The use of Solea spp. as sentinel species in Europe · (2016). The use of juvenile Solea solea as...

The use of Solea spp. as sentinel species in Europe

Montserrat Sol

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Solea solea

S. senegalensis : subtropical S. solea: temperate

Comercial species Not distinguished in market

Solea senegalensis

Not filled: bioaccumulation Filled: Bioaccumulation and biomarkers

Solea senegalensis

Solea solea

Sole

a sp

p.

Plasma Immune system Sex hormones VTG

Liver Histology Phase I & II enzymes Antioxidant enzymes and LP

Gonad Histology Esterases

Muscle & Brain AChE LP

Gills Phase I & II enzymes Antioxidant

enzymes and LP

Kidney Metallothionein

Bile PAH, NP metabolites

Cytosol

Microsomes

Liver (microsomes and cytosol) Other tissues (S10)

7-ethoxicumarin-O- deetilasa (ECOD) -CYP2A6 (Pharmaceuticals)

7-ethoxiresorufin-O-deetilasa (EROD) -CYP1A1 (xenobiotics)

7-methoxiresorufin-O- deetilasa (MROD) -CYP1A2 (less abundant in fish)

7-benziloxi-4-trifluorometilcumarin-O-debenziloxilasa (BFCOD) -CYP3A4 (endogenous; e.g. Sex hormones)

Glutathione peroxidase (GPX)

UDP-glucuronosyltransferasa (UDPGT)

Glutathione-S-transferasa (GST) Glutathione reductase (GR)

Catalase (CAT)

CYP 450

CbEs phase II Antioxidant

aNA (=235) pNPA (=405)

PHASE I

AChE CbE

Brain more adequate tissue -NA better substrate for OP pesticides

EROD CbE GST

microsomes Citosol microsomes citosol

Vmax S. solea 97.09 72.99 181.82 256.41

S. senegalensis 24.88 35.71 212.77 303.03

Km S. solea 2.69 0.23 0.07 0.67

S. senegalensis 1.11 0.10 0.09 0.61

Vmax/Km S. solea 36.10 322.58 2500 384.62

S. senegalensis 22.32 344.83 2500 500.00

Phase I Phase II

BTI index = Phase I S. senegalensis < S. solea Phase II

Phase I (EROD) reactive oxygen species Phase II (GST) strictly detox. pathway

0

200

400

600

800

1000

1200

ng/g

d.w

PAHs

02468

1012141618

CostaBrava

Maresme Garraf Creixell Tarragona Cambrils Delta delEbre

ng/g

d.w

PCBs

0

50

100

150

200

250

ng/g

d.w

DDTs

0102030405060708090

CostaBrava

Maresme Garraf Creixell Tarragona Cambrils Delta delEbre

ng/g

d.w

.

PBDEs

POPs (GC-MS) and PPCPs (LC-MS)

Species Metallothionein Selenium

Cd Cu Hg Zn Cd Ag Hg

S. solea (n=48) r p

0.336 0.019

0.483 0.001

0.464 0.001

-0.247 0.088

0.002 0.991

-0.002 0.991

0.036 0.810

S.senegalensis (n=21) r p

0.583 0.004

0.452 0.035

0.496 0.019

0.087 0.700

0.713 0.000

0.104 0.644

0.600 0.003

Se protection role in front of Hg

*

*

0.1

1.0

10.0

100.0

CRL (14) KETO (5) GEM (7) NP (5) EE2 (5)

mg/

ml

a) VTG *

0.0

0.5

1.0

1.5

2.0

CRL (14) KETO (5) GEM (7) NP (5) EE2 (5)

ng/m

l

b) Progestin-17,20-P

NP endocrine disruptor: VTG and Progestin (MIS and pheromone)

O-deethylase activity (susbstrate) Metabolite Ex/Em (nm) Substrate

(M)a Mammalian CYPsb

EROD (ER;7-ethoxyresorufin )

Resorufin sodium salt 537/583

2 1A1 > 1A2, 1B1

PROD (PR; 7-pentoxyresorufin) 5 2B

BROD (BR; 7-benzyloxyresorufin) 2 1A, 2B, 3A

MROD (MR; 7-methoxyresorufin) 5 1A2

CECOD (CEC; 3-cyano-7-ethoxycoumarin) 3-cyano-7-hydroxy-4-methylcoumarin 408/455 10 1A2, 2C9, 2C19

BFCOD (BFC; 7-benzyloxy-4-trifluoromethylcoumarin)

7-OH-4-trifluoromethylcoumarin 410/538 200 3A4, 2C19

ECOD (EC; 7-ethoxycoumarin) 7-OH-coumarin (umbeliferona) 370/450 100 2A, 2B, 1A

EROD MROD CECOD BROD PROD ECOD BFCOD

Control (14) 14.5 1.2 4.6 0.5 14.2 1.9 1.6 0.2 0.6 0.1 5.4 0.6 49.3 8.4

NF (5) 169.0 46.1* 51.2 22.4* 134.6 31.3* 3.8 1.6* 2.1 0.7* 39.1 8.6* 150.8 28.5*

KETO (5) 10.4 1.4* 3.6 0.3 8.7 1.8 0.9 0.1* 0.2 0.1* 3.7 1.4 33.4 8.5

GEM (7) 13.5 1.1 6.4 0.5* 24.0 3.6* 2.2 0.3 0.8 0.3 24.3 2.9* 39.9 6.7

NP (5) 11.4 2.6 3.5 0.8 11.1 2.9 1.7 0.3 0.5 0.1 12.8 3.6 56.9 12.0

EE2 (5) 17.9 3.7 5.9 1.4 14.0 4.5 2.7 0.6* 1.0 0.2* 12.5 2.3* 253.4 74.6*

Adaptation to lab Acclimatation to temperature

15 C 20 C

Observed for EROD and membrane bound enzymes to compensate for lower reaction rate at lower temperatures.

* *

*

**

* 00

10

20

30

40

50

60

70

80

EROD BFCOD CbE (pNPA) UDPGT

15C

carrier

IBU

CBZ

* * 0

10

20

30

40

50

60

70

80

EROD BFCOD CbE (pNPA) UDPGT

20C

Temperature influenced Xenobiotic metabolism.

Drugs afected plasmatic parameters including osmoregulation.

EROD (CYP1A1) BFCOD (CYP3A4) CbE (lipid regulators)

Grfico1

92.409856449480.110414006196.546064880912.61573941591.4148313859

83.575414041461.932095703597.65655257094.37426709862.2903345634

87.68358720468.12873942490.40107710024.06058935672.0770491536

84.400042576361.01893444292.85428828884.66494622682.951084999

81.83666315562.678863765163.29299698184.14494496293.442536586

98.789257192681.154904635153.32854148932.98483066482.9445490782

87.919559130877.422512529392.8593653795.04799118552.6856288437

80.669682791779.339293236991.96191939971.93041110232.9359586396

82.240116686383.679089849799.75454364660.65979187291.4801236611

83.578826741695.068975585498.12169011271.84318918851.7280918776

19.19382207365210.07157953484.86565026491.7624589837

EROD

BFCOD

CbE (pNPA)

*

*

*

*

*

*

*

*

*

esterases

sense dilsense dilsense dilsense dildil 1/2 except Gill A1 dil1/4nmol/min/mg prot

MuscleAChE (1mM)meanPrChE (1 mM)meanBuChE (1 mM)meanCbE(pNPA; 1mM)meanCbE(aNA; 0.25mM)meanproteinaAChEPrChEBuChECbE (pNPA)CbE (aNA)MuscleAChEPrChEBuChECbE (pNPA)CbE (aNA)AChEPrChEBuChECbE (aNA)CbE (pNPA)

2013A10.0775170.0747940.0707110.0740.0442870.0425390.0414320.0430.0336050.0328410.0319590.0330.0310650.0275520.0264640.0280.0302160.0242320.0200880.0258.317.44.33.32.12.920138.94.93.32.03.220138.89400745854.885438893.28559155363.19760061511.9644636412

A20.0848520.0822630.0796830.0820.0448970.0439770.0465810.0450.0315480.0327020.0348750.0330.029050.0273160.0261450.0280.0287650.0301440.0280910.0299.387.34.02.91.83.020148.24.43.11.93.020148.19845807624.416863593.05952163043.01113430671.8901665777

A30.0835950.0756310.0811790.0800.0431720.0382120.0453480.0420.0289820.0280240.0275050.0280.0352680.0340680.0340120.0340.0232650.0242620.0235220.0249.397.13.72.52.32.4201510.76.34.51.34.2201510.70099191916.34332896834.5090804384.18300314941.3165979453

A40.0872860.0824960.0789420.0830.0716290.068890.0716080.0710.0509450.0515940.0494440.0510.0371650.0333770.0334320.0350.0444860.0455580.0434190.04410.926.35.43.82.03.9SEM

A50.126990.11910.12070.1220.0448640.0416160.0449390.0440.0290690.0251040.0278730.0270.0307110.0282290.0293120.0290.0243310.0226590.0221350.0239.5010.73.82.41.92.320130.63906683040.38809561720.30985222090.27411842410.0586047253

A60.106910.0995690.103740.1030.057030.0519760.0516850.0540.0372870.0361190.0321470.0350.0350010.032090.0334470.0340.0309390.0305240.0304250.03111.757.33.82.51.82.520140.70336368170.33575844210.25730573920.31719101770.0974984161

A70.127210.124370.129380.1270.0677160.0611670.0599890.0630.0441250.0384180.0405440.0410.0335310.0302730.0292420.0310.0367670.0367320.0355180.0369.7710.85.33.52.03.620150.55679457790.29701303270.26167787030.24371149860.0419469459

A80.151260.152380.149980.1510.0948450.096290.0958280.0960.0750740.0704920.0719220.0720.0367610.0371010.0360790.0370.0550640.0586630.0566110.05710.5811.87.55.72.25.2

A90.171850.129070.17220.1580.0858690.0948970.0888410.0900.0517310.0571530.0503310.0530.034480.0347010.0367180.0350.0442130.0443850.0433640.04412.2510.76.13.61.83.5

A100.170820.123080.13010.1410.0609890.0761680.0825380.0730.0429270.0372290.0398410.0400.0338660.0333210.0321870.0330.0342950.0335280.0348460.03411.959.85.12.81.72.8

2014B10.12020.123450.118120.1210.0692170.0698050.0688240.0690.0439520.0460230.0444440.0450.0354040.0326330.0352770.0340.0372930.0360070.0381710.03713.487.44.32.71.62.6

B20.108060.108290.109630.1090.0640350.0633190.0411310.0560.0480290.0465370.0473240.0470.0393260.0378560.0371390.0380.0380580.0370820.0379980.03811.667.74.03.42.03.1

B30.109190.106180.102790.1060.0603010.0581660.0818510.0670.0452230.0492880.0460870.0470.0358860.0347910.0345470.0350.0407580.0359590.0381990.03810.648.25.23.62.13.5

B40.152530.143170.14480.1470.0799790.0826160.0807920.0810.0591150.0619730.0593550.0600.0392480.0380170.0364770.0380.0515020.047850.0491370.04910.3111.86.54.82.34.6

B50.0899080.0746070.0868930.0840.0483270.0511040.0430920.0480.0308390.0305560.0336280.0320.0332910.0315060.0301460.0320.0250210.0231180.0249450.02412.015.83.32.21.61.9

B60.118240.112240.108610.1130.067610.065120.064470.0660.0436640.0440780.0437760.0440.033930.0348170.0341450.0340.035450.0352190.0364750.0369.789.65.63.72.23.5

B70.0948220.0919580.0866260.0910.0519240.0496880.0465010.0490.0383730.0405960.0395850.0400.0347550.033630.0329740.0340.0317810.0281720.0312040.03013.345.73.12.51.62.2

B80.150340.148260.148480.1490.0857310.0463540.0136740.0490.0330670.0330.0425480.0351130.0344880.0370.0509310.0411550.0537670.04910.3611.93.92.62.34.5

B90.111820.119940.112220.1150.0642250.0646790.0668380.0650.0419880.0387540.0416240.0410.0308720.0348150.0331440.0330.0274780.0281920.0278880.02813.647.04.02.51.52.0

B100.10380.097520.100050.1000.0615470.0668080.0650550.0640.0491180.0438690.0173090.0370.0314140.0314330.0347980.0330.0271880.0255160.0266330.02611.837.04.52.61.72.1

2015C10.0930990.0867450.0937150.0910.0617480.0563180.0558580.0580.0454860.0438120.0424690.0440.0200730.0184070.018290.0190.0321530.0325010.0320560.0327.999.46.04.51.53.9

C20.0831640.0812940.0829590.0820.0539750.0518440.0500360.0520.0390280.0382820.0354550.0380.0154440.0151330.0156650.0150.031360.0320160.0327660.0328.318.25.23.71.23.7

C30.0817580.0835380.0718850.0790.052670.0504160.0483960.0500.0347680.0349230.0333770.0340.0149220.0149090.0138630.0150.0296410.0298820.0281650.0297.388.95.73.81.23.8

C40.0823220.0807690.0860660.0830.0485870.043230.0467790.0460.032780.0306650.0320910.0320.012040.0127160.0130970.0130.0257240.0248220.0240880.0257.129.75.43.71.13.4

