4 Indian JAnim Sci

Vol. 87 No. 4

pp 401-532

New '-'elhi

April 7.017

Price : ? 200.00 per copy

636 (6) CODEN : IJLAA4 87 (4) : 401-532 (2017) II ISSN 0367-8318

For online submission and open access visithttp://epubs.icar.org.in/ejournal

The Indian Journalof

Animal Sciences

INDIAN COUNCIL OF AGRICULTURAL RESEARCHNEW DELHI

/'.v

TT*''

w-

Vol. 87, No. 4

THE INDIAN JOURNAL OF

ANIMAL SCIENCESPrevious Issue : Vol. 87, No. 3, pp. 257—400

CONTENTS

ANIMAL HEALTH

April 2017

SliBSCR/3£[)

Review Article

Climate change and buffalo farming in major milk producing states of India - Present statusand need for addressing concernsA K Balhara, Varij Nayan, A Dey, K P Singh, S S Daliiya and Inderjeet Singh

Trichinellosis: An under recognised zoonosis in IndiaH B Chethan Kumar, Himani Dhanze, K N Bhilegaonkar and Ashok Kumar

Peste-des-petits-ruminants in goats: Sero-epidemiological study in middle Indo-Gangetic plainsPankaj Kumar, Bidya Shankar Sinha, Rama Krishna Roy, Rashmi Rekha Kumari and Abhay Kumar

Parasitologicai and molecular detection of bovine babesiosis in endemic areas of Kamataka state, South IndiaK U Vijayakumar, G C Puttalakshmamma, Placid E D'souza, P P Sengupta, B M Chandranaik

and C Renukaprasad

Kiss 1 and GPR54 mRNA expression, endocrine profile, follicular development and onset of estrusfollowing kisspeptin administration in pre-pubertal mithun heifersM H Khan, S B Hazarika, P Perumal, K K Baruah, T Yasotha and Papiya Sinha

Short communications

Impact of weaning age on post-partum reproductive performance and stress level in Murrah buffaloesP K Bharti, Triveni Dutt, B H M Patel, H O Pandey, G K Gaur, Mukesh Singh, Reena Kamal,K K Venna and Deepak Upadhyay

Effect of membrane stabilizer on the freezability of buck semenR Ranjan, R Priyadharsini, A K Gael, Brejendra Singh, Satish Kumar, S D Kharche and S K Jindal

ANIMAL PRODUCTION

Demographic parameters and disposal pattern in Sahiwal cattle HerdVipin Kumar Upadhyay, R K Mehla, A K Gupta and M Bhakat

Evaluation of 24 microsatellite markers for parentage exclusion in three indigenous pig types of IndiaRahui Belli, Jyotsna Behl, M S Tantia, N Nahardeka, G C Das, K Sajecv Kumar and R K Vijh

Phenotypic characterization of Rajapalayam dog of southern IndiaK N Raja, P K Singh, A K Mishra, I Ganguly, P Devendran and S Kathir\>el

Effect of feeding hydroponics maize fodder replacing maize of concentrate rnixture partially ondigestibility of nutrients and milk production in lactating cowsP K Naik, B D Dhawaskar, D D Fatarpekar. M Karunakaran, R B Dhuri, B K Swam,E B Chakurkar and N P Singh . j. „

Effect of vitamin E supplementation on semen and blood profile of vaccinate cross re u sT K S Rao, T K Mahanty and M Bhakat •

Effect of different non-antibiotic feed additives either alone or in combination on theperformance and cost economics of broiler chickensGurram Srinivas. V Chinni Preetam and B Swathi t- , • . , .

Growth performance, abdominal fat and fat digestibility in broiler chicken fed with syntheticemulsifier and natural biosurfactant

T.S Cheah, T C Hah, H Akit and 5 Kimkoolin Ovo nutrient supplementation improves the performance and intestinal morphology in early

feed deprived egg type chickensAnd S Shinde. Manish Mehra, J J Rokade, Akshat Gael, A B Mandal and S K Bhanja

Effect of zinc oxide nanoparticles on some antioxidant biomarkers and enzymes of broiler chickensduring starter pha.seF Ahmadi. Y Ebrahimnezhad, N Maheri-Sis and J Ghiasi Ghaleh-Kandi

403

412

418

422

427

432

435

437

443

447

452

456

461

464

473

480

Seasonal variation in fatty acid profile in the milk of different species under popularly followed feedingsystem in India

Saroj. B A Malla, L V Tran. A N Shanna. Sacliin Kumar and A K TyagiProcess optimization for the production of ghee residue protein hydrolysates

V Munirathnamma, Vijay Kumar Gupta and Ganga Sahay MeenaMilking management practices and IMI in Jersey crossbred cows in changing scenario

Champak Bhakat, A Chatterjee, D K Mancial, M Karunakaran, A Mandal, S Garai and T K DuttaOrganizational performance and management constraints of sheep and wool producers

co-operatives in KarnatakaH Dileep Kumar, Riipasi Tiwari. Triveni Dutt, K Pachaiyappan and B L Balaraju

Variables predicting the role performance of field extension functionaries in Animal Husbandry sectorof Karnataka; A multivariate approach

Diksha Patel, MCA Devi. S P Lai. A K Singh. A P Verma and 5 Smitha

484

490

495

501

506

Karnataka

Sampling Plan

Karnataka4

BangaloreBangaloreUrban

DAH&VS KMFI i

i JDAH&VS KMF

i i

)SRS

Major findings

Overall Role Performance

6 2.50' i>

7.50' h

Scree Plot

Plot of Eigen Value

EFA Analysis

Thunderbolt indicatingthe point of inflexionon the curve with 5

Line drawn throughsmaller eigen value

Low Medium High

1 2 345 6789 1011 121314151617Factor number

Relationship between independent variable and role performance;Multivariate analysis

Total respondents = 120PS-Purposive SamplingSRS-Simple Random Sampling, EPFs- FieldExtension Functionories

Significant

Marital 'it itus, Family occupation,Attitude towards work. Jobinvolvorienl, Job statisfaction.Information seeking behaviour

NonsignificantDesignation, Age, Educationalqualification. Work experience.Training received, Organisationalclimate

