The future of paper chromatography in the wine industry ...The future of paper chromatography in the...
Transcript of The future of paper chromatography in the wine industry ...The future of paper chromatography in the...
The future of paper chromatography in the wine industry due to alternative methods
Carien Coetzee
SAWLA Workshop26 May 2017
Paper chromatography
Accuvin™Vinmetrica SC-
50
Enzymatic Spectrometric
Analysis
FTIRWinescan
HPLC
LOQ
Time for analyses
Hazards
Cost per sample
Instrument cost
Ease of use
Availability
Simpler methods Complex methods
Kit contains• Wide mouth jar• Capillary pipettes• Chromatography papers• Chromatography solvent
• Tartaric acid standard• Malic acid standard• Lactic acid standard
Paper
chromatography
Paper
chromatography
• Small sample volume• Limited training required• Readily available• Low cost• Kit only
• 24 hours• Hazardous chemicals• Not quantitative• Interpretation subjective
Malic acid
Lactic acid
Paper
chromatography
Kit contains• Sampler• Test strip• Colour chart
Accuvin™
Accuvin™
• Small sample volume• Limited training required• NOT Readily available ?• Low-medium cost
• Quantitative• 4 minutes• No Hazardous chemicals• Interpretation subjective• Kit only
• Vinmetrica SC-55 Manometer • Malic Reagent set• Biopressure Agent Reaction Vials• 1 ‘check’ vial • 10 ml serological pipette• 15 ml conical tube• Plastic bulb pipette• 100 mL polypropylene beaker• Plastic Scoop
• Distilled water• Microwave oven
Vinmetrica
SC-50
Vinmetrica
SC-50
• Small sample volume• Limited training required (more than previous)• Not Readily available ?• Low-medium cost
• Quantitative• 30-60 minutes• No Hazardous chemicals• Need to acquire instrumentation + kit
• Spectrophotometer • Cuvettes • Micropipettes • Enzyme test kit (includes buffer solution, NAD,
enzymes, standards)
Enzymatic
Spectrometric
Analysis
Enzymatic
Spectrometric
Analysis
• Small sample volume• Laboratory training required• Readily available• Low-medium cost per sample
• Quantitative• 30 minutes• No Hazardous chemicals• Need to acquire instrumentation + kit
Measure absorbance using a spectrophometer
Enzymatic
Spectrometric
Analysis
• Auto analyzers are convenient, but costly to purchase✓ Eliminates analyst variation✓ Less labour intensive ✓ Small volume needed✓ Capacity for increased sample numbers ✓ Decrease in cost per sample
• Skilled personnel • Periodic maintenance
• Foss Winescan / OenoFoss
FTIR
Winescan
FTIR
Winescan
• Winescan/OenoFoss is convenient, but costly to purchase✓ Eliminates analyst variation✓ Less labour intensive – very easy to use✓ Small volume needed✓ Capacity for increased sample numbers ✓ Decrease in cost per sample✓ Automization available
• Skilled personnel • Periodic maintenance• Constant checks due to matrix effects
• Small sample volume• Laboratory training required• Low-medium cost per sample
• Quantitative• 1 minute• No chemicals• Need to acquire instrumentation
• HPLC, column and assorted equipment • Sample vials and caps • Filtration membranes and syringes • Acetonitrile, phosphoric acid, and distilled water • Filtration unit for reagents
HPLC
HPLC
• Skilled personnel • Periodic maintenance
• Small sample volume• Laboratory training required• High cost per sample
• Quantitative• 40 minutes• Potentially hazardous chemicals• Need to acquire expensive
instrumentation
Paper chromatography
Accuvin™Vinmetrica SC-
50
Enzymatic Spectrometric
Analysis
FTIRWinescan/OenoFoss
HPLC
Qualitative Semi-Quantitative Quantitative Quantitative Quantitative Quantitative
LOQ n/a 0.03 g/L 0.1 g/L 0.05 g/L 0.001-0.05 g/L
Time for analyses
24 hours 4 min 30-60 min 30 min 1 min 40 min
Hazardschemicals noxious,
flamablenone irritant mildly corrosive none
chemicals flamable, corrosive, irritant
Cost per sample
R7 R40-R50 R40-R50R320 (after instrument)
R650 (after instrument)
Instrument cost
kit kit R2 000 R45 000 R1 200 000 R1 200 000
Ease of use * * ** *** *** ****
Availability **** * * *** *** ***
Paper
chromatographyAccuvin™
Vinmetrica
SC-50
Enzymatic
Spectrometric
Analysis
FTIR
WinescanHPLC
The future of paper chromatography in the wine industry due to alternative methods
✓ Easy to perform✓ Readily available✓ Low cost
• Useful for wineries: in-house checks• Can then confirm with accredited laboratory the completion of MLF• Not feasible in routine laboratories
Not Quantitative Does it need to be?
SAWLA Workshop26 May 2017