C50.103610.101680.0997320.1020.0637840.060040.062080.0620.0431840.0402650.0432850.0420.0164410.0152810.0158480.0160.0361840.0339830.0342650.0357.3211.57.04.81.44.6

C60.116690.106660.112880.1120.0713710.0663310.0666760.0680.0505720.0480550.047940.0490.0166170.0173480.016920.0170.0415560.036330.040.0397.1113.07.95.71.55.3

C70.108070.101710.0953170.1020.0624230.0558570.058520.0590.0417940.0419020.0412560.0420.0150120.0152050.0160440.0150.032810.0332690.0320330.0336.8912.27.15.01.44.6

C80.0864550.0830590.0863260.0850.0542420.0505260.0541160.0530.0374310.0372570.0354910.0370.0154120.0160270.0151140.0160.0291840.0280240.0283010.0297.109.96.24.31.43.9

C90.116140.12080.1230.1200.0682870.0643150.0676920.0670.0511410.0532190.0557410.0530.0163710.0164680.0160.0433380.0410070.0437670.0437.4813.37.45.91.45.5

C100.0973010.101890.115110.1050.0548220.0536470.0551020.0550.03510.0300980.0389310.0350.0147830.0152530.0158950.0150.0276620.0250290.0285080.0277.9410.95.73.61.23.3

B10.109770.112580.110510.1110.0661150.0653010.0676670.0660.0418980.0424110.0349580.0400.0181960.0180420.0192730.019

B20.105770.105060.107830.1060.0644630.0653940.0697930.0670.0511950.0496670.0529620.0510.0191950.0214190.022030.0210.0352720.0357070.0393660.037

B30.101280.103010.109730.1050.0656350.0639450.0697770.0660.0476750.0486940.0521010.0490.0182760.0198210.0191890.0190.0330640.0337360.0522850.040

B40.14360.139470.142560.1420.0853580.0857220.085650.0860.0641470.0633670.0680130.0650.0209050.021

B50.0881960.0891620.0676420.0820.0521670.0535260.0563620.0540.0332320.0300050.0253010.0300.0182840.0167090.0191730.018

B60.123760.11770.126230.1230.0693930.0693470.0778220.0720.0496370.0410540.0449290.0450.0187520.0199260.0195950.0190.0329180.0330930.0311890.032

B70.0288480.0281020.0324690.030

B80.0402420.0414690.0529880.045Gills

B90.0293260.0152210.0324610.026

B10AChEPrChEBuChECbE (aNA)CbE (pNPA)

GillsAChE (1mM)meanPrChE (1 mM)meanBuChE (1 mM)meanCbE(pNPA; 1mM)meanCbE(aNA; 0.25mM)meanproteinaAChEPrChEBuChECbE (pNPA)CbE (aNA)201313.96.74.87.610.0

2013A1sense dilmeansense dilmeansense dilmeansense dilmeandil 1/2 except Gill A1 dil1/4mean20148.64.93.36.810.1

A20.173150.170840.176940.1740.106470.105360.10760.1060.0805870.0810670.0803610.0810.147670.136470.130510.1380.0484980.0458390.0441660.0469.4615.29.37.19.19.4201511.36.24.76.85.0

A30.133540.133440.137350.1350.0814970.0839220.0831040.0830.0635940.0612790.060810.0620.110770.112240.115820.1130.0556470.0495930.0525110.0537.7114.58.96.69.26.6SEM

A40.0528240.0533430.0539030.0530.0287240.0284910.0305540.0290.0122920.0122930.0114530.0120.11980.0818550.0839240.0950.0328650.0335580.0314470.0336.337.03.81.69.45.020131.41875051270.70974468980.59431564360.49201434330.4315440125

A50.133820.0990840.0971350.1100.053850.0557770.0491760.0530.0432730.041790.0405790.0420.119930.112580.113590.1150.0424910.0413090.043930.0436.8613.36.45.110.56.020140.86162386180.57234096640.45507622710.36851095080.3006005013

A60.0751230.0733730.0708040.0730.0424620.0410970.0430290.0420.0316060.0306670.0300520.0310.101650.0931790.0615720.0850.0518090.0440120.0488620.0485.7810.56.04.49.28.020151.06114502560.56808477960.47554131110.45364956580.7937400483

A70.0915350.0920540.0901590.0910.0101050.00998790.0113520.0100.00851760.00779880.00811640.0080.0472320.0454010.047650.0470.020040.025360.0219840.0223.2423.32.72.19.06.7

A80.142070.142050.133990.1390.0693580.0738250.0712610.0710.0479610.0467430.0476420.0470.0934390.109220.102280.1020.0645580.0623180.0643040.0647.0416.48.45.69.08.7

A90.0878390.085380.0849460.0860.053760.0549270.0544240.0540.0415490.0407650.0442190.0420.123140.123770.130270.1260.0534970.0589280.0562480.0566.0411.87.45.813.08.9

A100.0977850.0753310.0789520.0840.0467180.0429930.0426570.0440.0329710.0309720.0320820.0320.0865410.0930520.093450.0910.0481090.0450630.0464590.0475.1113.67.15.211.18.8

2014B10.0573530.0521050.0517940.0540.0273420.0269550.0249890.0260.015910.0142350.0161270.0150.0962530.0863430.0954740.0930.0426820.041630.0415310.0425.228.54.22.411.17.7

B20.0520230.0519060.0527950.0520.0298880.0312470.0320170.0310.0188630.0198640.0196480.0190.104960.1090.108890.1080.0522760.0501110.0552640.0537.485.83.42.29.06.8AChEPrChEBuChECbE(pNPA)CbE(aNA)

B30.0974880.0958690.0960740.0960.0604170.0600520.0576070.0590.0436960.0426270.0431730.0430.137130.127840.130650.1320.051690.0538970.0533650.0539.078.85.43.99.15.6gills11.35.94.38.37.1

B40.0971750.0943270.0984360.0970.0584750.0548510.0586620.0570.0406280.040250.0406790.0410.104210.0984220.103670.1020.0549620.051010.0527940.0536.3812.57.45.310.08.0muscul9.35.23.61.73.5

B50.0642660.0658540.0651460.0650.0343650.0346980.035710.0350.021040.0203240.0202110.0210.114760.115090.11160.1140.0386450.036290.0404010.0386.348.54.62.711.25.8citosoldiferents102.744065592679.7736442775

B60.0696330.0706650.0678310.0690.0397720.0391330.0405260.0400.0269490.0265420.0262960.0270.116420.108940.114530.1130.0565020.0537670.0560380.0557.387.84.53.09.67.2microsomes138.380750740875.865143992

B70.0514030.0513820.0515160.0510.0250590.0275660.0225480.0250.0186910.0181120.0172750.0180.125310.124070.126750.1250.0364050.0399130.0429590.0407.695.52.71.910.25.0

B80.0441070.043240.0422230.0430.0267030.0249480.0254630.0260.0146080.0142440.0155450.0150.0762250.0725590.0717380.0740.0319210.0235640.0300930.0294.398.14.82.810.56.3

B90.0958630.0968260.101380.0980.0580210.0597670.0603280.0590.0441880.0428110.0445930.0440.109610.105450.103410.1060.0525260.0516980.0548220.0535.8213.98.46.211.48.8

B100.0598610.0687560.065470.0650.0325520.0352810.0323840.0330.0235310.0222690.0208010.0220.120110.108780.125530.1180.0600620.0581590.0597660.0598.346.43.32.28.96.8

2015C10.0617180.0622520.0588150.0610.0418060.0413180.0410420.0410.0309160.0305890.0284730.0300.128220.125460.123710.1260.0463310.0460670.0446570.0466.787.45.13.711.66.5

C20.072710.0759960.0754340.0750.0404670.0372760.0401450.0390.03140.029950.0264650.0290.0404320.0412780.0371380.0400.0463270.0466820.0459580.0466.938.94.73.53.66.4

C30.13350.142870.139790.1390.0732350.0613810.0664790.0670.0540470.0518540.0504070.0520.0537390.0523270.0478560.0510.0622590.0580110.0585660.0607.3015.77.65.94.47.8

C40.103450.102220.100920.1020.0532850.05080.0507990.0520.0373970.0351810.0338330.0350.0367070.0356610.0357380.0360.0496470.0458390.044790.0478.549.95.03.42.65.3

C50.108260.11650.106870.1110.0663450.0645530.0614920.0640.0473660.0456810.0463140.0460.0509140.0499790.0495020.0500.0569410.0523220.0539670.0547.1212.87.55.44.47.3

C60.126610.124470.130060.1270.0732590.0677430.0687770.0700.0551020.0506460.0501880.0520.0495620.047840.0471670.0480.051930.0513950.0515360.0527.1514.78.16.04.26.9

C70.111470.116370.117640.1150.0624290.0587530.0478870.0560.0495520.0522450.0485610.0500.0562230.0545440.051690.0540.0660680.0679130.06640.0676.7914.06.96.15.09.5

C80.114180.0749190.113060.1010.0642930.0635580.061320.0630.0499910.0499710.0466380.0490.0558550.0543420.0568450.0560.0507050.0453350.04460.0475.8514.28.96.95.97.7

C90.0731280.0704640.0771350.0740.0376850.0330080.0343330.0350.0250.0248060.0244160.0250.0440640.0413640.0415440.0420.0360670.0376760.0357750.0378.307.33.52.53.24.2

C100.0708290.0707130.0715780.0710.0442240.0449890.0471940.0450.035250.0343270.0382120.0360.0585220.0603050.0623270.0600.0518060.0530.0546050.0537.717.64.93.94.96.6

B10.0831830.0931070.0866980.0880.0455370.0462740.0481280.0470.0338830.0372860.0227260.0310.0439240.0456410.0452170.0450.040830.0394680.0422550.041

B2

B3

B40.0809520.0811410.0789720.0800.043390.0450610.0494610.0460.0332020.0336630.0339210.0340.0367350.0337520.0304210.0340.048470.0470350.0525760.049

B5

B60.0692010.0725270.0673590.0700.0385660.0398920.038950.0390.0280130.0258250.0282020.0270.0407560.0411280.0433920.0420.0478170.048090.0521930.049

B70.0393780.0386810.0406350.0400.0211930.020650.0205080.0210.0141240.0140250.0134280.0140.045290.0452120.0507720.0470.0287590.0278850.0299070.029

B80.0365330.0370410.0366580.0370.0239750.0239880.0229880.0240.0128440.0134190.0151110.0140.0291220.0274250.0282070.0280.0256630.026880.0262930.026

B90.0929360.107610.0936050.0980.0572950.0545770.0573880.0560.040810.0440450.0442940.0430.0403750.0417750.0429180.0420.0473960.049070.0476560.048

B100.0601260.0592790.0600850.0600.0286950.0325250.0320950.0310.0166850.0168310.0164460.0170.0480510.050470.051640.0500.0527490.0576350.0559540.055

esterases

0.63906683040.70336368170.5567945779

0.38809561720.33575844210.2970130327

0.30985222090.25730573920.2616778703

0.27411842410.31719101770.2437114986

0.05860472530.09749841610.0419469459

2013

2014

2015

nmol/min/mg de protena

Musculatura

ab

a

a

a

a

a

a

a

a

a

b

b

b

b

b

CS&LDH

MuscleCS (dil 1/20)meanprotActiv

2013A10.0125940.00682810.0114460.01028936678.3120.5MuscleCSCS

A20.0145790.0139010.0142190.0142339.3825.1201329.0Brnquies201392.18513169134.4595112873

A30.0138610.013930.0134180.01373633339.3924.2201434.7201481.80311147173.876420709

A40.0254710.0269750.0262650.02623710.9239.8201530.439.50*1.3201597.71926065293.3854109933

A50.0160380.0157550.0160280.01594033339.5027.8t-test1.6679331832Mscul201329.04625914041.6679331832

A60.0219850.0204690.0212610.021238333311.7529.90.0602.2696009412201434.70797322732.2696009412

A70.0188130.0180650.0182370.01837166679.7731.11.3667832783201530.38121638481.3667832783

A80.0206910.0205940.0207160.02066710.5832.327.9

A90.0245950.0203990.0180640.021019333312.2528.4

A100.023370.0228550.0218440.022689666711.9531.4

2014B10.0312280.0312980.0315420.03135613.4838.5

B20.0234340.0243470.02390.023893666711.6633.9

B30.0257120.0263090.0234230.02514810.6439.1

B40.0283950.0281590.0283050.028286333310.3145.41.3

B50.0225530.0210020.0209510.02150212.0129.6

B60.0213230.0201610.0208480.02077733339.7835.1

B70.024750.0233510.0228650.023655333313.3429.3

B80.0254010.0257980.0237580.024985666710.3639.9

B90.0302120.031070.0294790.030253666713.6436.7

B100.0144890.0121310.0153110.01397711.8319.5

2015C10.0164360.0161430.015660.01607966677.9933.3

C20.0141480.0145250.0142240.0142998.3128.5

C30.0115770.0119560.00947210.01100177.3824.7

C40.0119660.0117270.011960.01188433337.1227.6

C50.0169170.018250.0169880.0173857.3239.3

C60.0154110.0142440.0150190.01489133337.1134.6

C70.0129460.0126080.0113080.01228733336.8929.5

C80.0139980.0135840.0127350.0134397.1031.3

C90.0122470.0126690.0124720.01246266677.4827.6

C100.0126820.0134610.0134170.01318666677.9427.5

2013A10.0131570.0125120.0133140.01299433337.4328.9

A20.014610.0146910.015110.01480366677.5132.6

A30.0144040.0153560.0158640.0152087.6033.135.6

A40.0219350.023870.0242370.02334733338.1447.5

A50.0141620.0147240.0151530.01467966676.8335.6

A60.0178180.0168270.0185010.01771533338.1835.8

GillsCS dil (1/30)meanLDH (dil 1/30)meanprotActiv CSActiv LDH

2013A10.027470.0265590.0262970.02678-0.089679-0.096824-0.096354-0.094299.4670.3432.9GillsCSLDH

