Thermo Scientific Evolution Array UV-Visible

Spectrophotometer

m o l e c u l a r s p e c t r o s c o p y

Part of Thermo Fisher Scientific

The Ultimate in Versatility

Quality Control and Assurance

Life Science

Materials Science

Education

A Complete Analytical Environment

Fast data acquisition and superb reliability make the Thermo Scientific EvolutionArray spectrophotometer the cornerstone of your complete analytical environment.Instant full-spectrum analysis, room light immunity, and a complete line ofaccessories deliver simplicity and capability that is second to none.

F R O M S A M P L E S T O A N S W E R S I N A N I N S T A N T

With complete, full-spectrum data acquisition and display inroughly a second, the Evolution™ Array™ provides nearlyinstantaneous answers. The reverse optical configurationallows fast data collection – dramatically increasing thethroughput of your laboratory. USB-based communicationallows the fastest transport of spectral data to the computersoftware for data analysis.

Nothing impacts the throughput of an analytical laboratorymore than a “down” instrument. With a mechanical shutteras its only moving part, and a tungsten lamp with a 10,000hour lifetime, the Evolution Array delivers longer intervalsbetween lamp changes and re-qualifications. This robustdesign assures that the instrument works longer and harder.

I N S T R U M E N T

The Evolution Array spectrophotometerfeatures a complete line of accessoriesto help you assemble the best analyticalsystem for your laboratory. Accessoriesare easy to insert and remove, allowingmulti-user labs to move quickly from oneexperiment to the next. Automated cellchangers, temperature control and solidsample accessories give you the versatilityyou need to measure difficult samplesquickly. Whether your needs are researchor routine, our accessory selectionallows you to build a customized systemthat delivers the results you demand.

A CC E S S O R YS O F T W A R E The VISIONcollect ™ software allows you to configure

customized reports for your data and analysis. Methoddetails, instrument and operator information, graphics,and report tables are seamlessly incorporated into thereport. Whether exhaustive or concise, the VISIONcollectsoftware allows you to tailor reporting to the individualneeds of each method.

Optional Thermo Scientific Security softwareenables data handling in accordance with the US FDA’s21 CFR Part 11 requirements for electronic signatures andcontrol of retained data.

R E P O R T

Thermo Scientific VISIONcollect software provides complete instrument and accessorycontrol as well as data collection, analysis, andreporting capabilities. The software features agraphical interface with toolbars for quickaccess to commonly performed functions andeasy navigation. Keyboard shortcuts allow evenfaster automation of commonly performed tasks.Applications like kinetics, quantitative analysis,and wavelength monitoring are segregated forsimplicity. All applications feature a spectrumradar visualization tool that allows you to see the entire active spectrum at a glance.

Our History of Innovat ion

Ensure the most accurate results by protecting theintegrity of your sample. Maintaining a stable envi-ronment for the sample is important for gettingaccurate results. Our versatile accessories ensureyour samples are properly maintained throughoutthe analysis process. Some liquid samples requirethe high-throughput capabilities of an autosampleraccessory while others need accurate temperaturecontrol with a Peltier cell changer. Delicate solidsamples are easily analyzed with our specular anddiffuse reflectance accessories.

S A M P L E

GENESYS 10 instrumentsintroduced – patented out-of-plane optics for superior per-formance in a small footprint

Evolution 300 introduced – first and only double-beam xenonlamp based instrument

Unicam introduces its first commercial UV-Visiblespectrophotometer – Unicam SP-500

SPECTRONIC 20 introduced –first mass produced, low-costspectrophotometer

Spectronic 2000 introduced – first micro-processor controlleddouble-beam UV-Vis

Unicam PU-8800 introduced –double monochromator UV-Visible spectrophotometer

Aminco-Bowman AB2 Fluorescencespectrometer introduced

Helios series introduced –compact, double-beamUV-Vis

Thermo Fisher Scientificacquires NanoDrop tobecome world leader inUV-Visible Spectroscopy

NanoDrop 2000 and2000c introduced furtherinnovating microvolumesampling for UV-Vis

Evolution ArraySpectrophotometerintroduced

LuminaFluorescenceSpectrometer introduced

Thermo Scientific UV-Visible and fluorescence instruments havea long history of innovation and quality. Our legacy includesfamiliar products from SPECTRONIC, Unicam, and NanoDrop.