Dependent variable

Role

performance of FEFs

Cause * Effect

(Significantrelationship)

Cause ► Effect(Nonsignificantrelationship)

PATRICIA trie based time and memory optimization for fast network motif Search 512Himanshu. K K Chaturvedi. A Bandyopadhyay and Sarika Jain

Short communications

Evaluation of non-genetic factors aftecting lactation traits of Fricswal cows in northern zone of India 520Rani Alex. Sushil Kumar. Umesh Singh. Rajih Deb. Rafeecfue R Alyethodi. B Prakash and Gurudev Singh

Performance of Ghungroo and Ghungroo x Hampshire pigs under low input production system in 523climatic condition of Garo hills of Meghalaya

S Borah. T Samajdar. P K Bluirti. T Kumar Da.s and G MurakTetra-primer ARMS-PCR assay for genotyping SNPs in ovine GDFH gene associated with mutton 525

traits in sheepReena Arora, I) K Yadav. Anjii .Shanna, Mountka Polhuraju. Neeiika Ti/z/vrt/r and Yashila Girdhar

(ienctic polymorphism of bone morphogenetic protein receptor type-1 (BMPRIB) gene in 529crossbred docs of BiharRqjni Kumari. Shanker Daval. Sanjay Kumar. P C ('handran and A Dey

Vol. 87, No. 5

CONTENTS

SUBSCRIBED

THE INDIAN JOURNAL OF

ANIMAL SCIENCESPrevious Issue : Vol. 87, No. 4, pp. 401-532

May 2017

Review Article

Role of infectious disease information system and risk assessment in control of livestock diseases inIndian perspectives: A review

K P Suresh, S S Patil, L Yasaswini, D Hemadh, G S Desai and H Rahman

ANIMAL HEALTH

Distribution of serogroup specific antibodies against leptospirosis in livestock in OdishaV Balamurugan, S Veena, S R A Thirunialesh, A Alamim, R Sridevi, P P Sengiipta, G Govindaraj,M NagaUngam, D Hemadh, M R Gajeiidragad and H Rahman

Prevalence and haemato-biochemical changes of tick-borne haemoparasitic diseases in crossbredcattle of Haryana, India

Anita Ganguly, R S Bisla, Harpreet Singh, Vandna Bhanot, Ankit Kumar, Sonu Kumari,Biswa Ranjan Maharana and Indrajit Ganguly

Seasonal prevalence of gastrointestinal parasites of goats in North -East Himalayan region of Sikkim, IndiaPapri Pal, L R Chatlod and R K Avasthe

Isolation and characterization of Salmonella bacteriophages from poulty and pig sewage wastesTarun Kumar, V S Rajora, Niddhi Arora, Anand Prakash and S K Shiikla

Worm control approaches and their impact on status of anthelmintic resistance at an organized sheep farmD Singh and C P Swarnkar

Prediction of buffalo bull fertility based on sperm motion traits, function tests and expression ofheparin binding protein (HBP)A K Singh, P S Brar, Ranjna S Cheema and Pradeep Kumar

Short communications

Prevalence of various diseases and health disorders in Murrah buffaloes at organized herd of hotsub-humid eco-region

Narendra Pratap Singh, Triveni Dull, P K Bharti, Mukesh Singh, G K Gaur and B H M PatelVirulence markers and antimicrobial resistance of Streptococcus suis isolated from diseased pigs

S Rajkhowa, D K Sarma and S R PeguBreeding soundness evaluation of stall-fed bucks using ultrasonography

M Singh, A L Saini, Ajeet Kumar, S Kaswan and P S Brar

536

546

552

558

562

568

573

579

581

584

Total

sperms/ Live Rump fatejaculae sperms thickness

Buck scrotum

Normal

testes

Ultrasound

scan

Mildfibrosis

\Extensive

fibrosis

Quantitative and qualitative assessment of seminal parameters of Manipuri breed stallions reared in arid" n Rao Tallari. S K Ravi. A.shok Kama,- Chaudha,y. .1S Mehia. R A Legha.

Yash Pal. A K Gupta and B N Tripathi

587

ANIMAL PRODUCTION

Identification of SNP variations and different microRNA binding sites at 3'UTR region of bovine 590HSP90AA1 gene among different Indian breeds of cattle

Rajib Deb, Gyanendm S Sengar Basavraj Sajjaiiar, Umesh Singh, Indra Singh, Rani Alex, T V Raja,Sushi! Kumar, R R Aiyethodi and V Bhanuprakash

Hazra chicken: A precious germplasm in need of immediate scientific intervention 597Rekha Shanna, Hiinani Shanna, Sonika Ahlawat, N Barik, P K Singh and M S Tantia

Impact of feeding essential oils on feed fermentation and rumen miciobial profile in crossbred cattle calves 604A Kumar, D N Kamra, N Agarwal and L C Chaudhary

Feeding pattern, metabolic status and milk composition of Chilika buffaloes in their natural habitat 610S Mohanty, N Panda, B Panigrahi, R K Swain, S K Dash, A Mishra and S S Giri

Effect of n-3 polyunsaturated fatty acid rich fish oil supplementation on serum biochemical profile in goat 619A K Verma, A 5 Mahla, R K Chaudhari, S K Singh, A K Singh, K Narayanan and Harendra Kumar

Palm oil rich diet

(Control group; CG)

-■ix

FetillBC riik/paln Oil

t r

APBSPC

t TPG) M

AccliRutizaiion periodHlnc4l umpllnyrrousglandin ii\joction

N=20 (5 goats/group)

n-3 PUFA rich fish oil diet(T1 =78 mg/kg)(T2=156 mg/kg)(T3=312mg/kg)

a 0.0

3 0.0

rr 016

CD 0 08-E

(0 0 3-7 20 36 55Day of supplementation

« 0 041

• CG » T1 » T2 -TO

-7 20 38 55Day of supplementation

•CG * T1 •T2-T3

-7 20 38 55Day of supplementation

Higher thedose of

n-3 PUFA,lower

the serumcholesterol

Influence of biologically active substances from Kombucha (Medusomyces gisevii) on rat gut microbiotawith experimental antibiotic-associated dysbiosis