A20.0311270.0293840.0316910.03073-0.085212-0.082277-0.091088-0.086197.7199.0485.4201392.2623.1

A30.0177730.0168830.0190050.01789-0.078981-0.084379-0.086876-0.083416.3370.1571.6201481.8590.6

A40.0292320.0283490.031620.02973-0.076319-0.079797-0.085047-0.080396.86107.6509.0201597.7551.9

A50.025030.0257730.0259930.02560-0.083893-0.085401-0.088277-0.085865.78109.9644.94.459511287377.6425688888

A60.0109450.0114320.0119840.01145-0.037723-0.035514-0.0405-0.037913.2487.7507.73.876420709104.4903958911

A70.0244950.0229610.0243280.02393-0.099082-0.1051-0.11029Dil 1/60 A7-0.104827.0484.31292.73.385410993326.0695272907

A80.025590.0242770.025520.02513-0.072948-0.074217-0.075371-0.074186.04103.2533.1

A90.0192610.01926-0.072379-0.077357-0.081231-0.076995.1193.5654.0

A100.0202320.02023-0.079747-0.068835-0.067945-0.072185.2296.2600.2

2014B10.0245080.0250520.0224070.02399-0.082187-0.090363-0.079475-0.084017.4879.6487.5

B20.0231150.0230420.0216660.02261-0.087882-0.084659-0.090099-0.087559.0761.8418.8

B30.0217410.0207970.0198780.02081-0.075973-0.075148-0.079294-0.076816.3880.9522.4

B40.0258570.0252770.0223950.02451-0.087527-0.086975-0.090396-0.088306.3495.9604.5

B50.0281940.0241860.0246310.02567-0.071316-0.067558-0.071275-0.070057.3886.4412.3

B60.0223560.0220850.0199070.02145-0.066115-0.062547-0.064929-0.064537.6969.2364.4

B70.0186990.0144790.0175820.01692-0.053934-0.056275-0.056522-0.055584.3995.7549.8

B80.0236490.023080.0225380.02309-0.096107-0.10016-0.10646Dil 1/60 B8-0.100915.8298.41504.3

B90.0244480.02445-0.09047-0.085797-0.086349-0.087548.3472.8455.8

B100.0188630.01886-0.081371-0.07622-0.087874-0.081826.0577.3586.6

2015C10.0262560.0240950.0243690.02491-0.085525-0.087313-0.084709-0.085856.7891.2549.7

C20.027920.0252130.027780.02697-0.088624-0.08372-0.085352-0.085906.9396.6537.9

C30.0247060.0248030.0240710.02453-0.10718-0.10881-0.1091-0.108367.3083.3644.0

C40.0352860.0343590.0357730.03514-0.092382-0.092893-0.088508-0.091268.54102.1464.0

C50.0275630.0279380.0273510.02762-0.095901-0.094871-0.095159-0.095317.1296.3581.2

C60.0289350.0255930.0295020.02801-0.085755-0.087684-0.084247-0.085907.1597.2521.6

C70.031150.0307350.0318110.03123-0.099181-0.098736-0.10062-0.099516.79114.1636.0

C80.027220.0279420.0274240.02753-0.090734-0.091426-0.087808-0.089995.85116.8667.8

C90.0299350.0303330.0301090.03013-0.076195-0.081742-0.076393-0.078118.3090.0408.4

C100.0280270.0275510.0278710.02782-0.08883-0.087462-0.09464-0.090317.7189.5508.5

B10.0250110.0245930.0265950.02540-0.086159-0.092802-0.087725-0.088906.92791.0557.1

B20.0280320.0242520.0274170.02657-0.091286-0.095909-0.098027-0.095078.02182.2514.5

B3

B4

B5

B6

B70.0188490.0187710.0202760.01930-0.051734-0.061376-0.059431-0.057514.335110.5575.9

B80.0270090.0253570.0267960.02639-0.091632-0.10184-0.10357-0.099015.911110.8727.2

B90.0264580.0248850.0284840.02661-0.074819-0.077316-0.089547-0.080565.707115.7612.8

B100.0253810.0181230.0297890.02443-0.093313-0.096148-0.094945-0.094805.961101.7690.4

2013

2014

2015


Brnquies

1.4187505127

0.8616238618

1

0.7097446898

0.5723409664

0.5943156436

0.4550762271

0.4920143433

0.3685109508

0.4315440125

0.3006005013

CS&LDH

4.4595112873

3.876420709

3.3854109933

1.6679331832

2.2696009412

1.3667832783

CS

nmol/min/mg protena

Activitat CS

ab

a

b

bradford

BSA mg/mlgillsmeanmusculomean

00.32429999110.33689999580.33640000220.332533329700.32359999420.34070000050.34169998760.3353333

0.050.43110001090.34119999410.45149999860.40793333450.050.45539999010.4456000030.43599998950.4456667

0.10.42660000920.56669998170.55180001260.51503333450.10.57279998060.53380000590.5533000

0.20.74190002680.74400001760.76340001820.74976668760.20.63330000640.71399998660.69169998170.6796667

0.30.83160001040.86699998380.84939998390.8493333260.30.87180000540.82200002670.8469000

0.41.00689995290.98699998860.95429998640.98273330930.40.82289999720.9807000160.89639997480.9000000

0.51.11660003661.08500003811.04240000251.08133335910.50.99550002811.14800000191.13759994511.0937000

Musclemusculaturameandil 1/20

2013A10.94309997560.99639999870.97390002010.97113333158.31

A21.0132999421.09060001371.03939998151.04776664579.38

A31.01880002021.06060004231.06690001491.04876669259.39

A41.13429999351.16209995751.17739999291.157933314610.92

A51.06190001960.99849998951.10780000691.0560666729.50

A61.16980004311.23979997631.24370002751.217766682311.75

A71.01639997961.10780000691.10259997841.07559998839.77

A81.07350003721.17700004581.1497999431.13343334210.58

A91.21379995351.26639997961.27869999411.252966642412.25

A101.19939994811.26820003991.2276999951.23176666111.95

2014B11.24179995061.3726999761.4097000361.341399987513.48

B21.16129994391.25179994111.22090005871.211333314611.66

B31.09860002991.19410002231.12090003491.137866695710.64

B40.99559998511.16900002961.17929995061.114633321810.31

B51.11779999731.29050004481.30079996591.236366669312.01

B60.97939997911.07700002191.17349994181.07663331439.78

B71.19649994371.40600001811.39010000231.330866654713.34

B80.9675999881.14839994911.23819994931.118066628810.36

B91.3364000321.35239994531.36940002441.352733333913.64

B101.17089998721.24000000951.25829994681.223066647811.83

Gillsgillsmeandil 1/30

2013A10.86669999360.78649997710.88639998440.84653331849.46

A20.73530000450.78339999910.75260001420.75710000597.71

A30.70260000230.71719998120.64069998260.6868333226.33

A40.72509998080.73960000280.67580002550.7135000036.86

A50.66130000350.69379997250.62010002140.65839999915.78

A60.53670001030.53039997820.5185999870.52856665853.24

A70.73760002850.76029998060.67089998720.72293333217.04

A80.71189999580.65539997820.64810001850.67179999756.04

A90.64259999990.62699997430.60299998520.62419998655.11

A100.62239998580.63190001250.63510000710.62980000185.22

2014B10.72310000660.76410001520.74930000310.74550000837.48

B20.7584000230.87749999760.84509998560.82700000219.07

B30.63289999960.71460002660.72030001880.68926668176.38

B40.6759999990.73769998550.64770001170.68713333216.34

B50.7067000270.81120002270.70240002870.74010002617.38

B60.75510001180.78250002860.73059999940.756066687.69

B70.56169998650.64010000230.55989998580.58723332484.39

B80.65689998870.66140002010.66380000110.66070000335.82

B90.80089998250.84299999480.72390002010.78926666588.34

B100.68470001220.70359998940.62919998170.67249999446.05

BSA mg/mlmeanBSA mg/mlmean

00.31529998780.31880000230.33390000460.322666664900.29440000650.36199998860.36620.340866665

0.050.4077999890.39320001010.40049999950.050.40830001240.41440001130.4918999970.4382000069

0.10.5733000040.54589998720.55260002610.55726667250.10.55629998450.56230002640.55930000540.5593000054

0.20.67250001430.71929997210.76209998130.71796665590.20.68389999870.69139999150.68389999870.6863999963

0.30.79460000990.85449999570.85320001840.8341000080.30.92250001430.81800001860.80620002750.8489000201

0.40.86619997021.02180004120.99089998010.95963333050.41.0601999760.87040001150.93639999630.9556666613

0.51.12170004841.12430000311.12329995631.12310000260.50.97399997710.97350001341.08850002291.0120000045

mean 2 dilucions

Citosol fetgemeandil 1/20meandil 1/40

2013A11.28569996361.45640003681.45299994951.398366649913.350.85769999030.86790001390.86280013.8713.61

A21.51979994771.45959997181.57650005821.518633325914.890.91850000620.89039999250.90445015.0814.99

A31.34940004351.19060003761.37209999561.304033358912.140.74919998650.77499997620.76210010.9211.53

A41.3178000451.46519994741.47280001641.418600002913.610.8317000270.82920002940.83045012.9213.26

A51.77799999711.65120005611.64149999621.690233349817.091.07739996911.13909995561.10825021.0519.07

A61.33650004861.14769995211.5679999591.350733319912.740.8557999730.80449998380.83015012.9112.83

A71.10790002351.18480002881.38839995861.22703333711.160.7361000180.73549997810.73580010.1510.65

A81.277600051.30449998381.44480001931.342300017712.630.77869999410.85850000380.81860012.5712.60

A91.13859999181.18889999391.02610003951.11786667519.760.72860002520.82599997520.81610000130.79023311.7410.75

A101.33920001981.24979996681.2307000161.273233334211.750.78829997780.80779999490.81760001180.80456712.1611.95

2014B11.40530002121.5293999911.59309995171.509266654714.770.93910002710.74839997290.93669998650.87473314.2214.49

B21.08150005341.11600005631.27610003951.157866716410.270.77280002830.74269998070.82929998640.78160011.4910.88

B31.29340004921.28810000421.47370004651.351733366612.750.77880001070.78649997710.78079998490.78203311.5012.13

B41.3094999791.39419996741.41700005531.373566667213.030.73830002550.7901999950.73710000510.75520010.7211.88

B51.05859994891.10549998281.28170001511.148599982310.150.75480002160.76169997450.76999998090.76216710.9210.54

B61.29190003871.34409999851.49890005591.37830003113.090.86360001560.90049999950.90119999650.88843314.6213.85

B71.04449999331.10989999771.24489998821.13309999319.950.75489997860.77880001070.80959999560.78110011.4710.71

B81.15450000761.15429997441.1844999791.164433320410.350.82999998330.85740000010.83359998460.84033313.2111.78

B91.32710003851.1496000291.20200002191.226233363211.140.8665999770.89670002460.89130002260.88486714.5112.83

B101.18669998651.53760004041.37660002711.366966684712.950.84030002360.84539997580.83899998660.84156713.2413.10

BSA mg/mlmean

00.37270000580.35890001060.36860001090.33120000360.32769998910.32929998640.3480666677

0.050.38179999590.43489998580.45480000970.42930001020.42419999840.4023999870.4212333312

0.10.5030999780.5601000190.56160002950.51789999010.5385000110.5871999860.5447333356

0.20.65280002360.68940001730.70709997420.66610002521.05209994320.87629997730.7739666601

0.30.84609997270.82010000940.81389999390.785499990.83399999140.8199199915

0.40.98739999530.97180002930.97390002011.05379998681.11800003051.0209800124

0.51.04560005661.08910000321.03719997411.12390005591.07039999961.10590004921.0786833564

microsomes fetgemeandil 1/40

2013A11.11570000651.16910004621.15369999411.146166682220.30

A21.08669996261.12790000441.12300002571.112533330919.40

A31.12699997431.12090003491.13110005861.126333355919.77

A41.26590001581.22880005841.247350037122.98

A51.09580004221.1519000531.1226999761.123466690419.69

A61.18079996111.16499996191.1658999921.170566638320.94

A70.99019998311.01259994511.02579998971.00953330616.67

A81.04209995271.0486999751.03830003741.043033321717.56

A91.05270004271.05569994451.04100000861.049799998617.74

A101.18069994451.15649998191.12999999521.155733307220.55

2014B11.07949995991.02929997441.00240004061.037066658317.40

B21.05250000951.06190001960.9807999731.031733334117.26

B30.97369998691.02030003070.9861999750.993399997616.24

B40.95260000230.98320001360.9490000010.961600005615.40

B51.01139998440.97189998630.95359998940.978966653315.86

B61.07990002631.08190000061.0648000241.075533350318.42

B71.06970000270.98960000281.00909996031.022799988617.02

B80.92040002350.94480001930.91339999440.926200012414.45

B90.89649999140.97269999980.91560000180.928266664314.51

B101.0054999590.97570002080.97140002250.984200000816.00

BSA mg/mlMarina (musculatura)meanMontse (branquias)meanmean (2)

