofollia Linn.: Botanical Description

applied topically for the treatment of skin diseases and itch

used with castor oil as treatment for ear ache natural source of antioxidant

Mollugo oppositofollia Linn.

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Problem Rationale

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13Objectives

To determine which fraction will elicit the highest hypoglycemic activity.

To identify the constituents present in the different fractions.

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14Scope and Limitations Determination of efficacy by decreasing blood

glucose level using a glucometer Toxicity will not be performed Lingayen Male Sprague-dawley rats will be used Diabetes induction by use of Alloxan Soxhlet

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15Significance of the Study

To serve as a tool for application of what the researchers learned in the field of pharmacy.

To serve as a reference for future hypoglycemic studies.

To provide information about the hypoglycemic effect of Mollugo oppositifolia Linn.

Best?

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Research Design

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Collection of Mollugo oppositifolia Linn. plant sample

Research Design

Identification of the wholeplant sample

Drying of the plant sample

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Grinding of the plant sample

Extraction with hexane, chloroform, 2-butanol, methanol

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Rotary Evaporator

Soxhlet Apparatus

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Phytochemical Analysis

IACUC Application

Induction of diabetes to male Sprague- Dawley rats

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21Group I: Normal healthy control animals

(fed with normal chow and water)Group II: Diabetes induced control

animals treated with alloxan monohydrate in sterile saline (150 mg/kg by i.p. injection, single dose)

Group III: Diabetes induced animal treated with hexane extract (500 mg/kg/day orally for 35 days)

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22Group IV: Diabetes induced animal

treated with chloroform extract (500 mg/kg/day orally for 35 days)

Group V: Diabetes induced animal treated with 2-butanol extract (500 mg/kg/day orally for 35 days)

Group VI: Diabetes induced animal treated with methanol extract (500 mg/kg/day orally for 35 days)

Group VII: Drug control-animals, diabetes induced rats treated with Insulin (1 U/200 g /day intraperitonially for 35 days)

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Administration of crude extracts

Biochemical Analysis

Statistical Analysis

Glucometer

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25REFERENCESThe Wonders of ‘Guyabano’ and Other Philippine Medicinal Plants Documented in New

Book. (2014, November 4). Philippine Daily Inquirer.

Rizvi, S.I., & Mishra, N. (2013). Traditional Indian Medicine Used for the Management of Diabetes Mellitus. Journal of Diabetes Research. doi:10.1155/2013/712092

Okamoto, M.M., et. al. (2011). Intensive Insulin Treatment Induces Insulin Resistance in Diabetic Rats by Impairing Glucose metabolism-related Mechanism in Muscle and Liver. J Endocrinol, 211(1), 55-64. doi: 10.1530/JOE-11-0105

Skak, K., Gotfredsen, C.F., Lundsgaard, D., Hansen, J.B., Sturis, J., Markholst, H. (2004). Improved β-Cell Survival and Reduced Insulitis in a Type 1 Diabetic Rat

Model After Treatment with a β-Cell-Selective KATP Channel Opener. Diabetes, 53(4), 1089-1095. doi:10.2337/diabetes.53.4.1089

Rizvi, S.I., Matteucci, E., and Atukeren, P. (2013). Traditional Medicine in Management of Type 2 Diabetes Mellitus. Journal of Diabetes Research. doi:10.1155/2013/58082

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26Choi, H.Y., et. al. (2014). Effects of food on the pharmacokinetics of a gemigliptin/metformin sustained-release 50/1,000 mg (25/500 mg x 2 tablets)

fixed-dose combination tablet in healthy male volunteers. International Journal of Clinical Pharmacology and Therapeutics, 52(5), 381-391.

Wilkins, J., Dubar, M., Sebastien, B., and Laveille, C. (2013). A Drug and Disease Model for Lixisenatide, a GLP-I Receptor Agonist in Type 2 Diabtetes. The Journal of Clinical Pharmacology, 54(3), 267-278.

Modak, M., Dixit, P., Londhe, J., Ghaskadbi, S., and Devasagayam, T.P.A. (2007). Indian Herbs and Herbal Drugs Used for the Treatment of Diabetes.

Journal of Clinical Biochemistry and Nutrition, 40(3), 163-173.

World Health Organization. (2002). Traditional medicine-growing needs and potential. WHO Policy Perspective on Medicines, 2, 1-6.

Wang, Z., Wang, J., & Chan, P. (2013). Treating Type 2 Diabetes Mellitus with Traditional Chinese and Indian Medicinal Herbs. Evidence-Based Complementary and Alternative Medicine : eCAM, 2013, 343594.doi:10.1155/2013/343594

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Thank You