The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel
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Transcript of The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel
![Page 1: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/1.jpg)
The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing
Temperature of the Gel
![Page 2: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/2.jpg)
Samples(55:31:14)
Sample Name ExperimentalSample Name
Percentage
Hitenol+Noigen(Ratio Hit:Noi)
Butyl Methacrylate Water
A W 54.7%(12.9:1)
30.6% 14.7%
B 1 54.6%(10.1:1)
31.5% 13.8%
C 3 55.3%(9.6:1)
30.4% 14.3%
D Z 54.4%(8:1)
31.3% 14.3%
E 2 55.2%(7.5:1)
30.9% 13.9%
![Page 3: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/3.jpg)
Samples(55:31:14)
Sample Name
Percentage
Hitenol+Noigen(Ratio Hit:Noi)
Butyl Methacrylate Water
A54.7%
(12.9:1)30.6% 14.7%
B54.6%
(10.1:1)31.5% 13.8%
C55.3%(9.6:1)
30.4% 14.3%
D54.4%(8:1)
31.3% 14.3%
E55.2%(7.5:1)
30.9% 13.9%
![Page 4: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/4.jpg)
Samples(55:31:14)
Sample Name
Percentage
Hitenol+Noigen(Ratio Hit:Noi)
Butyl Methacrylate Water
A54.7%
(12.9:1)30.6% 14.7%
B54.6%
(10.1:1)31.5% 13.8%
C55.3%(9.6:1)
30.4% 14.3%
D54.4%(8:1)
31.3% 14.3%
E55.2%(7.5:1)
30.9% 13.9%
![Page 5: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/5.jpg)
70 75 80 85 90 950
0.1
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0.7
0.8
0.9
1
Temperature (C)
Inte
nsity
Normalized Intensity vs Temperature for 2 heat-cool cycles
Heating Cycle 1Cooling Cycle 1Heating Cycle 2Cooling Cycle 2
Sample A - 12.9:1
![Page 6: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/6.jpg)
70 75 80 85 90 950
0.1
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0.5
0.6
0.7
0.8
0.9
1
Temperature
Nor
mal
ized
Inte
nsity
Sigmoidal Fit of 2nd Heating & Cooling Curves
Sigmoidal FitCooling Cycle DataSigmoidal FitHeating Cycle Data
The value of the (cooling) clearing temperature is: 87.4812The value of the (heating) clearing temperature is: 86.5312
Sample A - 12.9:1
![Page 7: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/7.jpg)
Samples(55:31:14)
Sample Name
Percentage
Hitenol+Noigen(Ratio Hit:Noi)
Butyl Methacrylate Water
A54.7%
(12.9:1)30.6% 14.7%
B54.6%
(10.1:1)31.5% 13.8%
C55.3%(9.6:1)
30.4% 14.3%
D54.4%(8:1)
31.3% 14.3%
E55.2%(7.5:1)
30.9% 13.9%
![Page 8: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/8.jpg)
74 76 78 80 82 84 86 88 90 92 940
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
Temperature (C)
Inte
nsity
Normalized Intensity vs Temperature for 2 heat-cool cycles
Heating Cycle 1Cooling Cycle 1Heating Cycle 2Cooling Cycle 2
Sample B - 10.1:1
![Page 9: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/9.jpg)
Sample B - 10.1:1
74 76 78 80 82 84 86 88 90 92 940
0.1
0.2
0.3
0.4
0.5
0.6
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0.8
0.9
1
Temperature
Nor
mal
ized
Inte
nsity
Sigmoidal Fit of 2nd Heating & Cooling Curves
Sigmoidal FitCooling Cycle DataSigmoidal FitHeating Cycle Data
The value of the (heating) clearing temperature is: 86.5326The value of the (cooling) clearing temperature is: 84.5354
![Page 10: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/10.jpg)
Samples(55:31:14)
Sample Name
Percentage
Hitenol+Noigen(Ratio Hit:Noi)
Butyl Methacrylate Water
A54.7%
(12.9:1)30.6% 14.7%
B54.6%
(10.1:1)31.5% 13.8%
C55.3%(9.6:1)
30.4% 14.3%
D54.4%(8:1)
31.3% 14.3%