00.31979998950.32749998570.32480001450.32403332990.39050000910.37950000170.37619999050.38206666710.3530499985

0.050.38299998640.43759998680.44679999350.42246665560.44420000910.47589999440.51630002260.47880000870.4506333321

0.10.52719998360.5347999930.53890001770.53363333150.49369999770.5331000090.56419998410.53033333020.5319833308

0.20.64620000120.67960000040.66750001910.66443334020.68699997660.70050001140.76120001080.7162333330.6903333366

0.30.79659998420.81910002230.81470000740.8101333380.85470002890.84670001270.82819998260.84320000810.826666673

0.40.92419999840.97030001880.95099997520.94849999750.98479998110.98869997260.96979999540.98109998310.9647999903

0.51.03050005441.06159996991.05579996111.04929999511.08239996431.0570000411.04079997541.06006666021.0546833277

musculatura (1/20)meandil 1/20

2015C10.92919999360.92110002040.99440002440.94823334617.99

C20.94730001690.98049998280.98439997430.97073332478.31

C30.88929998870.90259999040.92320001130.90503333017.38

C40.87529999020.89679998160.88719999790.88643332327.12

C50.89060002570.91219997410.89889997240.90056665747.32

C60.86669999360.90130001310.88969999550.88590000077.11

C70.83300000430.89099997280.88639998440.87013332056.89

C80.83829998970.90310001370.91460001470.88533333947.10

C90.87749999760.93150001760.9266999960.91190000387.48

C100.9351999760.94040000440.95800000430.94453332827.94

2013A10.88200002910.91339999440.929000020.90813334787.438.31

A20.90039998290.92489999530.91680002210.91403333357.519.38

A30.91320002080.89999997620.94880002740.92066667487.609.39

A40.93660002950.97850000860.96090000870.95866668228.1410.92

A50.84950000050.88599997760.86169999840.86573332556.839.50

A60.92079997061.00109994410.96179997920.9612332988.1811.75

branquies 1/30araabans

2015C10.7091000080.6814000010.71679997440.70243332786.78

C20.6851000190.69660001990.74720001220.70963335046.93

C30.72689998150.69889998440.75590002540.72723333047.30

C40.7800999880.82169997690.75440001490.78539999338.54

C50.6959000230.72640001770.7330999970.71846667937.12

C60.6898000240.73979997630.73000001910.71986667327.15

C70.69580000640.71030002830.70310002570.70306668686.79

C80.62790000440.67570000890.67210000750.65856667365.85

C90.77060002090.79659998420.75580000880.77433333798.30

C100.73960000280.7577999830.7415999770.74633332097.71

2014B10.679000020.73030000930.71890002490.70940001816.937.48

B20.73960000280.73890000580.80460000040.76103333638.029.07

B3

B4

B5

B6

B70.57069998980.56709998850.62360000610.58713332814.344.39

B80.66979998350.66310000420.65149998660.66146665815.915.82

B90.67839998010.67040002350.60670000310.65183333565.718.34

B100.6689000130.68300002810.63959997890.663833345.966.05

araabans

BSA mg/mlmean

00.37549999360.38629999760.3892000020.37529999020.38589999080.37909999490.3818833282

0.050.48980000620.48829999570.50480002160.49149999020.52920001750.53899997470.507100001

0.10.59579998250.66680002210.61760002370.6111999750.60500001910.64050000910.6228166719

0.20.73390001060.78990000490.77340000870.68389999870.71969997880.79739999770.7496999999

0.30.86379998920.91060000660.93760001660.84979999070.89370000360.89259999990.8913500011

0.40.96649998431.01259994511.02439999580.98489999771.06529998781.05560004711.0182166596

0.51.08430004121.19669997691.19869995121.54139995571.16639995571.16410005091.2252666553

citosol dil 1/40mean

2015C10.60019999740.60470002890.60245001324.58

C20.9537000060.9807999730.97630000110.970266660113.91

C30.95509999990.95240002870.95459997650.95403333513.50

C40.95859998461.01999998091.02009999750.999566654414.66

C50.94969999791.01259994510.97630000110.979533314714.15

C60.86739999060.89850002530.87790000440.881266673411.66

C70.81339997050.85390001540.84210002420.8364666710.52

C80.81849998240.90859997270.88489997390.87066664311.39

C91.04700005051.01800000671.04219996931.035733342215.57

C100.96359997990.96240001921.01010000710.97870000214.13abans

B11.12960004811.16719996931.13320004941.143333355618.3014.215

B20.92769998310.95620000360.92089998720.934933324713.0211.490

B31.02180004121.01839995381.06400001051.034733335215.5511.502

B41.02139997481.02339994911.03419995311.026333292315.3410.717

B50.85659998660.87879997490.89920002220.878199994611.5810.921

B61.06319999691.06050002571.04540002351.056366682116.1014.616

microsomes dil 1/40

2015C10.8580999970.84039998050.85900002720.852500001610.93

C21.03929996491.03419995311.00390005111.025799989715.32

C31.0956000091.11489999291.12080001831.110433340117.47

C41.12080001831.1196999551.11740005021.119300007817.69

C51.12220001221.15919995311.1550999881.145499984418.36

C61.07739996911.08000004291.09210002421.083166678716.78

C71.06340003011.08529996871.09459996221.08109998716.73

C81.14040005211.14719998841.1527999641.146800001518.39

C91.15090000631.12209999561.07140004631.114800016117.58

C101.05710005761.03960001471.00759994981.03476667415.55

bradford

GST&GR&GPX&LDH_S9&Citosol

GillsS9 GST (dil 1/5)meanS9 GR (20 ul dil 1/5)meanprotActiv GSTActiv GRS9 GST (dil 1/5)meanActiv GST

2013A10.0395930.0415880.0417940.0410-0.016397-0.017664-0.017702-0.01739.4625.424.050.0670630.0647720.0663380.066140.9

A20.0370050.0343350.0366840.0360-0.0085434-0.010028-0.010931-0.00987.7127.416.810.0700060.071250.0668650.069452.7

A30.0249150.024750.0254510.0250-0.004164-0.0032641-0.0020124-0.00316.3323.26.550.0422880.0337910.0370220.037734.9

A40.038860.0376650.0381020.0382-0.012731-0.014716-0.022913-0.01686.8632.732.270.0641870.0675330.0627690.064855.4GSTGRGR

A50.0342120.0328930.0337830.0336-0.014156-0.012493-0.01393-0.01355.7834.130.850.0550630.0513380.0481730.051552.2GillGSTGRBrnquies201352.855127563933.5413632871Brnquies201333.5413632871

A60.0189310.0191620.018750.0189-0.013424-0.01331-0.012289-0.01303.2434.352.880.0272470.0274860.0270250.027349.3201352.933.5201455.105488547240.3687134466201440.3687134466

A70.047580.0411920.0435660.0441-0.013166-0.01658-0.016192-0.01537.0436.728.660.0727750.0654890.0692230.069257.6201455.140.4201560.411042521374.2132684912201574.2132684912

A80.0603460.0592420.0592580.0596-0.01571-0.01485-0.018237-0.01636.0457.835.480.092440.0878320.0882360.089586.8201560.474.2Fetge (citosol)2013360.2650102558Fetge (citosol)201345.2857710733

A90.0255910.0237450.0237530.0244-0.024203-0.017932-0.020682-0.02095.1127.953.990.0411680.0446540.0371540.041047.0SEM4.34157755185.09975773352014390.8788780515201440.6838370692

A100.0288130.0267120.0282860.0279-0.02192-0.018775-0.023317-0.02135.2231.453.870.0466240.0435250.0480490.046151.73.28378645783.56620878232015488.7871726843201548.8327703171

2014B10.0322420.0306920.0284670.0305-0.011893-0.012812-0.015561-0.01347.4823.923.640.0532420.050390.0514290.051740.54.79181089413.9470366008SEM4.34157755185.0997577335

B20.0379040.0360410.0354560.0365-0.015364-0.020776-0.01874-0.01839.0723.526.570.0711680.0684810.0694370.069745.03.28378645783.5662087823

B30.0373520.0361980.0356370.0364-0.016941-0.019627-0.018885-0.01856.3833.438.170.0703430.0674260.0682090.068763.04.79181089413.9470366008

B40.0350270.0352090.0327280.0343-0.022904-0.020365-0.02362-0.02236.3431.746.340.0665270.0638740.0654890.065360.331.717244686cito2.2633155309

B50.0304530.0302640.0290850.0299-0.018186-0.024752-0.020609-0.02127.3823.837.850.0513630.0497640.0538680.051741.035.19636377123.6447633459

B60.0394620.0402530.0386370.0395-0.021017-0.019242-0.017097-0.01917.6930.132.770.0905520.0899590.0923160.090969.325.40632682143.606493555

B70.0218570.0220220.0201840.0214-0.018816-0.020423-0.016942-0.01874.3928.556.240.0471510.0491040.0502750.048865.2

B80.0287720.0278410.0242970.0270-0.023391-0.022605-0.017612-0.02125.8227.147.980.058310.0581460.0576020.058058.4

B90.036280.032580.0359670.0349-0.022088-0.020703-0.029007-0.02398.3424.637.830.0669640.0695360.0731870.069949.1

B100.0330580.031360.033420.0326-0.025645-0.024002-0.027941-0.02596.0531.656.290.0577990.0598020.0655960.061159.1

2015C10.058870.0540630.0540020.0556-0.03663-0.035162-0.033283-0.03506.7848.168.09

C20.102710.099760.104230.1022-0.05043-0.048475-0.053374-0.05086.9386.496.50

C30.0528080.04890.0496350.0504-0.03433-0.030716-0.031135-0.03217.3040.557.84

C40.0867960.0872220.0891850.0877-0.044314-0.036104-0.042868-0.04118.5460.263.43

C50.0825350.0834940.0848090.0836-0.042028-0.038309-0.039093-0.03987.1268.873.69

C60.0814450.0775730.0806820.0799-0.040269-0.043493-0.039527-0.04117.1565.575.76

C70.0818480.083250.0787040.0813-0.041399-0.039815-0.037058-0.03946.7970.176.49

C80.0735910.0700720.07410.0726-0.03974-0.035005-0.039943-0.03825.8572.786.13

C90.0736970.0745420.0732880.0738-0.060599-0.049038-0.050735-0.05358.3052.184.84

C100.0537720.0499570.0528860.0522-0.034042-0.034276-0.035863-0.03477.7139.759.36

B10.065580.0638090.0641810.0645-0.02756-0.02358-0.026025-0.0257

B20.0754960.0750190.0751030.0752-0.038828-0.033257-0.037048-0.0364

B3

B4

B5

B6

B70.0366030.0352660.0364240.0361-0.026297-0.0238-0.025116-0.0251

B80.0670080.0629780.0629850.0643-0.027809-0.035264-0.031438-0.0315

B90.0713230.0660740.0708460.0694-0.036578-0.036527-0.031012-0.0347

B100.0638180.0634310.0625310.0633-0.042692-0.043246-0.035384-0.040430.437.054.0Activitats

Livercitosol GST (1/40)meanCAT (dil 1/80)10 ulmeanGR (10 ul sin dil)meanGPX (5 ul sin dil excepte A2 3 ul)meanLDH (dil 1/10)meanProt citosolGSTCATGRGPXLDHcat

2013A10.126680.118410.118480.1212-0.10923-0.11714-0.13531-0.1206-0.091714-0.095654-0.10188-0.0964-0.13643-0.1443-0.14262-0.1411-0.040261-0.041151-0.041745-0.041113.6417.40.5837.3594.1543.60.5813869728581.3869727786

A20.111490.107790.104220.1078-0.096905-0.11249-0.13672-0.1154-0.12621-0.12025-0.1242-0.1236-0.12608-0.14229-0.14418-0.1375-0.047201-0.045643-0.043706-0.045515.0337.20.5143.45138.8343.90.5051382275505.1382274682

A30.0848240.0856150.079780.0834-0.093037-0.11281-0.1066-0.1041-0.076731-0.087832-0.10524-0.0899-0.15063-0.14868-0.16004-0.1531-0.034599-0.040137-0.037253-0.037311.5339.10.5941.11120.5646.80.5927604043592.7604043059

A40.0894560.0854090.0905270.0885-0.10428-0.11438-0.13582-0.1182-0.10245-0.11153-0.11122-0.1084-0.14556-0.15513-0.15152-0.1507-0.026604-0.02672-0.025525-0.026313.3312.60.5843.08103.1728.70.584578345584.5783449896

