THE HYPERSPECTRAL IMAGING APPROACH
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Transcript of THE HYPERSPECTRAL IMAGING APPROACH
THE HYPERSPECTRAL IMAGING APPROACH
•Giuseppe Bonifazi
•Workshop
ACTIONS C.1&C.2 - Process and product monitoringbased on hyperspectral imaging
HyperSpectral Imaging (HSI) is an innovative technology developed in recent years that combines the advantages of spectroscopy and imaging techniques.
In the last years there was a strong increase in applications including off-line / on-line inspection at industrial scale in different sectors.
Image source: www.perception-park.com
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
All individual spatial and spectral images could be picked up from the hypercube and the spectrum of each pixel of the image in a specific position can be extracted
One spectral image of n pixels for all the investigated wavelengths (λ)
Spectral signature of a single pixel
ACTIONS C.1&C.2 - Process and product monitoringbased on hyperspectral imaging
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Hyperspectral Imaging Characterization
SISUChema XL™ Chemical Imaging Workstation
• Operation mode:push-broom• Spectral range:
SWIR: 1000-2500nm• Spectral sampling and
(resolution)6.3nm• Active pixel:320 (spatial) x 240 (spectral)
pixels
ACTIONS C.1&C.2 – The selected HSI devices
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
ACTIONS C.1&C.2 – The selected HSI devices
Hyperspectral Imaging Characterization
Hyperspectral architecture Specim ImSpectorTM N17E
• Operation mode:push-broom• Spectral range:
NIR: 1000-1700nm• Spectral sampling and
(resolution)5nm• Active pixel:320 (spatial) x 240 (spectral)
pixels
Hyperspectral architecture Specim ImSpectorTM V10
• Operation mode:push-broom• Spectral range:
VIS-NIR: 400-1000 nm• Spectral sampling and
(resolution)5 nm• Active pixel:320 (spatial) x 240 (spectral)
pixels
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
ACTION C.1: HyperSpectral Imaging (HSI) Characterization
Actions C.1-C.2 - Sample Acquisition
Console
Monitor
Moving belt
PC
Spectral-imaginginstrumentation
Illuminant
Console
Monitor
Moving belt
PC
Spectral-imaginginstrumentation
Illuminant
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Actions C.1-C.2: Hyperspectral data processing
Spectral data have been analysed using the PLS_Toolbox 7. 0 (Eigenvector Research Inc.) running inside Matlab™ environment (version 7.12.0).
•Spectra preprocessing
•PCA applied for data exploration
•PLS applied for spectra correlation with curing time and different measured chemical parameters
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Actions C.1&C.2 – Results on raw materials from the different countries (Action B.1)
Step 1: Waste raw materials collected from Italy, Spain and Cyprus were utilized to train the HSI system to correctly classify them.
Step 2: Hyperspectral images of different organic waste were elaborated using the 3 different HSI devices.
Step 3: Spectra preprocessing and PCA classification model were applied for the classification of different organic matrices.
CY-UOW Sample IT-FOR Sample
1 cm
Applied pretreatment:- SNV- Mean Center
b)
a)
c)
a)
b)
c)
Raw Hyperspectal images of a) IT-BC; b) IT-FOR and c)
IT-UOW
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
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1000 1050 1100 1150 1200 1250 1300 1350 1400 1450 1500 1550 1600 1650
Refle
ctan
ce
Wavelength[nm]
IT-UOW_001_N17 IT-UOW_002_N17 IT-UOW_003_N17 IT-UOW_004_N17
IT-FOR_001_N17 IT-FOR_002_N17 IT-FOR_003_N17 IT-FOR_004_N17
IT-BC_001_N17 IT-BC_002_N17 IT-BC_003_N17 IT-BC_004_N17
IT-BC_V2_001_N17 IT-BC_V2_002_N17 IT-BC_V2_003_N17 IT-BC_V2_004_N17
IT_FOR Sub-sample
IT_BC Sub-sample
IT_UOW Sub-sample
IT_BC_V2 Sub-sample
Raw materials: Italian samples Reflectance spectra (1000-1700 nm)
Actions C.1&C.2 – Results on raw materials from the different countries (Action B.1)
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Pretreated Hyperspectal images of a) IT-BC; b) IT-FOR and c) IT-UOW
a) b) c)
a) IT-BC
b) IT-FOR
c) IT-UOW
a) b) c)
Raw Hyperspectal images of a) IT-BC; b) IT-FOR and c) IT-UOW
Applied pretreatment:- SNV- Mean Center
b)
a)
c)
Actions C.1&C.2 – Results on raw materials from the different countries (Action B.1)
Italian Raw Materials
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Pretreatment applied: SNV + Mean Center
CY –FOR_V2
ES-FOR
IT-FOR
PCA score plots (PC1 vs. PC2) related to the different FOR samples coming from Italy, Spain and Cyprus acquired in the NIR (1000-1650 nm) wavelength field.