Performance verification tests in the VISIONcollectsoftware offer preprogrammed methods for verifying instrument performance according topharmacopoeia requirements and GxP procedures.Methods are available for:

• Wavelength Accuracy and Reproducibility• Photometric Accuracy and Reproducibility• Noise and Baseline Flatness• Resolution• Stray Light at 200, 220 and 340 nm• Stability• Dark Current

Additional built-intests allow you to monitorgeneral instrument performance parameters,such as wavelength calibration, lamp usageand multicell calibration,to ensure your EvolutionArray system is alwaysfunctioning optimally.

System ValidationThe Thermo Scientific UV Validator Array packageprovides support for system qualification and validation activities for the Evolution Array spectrophotometer, software and accessories. It provides a software module and all the docu-mentation needed to facilitate compliance of yourspectrophotometer system with the requirements ofFDA, GxP, ISO 9001:2000 and ISPE 2001 guidelinesand regulations. The Validator Array streamlinesDesign (DQ), Installation (IQ), Operational (OQ) and Performance (PQ) qualification procedures for simple and efficient use by the end user.

Ensure Optimum Performance of Your System

1 9 4 0 1 9 5 3 1 9 8 0 1 9 8 2 1 9 9 1 1 9 9 4 2 0 0 0 2 0 0 3 2 0 0 7 2 0 0 9 2 0 1 0 2 0 1 0

Array TechnologyF U L L S P E C T R U M D A T A A C Q U I S I T I O N

Photodiode array technology allows you to acquire full-spectrum UV-Visibledata almost instantaneously. Increase efficiency and throughput in:

• Quality Control and Assurance measurements

• Pharmaceutical method development and deployment

• Multi-wavelength kinetics experiments with millisecond resolution

• Routine to research life science applications

• Coating and color applications

• Multi-user analytical and educational laboratories

A photodiode array based spectrophotometer providesa nearly instantaneous measurement of the entireUV-Visible spectrum. For some applications and methods, this optical configuration offers tremendousadvantages in data acquisition and sample throughputcompared to a traditional monochromator-based spectrophotometer.

Diode array instruments use a reverse opticalgeometry where light of all wavelengths is passedthrough the sample. The sample absorbs some characteristic wavelengths based on its composition.Wavelengths of light not absorbed by the sample aretransmitted to the detection system. The transmittedlight passes through an entrance slit and is then dispersed onto a photodiode array by a grating.Simultaneous detection at all wavelengths throughoutthe UV and visible regions of the spectrum allowsnearly instantaneous display of an absorption spectrum.

Traditional, monochromator-based systems measurescan speed in nanometers per minute because only a single wavelength is measured before the mono-chromator must be moved and the next wavelengthmeasured. In contrast, acquiring data in a singlemeasurement allows an equivalent scan speed of54,600 nm/min.

Further, reverse-optical systems have no movingparts; therefore they provide superior wavelengthaccuracy and repeatability. These instruments alsoneed much less maintenance and offer much longerinterval between qualifications.

...this optical configuration offers tremendous advantages in data

acquisition and sample throughput...

Whether looking at a full spectrum, monitoringseveral fixed wavelengths, performing aquantitative analysis or kinetics experiment,the Evolution Array delivers clear, accurateresults quickly.

Photodiode array detection offers superbspeed and convenience for UV-Visible measurements – instant measurements atan unlimited number of wavelengths. Thisuseful feature greatly increases samplethroughput and accelerates any analysis.