A50.117960.116450.120820.1184-0.056126-0.070196-0.056729-0.0610-0.15349-0.16453-0.16418-0.1607-0.11672-0.12078-0.1239-0.1205-0.056703-0.058937-0.049772-0.055119.1291.10.2144.4357.3541.80.2099734077209.9734077399505.1444152008

A60.116990.11120.11510.1144-0.057079-0.0677670.044519-0.0268-0.12662-0.13053-0.13018-0.1291-0.085698-0.095934-0.099701-0.0938-0.051544-0.049475-0.047868-0.049612.8418.20.1453.0666.3856.00.1370054717137.005471749165.7179958329

A70.0748520.0686620.0712910.0716-0.11394-0.1707-0.125-0.1365-0.068819-0.086085-0.085788-0.0802-0.11864-0.12969-0.1285-0.1256-0.036214-0.036157-0.035687-0.036010.7315.10.8439.70107.0648.90.8412243218841.2243217692

A80.167320.160620.15710.1617-0.098492-0.14918-0.12351-0.1237-0.088525-0.12003-0.086646-0.0984-0.1204-0.1365-0.13705-0.1313-0.035341-0.034549-0.031319-0.033712.6601.40.6441.1694.6038.70.6442916724644.2916724474

A90.0802010.0741020.0743820.0762-0.085791-0.063867-0.12371-0.0911-0.11862-0.129-0.12689-0.1248-0.091393-0.1215-0.11878-0.1106-0.020464-0.0020769-0.024132-0.015610.7332.40.5661.2293.3820.90.5563148932556.314893204

A100.0659420.0572310.0590110.0607-0.069485-0.060417-0.088022-0.0726-0.11193-0.10732-0.10936-0.1095-0.12799-0.12516-0.15395-0.1357-0.024016-0.0016236-0.032201-0.019312.0238.10.4048.30103.0623.3Activitats0.3987704356398.7704355562

2014B10.167730.161050.148890.1592-0.059238-0.14906-0.14366-0.1464-0.11854-0.11115-0.12722-0.1190-0.13505-0.13866-0.10254-0.1254-0.05297-0.05353-0.054173-0.053614.5515.00.6643.2778.5653.5GSTCATGRGPXLDH0.6626955146662.6955145509

B20.101270.104840.101840.1027-0.10474-0.1166-0.13277-0.1180-0.063041-0.070071-0.063239-0.0655-0.10964-0.11889-0.12422-0.1176-0.027429-0.023819-0.025632-0.025610.9442.30.7131.7198.1334.12013360.30.5145.397.939.30.7120181429712.018142879

B30.132770.128030.14290.1346-0.15497-0.14855-0.14744-0.1503-0.10986-0.12202-0.11529-0.1157-0.12814-0.13123-0.12925-0.1295-0.040582-0.038052-0.0375-0.038712.1520.10.8150.3096.9746.22014390.90.6140.7103.440.50.8134029044813.4029043707

B40.0837280.0826650.0812720.0826-0.08478-0.096246-0.10472-0.0952-0.056003-0.052665-0.059563-0.0561-0.15142-0.1608-0.15538-0.1559-0.038876-0.037879-0.035967-0.037611.9325.90.5324.89119.1745.82015488.80.3848.895.830.60.5263736572526.3736572411

B50.0792860.0712090.081560.0774-0.10432-0.11054-0.1441-0.1197-0.073418-0.071357-0.064459-0.0697-0.11733-0.11535-0.12284-0.1185-0.031459-0.030874-0.030931-0.031110.5344.20.7534.90102.1242.7SEM31.7172446860.06571799582.26331553097.46085968563.62127427230.7453195605745.3195604588611.3291626214

B60.0847420.0862660.0902720.0871-0.18429-0.17058-0.16685-0.1739-0.082665-0.090775-0.090536-0.0880-0.12369-0.13454-0.13425-0.1308-0.04139-0.038093-0.041258-0.040213.9294.70.8233.4885.7342.035.19636377120.04944673523.64476334595.01060673443.39310120670.823724301823.724300955649.4467352015

B70.0736650.0744560.0765580.0749-0.062509-0.075459-0.077182-0.0717-0.079302-0.074637-0.078-0.0773-0.11952-0.12876-0.12807-0.1255-0.028764-0.025953-0.028533-0.027810.7327.70.4438.04106.3137.525.40632682140.02257986133.6064935556.3046938392.64041976310.4393115892439.3115891518

B80.142930.145720.141620.1434-0.08352-0.080216-0.097889-0.0872-0.12654-0.14177-0.1772-0.1485-0.13246-0.12498-0.1265-0.1280-0.044489-0.044473-0.041909-0.043611.8570.70.4966.4598.6353.60.4858001345485.8001344898

B90.0674510.0657610.0741680.0691-0.076653-0.067122-0.11504-0.0863-0.10268-0.093742-0.095423-0.0973-0.20543-0.1743-0.18049-0.1867-0.037986-0.0012775-0.042981-0.027412.8252.60.4439.97132.1630.90.4413347794441.334779424

B100.0904940.0876430.0865380.0882-0.057337-0.091936-0.12811-0.0925-0.11315-0.11398-0.099593-0.1089-0.15951-0.15668-0.18475-0.1670-0.022244-0.003-0.024775-0.016713.1315.80.4643.83115.7618.40.4633110427463.3110426925

2015C10.0601570.0588130.0570410.0587-0.030094-0.031965-0.025285-0.0291-0.031146-0.031277-0.030173-0.0309-0.074135-0.081058-0.065481-0.0736-0.0052192-0.0059377-0.0087981-0.00674.58600.10.4235.50145.7321.00.4169267507416.9267506944

C20.0927360.0912770.0962720.0934-0.071846-0.078671-0.074649-0.0751-0.13306-0.13213-0.12986-0.1317-0.15696-0.15299-0.14672-0.1522-0.044127-0.037653-0.036447-0.039413.91314.80.3549.8999.3341.00.3540114113354.0114112965

C30.141150.138120.147460.1422-0.087853-0.089492-0.11182-0.0964-0.10921-0.11436-0.12287-0.1155-0.12344-0.13039-0.13706-0.1303-0.026589-0.025104-0.023086-0.024913.50493.80.4745.0887.6226.70.4684986408468.4986408059

C40.159720.154430.159840.1580-0.062166-0.10208-0.10033-0.0882-0.12448-0.10883-0.1076-0.1136-0.11682-0.13449-0.12967-0.1270-0.025638-0.025238-0.025376-0.025414.66505.30.3940.8778.6625.10.3948777422394.8777422107

C50.151360.142560.165490.1531-0.088129-0.10412-0.097345-0.0965-0.15828-0.15103-0.15117-0.1535-0.13946-0.1187-0.11828-0.1255-0.026452-0.024819-0.022463-0.024614.15507.40.4557.1980.5225.10.4477418141447.7418141269382.0822026003

C60.108790.105160.109030.1077-0.035389-0.065831-0.076793-0.0593-0.087445-0.088723-0.092382-0.0895-0.088071-0.10501-0.10054-0.0979-0.036553-0.033163-0.037064-0.035611.66433.00.3340.4876.2444.20.3340891602334.089160209622.5798612732

C70.107470.106810.102570.1056-0.0369-0.061028-0.083824-0.0606-0.090758-0.085401-0.088755-0.0883-0.11299-0.13211-0.11839-0.1212-0.029584-0.030824-0.027445-0.029310.52470.60.3844.25104.5740.30.377959052377.959051993

C80.145420.140420.145050.1436-0.07595-0.067351-0.070743-0.0713-0.16979-0.14176-0.14694-0.1528-0.10972-0.11692-0.11888-0.1152-0.028502-0.027105-0.022816-0.026111.39591.30.4170.7591.8333.20.411197445411.1974450208

C90.146590.157470.160150.1547-0.10372-0.087444-0.094976-0.0954-0.19058-0.19245-0.17866-0.1872-0.15692-0.16127-0.1747-0.1643-0.028265-0.025549-0.027741-0.027215.57465.70.4063.3795.7825.30.4019037277401.9037276548

C100.150590.145330.161470.1525-0.025115-0.04446-0.068383-0.0460-0.10493-0.11534-0.10894-0.1097-0.14274-0.15518-0.15686-0.1516-0.023447-0.023375-0.02463-0.023814.13505.90.2140.9597.4224.40.213616282213.6162819903

B10.191870.186110.19460.1909-0.11144-0.1973-0.1319-0.1469-0.15238-0.15043-0.10496-0.1359-0.1216-0.12198-0.13053-0.1247-0.026516-0.025927-0.02811-0.0269

B20.128720.130110.132840.1306-0.098419-0.074475-0.095213-0.0894-0.080547-0.086431-0.083975-0.0837-0.10293-0.11908-0.11311-0.1117-0.015823-0.015511-0.015487-0.0156

B30.170520.183740.180490.1783-0.098746-0.10625-0.096164-0.1004-0.14151-0.14828-0.15206-0.1473-0.099931-0.10515-0.10949-0.1049-0.019854-0.019492-0.02023-0.0199

B40.123720.120750.131530.1253-0.027018-0.080242-0.085825-0.0644-0.071918-0.066923-0.07097-0.0699-0.11939-0.13334-0.12745-0.1267-0.023143-0.021692-0.024579-0.0231

B50.115760.122320.113140.1171-0.036705-0.064116-0.086154-0.0623-0.079929-0.086407-0.092349-0.0862-0.11501-0.1074-0.11995-0.1141-0.018097-0.018892-0.020107-0.0190

B60.124590.127630.125930.1261-0.081212-0.10709-0.15131-0.1132-0.14675-0.12011-0.12277-0.1299-0.12869-0.13664-0.12866-0.1313-0.023465-0.022587-0.02422-0.0234

B7GPX-nou CHP-0.073764-0.071093-0.073343

B8-0.13702-0.14922-0.14736

B9-0.15043-0.13435-0.13675

B10-0.14606-0.14839-0.14083

-0.11058-0.12135-0.12362

-0.10287-0.10226-0.11113

-0.1156-0.12734-0.12489

-0.10703-0.11425-0.12523

-0.15892-0.16588-0.15516

-0.15532-0.15575-0.12972

-0.11877-0.10775-0.11374

-0.10133-0.12093-0.093016

-0.097426-0.10892-0.10478

-0.14376-0.14789-0.12236

-0.11022-0.10898-0.10905

-0.12705-0.12826-0.13009

msole:S'ha de repetir perque reactius no estaven be.

msole:repetida. Bona

GST&GR&GPX&LDH_S9&Citosol

4.3415775518

3.2837864578

4.7918108941

31.717244686

35.1963637712

25.4063268214

GST


Activitat GST

a

a

b

CbEs_liver

Repeticio

CbE (aNA)CbE(pNPA)CbE(pNPA)CitosolMicrosomes

Livercitosol 1/40meancitosol dil 1/10citosol dil 1/10meanProt citosolCbE (aNA)CbE(pNPA)CbE (aNA)CbE(pNPA)CbE (aNA)CbE(pNPA)

2013A10.104620.10010.11230.10570.0792330.0828960.0704550.254580.262720.258490.258613.6149.3118.82013103.389.6153.631709400397.9517143151

A20.0793160.0775880.0809990.07930.109350.0740990.0805940.235050.229710.216160.227015.0101.794.62014102.269.9125.345442054271.9540907466

A30.0572670.0593850.0629220.05990.0477220.039990.0362450.154920.141130.140550.145511.599.878.9201576.530.4136.16510076857.6896269141

A40.0339420.0339660.0338180.03390.0601280.0478550.0498610.108920.109620.104360.107613.349.250.7SEM

A50.0621850.050830.0521590.05510.192790.111650.170610.264440.259360.249110.257619.155.584.4201314.14639306917.741882276717.551962293412.7565339379

A60.0543450.0503840.0520590.05230.110160.159820.0649650.184580.1810.182812.878.489.120147.09880807946.199827786510.29480617515.0902511785

A70.101750.0991880.102060.10100.149770.070950.0669640.217910.223690.220810.7182.3129.6201510.34841318393.071213575718.911761467210.2556833086