Actions C.1&C.2 – Results on raw materials from the different countries (Action B.1)
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Italian samples at different curing times
Average reflectance spectra of the different Italian samples acquired in the NIR (1000-1650 nm) wavelength field
Actions C.1&C.2 – Results from lab-scale plantsin the different countries (Action B.2)
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Italian samples at different curing times
PCA score plots (PC1 vs. PC2) related to the different Italian samples (IT-UFVB-5050-T0; IT-UFVB-5050-T20; IT-UFVB-5050-T40 and IT-UFVB-5050-T60) acquired in the NIR (1000-1650 nm) wavelength
field
Actions C.1&C.2 – Results from lab-scale plantsin the different countries (Action B.2)
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
t = 0 days
t = 20 days
t = 40 days
t = 60 days
Process Monitoring by HSIPLS for different curing times (Italian samples)
Actions C.1&C.2 – Results from lab-scale plantsin the different countries (Action B.2)
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Product Monitoring by HSIPrediction of chemical parameters (Italian samples)
Actions C.1&C.2 – Results from lab-scale plantsin the different countries (Action B.2)
Humidity (%) Phosphatase (µmol INTF g-1 h-1)
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Product Monitoring by HSIPrediction of chemical parameters (Italian samples)
Actions C.1&C.2 – Results from lab-scale plantsin the different countries (Action B.2)
Soluble Organic Nitrogen (ppm) Soluble Organic Carbon (%)
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Product Monitoring by HSIPCA (Spanish samples)
Actions C.1&C.2 – Results from pilot-scale plantsin the different countries (Action B.3)
Sample
Background
Source SampleES-UFVB-6040 T90
Sample Hypercube PCA: background removal
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Process Monitoring by HSIRaw and preprocessed spectra at t = 0 and t = 90 days (Spanish samples)
Actions C.1&C.2 – Results from pilot-scale plantsin the different countries (Action B.3)
Preprocessing applied: Smoothing (window 11)+ Second derivative (window 15)+ Mean center
Average raw spectra
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Process Monitoring by HSIPCA and Loadings (Spanish samples)
Actions C.1&C.2 – Results from pilot-scale plantsin the different countries (Action B.3)
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Actions C.1&C.2 – Results from pilot-scale plantsin the different countries (Action B.3)
Product Monitoring by HSIPrediction of chemical parameters: Soluble Organic Nitrogen (%)
Italian samples Spanish samples
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Actions C.1&C.2 – Results from pilot-scale plantsin the different countries (Action B.3)
Product Monitoring by HSIPrediction of chemical parameters: Soluble Organic Carbon (%)
Italian samples Spanish samples
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Actions C.1&C.2 – Results from pilot-scale plantsin the different countries (Action B.3)
Product Monitoring by HSIPrediction of chemical parameters: EC (mS/cm)
Italian samples Spanish samples
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
IT-BA-L1-T1-i IT-BA-L1-T2-i IT-BA-L1-T6-i IT-BA-L1-T1-f IT-BA-L1-T2-f IT-BA-L1-T6-f
Actions C.3 – Results from agriculture application in Italy (Action B.4)
Product Monitoring by HSIPCA (Italian samples)
- Barley
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Actions C.3 – Results from agriculture application in Italy (Action B.4)
Process Monitoring by HSIRaw and preprocessed spectra
Preprocessing applied: Smoothing (window 15)+ First derivative (window 15)+ Mean center
Average raw spectra
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Actions C.3 – Results from agriculture application in Italy (Action B.4)
PCA score plots (PC1 vs. PC2) related to Barley samples acquired in the NIR (1000-1650 nm) wavelength field.
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Actions C.4 – Results from agriculture application in Spain (Action B.5)
ES-PO-L1-T1-i ES-PO-L1-T2-i ES-PO-L1-T6-i ES-PO-L1-T1-f ES-PO-L1-T2-f ES-PO-L1-T6-f
b)
a)
Product Monitoring by HSIPCA (Spanish samples)
- Potato
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Actions C.4 – Results from agriculture application in Spain (Action B.5)
Process Monitoring by HSIRaw and preprocessed spectra
Preprocessing applied: Smoothing (window 15)+ Second derivative (window 15)+ Mean center
Average raw spectra
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Actions C.4 – Results from agriculture application in Spain (Action B.5)
PCA score plots (PC1 vs. PC2) related to Potato samples acquired in the NIR (1000-1650 nm) wavelength field.
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Actions C.5 – Results from agriculture application in Cyprus (Action B.6)
CY-ME-L1-T1-i CY-ME-L1-T2-i CY-ME-L1-T6-i CY-ME-L1-T1-f CY-ME-L1-T2-f CY-ME-L1-T6-f
a)
b)
- Melon
Product Monitoring by HSIPCA (Cypriot samples)
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Actions C.5 – Results from agriculture application in Cyprus (Action B.6)
Preprocessing applied: Smoothing (window 11)+ Second derivative (window 19)+ Mean center
Average raw spectra
Process Monitoring by HSIRaw and preprocessed spectra
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Actions C.5 – Results from agriculture application in Cyprus (Action B.6)
PCA score plots (PC1 vs. PC2) related to Potato samples acquired in the NIR (1000-1650 nm) wavelength field.
Conclusions
Actions B.1&C.1-C.2The HSI approach allowed to outline the difference existing among the investigated raw material samples both in terms of composition (i.e. UOW, FOR and BC) and origin (i.e.: Italy, Spain and Cyprus), with the only exception of Italian and Spanish BC.
Actions B.2&C.1-C.2Good correlation between spectral behavior in the NIR wavelength range of the different collected samples from the lab-scale plants in the different countries and curing time and the main measured chemical parameters (humidity, soluble carbon, soluble nitrogen, and phosphatase) were obtained.
Actions B.3&C.1-C.2Good correlation between spectral behavior in the NIR wavelength range of the different collected samples from the pilot-scale plants in the different countries and the main measured chemical parameters (soluble carbon, soluble nitrogen and electrical conductivity) were obtained.
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Conclusions
RESAFE ProjectWorkshopFaenza (Italy)December 14, 2015
Actions B.4&C.3The HSI approach allowed to outline the difference crops existing from different thesis in the agriculture application in Italy. Actions B.5&C.4The HSI approach allowed to outline the difference crops existing from different thesis in the agriculture application in Spain.
Actions B.6&C.5The HSI approach allowed to outline the difference crops existing from different thesis in the agriculture application in Cyprus.