Wavelength MonitoringWavelength Monitoring Mode provides full-spectrum and fixed wavelength analysiscapabilities. Use this mode to compare thespectra of all samples on one customizablegraph. Select specific wavelengths tomonitor or find up to 20 peaks or valleys withautomated or manual peak picking options.The Wavelength Monitoring Mode also supports user-defined equations andadvanced spectral processing featuring:• Spectral addition and subtraction• Scalar addition, multiplication and division• Log• Smoothing• Derivatives

Reliable Quantitative Analyses Reliable quantitative results are an essentialcomponent of quality control analyses acrossmany disciplines, including pharmaceutical,food and beverage and specialty chemicalindustries. The Evolution Array deliversaccurate, reliable quantitative results quicklyusing single or multicomponent methods.Full-spectrum data from every standard andsample enables the user to create standardcurves and examine samples at any wave-length at any time, an invaluable tool formethod development and sample analysisactivities. Customize your quantitative methods with:• User specified concentration units• Entry of up to 100 standards• First, Second and Third Order curve fits• Zero offset for calibration curves• Calculation of %Error for calibration

diagnostics

View the full spectrum of a selected sample with the spectrumradar while simultaneously working in the main display window.

Clear and Accurate Results

Acquire high quality data fast with the Evolution Array spectrophotometer. The Evolution Array collects the full spectrum of a sample from 190 to 1100 nmand returns the results in about one second, making it ideal for routine and high-throughput applications in any laboratory.

T H E P O W E R O F F A S T F U L L S P E C T R U M D A T A A C Q U I S I T I O N

CONVENIENT LIFE SCIENCE ASSAYS

PRECISE TEMPERATURE CONTROL

FULL-SPECTRUMKINETICS

Convenient pre-defined biological methodsin the VISIONcollect Complete software provide a solid foundation for analyzingproteins and nucleic acids. Select frommethods for protein and nucleic acid quantification, enzyme activity, enzymemechanism and thermal denaturation; thenedit the method parameters to meet yourunique requirements. VISIONcollectsoftware provides calculations forMichaelis-Menton Km and Vmax parameters using methods suchas Lineweaver-Burke, Hanes-Woolf and Eadie-Hofstee.

Leverage the superior performance of Peltiertemperature control for accurate and reliablemeasurements. Whether you are performingkinetics experiments or thermal denaturationexperiments, we have a temperature controlaccessory for you. Choose from a PeltierSingle Cell Holder or a Peltier 8-PositionCell Changer for temperature control andsample temperature monitoring from -10 to100 °C. The Automated Referencing

Stage, Thermostatted 8-PositionCell Changer and Thermostatted

Single Cell Holder offer temperature control using a liquid recirculation.

Visualizing the entire absorption spectrumat each time interval takes your kineticsanalysis to the next level. Eliminate driftfrom long-term experiments using theAutomated ReferencingStage which alternates quickly between measuring your sample and a control.

Use the Ultra Kinetic mode to acquire afull spectrum every 20 milliseconds, givingyou access to a complete set of data at anastounding 50 data point per second collectionrate. Our rapid mixing accessory provides complete assurance for accurate kineticsdata with an inte-grated triggeringmechanism.

Fast data acquisition and a rapid mixing accessory combine for full spectrum of your reaction every 20 milliseconds.

Analyze and presentyour data effectivelywith 3D graphicaldisplays and fullzoom and rotationcapabilities.

Peltier Single Cell Holder

Equation Expression provides a practical tool that allowsautomation of routine calculationsfor methods such as chlorophylland ibuprofen.

View a calibration curve foryour standards at any wave-length by sliding the cursoracross the spectrum. Curvedata and statistics are automatically displayed atthe bottom of the screen.

Accessories allow you to customize your EvolutionArray for the unique demands of your laboratory.Our extensive line of accessories complements yourwork and allows you to get the most accuracy andproductivity from your UV-Visible spectrophotometer.Easy software interaction and unique sampling features make these accessories the right fit foryour laboratory.

