The Effect of Asparagus on Cancer Cell Growth
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Transcript of The Effect of Asparagus on Cancer Cell Growth
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Can Aspargaus inhibit population
growth of yeast cells?Tiffany Sato
Experium Science AcademyMarlborough School, Ninth Grade
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Hypothesis and Goals During digestion, asparagus produces sulfur
compounds, which is beneficial in the reduction of the growth of cancer cells (Mitchell, 2001). Therefore, I decided to investigate this further which led me to the information that asparagus produced high levels of sulfur. From this, I decided to start studying the effect of asparagus on yeast activity.
I wanted to see a decrease in yeast activity compared to a negative and positive control. A decrease in growth meant that the asparagus, the high amounts of sulfur, was inhibiting the growth of the yeast cells.
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Yeast Cells (Saccharomyces cerevisiae) Typical eukaryotic
structure
Fast and incontrollable cell division
Economically accessible
Genetic material is known
Cancer Cells Typical eukaryotic
structure
Fast and incontrollable cell division
Why I used Yeast Cells and the Similarities
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Yeast Cells as a Model Organism
I realized that scientific studies have shown that Saccharomyces cerevisiae could be used as an alternative to cancer cells when doing research.
(Hartwell, 2001; Mortimer, 1973)
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Research Methods
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Recipes
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Yeast Activity Growth with
Spectrophotometer
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Viable Colony Counts using Agar Plates
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Results for Yeast Growth Activity
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Absorbance of Yeast Activity Sample
0 2 4 6 8 10 120
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
POSITIVENEGATIVEEXPL
Sample Numbers
Ab
sorb
an
ce (
600n
m)
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Data Table
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Data
Average for Negative (without any nutrients) : 0.189 abs
Average for Experimental (with asparagus): 0.488 abs
Average for Positive (with nutrients, without asparagus):0.633 abs
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Conclusions for Yeast Growth Activity
My results from my experiment with the liquid medium with asparagus had the lowest absorbance (0.488 abs) compared to my positive control (0.633 abs ) and higher absorbance compared to my negative control (0.189 abs). This indicates a decrease in yeast activity upon addition of asparagus indicating minimal cell growth.
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Results for Viable Colonies
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Experimental Results
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Comparison of the Controls
Negative Experiment Positive Experiment
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Conclusions from Viable Colonies
Although viable colonies have limited quantification, the pictures show the apparent difference of structural colonies between the Positive, Experimental, and Negative.
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Acknowledgements
Thank you to Sigma Xi for this opportunity. Thank you to Raudhah
Rahman, Experium Science Academy, my parents, and those who have supported
me throughout.