Bondareva Nadezhda Ivanovna, Timchenko Lyudmila Dmiirievna. Dobrynxa Yuliva Mihailo <Alieva Elena Vasil'evna, Rzhepakovsky Igor' Vladimirovich, Likhacheva Elena Sergeevm""

Sizonenko Marina Nikolaevna. Piskov Scrgeyivanovich, Kozlova Maria and Areshid'e £David

Control group I

Gentamycinsulfate

Experimentalgroup I

Dysbiosis

■S •Control group II

Bacteriologicalresearch of thelarge intestinemicroflora

Experimentalgroup II

Dysbiosis

#

#Bacteriologicalresearch of thelarge intestinemicroflora

Biologically Restorationactive substance of the intestinalfrom Medusomyces rnicrofloragisevii

m

Prevention of dysbiosis

#

624

Effect of maternal dietary manipulation and in ovo injection of nutrients on the body weight gain,feed conversion ratio, development of lymphoid and digestive organs of turkey poults

Ainitav Bliatfacharyya, Saniir Majwndar. Siibrat Kumar Blianja, Asit Baraii Mandal andMukiind M Kadam

Association between Mspl calpastatin gene polymorphisms, growth performance and meatcharacteristics of Awassi sheep

Khaleel IJawasreh, Rola Jadallah, A H AI-Amareen, Abdullah Y Abdullah, Alaa Al-Qaisi,Ibrahim M, AlRawashdeh, Moh"D Borhan F Al-Zghoul, M Khair Ahamed and Belal Obeidat

Effects of com husks derived xylooligosaccharides on performance of broiler chickenA K Samanta, A P Kolte, A V Elangovan, A Dhali, S Senani, Manpal Sridhar and Natasha Jayapal

Effect of feeding different level of Moringa oleifera leaf meal on growth performance, lipid profileand meat fatty acid composition of Vanaraja chicken in tropics

Abhishek Kumar, Kaushalendra Kumar, Sanjay Kumar, Chandramoni, RRK Sinha,J K Faswan and G P Mandal

Contribution of livestock in livelihood: Ladakli regionAfzal Hoque Akand, B P Singh and Mahesh Chander

630

635

640

644

649

awardshousehold,income

Less feedsand fodder

Milk basednutritional/ \security / + + +

Inaccessibleextension

Nutnentsto the ' ^farmsReduced

pasture

Employmengeneration/ ̂

Winterstress

ecurity foruncertaintie

High pricedeeds & fbdde The realistic location specific approach

Efforts by multiple agencies to addressdifferent drawbacks and convert livestock

to a lucrative enterprise.

Statussymbol/ x

Short communications

Practical methods of gender identification in Kashmir geeseHenna Hamadani, Azmat Alam Khan, Ambreen Hamadani and Andleeb Rafic/

CoiTclation between in vitro activity of milk leukocytes with milk constituents in high yielding

Buragohain, Sarath Chandra Reddy, Pradip Kumar Das, Probal Ranjan Ghosh.Dipak Banerjee and Joydip Mukherjee h

^ ..p. ■ r n w..r.in rp-^idues in broiler chicken tissues using competitive enzyme-linkedQuantification ol enronoxacin lesioiiesimmunosorbern assay , j pirees, Q U Nisa, R Akhtar and G SalecmM Younus, T Abbas, M Zafar, S Ra^a, A i^nan.

653

656

659

SUBSCRBED

Vol. 87, No. 6

THE INDIAN JOURNAL OF

ANIMAL SCIENCESPrevious Issue : Vol. 87, No. 5, pp. 531-664

June 2017

CONTENTS

Review Article

Apolipoproteins in fish: From lipid transport to innate immunity 668P K Sahoo, Amruta Moluipalrci and J K Jena

ANIMAL HEALTH

Serological indication of exposure to foot-and-mouth disease virus in comprehensively surveyed 680traditionally managed small ruminant population of Gujarat plains and hills agro-climatic region of India

Manoranjan Roiil, Bikash Ranjan Priisty and Amil KananiComparative evaluation of FAT, IS90P PCR and microscopy vis a vis histo-pathology for the 685

detection of Mycobacterium aviiim subsp paraliiberciilosis infection in tissues of goats naturallydied in herds endemic for Johne's disea.se

Shivam Chaliirvedi, Slioor Vir Singh, Ashok Kumar Srivaslava, Neeraj Kumar Gangwar,Naveen Kumar, Krishna Dutta Rawat, Saurabh Gupta, Kundan Kumar Chauhey,

Rajendra Singh, Raghavendra Singh and Kuideep DhamaAntioxidant potential of coenzyme QIC in Escherichia coli associated calf diarrhea 694

J Garkhal, G E Chethan, V K Gupta, S Qureshi, R Mukherjee, U Dimri, G K Gain;R K Aganval and U K De

Comparative evaluation of isoflurane and .sevoflurane for maintenance of anaesthesia in buffaloes 701undergoing diaphragmatic herniorrhaphyR N Chaudharv, Rishi Taval, S M Behl. Ashok Kumar and Satbir Sharma

Divided into two

groups of six animaleach for maintenance

IFT

W

i Administered. . .':iglycopyrrolate|'Anaesthesiar j! ^ wasinducedRumenotomy ̂ Xylazine ^jth

-jTiopenJorjeButorphanol -

12 buffaloes

with DH

Maintained

with

isoflurane

Maintainedwith

sevoflurane

Maintained on agent specificvaporizer during surgery

•Recoverytook more

time

•Respirationwas more

depressed•Alteration ofdepth slow

•Fasterrecovery

•Respirationwas less

depressed•Alteration ofdepth faster

Sevoflurane is

better

maintenance

agent than

isoflurane for

buffaloes

Bio-stimulatory effects of bull urine (BU). PRID and GnRH on hormonal profile and association withblood minerals in pubertal anestrus Sahiwal heilers

Ahrar Ahmed. R P Tiwari, J R Khan. S P Tmari and G K MishraAntioxidant and antimicrobial activity of protein hydrolysate extracted from porcine liver