ACCESSORIES FOR ALLYOUR SAMPLING NEEDS

TRANSMITTANCE SPECULAR REFLECTANCE DIFFUSE REFLECTANCETransmission measurements on opticalflats, films, glass and plastic samples ofall shapes and sizes are simple with thesophisticated and precise mounting optionsof the Advanced Transmittance Holder. • A spring-loaded clamp securely holds

objects up to 6 cm thick.• A magnetic plate precisely positions

films and thin samples.• A 10 position variable height stage

allows you to:– support samples of different sizes– place specific regions of a sample in

the beam

If films are the only solids you need tomeasure, choose the cost-effectiveMagnetic Film Holder. Supporting both10 mm pathlength cuvettes and film samplesin a single accessory, the Magnetic FilmHolder protects the surface of your filmsample between two rubberized magneticplates. Using two plates allows you to precisely position your film while the sampleis outside the sample compartment.

Reflective samples and surface films areeasy to measure with the Evolution Array’supward looking reflectance accessories.An upward looking geometry allows you tomount your sample by laying it over thereflectance port.

The 34° Specular ReflectanceAccessory is ideal for studying surfacefilms using the thickness mode ofVISIONcollect software. Each SpecularReflectance Accessory ships with a standardmirror mounted in an aluminum case.

The EV Array Diffuse ReflectanceAccessory is a unique system poweredby its own dedicated 150 Watt xenon lampproviding intense sample illumination. Thissystem provides greater sensitivity allowingyou to measure reflectance differences ofless than 1%. The accessory is CIE compliantfor measuring L*a*b*, Gardner and APHAcolor with the Evolution Array Color software.

Powerful graphing, measurement anddata analysis functions help you measureeven differences between samples.

Accessories For Every Application

The Evolution Array spectrophotometer with transmittance and reflectanceaccessories brings speed and convenience to materials measurements.

S A M P L E W I T H P R E C I S I O N A N D F U L L C O N F I D E N C E

VISIONcollect includes a Thickness module which automatically calculates the thickness of a film on a surface based on the number ofinterference fringes in a specified wavelength range. This calculationcan be performed for films on reflecting substrates and for films depositedon transmitting substrates such as glass or plastic.

Materials Analysis Software

Thermostatted Single Cell Holder

Variable Pathlength Cell Holder

Thermostatted 8-Position Cell Changer

AdvancedTransmittance Holder

Magnetic Film Holder

34° Specular Reflectance Accessory

Optional Evolution Array Color software calculates L*a*b* color and colordifference between samples as ΔE and individually as L*, a* and b*.

Chromaticity values x and y are plotted automatically on a chromaticity diagram anddeltas for yellowness can be displayed. Othercolor standards and measurements are also supported, including:

• APHA• Gardner• ASTM E313• ASTM D1925• Hunter Lab

Rapid Mixing Accessory

Peltier Single Cell Holder

and 8-Cell Changer (not pictured)

Automated Referencing Stage

Automated and ManualSipper Systems

EV Array Diffuse Reflectance Accessory

DRAFT COPY

BR51851_E_01/10M

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www.thermo.com

Our Pledge of SupportA Fundamental UV Spectrophotometer RequirementYour instrument is backed by a highly trained service and applications

support team dedicated to improving your productivity, reducing

your total cost of ownership and ensuring compliance across your

laboratory. Available services include:

Installation ReadinessPrior to installation, we’ll work with you to develop an implementation plan to determine the best way to integrate your Thermo Scientific instrument and any third-party components with your current lab configuration. In regulated environments,our certified engineers will perform detailed Installation and Operational Qualificationprocedures to verify that your equipment is not only installed according to factory specifications but complies with regulatory guidelines.

Maintenance Peace-of-MindFrom depot repair to critical on-site support, we offer repair services that include 24/7options to help meet your application’s uptime requirements. In addition, whether it’spreventive or corrective maintenance, applications assistance or qualification, a support plan assures top performance of your instrument while eliminating the uncontrollable costs of unplanned maintenance and repairs.

Technical ExpertiseTo assure you work smarter in the lab, we offer a breadth of training and tech supportservices at your fingertips. Your training options may include sample preparation,method development and instrument maintenance – all customized to your lab. In addition, our Tech Support staff will provide solutions to your tough questions rangingfrom troubleshooting to technical and operational assistance.

Find out more by visiting www.thermo.com/lifecycle.