The New age Shower James Diewald Michelle Cisar Chris Jones Paul Georgadis.

The New age The New age ShowerShowerJames DiewaldJames Diewald

Michelle CisarMichelle Cisar

Chris JonesChris Jones

Paul GeorgadisPaul Georgadis

The ExperimentThe Experiment

Bacteria are everywhere.Bacteria are everywhere. After collecting and identifying After collecting and identifying

bacteria collected from the entire bacteria collected from the entire western campus we decided to western campus we decided to narrow our search to one bathroom.narrow our search to one bathroom.

That bathroom is 3 South Peabody That bathroom is 3 South Peabody Hall.Hall.

Samples will be taken from Samples will be taken from designated sights in the bathroom.designated sights in the bathroom.

The Essential The Essential BackgroundBackground

Most domestic bacteria ar harmless but some Most domestic bacteria ar harmless but some rare strains can cause death.rare strains can cause death.

Bacteria are the most common organisms on Bacteria are the most common organisms on earth. earth.

Bacteria are 80% water.Bacteria are 80% water. Every one of you has bacteria inside of youEvery one of you has bacteria inside of you Bacteria prosper in warm moist places, Bacteria prosper in warm moist places,

preferably between 25 and 35 degrees C (your preferably between 25 and 35 degrees C (your body temperature).body temperature).

Your bacteria are good for you.Your bacteria are good for you. High volume bathrooms are bacteria breeding High volume bathrooms are bacteria breeding

grounds.grounds.

What you Need to KnowWhat you Need to Know The decided sampling The decided sampling

locations are as locations are as follows.follows.

1. The toilet seat, flush 1. The toilet seat, flush handle and handle and

outgoing outgoing door latch.door latch.

2. The outgoing door 2. The outgoing door handle.handle.

3. The sink bowl and 3. The sink bowl and knobs.knobs.

4. The shower floor 4. The shower floor and and knobs.knobs.

We expect a We expect a number of bacteria number of bacteria to be thriving at to be thriving at these prescribed these prescribed locations.locations.

All of which are All of which are thought to be thought to be infectious to infectious to humans and some humans and some may be deadly.may be deadly.

The SuspectsThe Suspects

The cell wall is The cell wall is composed of composed of peptidoglycan, a peptidoglycan, a protein-sugar protein-sugar molecule. molecule.

No conventional No conventional nucleus.nucleus.

Uses flagellum for Uses flagellum for movement.movement.

The Suspects (1)The Suspects (1)

StaphylococciStaphylococci-yellow, spherical -yellow, spherical

cells in irregular cells in irregular clustersclusters

-carried by 15-20% of -carried by 15-20% of populationpopulation

-common infections:-common infections:

hair follicle, bone, hair follicle, bone, intestine, urinary intestine, urinary tracttract


StreptococciStreptococci-small white, semi--small white, semi-

transparent, transparent, chains of spherical chains of spherical cellscells

-very common-very common


throat, skin, joints, throat, skin, joints, heartheart


E-coliE-coli-smooth, orange, -smooth, orange,

spherical cellsspherical cells-part of normal flora -part of normal flora

of intestinal tractof intestinal tract-common infections:-common infections:

intestines, intestines, traveler’s diarrhea, traveler’s diarrhea, fatal cholera-like fatal cholera-like diseasedisease


SalmonellaSalmonella-larger, tan, -larger, tan,

smooth cellssmooth cells-transmitted -transmitted

through through contaminated contaminated food and waterfood and water

-common -common infections:infections:gastrointestinal gastrointestinal tract, abdomentract, abdomen


CampylobactorCampylobactor-white, curved rods-white, curved rods

-ranks with E-coli as -ranks with E-coli as major cause of major cause of diarrhea in worlddiarrhea in world


intestinal tract, intestinal tract, diarrheadiarrhea

Further ExpectationsFurther Expectations

We expect the We expect the shower and the door shower and the door handle to have the handle to have the highest levels of highest levels of bacteria.bacteria.

The shower bacteria The shower bacteria levels will be high levels will be high due to traffic and due to traffic and moisture levels that moisture levels that advocate bacteria advocate bacteria growth.growth.

The door handle is The door handle is the single most the single most touched object in touched object in the bathroom and the bathroom and may therefore have may therefore have high levels of high levels of bacteria.bacteria.

We also expect a We also expect a significant significant difference before difference before and after cleaning.and after cleaning.

MaterialsMaterials

16 Agar Plates Cotton Swabs Permanent Marker Tape (to seal agar after sampling)

Sampling ProceduresSampling Procedures We took before and We took before and

after cleaning after cleaning samples on each of samples on each of two days from the two days from the named locations.named locations.

A specific “Circle A specific “Circle X” plating method X” plating method was created in was created in order to insure a order to insure a uniform bacteria uniform bacteria distribution on the distribution on the agar plates.agar plates.

To calculate the To calculate the percent coverage percent coverage of each species, we of each species, we utilized Photoshop utilized Photoshop to lay a grid over a to lay a grid over a series of digital series of digital photos.photos.

The photo was The photo was zoomed in on and zoomed in on and the percentage the percentage was established.was established.

Mission ResultsMission Results

As before, bacteria are everywhere.As before, bacteria are everywhere. The following graphs will provide The following graphs will provide

detailed data on our results.detailed data on our results.

Debrief (1)Debrief (1)

Basic Data OverviewBefore 11/13 Before 11/14 After 11/13 After 11/14

Location→ T SH SI D T SH SI D T SH SI D T SH SI DTotal %→ 2 79 78 97 1 94 43 2 1 80 2 4 4 99 1 0Species↓YellowColonies

.7 52 4 .5 2 24 1 41 2 1.5 16 16

WhiteColonies

1 12 6 .5 3 16 .5 1 11 2 1 2 19 .5 19

TanColonies

.3 9 68 89 3 .5 23 1 .5

SmallWhiteColonies

97 60 60

OrangeColonies

6 5 .5 4 4


0

10

20

30

40

50

60

70

80

90

100

Percent Coverage

Toilet Shower Sink Door

Location

Total Coverage Percentages by Location

Before (11/13)

Before (11/14)

After (11/13)

After (11/14)


Total Percent Coverage by Cleaning

0

20

40

60

80

100

120

Before (11/13) Before (11/14) After (11/13) After (11/14)

Cleaning Dates

Percent Coverage

Toilet

Shower

Sink

Door


0

10

20

30

40

50

60

70

80

90

100

Percent Coverage


Location

Before Cleaning 11/13

Yellow Colonies

White Colonies

Tan Colonies

Small White Colonies

Orange Colonies


0

10

20

30

40

50

60

70

80

90

Percent Coverage


Location

Before Cleaning 11/14

Yellow Colonies

White Colonies

Tan Colonies


Orange Colonies


0

5

10

15

20

25

30

35

40

45

Percent Coverage


Location

After Cleaning 11/13

Yellow Colonies

White Colonies

Tan Colonies


Orange Colonies


0

10

20

30

40

50

60

Percent Coverage


Location

After Cleaning 11/14

Yellow Colonies

White Colonies

Tan Colonies


Orange Colonies

Final ConclusionsFinal Conclusions

Statistical analysis via Statview Statistical analysis via Statview showed no significant difference showed no significant difference before and after cleaning.before and after cleaning.

Our graphs did show otherwise.Our graphs did show otherwise. We assume that a larger data set We assume that a larger data set

would yeild our predicted results.would yeild our predicted results.

The New age Shower James Diewald Michelle Cisar Chris Jones Paul Georgadis.

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Transcript of The New age Shower James Diewald Michelle Cisar Chris Jones Paul Georgadis.