Akhilesh K Verma. Manish Kumar Chal/i. Pavan Kumar and Nitin MehtaBovine fluorosis and its effects on essential mincials. hacmogiam and biochemical status in the fluoride

endemic South-West Punjab of IndiaS T Singh. K Dua. D K Gupta. SS Randhan a and B K Bansal

707

711

718

Survey on fluorosisand its effects onbovine tiealttn

^ Fluorotic^ Animals

Mansa Buffalo: 95.3Cattle ; 100.0

Buffalo : 92.0

Cattle :95.7

35.6

Fazllka 9.9

District Prevelance of Water fluoride

flourosis >1.5 ppm(Plasma F >0.1 ppm) (%)

+ plasma phosphorus!plasma zinc♦ plasma manganese ♦ plasma molybdenum+ plasma ALKP ♦PCVandTEC

• Region : South \west Punjab, India• Districts : Mansa, Fazilka•Animals : Buffalo and Cattle• Sampling ; Stratified random sampling

Seasonal changes in hematological and biochemical profile of dairy cows in high altitude cold desertArup Giri, Vijciy K Bharti, S Kalia, V Ravindran, P Ranjan

723

Short communications

Cryopreservation of mithun semen; comparative study of conventional vs controlled freeziu"M H Khan, Papiya Sinha, P Perumal and S B Hazarikat Raj Kiindan and B Kumar

728

ANIMAL PRODUCTION

Microsatellite based polymorphism and genetic diversity among Indian buffalo breedsBheemashankar H Kammr, Md. Nadeem Fairoze, P S Girish. C Renukaprasad. Nagappa Karahasanavar,Shailesh Bagale and B H Rudresh

Phenotypic, genetic and environmental trends of production traits in Phule Triveni synthetic cowG S Ambhore, Avtar Singh, D K Deokar, Manvendra Singh and S K Sahao

Influence of drinking structured water on production performance, nutrient retention, carcass characteristicsand economics in commercial broiler chicken

P Vasanthakwnar, D Chandrasekaran, M R Purushothaman, C Kathirvelan, S R Janani andS Senthilkiimar

Effect of herbal feed supplement on milk yield and composition in crossbred cows in TripuraTapan Kumar Das, Bikas Chandra Debnalh, BiJoy Kumar Sarkar. Ankan De and Shivi Maini

Maize silage as sole forage source for dairy cows m small-scale sy.stems in the highlands of central MexicoA SJaimez-Garcia, D Heredia-Nava, J G Estrada-Flores, F Vicente, A Martinez-FernZTF Lopez-Gonzalez and C M Arriaga-Jorddn

Assessment of microclimate and gaseous pollutants in dairy and pig sheds in an organised farmD Divyalakshmi, N Kumaravelu, B Samuel Masilamoni Ronald, S Meenakshi \undm-nm nnHThanga Thamil Vanan ' "

Effect of parity, stage of lactation and udder type on milkability of crossbred dairy cows milked inautomated Herringbone milking parlour

A Fahim. M L Kamboj. S Prasad, A S Sirohi, M Bhakal, TK Molianty and R Malholra

731

736

742

747

752

757_.,

761

Cow number

Neck transponderElectronic animal

Identification system

Herd managementprogramme

Ir\i IISystem controller

4 Recording of milkabjlltyparameters of Individual cowsCrossbred cows In higher

parity and early stage of lactationperformed best

Herringbone milking parlour(8x2)

Short communications

BMP! gene is a direct target gene of miR-378 in cattleTeng-He Ma

Single nucleotide polymorphism (SNP) analysis of ovine progesterone receptor gene in Malpura ewesRajiv Kumar, S K Sankhyan, A K Singh and B Krishnappa

Fold changes in relative mRNA expression of immune response genes in lymphoid tissues of RhodeIsland Red chicken

Abdul Rahim, Sanjeev Kumar. Jowel Debnath, Ramji Yadav. Ananla Kumar Das and A S YadavUse of body surface area in rural buffaloes

S B Gokhale, T V Shirsaih. D R Sonawane and D N ShindeNew breeding objectives for the Czech pig population

Z Krupovd, E -cikovd, E Krupa and M Michaiiekovd

768

770

772

775

778

Is It still OK ?

and what about: ?

• health

• feed efficiency

• other...

Select pig on:

Dam breed Sire breed

V growth intensity V growth intensity\ litter size \ carcass quality

V carcass quality

+ new: + new:

feed efficiency number feed efficiencyof teat sperm quality

Selection of pig until now:

\ growth

V reproductionV carcass

Characterizing grower performance, body conformation and morphology in crosses of RIR and Indiannative chicken genotypes

Ananla Kumar Das, Anil Kumar Mishra, Sanjeev Kumar, Abdul Rahim and Laxmikant Sambhaji KokateGenetic and non-genetic parameters of grower and layer performances in CARl-Sonali chicken

Ananla Kumar Das, Sanjeev Kumar. Abdul Rahim and LaxmikanI Sambhaji KokaleUtilization of Blepharis sindica herbage in sheep feeding: Effect on nutrient utilization, rumen fermentation

and. plane of nutritionR S Bhall, A Sahoo, Lalil Kumar Soni and S K Sankhyan

782

786

790

SUBSCRIBED

Vol. 87, No. 7

THE INDIAN JOURNAL OF

ANIMAL SCIENCESPrevious Issue : Vol. 87, No. 6, pp. 665-796

July 2017

CONTENTS

Review Article

Present status and future prospects of Deoni cattleV B Dortgre, R S Gandhi, V MSalunke, L S Kokate, S MDurge, V N Khandait and P V Patil

800

ANIMAL HEALTH

Calcium-dependent and calcium-mimicking pathways regulate lead-induced myometrial contractionin water buffaloes

Udayraj P Nakade, Abhishek Sharma. Soumen Choudhury, Shirish Bhatiya, SooraJ VNair,Vipin Sharma, Rajkumar Singh Yadav and Satish Kumar Garg

804

Non-pregnant water buffalo Uterus collected from

slaughter house

Minrcs Ca

To activate PKC

\

/Facilitate entryof Ca'"

Uterine strips mounted inorgan bath

Buffalo myometrial contraction

Ovarian diseases in Holstein dairy cows, factors determining their occunence and impact onreproduction traits and breeding costsJ Citek, D NemeckovA, L Stadnik and R Stiipka