E55.2%(7.5:1)
30.9% 13.9%
![Page 11: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/11.jpg)
70 75 80 85 90 950
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
Temperature (C)
Inte
nsity
Normalized Intensity vs Temperature for 2 heat-cool cycles
Heating Cycle 1Cooling Cycle 1Heating Cycle 2Cooling Cycle 2
Sample C - 9.6:1
![Page 12: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/12.jpg)
70 75 80 85 90 950
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
Temperature
Nor
mal
ized
Inte
nsity
Sigmoidal Fit of 2nd Heating & Cooling Curves
Sigmoidal FitCooling Cycle DataSigmoidal FitHeating Cycle Data
The value of the (heating) clearing temperature is: 85.7208The value of the (cooling) clearing temperature is: 82.4773
Sample C - 9.6:1
![Page 13: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/13.jpg)
Samples(55:31:14)
Sample Name
Percentage
Hitenol+Noigen(Ratio Hit:Noi)
Butyl Methacrylate Water
A54.7%
(12.9:1)30.6% 14.7%
B54.6%
(10.1:1)31.5% 13.8%
C55.3%(9.6:1)
30.4% 14.3%
D54.4%(8:1)
31.3% 14.3%
E55.2%(7.5:1)
30.9% 13.9%
![Page 14: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/14.jpg)
60 65 70 75 80 85 900
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
Temperature (C)
Inte
nsity
Normalized Intensity vs Temperature for 2 heat-cool cycles
Heating Cycle 1Cooling Cycle 1Heating Cycle 2Cooling Cycle 2
Sample D - 8:1
![Page 15: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/15.jpg)
60 65 70 75 80 85 900
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
Temperature
Nor
mal
ized
Inte
nsity
Sigmoidal Fit of 2nd Heating & Cooling Curves
Sigmoidal FitCooling Cycle DataSigmoidal FitHeating Cycle Data
Sample D - 8:1
The value of the (heating) clearing temperature is: 80.1485The value of the (cooling) clearing temperature is: 74.0822
![Page 16: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/16.jpg)
Samples(55:31:14)
Sample Name
Percentage
Hitenol+Noigen(Ratio Hit:Noi)
Butyl Methacrylate Water
A54.7%
(12.9:1)30.6% 14.7%
B54.6%
(10.1:1)31.5% 13.8%
C55.3%(9.6:1)
30.4% 14.3%
D54.4%(8:1)
31.3% 14.3%
E55.2%(7.5:1)
30.9% 13.9%
![Page 17: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/17.jpg)
60 65 70 75 80 85 900
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
Temperature (C)
Inte
nsity
Normalized Intensity vs Temperature for 2 heat-cool cycles
Heating Cycle 1Cooling Cycle 1Heating Cycle 2Cooling Cycle 2
Sample E - 7.5:1
![Page 18: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/18.jpg)
60 65 70 75 80 85 900
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
Temperature
Nor
mal
ized
Inte
nsity
Sigmoidal Fit of 2nd Heating & Cooling Curves
Sigmoidal FitCooling Cycle DataSigmoidal FitHeating Cycle Data
The value of the (heating) clearing temperature is: 75.299The value of the (cooling) clearing temperature is: 73.2607
Sample E - 7.5:1
![Page 19: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/19.jpg)
5 6 7 8 9 10 11 12 13 1465
70
75
80
85
90
Graph Showing Effect of Hit:Noi Ratio on Clearing Temperature
Clearing temp (heating curves)Clearing temp (cooling curves)
Hit/Noi ratio
Clea
ring
Tem
pera
ture
![Page 20: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/20.jpg)
How concentration affects NIR results
![Page 21: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/21.jpg)
7,000 8,000 9,000 10,000 11,0000.0
0.2
0.4
0.6
0.8
1.0 measured spectrum simulated spectrum basis function
Nor
