The PinAAcle Family of AAS Features and Benefitsper-form.hu/wp-content/uploads/2016/10/ric_04... ·...
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11 © 2009 Perkin Elmer
The PinAAcle Family of AASFeatures and Benefits
Riccardo MagariniBudapest 2016, October 17th
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IonizationExcitationAtomizationVaporization
M* M+ + e_
Mo M*
MA Mo + Ao
Solution Solid
Flame Atomic Absorption
• Fast◦ 3 – 5” per determination
• Low analytical costs• Easy to use• Single element technique
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PerkinElmer PinAAcle platform
• Real Time Double Beam• State of the art optics with fiber optics• Customized solid state detector
• Automatic gas control◦ Oxidant and Fuel Only Mixed in Spray Chamber
• Optimized sample introduction system◦ Inert to acid, alkalis and organics◦ Solid titanium burner head◦ Adjustable nebulizers
• EDL lamps
• HGA with D2 background correction• THGA with Zeeman background correction• FIAS and FI-FU (Fias-Furnace)
• Syngistix Software Platform
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PinAAcle 900 platform (models F; H; T)
Intuitive Syngistix +Windows 7
Removable Burner Assembly
TubeView color furnace camera
Graphite Furnace
AS 900 furnace auto-sampler
Innovative new mixing chamber
Small flame/furnace AA
footprint
HCL and EDL PerkinElmer lamps
Eight-lamps mount
Cutting-edge fiber optics
Stacked flame/furnace design
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The PinAAcle™ 500 Flame AA Spectrometer
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Optical System with Fiber Optics
Unique !
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PinAAcle: Unique innovAAtive fiber optics design!
• 1) High Light transmission due to fiber optics and unique possibility of fiber beam shaping
• 2) Does not get “dirty” over time like mirrors which leads to less cleaning and maintenance of mirrors.
• 3) Fiber optics makes it possible to design a small footprint instrument, with high performance true double beam optics.
Best light transmission with minimum maintenance and
compAAct footprint
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PinAAcle: Unique innovAAtive fiber optics design!
Light from lamp
Light from lamp captured by fiber optics
Conventional “slit” shapes the beam.
Lost light intensity
Fiber optics reshaped from circular to “slit” shape, transmitting ALL light.
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Customized Solid-State Detector
• Integrated low noise CMOS charge amplifier array◦ Exceptional signal-to-noise and Quantum Efficiency◦ Improves signal throughput, lower background and
therefore better DLs• Performances
◦ Traditional PMT’s performance decrease as the detector ages. This does not happen with solid state
Photomultiplier Solid state detector
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Electrodeless Discharge Lamps
Lower cost of ownership; Better DLs.
• Longer lifetime than HCL• Lower cost of ownership• Better S/N ratio for better DL• One universal starter (lamp
driver) for all EDL elements• Intelligent pre-warm up for
best time efficiency
Cd 0.1 µg/LCd 0.1 µg/L
EDL HCL
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Flame Gas Flow Optimization
Unique !
User Benefits: Element specific Optimized results Ease of use Reproducible
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Burner Head Optimization – Burner Alignment (Pin900)
User Benefits: Easy to use – can
be recalled when stored
Optimized results Convenience
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PinAAcle 900, Stacked Atomizers
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Graphite Furnace - Atomic Absorption (GF-AAS)
• Low DLs (g/L) for many elements• Slower than flame (45 - 90 seconds)• Little sample consumption (L) • Single element technique• Some experience is needed
.
Chromium, 10 µL of 1 µg/L
0.00
0.02
0.04
0.06
0 1 2 3 4 5 6
Time / s
Abs
orba
nce AA-BG
BG
Tem
p
Time
Dry 1
Pyrolysis
AtomizationClean
Dry2
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Patented Transversely Heated Graphite Atomizer
.