Molecular characterization and in-silico analysis of myeloid cathelicidin gene in Swamp buffaloD J Kalita, S Sharma and A Barua

810

814

Collection ofbone marrow_myeloid ceilsand extraction

of RNA

Reverse transcriptionwith specific

designed primerand cloning andsequencing of

amplified PGR product

.a.Agarose gel

electrophoresisof PGR and

cloned cDNA

Macrophagephagocytosis f

Nucleotide and predictedamino acid sequenceof cathelicidin cDNA of ~

bone marrow myeloidtissues of swamp buffalo

Designing andprediction of mostpotent AlVlP frombuffalo myeloid ''cathelicidin

Antimicrobial t

effect I

Antigen-specific ♦' immune |

responses

Efficacy of progesterone implants on induction of cyclicity in anestrous buffaloesA Jerome, S K Srivastava, R K Sharma and 5" K Phulia

Follicular dynamics, homional and biochemical profile across seasons in buffaloesA Jerome , S K Srivastava, R K Sharma, S K Sarkar and R Kumat

Morphometric analysis of mithun sperm from fresh and frozen-thawed semenM H Khan, Papiya Sinha, P Perumal and S B Hazarika

819

824

829

The semen sampleafter dilution was

cooled gradually from35°C to S-C and thenfrozen In biofreezerunder controlledfreezing rate

Both fresh and frozen-

thawed semen werestained with Eosin-

Nigrosln and giemsa stainand digitized for

morphometric evaluation

Mithun bulls semensample were subjected for

cryopreservation withcommercial extender

Optixel

Schematic representation of sperm morphometry in fresh and frozen Mithun semen

Preparation of bioclimatograph for haemonchosis and tricliostrongylosis in goats of subtropical high humid 834zone of Sikkim

Paph Pal. L R Chatlod and R K Avasthe

Short communications

Levamisole as an immunomodulator to ameliorate vaccination stress in crossbred bulls 840

T K S Rao, T K Mohanty and M BhakatIn-vitro antibacterial potential of aqueous and ethanolic extract of Aegle marmelos and Murraya 844

koenigii leaf on the bacterial strains isolated from endometritic cowsRupali Rautela, G K Das. Shiv Prasad. Avadhesh Kumar. J K Prasad. S K Ghosh and Naveen Kumar

Cow sufferingwith endometritis

Efficacv of extract■ Ethanolic > Aqueous (in combination)• Ethanolic > Aqueous (alone)

Evaluation of extract againstbacteria isolated

Antibacterial effect ofleaves extract

Ethanolic and aqueousextract of Aeg/e marmelos

and Murraya koenigii

Oxidative and haemato-biochemical alterations in theileriosis affected cattle from semi arid endemicareas of India

Vijay Pandey. Rajesh Nigam. Ram Bachan. Vikrant Sudan. Am it Kumar Jaiswal. Dava Shankar.Rahul Kumar. Rajesh Mandil and Brijesh Yadav

Goat semen cryopreservation using egg yolk and soya based extenders containing trehaloseB M Narwade. Tushar Kumar Mohantw Mukesh Bhakat and Abdul Rahim

Effect of different farming systems on physiological response, blood parameters and endocrinologicalprofiles in Deccani sheepSA Kochewad.TRaghimandan. K Sarjan Rao. K Kondal Reddy. N Nalini Kumari. D BVRamana.

T C Balamurgan. Yogeshwar Kankarne. Sanjeev Kumar and L R Meena

T1, Intensive farming system; T2,

Comparative T3, T4 and cortisol levels

Semi-intensive farming system; T3, Extensive farming systemDecanni sheep

50-

40.

30-

20

10

0.

m

HiV-| r

■

T3 T4 Cortisol

(nmol/L) (nmol/L) (nmol/L)

Blood parameters and liver function test

50

5 og. §CO CL^ 8

Physiological response

*1h* ̂

h- o ̂05

oB& CO w </) c:.

Respirationrate

(Morning)

Respirationrate

(Evening)

Pulse rate

(Morning)Pulse rate(Evening)

IT1

IT2

IT3

Rectal

temperature(Morning)

Rectal

temperature(Evening)

animal production

ElTect of DGATI. FASN and PRL genes on milk production and milk composition traits in Simmentaland crossbred Holstein cattle

\1aja Maurice . Tomislav Masek. Miroslav Benice, Marija Spehar and Kristina Stareevice

846

851

856

859

Multivariate analysis of biometric traits and their shared variance in Chhattisgarhi buffaloVikas Vohra, Mohan Singh, Ramendra Das, Alka Chopra and R S Kataria

Genetic variability and phylogenetic relationship establishes distinctness of Kaunayen chicken of ManipurRekha Sharma, Priyanka Sharma, Sonika Ahlawat, T H Ranadhir Singh, P KVij, R K Vijh and M S Tantia

864

871

Conservation of Indigenous livestock is important

Kaunayen Ctiickenof ilianipur, India

Genetic diversityanalysis

-Ankaleshwar-Busra

AseelDanki

— Gliagus

Kalatiasttii

MegtialayaDaothiigiriri

Kasti FavTeliictierryKadaknattiNicobari

Pbrown

PGR amplification using25 microsateilite markers

Kaunayen groups separately fromregistered Indian poultry breeds

Genotyping usingABi 3130XL sequencer

-Red Jungle Fowl-Kaunayen

"Wtiite Leg Horn

-Hill Fowl

-H Black

0.71 -

0.69-

Registeredas 17th breed of

India

0.64-

^bservalioh

I

IObserved

heterozygosityExpected

heterozygosity

Heterozygote deficiency

Feed and fodder availability in Punjab state vis- a- vis livestock population -An estimateP S Tanwar and H K Verma

Amelioration of adverse effects of heat stress using custom design shelter system and supplementingchromium propionate to growing Karan Fries calves during summer

Sunil Singh, S V Singh, Renuka, Yogendra Kumar, And Kumar, Beenam Baliyan and S S KunduMicrobial protein estimation in Murrah buffalo calves fed on diets with varying levels of energy and protein

