The innate immune system as a window into sponge-microbe interactions
description
Transcript of The innate immune system as a window into sponge-microbe interactions
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The innate immune system as a window into sponge-microbe
interactions
Jo BayesSandie Degnan
School of Biological SciencesUQ
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Questions
1. How does Amphimedon acquire its symbionts?
2. How does the bacterial community of Amphimedon change throughout its lifecycle?
3. How does innate immune gene expression change throughout Amphimedon’s lifecycle?
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Ancient, abundant, invisible life forms
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Microbial-metazoan interactions•Marine bacteria - freeliving
- pathogenic - symbiotic
• Bacteria 3.5 bya• Sponges 650 mya
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Amphimedon queenslandica
• Heron Island• Indo-Pacific distribution• Hermaphroditic
spermcast spawner• Broods embryos
• First Poriferan genome Srivastava et al. 2010
• Alpha - proteobacteria bacteria sequenced
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Questions
1. How does Amphimedon acquire its symbionts?
2. How does the bacterial community of Amphimedon change throughout its lifecycle?
3. How does innate immune gene expression change throughout Amphimedon’s lifecycle?
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Microbial -metazoan interactionsSymbiont transmission
• Vertical - Poribacteria• Horizontal
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Questions
1. How does Amphimedon acquire its symbionts?
2. How does the bacterial community of Amphimedon change throughout its lifecycle?
3. How does innate immune gene expression change throughout Amphimedon’s lifecycle?
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Microbial communities
PC larvae Post larvae Adult FSW AdultShark Bay Seawater
PC larvae
Post larvae
Adult
FSW Adult
Least similar
Most similar
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Questions
1. How does Amphimedon acquire its symbionts?
2. How does the bacterial community of Amphimedon change throughout its lifecycle?
3. How does innate immune gene expression change throughout Amphimedon’s lifecycle?
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Innate Immunity: A first line recognition system
Vertebrates•Innate and Adaptive•Memory cells
Invertebrates•Innate•Alternative adaptive ?
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Innate Immunity: A first line recognition system
•Pattern Recognition Receptors
•Microbial Associated Molecular Patterns
Gram positive Gram negative
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Gene family MAMPs detected Gene(s) in Amphimedon
Homologues
Vertebrates Invertebrates
LBL LPS: G –ve bacteria 3
NLR PGN: G+ve and -ve 2
C-lectin Carbohydrates 1
Amphimedon innate immune genes of interest
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Amphimedon innate immune gene expression
Embryo Larvae Post larvae Adult0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
LBL
Gene
Exp
ress
ion
Embryo Larvae Post larvae Adult0
0.1
0.2
0.3
0.4
0.5
C-lectin
Gene
Exp
ress
ion
Embryo Larvae Post larvae Adult0
1
2
3
4
5
6
7
NLR
Gene
Exp
ress
ion
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Conclusions•Symbiotic microbes acquired via vertical and horizontal transmission•Symbiotic from transitory community•Immune genes induced or developmental?•Benthos•Feeding
Thank you...
•Sandie Degnan•Bernie Degnan(School of Biological Sciences, UQ)•Phil Hugenholtz (Australian Centre of Ecogenomics)•Degnan lab
http://web.me.com/degnanlab1/DegnanLab/Home.html
http://ecogenomic.org/users/jo-bayes