A80.078240.0801370.0831250.08050.112920.0805410.0882420.186880.194060.190512.6122.894.5

A90.0718720.0761560.0824950.07680.188530.301440.301990.173680.169560.171610.7137.599.8

A100.0362910.0331720.0348170.03480.115980.0450240.0681870.106460.107090.106812.055.955.8

2014B10.0787220.073570.0919270.08140.209470.202070.18270.198114.5108.085.4

B20.0590960.0532580.0577950.05670.105020.101970.100320.102410.9100.358.8

B30.0732270.0742680.070350.07260.125180.121490.115660.120812.1115.162.2

B40.0272110.0282630.0270580.02750.0765890.0761940.0702620.074311.944.639.1

B50.0578820.053680.0601790.05720.107020.11240.11550.111610.5104.566.2

B60.0822020.0835120.0756360.08050.145310.139820.145460.143513.9111.764.7

B70.0565150.0529580.0565730.05530.0951640.0942790.108610.099410.799.458.0

B80.0608880.0617760.0603050.06100.13080.137510.125210.131211.899.669.6

B90.0719760.0717120.0717830.07180.127220.239020.319060.228412.8107.7111.3

B100.0855820.0898920.0934860.08970.0614750.121910.343820.175713.1131.683.9

2015C10.0329660.0273190.0275220.02930.00650210.00550170.0058430.0362420.0353130.0358920.03584.58122.848.8

C20.0274850.0240670.0258070.02580.00818460.00754030.00785250.0350450.0331690.0306620.033013.9135.614.8

C30.0681720.0708630.0597450.06630.0141280.0132980.0132890.0733680.0801380.0755910.076413.5094.435.4

C40.044590.0405990.041570.04230.0118540.0123540.0117980.0651680.0643990.0619610.063814.6655.427.2

C50.0553820.0537130.0483770.05250.0133290.0129090.0123580.0643450.0631610.0682630.065314.1571.328.8

C60.0799040.0785280.0783670.07890.0151490.0148910.0151750.0780470.0720750.0725930.074211.66130.239.8

C70.0274810.0264920.0249990.02630.0080860.00722350.00756050.0519010.0466550.0506540.049710.5248.129.6

C80.0448350.0477960.0421940.04490.00871950.0101210.00881310.0580740.053690.0558360.055911.3975.930.7

C90.0335310.0332870.0356730.03420.01090.0099370.0111520.0447330.0485850.0458320.046415.5742.218.6

C100.0673420.0682070.0611720.06560.0133020.0124790.0139460.0651980.0692190.0688470.067814.1389.330.0

2014B10.116620.110520.114710.11400.0232570.0225550.0225010.0848960.0851330.0919020.0873

B20.0556190.0518790.0520550.05320.0111590.0115970.0106830.0614930.0590020.0653080.0619

B30.0693270.0628960.0637160.06530.0154490.0139920.0140320.0634260.0589170.0648640.0624

B40.0296860.029470.0282210.02910.00979550.0102090.00915430.0433490.0440140.0440090.0438

B50.0634610.0680060.0627820.06470.0128240.0139090.0128980.0576670.0584010.0599740.0587

B60.0858790.0821950.0870330.08500.016030.0163350.0167240.0718630.0753010.0757360.0743

102.779.8

CbE (aNA)CbE (pNPA)Prot

Livermicrosomesl 1/40microsomesl 1/20microsCbE (aNA)CbE(pNPA)microsomes

2013A10.209720.21660.205760.21070.239690.244080.23330.239020.3199.6147.2CbE (aNA)CbE(pNPA)t student0.0000190402

A20.0801840.0941920.0898190.08810.082660.0841290.0853340.084019.487.354.12013153.698.0

A30.122710.119680.112310.11820.0931190.0897580.0876680.090219.8115.057.02014125.372.0

A40.10430.105560.103790.10460.10580.102780.100150.102923.087.556.02015136.257.7

A50.204190.201340.193240.19960.201540.195820.194870.197419.7194.9125.3SEM

A60.116630.118390.109560.11490.146120.137310.150720.144720.9105.586.4201317.551962293412.7565339379

A70.131160.125440.1010.11920.0887550.087630.0813690.085916.7137.564.4201410.29480617515.0902511785

A80.188230.178670.147830.17160.179430.183980.163870.175817.6187.9125.1201518.911761467210.2556833086

A90.233420.234310.228990.23220.223570.225750.24030.229917.7251.8162.0

A100.179560.179060.184170.18090.161360.165720.175750.167620.6169.3101.9

2014B10.126970.135440.12820.13020.116030.11660.118420.117017.4143.984.1

B20.136560.125550.134830.13230.137680.136250.13870.137517.3147.499.6138.475.9

B30.0730110.0724580.0701070.07190.062550.0636170.0626320.062916.285.148.4

B40.0533130.0530340.051840.05270.0650690.0653590.0648350.065115.465.952.8

B50.0737580.0736230.0711120.07280.0755930.0744480.0761540.075415.988.359.4

B60.131230.131280.135170.13260.113610.116180.117920.115918.4138.478.6

B70.132820.135050.130090.13270.0971020.0940810.103830.098317.0149.972.2

B80.118620.109850.115540.11470.0937280.0950180.0931470.094014.5152.681.3

B90.118830.109890.111620.11340.0894490.0961140.0993610.095014.5150.481.8

B100.114910.1040.109530.10950.0762770.0779350.0806230.078316.0131.661.2

2015C10.0733130.0700570.0708080.07140.0434620.0408970.0401880.041510.9125.747.5

C20.0470980.0448850.0422020.04470.0300380.0274350.0242170.027215.356.122.2

C30.237430.243460.254540.24510.19350.195440.193960.194317.5269.9139.0

C40.068080.0656460.0609790.06490.0452050.0424430.0365040.041417.770.529.2

C50.118090.114280.102250.11150.0690610.0746990.0741460.072618.4116.849.5

C60.137080.142140.145320.14150.0773670.0781130.0826260.079416.8162.259.1

C70.103990.104510.101190.10320.0529180.0554720.0669460.058416.7118.743.7

C80.168330.162910.168070.16640.107430.0991480.106320.104318.4174.070.9

C90.137330.139150.141530.13930.0964970.0973470.0963240.096717.6152.468.8

C100.0990040.089230.0913560.09320.0578260.0573280.0601970.058515.5115.347.0

CAT


Activitat CAT

ab

a

b

0.0657179958

0.0494467352

0.0225798613

GPx


Activitat GPx

7.4608596856

5.0106067344

6.304693839

GR


Activitat GR

5.0997577335

3.5662087823

3.9470366008

2.2633155309

3.6447633459

3.606493555

analista:repetim 2013 perq repliques diferents

msole:Dil 1/40. S'ha de repetir

msole:Dil 1/10

CbEs_liver

14.14639306917.098808079410.3484131839

7.74188227676.19982778653.0712135757

17.551962293410.294806175118.9117614672

12.75653393795.090251178510.2556833086

2013

2014

2015


Fetge

a

a

a

ab

b

b

LPO

1g/4+1ml200ul/1000ul

Livercitosolmeanrestatg/mlfactorcoefnmol MDA/g ww

2013A10.11100000140.11680000280.19650000330.1410.09830000250.250.100824.4

A20.08030000330.08229999990.10890000310.0910.04736666880.250.100811.7

A30.1419000030.15019999440.15029999610.1470.10433333120.250.100825.9

A40.08160000290.08259999750.08330000190.0830.03936666750.250.10089.8

A50.0723000020.07249999790.07299999890.0730.02946666630.250.10087.3

A60.09239999950.09380000080.09650000180.0940.05110000070.250.100812.7

A70.08540000020.08680000160.08820000290.0870.04366666820.250.100810.8

A80.09539999810.10090000180.14129999280.1130.06939999760.250.100817.2

A90.06750000270.0696000010.06870000060.0690.02546666810.250.10086.3

A100.25310000780.26739999650.25690001250.2590.21600000560.250.100853.618.0

2014B10.07370000330.07699999960.08070000260.0770.03400000180.250.10088.4

B20.10119999950.10469999910.09700000290.1010.05783333380.250.100814.3

B30.07469999790.07680000360.07850000260.0770.03353333470.250.10088.3

B40.10769999770.11100000140.10970000180.1090.06633333370.250.100816.5

B50.08150000130.08489999920.08380000290.0830.04026666780.250.100810.0

B60.08879999820.09210000190.08969999850.0900.04706666620.250.100811.7

B70.26449999210.2797000110.28249999880.2760.2324333340.250.100857.6

B80.11100000140.11219999940.1124999970.1120.0687666660.250.100817.1

B90.08280000090.08500000090.08669999990.0850.04170000060.250.100810.3

B100.08179999890.08370000120.07580000160.0800.03730000060.250.10089.316.4

MDAmeanrestat

00.04250000040.04380000010.04309999940.04313333330

10.14640000460.14540000260.14450000230.14543333650.1023000032

2.50.28099998830.285499990.2851000130.28386666380.2407333304

50.56319999690.5655999780.57249999050.56709998850.5239666551

101.03110003471.05369997021.04789996151.04423332211.0010999888

12.51.30690002441.30009996891.30369997021.30356665451.2604333212

2015C10.07109999660.08730000260.07739999890.07859999930.03083333250.250.065711.7

C20.10180000220.13120000060.14920000730.12740000340.07963333650.250.065730.3LPOSEM

C30.41549998520.48769998550.47490000720.45936665930.41159999250.250.0657156.6201317.96792352464.4798483346

C40.09160000090.16329999270.16629999880.14039999740.09263333060.250.065735.2201416.35003321264.6975858861

C50.27959999440.28139999510.27349999550.27816666170.23039999480.250.065787.7201576.494164451116.3876901034

C60.10719999670.14630000290.16509999330.1395333310.09176666410.250.065734.9

C70.15209999680.18760000170.19840000570.17936666810.13160000120.250.065750.1

C80.24439999460.27369999890.35580000280.29129999880.24353333190.250.065792.7

C90.14450000230.25249999760.66229999070.35309999680.305333330.250.0657116.2

C100.31430000070.30849999190.69929999110.44069999460.39293332770.250.0657149.576.5

MDAmeanrestat

00.04760000110.04729999970.04839999970.04776666690

10.10559999940.11050000040.10300000010.10636666670.0585999998

2.50.20939999820.20679999890.20790000260.20803333320.1602666664

50.35460001230.35190001130.35310000180.35320000850.3054333416

100.70319998260.70819997790.70359998940.70499998330.6572333165

12.50.8719000220.89270001650.87309998270.87923334040.8314666736

LPO

Hoja3

EROD

LPO

nmol MDA/g pes humit

Activitat LPO

4.4798483346

4.6975858861

16.3876901034

microsomes

EROD 5 min 10 ul ganancia 110 Vol final 250 ul [ER]=2 uM slope 123.9410 ul BFCOD 5 min ganacia 130 vol final 210 [BFC]=200uMslope 59.229ERODBFCOD

LivermicrosomesERODmeanequiv ERBFCODmeanequiv BFCproteinapmol/min/mg protERODBFCOD

2013A150394845.84921.94935.639.822108.62095.52022.42075.579.3820.3049.0582.13201347.078.1

A23032.92818.52751.82867.723.141715.81794.91729.31746.766.8019.4029.8172.30201481.372.5

A35465.35294.65228.15329.343.001527.21510.71498.41512.157.8319.7754.3761.432015152.8177.9

A48182.97725.67875.67928.063.971724.31800.51769.31764.767.4922.9869.5861.6620135.275381421711.6767999771

A55133.15061.55204.15132.941.412317.42364.42322.42334.789.2919.6952.5795.21201412.114086810610.5340544353

A63969.13976.63506.53817.430.801976.51872.51821.61890.272.2920.9436.7672.48201523.10324368617.7699745445

A74082.34118.24091.24097.233.061013.61059.71012.41028.639.3416.6749.5849.56

A85224.14883.44970.45026.040.551782.31800.21805.31795.968.6817.5657.7482.15

A94964.950455545.75185.241.843719.33703.13864.63762.3143.8917.7458.97170.35

A101173.81186.512431201.19.69799.37844.68911.96852.032.5820.5511.7933.30201320142015

2014B111224109591170111294.791.131855.71804.61810.31823.569.7417.40130.9484.17EROD4781.2770037216152.7912332085

B233413335.53361.73346.127.002046.220562037.62046.678.2717.2639.1195.24BFCOD78.056318683872.5484875864177.9405288005

B34106.84145.44038.34096.833.05814.18896.37886.65865.733.1116.2450.8942.81SEM5.275381421712.114086810623.103243686

B47600.97631.88275.97836.263.23616.86741.18775.99711.327.2015.40102.6737.1111.676799977110.534054435317.7699745445

B571957321.77563.67360.159.381374.91460.81462.21432.654.7915.8693.6372.56

B66515.76380.76697.86531.452.701896.619492025.81957.174.8518.4271.5285.33

B73597.23498.63790.83628.929.28717.81719.37761.13732.828.0217.0243.0134.58

B810310113821132611006.088.802486.62582.82621.12563.598.0414.45153.58142.43

B94559.94857.450814832.838.991450.11470.51584.71501.857.4314.5167.1883.12

B104598.148004932.54776.938.54893.95957.851023.7958.536.6616.0060.2448.13

2015C17144.87286.77145.97192.558.03966.21930.07893.02929.835.5610.93132.78136.69

2,5uLmostra+47,5uL bufferC219337205622064120180.0162.8224242468.92489.92460.994.1215.321062.65257.98

C320091177791767318514.3149.381481.91527.11523.21510.757.7817.47213.78138.91

C412829121471198112319.099.391665.21724.117061698.464.9517.69140.44154.18

C5841579207819.18051.464.9621712223.22108.62167.682.9018.3688.46189.65

C616744176571875817719.7142.972301.12308.42326.72312.188.4216.78213.04221.35

C710694113071159011197.090.341805.71755.31780.61780.568.0916.73135.04171.00

C84527.24569.44779.74625.437.32999.51990.49961.83983.937.6318.3950.7385.93

C925181246692549025113.3202.623012.62988.13200.23067.0117.2917.58288.15280.22

C108478.88678.58907.28688.270.101389.31345.21432.81389.153.1215.55112.70143.49

BFCOD

C1863.72759.55815.16966.21930.07893.021508.814681457.7

C21673.61796.11669.224242468.92489.93592.53522.73434

C31090.2985.341025.51481.91527.11523.22218.72124.72074.6

C41311.712551290.71665.21724.117062535.22483.52468.5

C51034.1989.161008.721712223.22108.62836.12836.92852.3

C61456.61474.115392301.12308.42326.73336.13339.23291.2

C7937.52996.51982.381805.71755.31780.623412300.82280.8

C8704.12653.23603.76999.51990.49961.8313911356.41345

C9787.91033.91015.63012.62988.13200.23673.63725.13456.8

C101118.21092.11018.41389.31345.21432.820002007.62110.5

10 ul es curva una mica5 ul millor10 ul i doble de NADPH

LivermicrosomesUDPGT 10uL t=056mean T=010 ul t=5 min56mean t=510 ul t=10 minmean t=10proteinaT=0T=5T=10T=0T=5T=10