Sonali Prusty, S S Kundu and Papori TalukdarSupplemental effect of different levels of energy and protein along with groundnut straw

{Arachis hypogaea L.) based ration on nutrient utilization m dromedary camelsLokesh Gupta, A K Roy and G S Tiwari

Effect of some phytogenic additives as dietary supplements on performance, egg quality, serumbiochemical parameters and oxidative status in laying hens

Mahmoud Alagawany Mohamed E Abd El-Hack, Muhammad Saeed, Muhammad A Arain,

Zohaib A Bhutto, Sarfraz A Fazlani, Sarfraz A Brohi and Muhammad Arif

879

885

891

896

900

Thyme powder

ncrease

Egg production and quality

IgG \\ IgM C ., ■>

Rosemary powderHi-sex

Brown layinghens

Lipid profileand

antioxidant capacity

Push and pull factors of migration amongst livestock rearers distressed by national calamityin India: A Polytomous Universal Model analysisSudhanandPrasadLaJ. KSKadianmA Workneh Abebe Wodajo

Impact of environmental factors on milk constituent and yield traits i.i Murrah buffaloesA Upadhyav, A K Chakravart)'. A K Gupta, A S Selvan and A P Singh

Effect of housing systems on fatty acid structures of liver, abdominal fat and breast muscle in broiler productionI Iku Gulcihan Simsek. Okkes Yilmaz. Zehra Gokce. Mehmet Cijtci and Zeki Erisir

Short communication

Polymorphism in the SREBP-I gene is associated with milk production traits of Jersey cattleU itoid StanisiAw Proskura, Andrzej Dybus, Daniel Zaborski. Zbigniew Sobek. Yu-hsiang Yu and

Yeong-hsiang Cheng

906

912

919

924

SIIBSCRf3ED

Vol. 87, No. 8

THE INDIAN JOURNAL OF

ANIMAL SCIENCESPrevious Issue : Vol. 87, No. 7, pp. 797-928

CONTENTS

ANIMAL HEALTH

Comparative intensity of infection and performance of iambs selected for resistance or susceptibilityto Haemonchits contortus in semi-arid RajasthanC P Swarnkar, D Singh and L L L Prince

TALEN construction for porcine iKBa gene and the detection of knockout activityKui Xu, Jinxue Ruan, Hegang Li, Tianwen Wu, Bo Liang and Yulian Mii

August 2017

932

939

For Xenotransplantation

1

TALEN construction for pig kBa gene

Pig IkBo. gene Exont

TGGATCACCGCCACGACAGCGGCCTGGACrccATGAAGnArfiflrinftnTflrf;^

TALEN-L TALEN-R

SSA detect the knockout activity

termination codon target fragment

Basic skeleton

of SSA piasmid \ /the arms of ludferase

I TALEN

1homologous recombination —^=1:^of left and right arm j

ludferase appear —sam mmt—•positive signal

Transfection of early and late stage sheep spermatogonial stem cells in cultureF Jasoui: MZandi, K Hoseini Pajooh, MR Sanjabi and H Ofoghi

An analysis of matrix metalloproteinases (MMPs) activity in excretory/secretory antigens ofOestrus avis larvae from sheep

S Arunkumar, R Prakash Krupakaran and P PerumalPCR-based sexing of caprine embryos using SiylAinl-X duplex PCR and 5n'/DVEPC041 duplex PCR

Ramli Bin Abdullah, Choon Yen Alice Phua, Mohammad Mijanur Rahman andWan Embong Wan Khadijah

Pregnancy diagnosis in pigs using seed germination inhibition test: An evaluation study in MeghalayaAshok Kumar, R K Dewry, Ram Singh, P C Jat, Maluik Singh and G Kadin>el

Fertility response in post-partum buffaloes following bypass nutrient supplementationG S Katiyar, R K Sharma, V Mudgal, A Jerome, S K Phulia and I Singh

Effect of seasonal variation on physiological parameters in Murrah buffaloesShanker Dayal. Amitava Dey. S J Pandian.././ Gupta, P C Chandran and Imran Ali

Short communications

In vitro anthelmintic activity of Syzygium aromaticum and Melia dubia against Haemonchus contortus of sheepV Gnani Charitha J Adeppa and P Malakondaiah

Standardisation of loop mediated isothermal amplification assay for rapid detection of New Duck diseaseSiuya Sunilkumar, Uma Radhakrishnan and Shonima Pala

Molecular characterization and phylogenetic sequence analysis of unique conserved portion ofVSG of Tiypanosoma evansi

Anjali Devi. Daya Shanker Vikrant Sudan. Amit .Jai.iwal and Amit Singh

944

947

951

956

960

965

968

971

974

Molecular characterization and sequence phylogenetic analysis of Babesia bigemim cattle isolatefrom Mathura based onl8S ribosomal DNA gene

]^ih-an1 Sudan, Daya Shankar, Barkha Sharma. Amit Kumar Jaiswal and Amit SinghB1 gene based semi nested PCR for detection of toxoplasmosis from poultry hearts

Anjali Devi, Vikrant Sudan, Amit Jaiswal, Amit Singh and Daya Shanker

977

980

ANIMAL PRODUCTION

Estimation of genetic diversity and relationship among goats of Maharashtra statePriyanka Mishra, S P Dixit, RA K Aggarwal. Jyotsna Behl, P S Dangi and N K Verma

Identification of best performer Hilly chicken's of Bangladesh in consideration of climate change factors:light and heatMKIKhan, A MA MZSiddiki, MR Ali and MA Akter

982

991

Hilly area

Hilly male

Hilly female

Desi male

Reddish brown

Spotted white

White

- ¥Black

Desi female Grey

Treatment

groups

Control

Egg production of chicken based on various treatment

Heat stress

Light

¥ ̂ 50-r^ 11

^6co

t33•oo

O)O)Hi

.8 78.96 81

50 -r '

Control HeatStress

Treatment

I Hilly IND

Replacement rate and its components in Sahiwal females from birth to age at first calvingVipin Kumar Upadhyay, R K Mehla, A K Gupta. M Bhakat, S S Lalhwal and S K Yadav

Effect of paddy straw based complete rations with different levels of neutral detergent fibre on microbialprotein synthesis in dairy cows during early and mid lactation

Bijii Chacko, Syam K M Mohan. K Ally, K Shyama, K S Anil and C TSalhianEffect of grasshopper meal on laying hens' performance and eggs quality characteristics

Notiri Brah, Salissou Issa and Frederic M Houndonougbo

996

1000

1005

-t

GlOO

Evaluation of

laying hens'

performance

and egg quality

characteristics

Daily feed intake andlaying rate were similar

(P>0.05) in all dietary

treatment.