Atomization Temperatures HGA /THGA furnaces
Temperature profiles for HGA and THGA tubes
HGA °C THGA °C
As 2300 2000Al 2500 2300B 2650 2500
Ba 2550 2300Cu 2300 2000Cr 2500 2300Fe 2400 2100Ni 2500 2300Pb 1800 1600Si 2650 2350Sn 2300 2200Ti 2650 2500V 2650 2400
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GF technology improvements: temperature distribution
HGAMassman-style atomizer
THGATransverse-heated atomizer
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Zeeman effect properly corrects also non uniform bkgd. (some molecular bands create structured bkgd.)
Bkgd. Correction: Continuum vs Zeeman(Se in fish tissue)
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Longitudinal Zeeman Background Correction
• With Polarizer◦ “Old” Zeeman
configuration◦ Needs a polarizer for
background discrimination
- Energy loss >50%◦ PMT detector
• Without Polarizer◦ Better Zeeman
configuration◦ No polarizer for
background discrimination
- No energy loss ◦ SSD detector
0.012
Light SourceAtomization
Modulation
Monochromator
DetectorPMT
DisplayPolarizer
Magnet
Light Source Atomization
Modulation
Monochromator
DetectorSSD
Display
Magnet
AC Zeeman Magnet Field orthogonal to optical beam
Longitudinal AC Zeeman Magnet Field parallel to optical beamLongitudinal Magnetic Field
Transversal Magnetic Field
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Zeeman bkgd. correction
As std. 30 ug/L As 30 ug/L + Al 50 mg/L
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Longitudinal Zeeman bkgd. correction
As 1 ug/L As 1 ug/L + Al 200 mg/L
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Stabilized Temperature Platform Furnace
The STPF protocol.
Parameter /Feature Benefit Proof Statements
Platform atomization Integrated platforms
THGA (with & without endcaps) Integrated platform HGA tubes Uniform pyrolytic graphite coatings
Isothermal atomization Ease of use (no changing platforms) Reproducible & reliable data All elements from platform Longer tube lifetimes (corrosive samples)
Gas-phase temperature measurements (CARS) Reduced or eliminated chemical interferences Characteristic mass (m0) stability Lifetime studies Scientific publications
Rapid, controlled furnace heating Independent of line voltage, tube parameters (i.e., resistance) True Temperature Control (TTC)
Reliable day-to-day performance Temperature measurements Refractory element performance with different voltages
Integrated Absorbance (peak area) measurements
More robust measurements Reliable day-to-day performance Independent of matrix changes
Peak area vs peak height studies
Zeeman-effect Background Correction
No user selection required Wavelength independent method Better detection limits
Interference studies Scientific publications
‘Universal’ Chemical Modifier Palladium/Magnesium nitrate
Faster, simpler method development Reproducible & reliable data
Interference studies Recovery measurements Scientific publications
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0.610
0 5.00Time (sec)
1400 oC
1600 oC
1800 oC2200 oC
Atomization Temperature and Signal (Pb-HGA)
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TubeView Furnace Camera
• Color TubeViewFurnace Camera
• Probe Depth Alignment
• Drying and Pyrolysis monitoring
User Benefits: Precision Method development Reproducible results
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Accessories
• S10 Auto-sampler• Flow Injection-MHS AA• Flow Injection Flame AA• FIAS-Furnace Coupling• AutoPrep 50• FAST Flame Sample Automation
Platform
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CV /Hydride generation
.
• Very good DLs (ug/L)• Useful for
◦ Hg; As; Sb; Se; Te; Bi; Sn◦ Typical hydrides:
- MeH2, MeH3, MeH4
- i.e. AsH3, SbH3, SeH2, TeH2, BiH3, SnH4, GeH4
• Produced by hydrogen generated from ◦ NaBH4 reactions with HCl
• The only AAS technique that is chemistry dependent
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Flow Injection Mercury Systems - FIMS
.
• Dedicated Hg analyzer• Determines Hg via CV-AA• Stand alone system• Fully automated• Easy to operate
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Hg determination with pre-concentration by Amalgamation
FIMS-100.