2013A1-1958.7-2066.9-1989.5-2005-1276.2-1300.2-1274.2-1284-781.38-687.71-733.76-73420.302.991.911.0920132.31.71.220131.65958490520.0969195875

A2-1918.2-1993.6-2086.7-2000-1279.3-1327.8-1300.5-1303-856.56-819.5-782.42-81919.403.122.031.2820142.51.81.320141.7792587380.0880762605

A3-1093.5-1155.1-1090.3-1113-826.67-847.16-833.2-836-645.44-675.63-658.5-66019.771.701.281.0120152.21.61.220151.57035020240.0573655811

A4-1023.5-1211-1104.3-1113-929.51-938.37-919.43-929-717.27-701.27-704.28-70822.981.461.220.93

A5-1106.6-1141.9-1173.7-1141-892.37-879.65-882.33-885-672.7-643.53-740.33-68619.691.751.361.05

A6-1719.2-1823.7-1665.3-1736-1204.3-1175.4-1124.5-1168-813.3-735.8-774.39-77420.942.511.691.12

A7-1138.3-1139.3-1057.9-1112-869.47-849.39-860.1-860-661.04-633.69-622.35-63916.672.021.561.16694.29

A8-1643.1-1701.7-1635.1-1660-1178.6-1240.6-1241.1-1220-855.19-879.2-863.69-86617.562.862.101.49

A9-1553.6-1201.5-1400.5-1385-1200.4-942.35-957.41-1033-911.17-712.65-707.1-77717.742.361.761.32

A10-1421.2-1849.1-1836.4-1702-1004.9-1217.3-1205.4-1143-740.6-805.43-803.12-78320.552.511.681.15

2014B1-1914.4-1993.4-2087.6-1998-1174.4-1192.8-1248.7-1205-738.93-736.6-751.59-74217.403.472.101.29

B2-1169.9-1186.1-1307.8-1221-882.06-923.78-997.12-934-732.19-748.12-784.64-75517.262.141.641.32

B3-1304.7-1415.1-1321.8-1347-1010.4-1003-958.57-991-751.91-778.21-751.65-76116.242.511.851.42

B4-956.09-953.68-1008-973-719.24-738.57-784.57-747-576.41-591.79-642.99-60415.401.911.471.19

B5-904.18-974.18-997.32-959-759.2-724.06-749.63-744-599.36-635.44-617.26-61715.861.831.421.18

B6-1365.6-1470.4-1549.4-1462-1054.3-1064.2-1057.6-1059-813.17-777.65-761.08-78418.422.401.741.29

B7-878.38-1096.4-1111.2-1029-735.61-879.98-885.94-834-545.05-667.94-662.62-62517.021.831.481.11

B8-1344.2-1367.9-1440.8-1384-933.65-981.14-968.43-961-673.25-645.97-657.68-65914.452.902.011.38

B9-1632.6-1668.7-1712.7-1671-1043.4-1055.5-1097.7-1066-728.74-707.98-710.61-71614.513.482.221.49

B10-1498.6-1532.7-1577.7-1536-972.86-982.29-1017.7-991-661.74-674.53-673.23-67016.002.911.871.27

meanrestat

standard0333635350

0.5383470440431396t=0t=5t=10

18479487818598242013-1496.6-1065.9-744.6

2157615761750163415992014-1358.1-953.2-693.3

4306330672899301029751.1020548321.11825649381.0740766206

857316261616460526017

1610678108051099610826107922015-1172.7-851.3-642.6

Placa transparent s'ha de repetirT=010uL mostraT=5T=10

2015C1-815.31-716.33-798.39-777-654.77-620.9-630.75-635-490.02-448.27-438.93-459

C2-1164.1-1158.4-805.78-1043-867.2-880.47-650.96-800-687.32-688.01-574.54-650

C3-1588.8-1416.4-1409.8-1472-1130.4-895.84-919.45-982-642.11-640.75-657.58-647

C4-944.41-912.53-950.45-936-752.43-755.3-732.49-747-604.38-629.65-630.01-621

C5-1454.2-1468.9-1444.8-1456-913.24-911.57-930.92-919-614.08-601.77-626.54-614

C6-905.1-941.73-1017-955-711.44-690.34-767.93-723-551.51-581.47-574.87-569

C7-1033.2-833.22-933-773.34-713.67-744-631.1-602.45-617

C8-1117.4-308.94-794.84-740-874.24-916.36-552.95-781-714.08-732.91-744.17-730

C9-1313.6-924.06-874.9-1038-1000.1-1054.7-1046.3-1034-746.54-779.65-745.82-757

C10-881.16-899.12-958.78-913-705.19-685.58-692.01-694-579.73-566.34-571-572

Placa negraT=010uL mostraT=5T=10ProteinaT=0T=5T=10

2015C1-896.56-916.41-939.17-917-572.19-653.42-663.81-630-472.98-464.82-513.58-48410.92592596622.541.741.34

C2-1317.9-1212-1340.9-1290-890.02-929-979.19-933-707.06-740.78-751.18-73315.32217122532.551.841.45

C3-1483-1569-1639.6-1564-959.52-996.96-1006.2-988-662.18-675.32-665.66-66817.46913597342.711.711.16

C4-950.29-951.91-1039.7-981-762.61-747.24-768.59-759-592.82-642.34-623.03-61917.69406412531.681.301.06

C5-1510.5-1514.3-1649.6-1558-897.16-936.66-983.83-939-563.57-592.43-585.23-58018.35870077182.571.550.96

C6-1126.6-1044.4-1140.6-1104-932.87-743.79-785-821-596.76-589.02-592.71-59316.77743984641.991.481.07

C7-1030.6-1130.1-821.97-994-753.63-775.17-814.97-781-680.95-607.99-634.15-64116.72501235491.801.411.16

C8-1134.3-1285.7-1283.4-1234-875.96-944.87-979.97-934-715.73-737.08-765.05-73918.39167938772.031.541.22

C9-1390.3-877.04-1040.7-1103-1002.2-1028.9-1016-812.28-730.64-744.85-76317.57990908381.901.751.31

C10-930.95-970.83-1043.2-982-692.62-704.23-744.26-714-604.87-626.72-587.3-60615.54963658151.911.391.18

msole:Doble-check standard preparant-lo de nou.

msole:Sembla que les 3 pendents van be.

msole:Carla ganacia 130 i slope 32.1. Quasi identic pero hem de carregar 210 ul per fer corba per activitat basal.

msole:5 ul perque no es curva

microsomes

standards

Application: Tecan i-controlTecan i-control , 1.6.19.0

Device: infinite 200Serial number: 610000009Serial number of connected stacker:

Firmware: V_2.11_04/08_InfiniTe (Apr 4 2008/14.37.11)MAI, V_2.11_04/08_InfiniTe (Apr 4 2008/14.37.11)

Date:9/3/15

Time:13:19:51

SystemINFINITECAN

UserINFINITECAN\analista

PlateCorning 96 Flat Bottom Black Polystyrol [COS96fb.pdfx]

Plate-ID (Stacker)

Shaking (Linear) Duration:10s

Shaking (Linear) Amplitude:1mm

Label: Label1

ModeFluorescence Top Reading

Excitation Wavelength410nm

Emission Wavelength538nm

Excitation Bandwidth9nm

Emission Bandwidth20nmBFC

Gain130Manual

Number of Flashes10

Integration Time20s

Lag Time0sSystemINFINITECAN

Settle Time0msUserINFINITECAN\analista

Start Time:09/03/2015 13:20:01PlateCorning 96 Flat Bottom Black Polystyrol [COS96fb.pdfx]

MarinaPlate-ID (Stacker)

Temperature: 30.1 Crestatrestat

123456789101112standardmeanmeanrestat vellrestat nou06027699926958141026787.666666666784500Shaking (Linear) Duration:10s

A534143707379424142474752010341143954658109310921044948005101010989216349118351009.6666666667793.33333333332220Shaking (Linear) Amplitude:1mm

B43130731038886541044424953474751085959905612715641983656-61-292101917163917711003138013601775.66666666671247.6666666667988403

C15496708706697086783943464838551020001665190412481285104518561193813245202648205321531443143834652284.66666666672115.333333333314971270

D421335333639128012911255534939464443202545246124261405137614192477140014344524029172891309914321520144529691465.66666666672181621Label: Label1

E11049108351001225262513258752435049504340237823822427124713131294239612851352337806142622461012877298831426155.66666666673002.333333333353682157ModeFluorescence Top Reading

F17941173461746049304854488946404861494780448344534494220123512331447722943433134716011311120171178959556135605711705.66666666676049109185204Excitation Wavelength410nm

G44180451174389297079341938546454246414616088499141933841524536406791094252806633042012 (vell)2015(nou)Emission Wavelength538nm

H5946421524954504842535753vellanovaExcitation Bandwidth9nm

Emission Bandwidth20nm

Gain130Manual

meanrestatNumber of Flashes10

End Time:09/03/2015 13:20:390707379740Integration Time20s

standard 20125388865410554480Lag Time0s

10669708678685611Settle Time0ms

2012801291125512751201Part of PlateA1-G3

4025262513258725422468Start Time:20/03/2015 13:09:27

8049304854488948914817

16097079341938594789404Temperature: 29.9 C

123meanrestaconcentracioresta

meanrestatA195187165182.3333333333000.0

Carla03173484683780B256248236246.666666666764.3333333333564.3

5636592623617239C378342378366183.666666666710183.7

10689799682723346MontsenouvellrestatrestatD583629647619.6666666667437.333333333320437.3

201173993102310636850817868919310075.666666666794.666666666700E1193114412131183.33333333331001401001.0

40161515931590159912225441411441421450416431429355.3333333333334.3333333333F2183242221972267.33333333332085802085.0

8028932861288328792501slope 32.1410589530540817766827553803.3333333333477.3333333333708.6666666667G4300438545104398.333333333342161604216.0

16055435675546855625184207467387361483147614137401457.3333333333664.33333333331362.6666666667Placa nova

401349138613832874286327731372.66666666672836.666666666712972742

802734263826605609556556102677.33333333335594.66666666672601.66666666675500

160521953775283111081084810877529310944.33333333335217.333333333310849.6666666667End Time:20/03/2015 13:09:38

Label: Label1nou4646vell5851

ModeFluorescence Top ReadingER





Gain110Manual

Number of Flashes10

Integration Time20s

Lag Time0s

Settle Time0ms

Part of PlateA1-H3

Start Time:10/03/2015 9:49:14

Temperature: 20.3 C

123std ERODRestat

A1691811720174-691

B8198288285825-40

C140314601456101439.6666666667574.6666666667

D2625255426062025951730

E526152404914405138.33333333334273.3333333333

F9885100971011480100329167

G2031520142196481602003519170

H

End Time:10/03/2015 9:49:27

Wait (Plate)OnTarget Temperature: 30 C

Label: Label1

ModeFluorescence Top Reading





Gain70Manual

Number of Flashes10

Integration Time20sMU

Lag Time0s

Settle Time0ms

Part of PlateA4-G6

Start Time:11/03/2015 12:52:00

mean

033363535

0.5383470440431

1847948781859

21576157617501634

43063306728993010

85731626161646052

1610678108051099610826

2013

2014

2015

pmol/min/mg protena

Activitats CYP

a

a

a

a

b

b

5.2753814217

12.1140868106

23.103243686

11.6767999771

10.5340544353

17.7699745445

UDPGT


Activitat UDPGT

0.0969195875

0.0880762605

0.0573655811

Tecan.At.Common, 1.6.19.0Tecan.At.Common.Controls, 1.6.19.0Tecan.At.Common.Dialogs, 1.6.19.0Tecan.At.Common.MCS, 1.6.19.0Tecan.At.Common.Results, 1.6.19.0Tecan.At.Communication.Common, 1.6.19.0Tecan.At.Communication.Port.RS232, 1.6.19.0Tecan.At.Communication.Port.SIM.Common, 1.6.19.0Tecan.At.Communication.Port.USB, 1.6.19.0Tecan.At.Communication.Server, 1.6.19.0Tecan.At.Communication.SIM.AMR, 1.6.19.0Tecan.At.Communication.SIM.Connect, 1.6.19.0Tecan.At.Communication.SIM.GeniosUltra, 1.6.19.0Tecan.At.Communication.SIM.Safire3, 1.6.19.0Tecan.At.DocumentManagement, 1.6.19.0Tecan.At.DocumentManagement.Reader, 1.6.19.0Tecan.At.FileManagement, 1.6.19.0Tecan.At.Instrument.Common, 1.6.19.0Tecan.At.Instrument.Common.Reader, 1.6.19.0Tecan.At.Instrument.Common.Stacker, 1.6.19.0Tecan.At.Instrument.Reader.AMR, 1.6.19.0Tecan.At.Instrument.Reader.GeniosUltra, 1.6.19.0Tecan.At.Instrument.Reader.Safire3, 1.6.19.0Tecan.At.Instrument.Server, 1.6.19.0Tecan.At.Instrument.Stacker.Connect, 1.6.19.0Tecan.At.Measurement.BuiltInTest.Common, 1.6.19.0Tecan.At.Measurement.Common, 1.6.19.0Tecan.At.Measurement.Server, 1.6.19.0Tecan.At.Stacker.Server, 1.6.19.0Tecan.At.Win32, 1.6.19.0Tecan.At.XFluor, 1.6.19.0Tecan.At.XFluor.Connect.Reader, 1.6.19.0Tecan.At.XFluor.Core, 1.6.19.0Tecan.At.XFluor.Device, 1.6.19.0Tecan.At.XFluor.Device.AMR, 1.6.19.0Tecan.At.XFluor.Device.GeniosUltra, 1.6.19.0Tecan.At.XFluor.Device.Reader, 1.6.19.0Tecan.At.XFluor.Device.Safire3, 1.6.19.0Tecan.At.XFluor.Editor, 1.6.19.0Tecan.At.XFluor.ExcelOutput, 1.6.19.0Tecan.At.XFluor.ReaderEditor, 1.6.19.0Tecan.At.XmlSettings, 1.6.19.0