Replacement of FM with

GM did not significantlyaffect feed efficacy and

efficiency (P>0.05).

In Niger, poultry

farmers could

replace fish meal by

grasshopper meal up

to 100% In layers diet

GM significantly improved

egg yolk colour and Haugh

unit, but did not affect other

egg quality parameters. j-

GO. G25. Ci50 (i75 and GlOO: Diets that substituted FM with GM at 0%. 25%. 50%. 75% and 100%, respectively. FM. Fish meal; GM,Grasshopper incal.

Utilization ol enzyme incubated rape seed meal as a source of protein in commercial broiler dietsS y Roma Rao. M V L N Rajii, B Prakash, S K Verma. U Rajkumar and E P K Reddy

1011

Current status of goat husbandry and scope for improving its productivity in Bihar, Odisha and Uttar PradeshNarayan G Hegde and Avinash D Deo

1018

Service bystraybucks

Diseases/

mortalityMiddlemen

for sellingFeed shortageParasites

Low income

? 1,000Traditional Goat Husbandry

Goat keepers with small flocksImproved Goat Husbandry

Service byelite bucks

Higherincome

? 10,000Vaccinations Deworming Fodder/

FeedFair trade

Competitiveness of Indian bovine meat exports ~ constant market share analysis 1026K Varalakshmi and Sttresh Devatkal

Use of social media for livestock advisory services: The case of WhatsApp in Himachal Pradesh, India 1034Devesh Thakur and Mahesh Chander

Short communications

Estimation of factors for standardizing lactation yield to Mature Equivalent basis and factors affecting 1038305 day mature equivalent milk yield in Frieswal cattle

SushiI Kumar, Rani Alex, Uinesh Singh, T V Raja, Rajib Deb, Rafeeque R Alyethodi and B PrakashGenetics of body weights in a control line of Rhode Island Red grower chicken 1042

Ananta Kumar Das, Sanjeev Kumar, Anil Kumar Mishra, Abdul Rahim and Laxmikant Sambhaji KokateEffect of feeding compressed complete feed block with or without deoiled mahua seed cake on methane 1046

production and energy utilization in crossbred calvesA K Patil, V B Chatimvedi, Putan Singh, A K Verma and Ash Das

Vol. 87, No. 11

THE INDIAN JOURNAL OF

ANIMAL SCIENCESPrevious Issue : Vol. 87, No. 10, pp. 1173-1292

November 2017

CONTENTS

ANIMAL HEALTH

Exploration of genetic basis of differential immune response to CSF vaccination in desi (indigenous)piglets using RNA-Seq approach

Vaishali Sail, Amit Kumar, Ravi Kumar. Shalu Kumari Patliak, Sajad Ahmad Warn,Amit Ranjan Sahii. Vikramaditya Upmanyu. Nihar Ranjan Salioo and Bharat Bhushan

Immuno-reactivity of recombinant non-structural protein N-terminus (rNSNt) in indirect-ELISA fordetection of bluetongue viral antibodies in serum samplesN Chacko S K Biswas. N N Mohanry, K Charid. B Mondal. A B Pandey and S B Shivachandra

Cvtokine nene expression and pathology in experimental Pasteurella midtocida infection in micePKlishnamoorthy.Sangita Das. BR Shame ̂ ndParimal RoyExpression of Echinococcus gramdosus recombinant EgAg- sub-unit and P- antigens and evaluation of

their diagnostic potential for cystic echinococcosis in buffaloesMary Nisha Tigga. Ajayta Rialch. A Arun. S Samanta. Navneet Kaur. R K Bauri. Z A Ganaie.

Aiidlceb Aftab and O K RainaMolecular expression and characterization of GCP gene of Haemonchus contortus

M Aravind. B C Saravartan, H V Manjunathachar. M Saiikar. R Saravanan. A Prasad.AAP Milton and G Bhuvana Priya

1297

1304

1310

1314

1320

rMarker

Amplification ofGCP7 by PGR

Cloning of GCP7 intopTZ57R/T vector

Sub cloning intopET32a(+)

expression vector

Protein expressionand purification

Western blotting ofexpressed protein

H. contortusparasites in sheep

In vitro antioxidative potential of Artemisia indica Willdp.iKhna Ruwali, Tanuj Kumar Amhwani and Pankaj Gautam

Evaluation of bio-engineered comeal scaffold for the repair of comeal defect in ra it mo eP Sangeetha. S K Maiti. Kiranjeet Singh. Aswathy Gopinathan, K P Singh , ivya o la ,

A R Ninu and Naveen Kumar . • u • hullCryoprotective effect of low-density lipoproteins on post thaw ZT^a

MukulAnand, Sarvajeet Yadav, Vijay Singh. Shalini Vaswani and Pankaj K Shukla

1326

1332

1340

V.A

Collection of semenfrom Hariana bull

(n=4)LDL incorporation insemen extender

WT,,

TS Cryopreservationand evaluationafter 7 days

T4

-

Assessment of semen

quality duringcryopreservation

Incorporation of 9% LDLin semen extender

has better capacity tomaintain the sperm

character in post thawHariana bull semen

Control

(20% EY) SUBSCRIBED

Developmental competence of goat oocytes vitrified at immature and mature stage in comparison tofresh oocytes after in vitro fertilization using cauda epididymal spermatozoa

Dimple Kumari, Dharmemlra Kumar, Sandeep Rahangdaie, Lalit Mohan Jeena, Ajit Pratap Singh andBikash Chandra Sarkhel

1345

Follicular oocytes aspiration from slaughterhouse goat ovaries

Soild surface vitrification and thawing of immature oocytes ■ Immature goat oocytesj