Sample Concentration ng/L Recovery (%)*Tap Water 1 0.55 (0.10) 105Tap Water 2 0.72 (0.14) 103Harbour Water 1 2.93 (0.07) 89Harbour Water 2 2.89 (0.06) 92Waste WaterTreatment Plant
0.69 (0.02) 111
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FIAS-Furnace Coupling
.
• Features◦ Separation of matrix
and analytes◦ Controlled atomization◦ Fully automated◦ Easy to use◦ Integrated software
Benefits Reliable analysis of
difficult elements in very complex and difficult samples
Improved detection limits
More reliable analysis
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SMS 100, dedicated Hg analyzer in solids and liquids
Direct Hg determination in solids /liquids samples without any sample prep
• Soils• Sediments• Geologicals• Coal• Foundry scrap• Plastics• Sewage/ Sludge
• Blood• Paints• Animal Tissues• Marine Tissues• Tobacco• Vegetation• Cement
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Matrix Independent Analysis
Calibration using a variety of Certified reference Materials (CRM)
• Calibrate once for many sample types.
• No matrix effects are found when the calibration curve is created using certified reference materials as standards.
• see EPA GRAPHS…
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FAST Flame 2 with SC2 Autosampler (2-trays)
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FAST Flame 2 – Schematic Diagram
• Pump 1 carries sample• Pump 2 delivers diluent
◦ Pump 1 + Pump 2 = Constant◦ DF = (Pump 1 + Pump 2) /Pump 1
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FAST Flame Sample Automation Platform
AAPrep2
FAST Flame 1
FAST Flame 2
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Titan MPS™ Sample Preparation System
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The right vessel for the right application
• Standard 75mL vessel◦ 16-vessels configuration◦ For a majority of applications
• High Pressure 100 mL vessel◦ 8-vessels configuration◦ For high pressure applications
260°C Temperature 260°C40 Bar Pressure 100 Bar75 mL Volume 100 mL
PTFE-TFM Material PTFE-TFM
All vessels come with a 1 year warranty
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Sophisticated sensing technologyEffortless contact-free and connection-free optical sensing
• Direct Temperature Control (DTC)™◦ Reference quality sample temp.
monitoring for every vessel◦ Unsurpassed temperature control
• Direct Pressure Control (DPC)™◦ Reference vessel pressure sensing◦ Simple, inexpensive and robust
Intelligently filter out the IR signal of the vessel, allowing direct measurement of the sample temperature without
interference.
A reference beam of light is sent through a prism and glass ring. Pressure on the glass ring is measured via
the shift in reference beam wavelength.
Theory of OperationDTC™ DPC™
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Digestion data exporting for analysis, reporting and archiving
• Export data to an easily readable, common numeric format• Import into Excel or use the included Windows based DataViewer
Placeholder,
Need Excel example as well
Excel
DataViewer
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Titan and Safety
• Hardware interlocks◦ Lid, power supply, inverters, magnetrons, oven body
• Software interlocks◦ Lid, DTC, DPC, vessel venting
• Vessel burst resistance◦ Elastic, gas containment manifold
• CE/NRTL/FCC certification
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Hot Plates (Open Digestion)
The Good:• Cheap• Easy methods• Large sample volumes
The Bad:• Not suitable for Mercury• Corrosion, short lifetime• Poor Temperature Control• Contamination of Sample• Safety
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Sample Preparation Block
• An acid resistant graphite heating block◦ Reduced sample contamination
• Corrosion - Teflon coated, Kydex, Corian• Temperature Uniformity - Heating Material• Sample temperature control – SPB probe• Number of samples - 24; 48 or 72
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DigiTUBE
.
Watch Glass
Labeling
Rack LockScrew Cap
Graduated cylinder
Class ARefluxing
Level of conc acid
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Typical Application For Touch Screen Controller
.
• Touch Screen Controller◦ Ideal for complex digestions at multiple temperatures◦ Ideal for method development ◦ Typical Samples
- Animal feeds, pet foods, human foods- Biological samples- Plastics
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Thank you for your attention.
? Any questions ?
Riccardo MagariniSr. Specialist Atomic Spectroscopy [email protected]