EHC, V_2.11_04/08_InfiniTe (Apr 4 2008/14.37.11)MTP, V_2.11_04/08_InfiniTe (Apr 4 2008/14.37.11)CUV, V_2.11_04/08_InfiniTe (Apr 4 2008/14.37.11)HCP, V_2.02_05/06_HCP (May 23 2006/14.05.27)MEM, V_2.12_03/08_MCR (Apr 3 2008/16.03.31)MEX, V_2.12_03/08_MCR (Apr 4 2008/14.29.44)

standards

0

480.3333333333

611

1201.3333333333

2468

4817

9403.6666666667

farmacs

basal sense incubar 30 min50394845.84921.95133.15061.55204.13969.13976.63506.54964.950455545.7mean%%ERODBFCODCbE (pNPA)SEM

5 minEROD (10 ul)A1A5A6A9T studentmeanActivInhibicioEROD 10 ulA3A7A8A10T studentA1A5A6A9A3A7A8A10A1A5A6A9A3A7A8A10B3B4B6B7B3B4B6B7B3B4B6B7A1A5A6A9A3A7A8A10meansemAcetaminophen92.409856449480.110414006196.54606488091.5226027752.61573941591.4148313859

Control5703.15393.65490.579728282.47860.657406341.55966.17745.98754.48953.57017.01000buffer5213.85126.35026.44381.94677.3513567866807.77057.4767.93793.82860.725529803860168485512247316884807100100100100100100100100buffer5690.85525.25592.45801.358906276.17002.17301.473571809.51844.62043.356035989722018991001001001001001001001001001001001001000ControlIbuprofen83.575414041461.932095703597.65655257090.98855369664.37426709862.2903345634

NF1376.92519.41565.33032.12432.82885.71173.31032.1911.772423.81993.61455.80.00000000011900.22773aNF571.45538.43399.17300.21290.1296.12363.04345.69311.5377.45779.39580.610.000004178118212784103919585032953407932.934.617.323.19.86.24.99.8aNF1851.411921444.91667.41585.61221.92178.92047.31707.3236.94233.64248.1614961492197824026.724.927.412.632.934.617.323.19.86.24.99.819.23.0NFCarbamazepine87.68358720468.12873942490.40107710021.67588459244.06058935672.0770491536

aceto4565.24274.950047470.468007294.550155596.35578.97556.17642.68111.90.17903164726242.58911aceto45654374.64370.94512.94490.64644.96510.56777.76596.8775.85779.25851.80.7627156936461571885397777044374549662880283.589.489.791.686.696.296.399.4acetomino5019.34938.84871.15941.75765.35706.46570.76529.86633.91841.518352024.9494358046578190088.296.991.1100.183.589.489.791.686.696.296.399.492.41.5acetoPropanolol84.400042576361.01893444292.85428828881.95189497574.66494622682.951084999

IBU4368.54370.44598.66903.5694074005123.148045216.56759.67026.67191.60.04524507565891.98416ibu4429.64314.44291.84332.14068.23953.25754.66035.46344.8630.1639.54665.080.5113603933444670815048699343454118604564580.488.183.982.484.887.087.879.9ibu4804.44594.94624.14951.94986.94981.861656124.66213.31424.91407.81533.7467449746168145583.483.085.476.680.488.183.982.484.887.087.879.983.61.0IBUSimvastatine81.83666315562.678863765163.29299698181.96158263974.14494496293.442536586

CBZ5066.446284788.37429.16957.27225.65319.75528.25455.776167629.88021.10.20012561586305.49010cbz4454.34149.44215.34350.74390.14265.75967.56087.96422.9772.54806.41813.460.5842948448482872045435775642734336615979787.389.690.391.483.491.689.598.8CBZ4889.44798.74715.45121.64923.85142.46047.46189.16223.613651405.31490.6480150636153142085.784.585.274.887.389.690.391.483.491.689.598.887.71.7CBZFenofibrate98.789257192681.154904635153.32854148934.46020064042.98483066482.9445490782

propan4935.54791.15133.76864.87221.771725139.64865.65058.67110.87279.57188.40.07561879536063.48614propan4045.33913.83935.84131.94117.44039.65787.86100.75967.6795.71818.1824.190.4441699157495370865021719339654096595281389.688.283.584.877.486.686.5100.6propa41804314.54294.94856.84721.64812.25873.75972.15884.91445.71420.91559.3426347975910147576.180.181.977.789.688.283.584.877.486.686.5100.684.42.0propanLoperamide87.919559130877.422512529392.8593653791.53015716255.04799118552.6856288437

SIMV5125.34793.84922.27158.87172.76913.75013.95227.25126.27299.26922.37435.90.08291076196092.68713simv3897.23765.13827.84225.73988.64086.76152.36101.45715637.14602.42676.670.409278364494770825122721938304100599063989.588.185.185.174.886.787.079.1simvas4531.94358.64411.54748.34739.34811.85872.66000.75999.11243.51267.71217.6443447665957124379.179.682.565.489.588.185.185.174.886.787.079.181.82.0SIMVMethylparaben80.669682791779.339293236991.96191939970.9437861881.93041110232.9359586396

Feno5232.65297.25612.88205.98759.38310.95096.55354.45188.57828.77657.98665.70.68152052086767.5964feno6762.26691.36760.14547.34587.74190.97037.97497.57666.4935.3948.991009.50.6268404295538184255213805167384442740196597.3104.886.794.9131.593.9107.5119.5fenof5011.54917.94791.25485.65597.95870.46719.66859.86861.11323.51319.51521.6490756516814138887.694.494.473.197.3104.886.794.9131.593.9107.5119.598.84.5FenoBenzotriazol82.240116686383.679089849799.75454364662.16595674020.65979187291.4801236611

meanNaproxene83.578826741695.068975585498.12169011272.07072190571.84318918851.7280918776

A2B2B3B4T studentmeanActivInhibicioB5B8B9B10T studentA2B2B3B4B5B8B9B10A2B2B3B4B5B8B9B10meanSEMNF & Keto19.193822073652.03.00500082494.8656502649

control37593680.53671.63758392240725111.85091.3496649025153.955574470.41000control7634.872047396.31046011493116985022.64916.64991.84210.64365.44655.7370439175056520474121121749774411100100100100100100100100control1000Diclorvos10.07157953481.7624589837

alfa456.35490.07329.12443.15438.73332.1555.59478.47362.46570.23527.51528.390459.31090alfa753.94514.04687.371891.311471660.4609.41664.41804.35183.27302.45370.880.0000001694425405466542652156669328611.510.39.210.48.814.013.96.5alfa 10 uM10.60.9

loperamide3617.23436.83451.13513.43619.93632.54168.14542.147044378.94268.14787.50.08402115714010.09010loperamide6524.66044.35929.18899.89478.19241.241784264.94438.939403936.94187.80.30327446393502358944714478616692064294402294.591.688.486.083.282.186.391.2loperamide 100 uM87.91.5

metilparaben3196.33038.63030.33397.332773362.443454153.4382541904073.743950.00587101223690.38317metilparaben5928.55597.25959.78507.58848.88722.53899.13891.239673398.93457.33645.40.15148859143088334641084220582886933919350183.485.481.281.178.677.578.779.4metilparaben80.70.9

benzotriazol3308.43108.83284.435323447.43441.84309.43671.44136.93670.33603.23625.30.00090930273594.98020benzotriazol58455946.157279636.39316.89581.34247.64288.44357.73290.33651.73948.20.2783272483234347440393633583995114298363087.388.779.969.878.884.886.482.3benzotriazol82.22.2

naproxen3206.63228.73195.53564.33701.93572.94303.84342.94561.54126.63934.24686.80.02900656163868.88713naproxen6285.85928.65749.665228296.69462.14247.54074.44330.73434.637383851.30.13900241513210361344034249598880944218367586.792.287.181.680.872.284.783.3naproxen83.62.1

basal sense incubar 30 min1782.31800.21805.32317.42364.42322.41976.51872.51821.63719.33703.13864.6mean%%

5 minBFCOD (10 ul)A8A5A6A9T studentActivInhibicioBFCOD 10 ulA3A8A5A10T studentA8A5A6A9A3A8A5A10A8A5A6A9A3A8A5A10B3B4B6B7B3B4B6B7B3B4B6B7A8A5A6A9A3A8A5A10meansem

Control1872.21734.91714.220982055.621101829.11441.515433904.74005.42917.62268.91000buffer2056.91808.91923.93429.82425.92956.42224.62107.42078.2802.34718.12637.211774208816053609193029372137719100100100100100100100100buffer477.35452.52418.47358337.07346.49849.22783.58718.63264.94302.24294.624493477842871001001001001001001001001001001001001000Control

keto860.04752.26826.541085.81020.91020.6784.22739.81760.331864.618891853.70.00057308761121.54951keto11921204.91274.41518.217381710.21113.11092.71091.2363.34366.23348.610.00720112468131042761186912241655109935945.849.947.551.863.456.451.450.0keto357.19343.14359.25304.59278.03291.61538.96573.01539.57218.38220.35237.0235329155122578.683.970.278.445.849.947.551.863.456.451.450.052.04.8656502649keto

aceto1363.51383.91318.81748.41706.91868.41203.71277.11323.52667.42569.92714.50.10699243051762.27822aceto1640.716921669.22445.32132.82441.91664.61735.81791.3588.8559.87576.520.2744009231135517751268265116672340173157576.485.079.073.486.479.781.080.0aceto442.26429.2405.56320.66319.78333.19730.61713.64722.32278.14256.84270.8742632572226994.793.592.193.576.485.079.073.486.479.781.080.080.12.6157394159aceto

IBU10861030.11038.51530.41583.91210.5723.47821.25754.411398.41875.21865.60.0012681761243.15545ibu1533.51429.81503.21544.71825.22309.31553.31516.11496.5395.19413466.540.060057174210521442766171314891893152242559.369.047.847.577.164.471.259.1ibu326.15347.3357.06317.48292.01302.4565.09592.71591.61226.7207.4236.6134430458322476.487.674.477.859.369.047.847.577.164.471.259.161.94.3742670986IBU

CBZ986.61941.741115.7986.361152.21511963.751131.412061956.92008.122040.00373341961347.05941cbz1579.61497.81644.92159.71755.52186.61588.31461.71582587.91602.55555.910.1079266821101512171100205615742034154458257.258.368.657.081.669.272.380.9cbz379.82387.43397.23294.68299.9270.13620.19656.67652.81239.54254.39257.2938828864325086.483.082.187.257.258.368.657.081.669.272.380.968.14.0605893567CBZ

propan782.331069.31152.1765.43922.03970.24825.911161.31117.11674.11693.322040.00096321431194.85347propan1443.61425.91577.51109.71532.21509.71417.51531.41522.2561.86573.68583.190.018901041210018861035185714821384149057356.442.464.551.576.847.169.779.7propan356.18287.89329.3232.39285.27261.35656.69519.2223.14221.15241.3732426058822972.274.875.079.656.442.464.551.576.847.169.779.761.04.6649462268propan

SIMV1190.11078.211431427.31505.61465.1943.521016.3922.982162.423842328.90.01245247531464.06535simv1330.81283.81217.92035.91829.51842.31330.21186.9393.43406.29362.320.029015760611371466961229212781903125938764.170.259.963.566.264.858.953.9simv423.31337.55391.54335.78308.98315.315.9331-3.5359-1.4545219.88217.75229.5738432022285.592.277.464.170.259.963.566.264.858.953.962.74.1449449629SIMV

Feno1454.61339.31515.51549.51594.81576.8944.8414091258.62995.82901.92921.70.15458878291788.57921feno1699.91896.31620.22231.81927.5219

The use of Solea spp. as sentinel species in Europe · (2016). The use of juvenile Solea solea as...

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