mal
ized
em
issi
on in
tens
ity
Optical frequency (cm-1)
Fluorescence Spectrum638 nm excitation
7/28/2011 10:29:42E:\99C:\Users\Taneja\Documents\OriginLab\85\User Files\ilution_startingfrom10mgopermlCNT_original.opj - [Fluor638]
AppliedNanoFluorescence
(Original )CNT dispersed in 1% surfactant solution, sonicated at 30 W for 15 minutes,
centrifuged at 6000rpm for 30 minutes, decanted and process diluted to ~ 99% and
then process repeated
![Page 22: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/22.jpg)
7,000 8,000 9,000 10,000 11,0000.0
0.2
0.4
0.6
0.8
1.0 measured spectrum simulated spectrum basis function
Nor
mal
ized
em
issi
on in
tens
ity
Optical frequency (cm-1)
Fluorescence Spectrum638 nm excitation
7/28/2011 10:21:06E:\99C:\Users\Taneja\Documents\OriginLab\85\User Files\ilution_startingfrom10mgopermlCNT_original_halfdiluted.opj - [Fluor638]
AppliedNanoFluorescence Diluted to 50% of Original Concentration
![Page 23: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/23.jpg)
7,000 8,000 9,000 10,000 11,0000.0
0.2
0.4
0.6
0.8
measured spectrum simulated spectrum basis function
Nor
mal
ized
em
issi
on in
tens
ity
Optical frequency (cm-1)
Fluorescence Spectrum638 nm excitation
7/28/2011 10:17:19E:\99C:\Users\Taneja\Documents\OriginLab\85\User Files\ilution_startingfrom10mgopermlCNT_original_quarterdiluted.opj - [Fluor638]
AppliedNanoFluorescence Diluted to 25% of Original Concentration
![Page 24: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/24.jpg)
Observations
•General shape the same
•Intensities different-most dilute solution has overall lower intensity as expected-50% dilution shows increase in intensity at most optical frequencies but NOT at the frequency with the highest peak (???) […Saturation?]
•Smoothness different-smoothness of curve decreases as we move from highest to lowest concentration (possibly lower concentrations = increased sensitivity… or not?)
![Page 25: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/25.jpg)
How Power Affects dispersion of SWNT
![Page 26: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/26.jpg)
7,000 8,000 9,000 10,000 11,0000.0
0.2
0.4
0.6 measured spectrum simulated spectrum basis function
Nor
mal
ized
em
issi
on in
tens
ity
Optical frequency (cm-1)
Fluorescence Spectrum638 nm excitation
7/28/2011 11:08:58E:\99C:\Users\Taneja\Documents\OriginLab\85\User Files\ilution_startingfrom10mgopermlCNT_12WattSonication.opj - [Fluor638]
AppliedNanoFluorescence Sonication @ 12W for 15 minutes
![Page 27: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/27.jpg)
7,000 8,000 9,000 10,000 11,0000.0
0.2
0.4
0.6 measured spectrum simulated spectrum basis function
Nor
mal
ized
em
issi
on in
tens
ity
Optical frequency (cm-1)
Fluorescence Spectrum638 nm excitation
7/28/2011 11:09:55E:\99C:\Users\Taneja\Documents\OriginLab\85\User Files\ilution_startingfrom10mgopermlCNT_27WattSonication.opj - [Fluor638]
AppliedNanoFluorescence
Sonication @ 27W for 15 minutes
![Page 28: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/28.jpg)
Observations
•General shape the same
•Peaks more well-defined in higher-power sonication
![Page 29: The Effect of Varying the Ratio of Hitenol to Noigen on the Clearing Temperature of the Gel](https://reader035.fdocuments.net/reader035/viewer/2022062520/56816337550346895dd3c4f5/html5/thumbnails/29.jpg)
http://www.swentnano.com/tech/docs/Procedure_for_dispersing_SWNT.pdf