In vitro maturation

Mature oocytes Mature oocytes

Group 1Group 2

Solid surface vitrification and

thawing of mature oocytes

Group 3

In vitro fertilization of different oocytes groups from slaughtered epididymal buck

2-4 cells 8-16 cells!Developmentalcompetence Morula Blastocyst

Factors influencing production and reproductive performance of Frieswal cattle maintained at organizedfarm conditions

S K Rathee, A K Gupta, T V Raja and A K Chakravarty

Short communication

Detection and characterization of caprine and ovine rotaviruses, India

Uttam Singh, Rashmi Singh, Ajay Pratap Singh, Sharad Kumar Yadav, Shubhankar Sircar andYashpal Singh Malik

1350

1358

r- rA

• €

Collection of ovine (n=100) and caprine (n=125) feecal samples

SvQ

II

Sandwich ELISA

for group A rotavirus

1 caprine sample waspositive while all

ovine samples were negative

I

\ ^

iK: pwti N

-tiRNA-PAGE Electro-pherotyping

IAli sample were negative forRV genome in RNA-PAGE

RT-PCR assay10 caprine (Prevalence=8%) and

5 ovine (Prevalence=5%)samples were positive

VP-7(G) & VP-4 (P) basedmultiplex nested PGR genotyping

Single caprine possessed G6G8P[11]genotype combination while rest of the

samples remained untypeable

ANIMAL PRODUCTION

Characterization of genetic polymorphisms in Toll-like receptor gene of Bos indicus Sahiwal cattleJyotsna Dhingra Belli, Anurodh Shama, R S Kataria, N K Venna, Shiv Prasad Kimothi,

Avnish Kumar Bhatia and Rahul Behl

1362

■■fi

V.f

Forward primer

Bos indicus Sahiwal Toll-likereceptor9 gene

Reverse primerPGR gene amplification

Sequencing andanalysis of

amplified genesequence for SNPs

Eleven SNPs (8 in coding region and 3in non-coding region) within 4.8 kb regionofTLR 9 gene

SNPs 2930 (G to A) caused amino acidchange G437E in the TLR9 protein

These SNPs led to generation of 12genotypes and 11 most probablehaplotypesInformation on SNPs shall be helpful inimproving the resolution of geneticmarkers across the TLR 9 gene for thepurpose of mapping for associationanalysis for disease resistance traitobsen/ed in Sehiwal animals

Genomic variations in the oligoadenylate synthetase (OAS) gene in zebu cattle and its crossbreds ofIndian origin

Rani Alex, K P Ramesha. Umesh Singh, Sushil Kumar, Rafeeqiie R Alyethodi, Rajib Deb,Sheetal Sharma, Gyanendra S Sengar, Asliish and B Prakash

Expression pattern of Rheb gene in Jabal Barez Red goatAzra Najmi Noori, Mohammad Reza Bahreini Behzadi and Mohammad Reza Mohammadabadi

Genetic variability in egg production-associated microsatellites in Rhode Island Red chickenJowel Debnath, Sanjeev Kumar, Abdul Rahim and Ramji Yadav

Beneficial effects of increasing dietary levels of raw lentil seeds on meat fatty acid and plasma metabolicprofile in broiler chickens

Georgela Ciurescu, Andreea Vasilachi, Mariana Ropotd, Mihai Palade and Catalin DragomirFatty acids profile of breast muscle (%]

1367

1375

1379

1385

One-day-old

28 dayfeeding trial

cobb 500 broilerchickens (n=1000)

"Corn/soybeanmeal diet (control diet)

• 200g/kg of raw lentilseeds cv. Eston (LE diet)

• 400g/kg of LE200g/kg of raw lentilseeds, cv. Anicia (LA diet)

. 400g/kg of LA

Assessment offatty acid profile

Measurementof blood plasma

parameters

l.r190 11

lINTrl

Meat FAs profile wasaffected by treatment.Breast muscle of broilerfed lentil seeds hadsignificant higher levelof alfa-llnolenic, FPA,DPAand DMA.

.1 .1

Raw lentil seedsis an alternative proteinsource which can improvethe quality of broiler meatand can be recommendedIn healthy k>alanced diets.

Blood plasma parameters(i.e. cholesterol, totalprotein, albumin,totalglobulin, creatinine. urea,Ca. P, Mg, Fe.ALT/GPT, AST/GOT. alkalinephosphatase, GGT, andLDH) was not affected bytreatments, except forglucose and triglycerides.

Anti.Kidant and antimicrobial activity of ultra-filtered fraction-s of camel milk protein hydrolysates underin vilro condition

Devendra Kumar. Manish Kumar Chatli, Raghvendar Singh, Nitin Mehta and Pavan KumarFactnrs affecting milk quality of crossbred dairy cows in automated Herringbone milking sy.stem

\ Fahim, M L Kainboj. A S Sirohi. M Bhakat and T K MohantyPeiiiTinance of cros.sbred cattle (HF x Sahiwal) under tropical farming conditions of Punjab

Ravinder Kumar A K Das. T P Raja. S K Rathee, P P Dubey and B Prakash

1391

1396

1402

Lifetime performance of Phule Triveni synthetic cows at an organized farmG S Ambhore. Avtar Singh, D K Deokar, Manvendra Singh and S K Sahoo

Constraints in livestock service delivery through mobile veterinary units (MVUs): Evidence from OdishaAnupama Jena, Mahesh Chander and D Bardhan

Short communications

Phenotypic characteristics and general managemental practices for working donkey populations inSouth Western Bihar Region of IndiaA K Gupta, Sanjay Kumar, Yash Pal, Mamta Chauhan, B Kumar and Prince

Effect of different nutiient solutions on the biomass yield and nutritive value of low cost hydroponicfodder maize

S Gunasekaran, C Bandeswaran and C Valli

1406

1410

1414

1418

Maize seed Soaked In water for 16 h

5■D•5 4-^

18^I 2-

CO0

Kept for sprouting in agunny bag for 32 h

Hydroponic fodder cultivationusing different nutrient solutions

'S

Control 10% 0.1%(water) vermiwasti urea

10% 2.5%diluted biogas

cow urine slurry

Higtiest biomass yield was observedwith water as a nutrient solution