The assessment of the effects of low- intensity laser radiation on cells of tumor strain of kidney...
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![Page 1: The assessment of the effects of low- intensity laser radiation on cells of tumor strain of kidney cancer PA-1 Viktoria V. Skvortsova, Grigory E. Brill,](https://reader035.fdocuments.net/reader035/viewer/2022070323/56649d9f5503460f94a8a3a1/html5/thumbnails/1.jpg)
The assessment of the effects of low-intensity laser radiation on cells of tumor strain of kidney cancer PA-1
Viktoria V. Skvortsova, Grigory E. Brill, Galina A. Afanasyeva,
Alla B.BucharskayaSaratov State Medical University, Russia
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PurposeThe purpose of the study was to
investigate the effects of low-intensity red laser in vitro on cells of tumor strain
of kidney cancer PA-1 and assess the tumor transplantability in rats after laser
irradiation, macro- and microscopic
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Materials and methods12 white outbred male
rats weighing 200 ± 20 g were used in the
experiments. The tumor strain of kidney cancer PA-1 was obtained in
Bank of tumor strains of Oncological Centre n.a.
N.N. Blokhin.
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Materials and methods
Immediately after removal of tumors from donor rat,
cancer tissue weighing 30 g was washed with
physiological saline, added to 10 ml of Hanks solution and mechanically milled.
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Materials and methods
Suspension of tumor cells (2.5 ml) was exposed to laser irradiation previously, using semiconductor red laser (λ - 660 nm, output power - 24 mW) in a continuous mode during 20 min.
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Materials and methods
• The suspension of tumor cells in a volume of 0.5 ml was administered to animals
subcutaneously in the midscapular region. Irradiated suspension was injected to six
rats from experimental group. • Six animals from control group received
non-irradiated tumor cell suspension.
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Methods• Observations of tumor
growth and the measurement of tumor volume was performed
for 21 days
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Results
Significant differences were marked on the 17th day of the experiment, when the
average tumor volume was 10,2 ± 2,8 cm3 in the experimental group, 2,26 ± 0,75 cm3
- in the control group (p<0,02).
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Dynamics of tumor growth
9 11 13 15 17 19 210
5
10
15
20
25
control gr.
experimental gr.
Days
Volume, cm3
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Results
On the 19th day of experiment the average tumor volume was 13,9 ± 1,6 cm3 in the experimental group, 4,03 ± 0,77 cm3
- in control (p<0,001).
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Results
Finally, on the 21st day the size of transplanted tumors was 23,0 ± 3,8 cm3 in the experimental group, 10,4 ± 2,2 cm3
- in control (p<0.02).
against…
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Histologically, we did reveal some differences in samples of tumors between
experimental and control groups.
experiment control
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We observed cells with degenerative changes, with increased eosinophilia of the cytoplasm, hyperchroma-tic and smaller nuclei, that occupy from 40 to 70% of the area. Lumen of blood vessels occur from single to 10 in sight. experiment
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Conclusion
The stimulation of tumor growth of kidney cancer PA-1
was recorded when suspension of tumor cells were previously
exposed with low-intensity laser irradiation in vitro.
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Some questions without answers…
• How does biological activity of kidney cancer cells modify under the influence of laser irradiation?
• Does laser change genetic apparatus of cancer cells?• How long and unambiguous is effect
of exposure at these characteristics of the laser?
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So, still a lot remains unclear in the tumor response to irradiation by low-power laser, which requires a special approach - immunogistochemical techniques, spectroflowmetric analyses, genetic marking under certain experimental conditions - broad field for researches!
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Thanks for your attention!!!