The Antithrombotic and Fibrinolytic Activity of the Aqueous Extract From the Crude Latex of Ficus...

8/18/2019 The Antithrombotic and Fibrinolytic Activity of the Aqueous Extract From the Crude Latex of Ficus Pumila for Sending

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The Antithrombotic and

Fibrinolytic Activity of theAqueous Extract from theCrude Latex of Ficus pumila

(Moraceae)Vien Christian L. Lansangan, Kathrizza T. Mabutas,

Rachelle Z. Ong, Arnold M. Perlas,Joencio !. A"ostol

University of anto TomasFaculty of !harmacy


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"ntroduction

Clotting# $%"ortant &e%ostasis %echanis% 'hich"reents e(cessie blood loss

# Platelet Aggregation)*Te%"orar+ Platelet Plug# Actiation o Coagulation Cascade)*-tableibrin crosslin


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"ntroduction

Clotting

# $s har%ul 'hen the blood clot/thro%busdislodges and blocs blood essels

0rain 1 CV Accident/ -troe Lungs 1 Pul%onar+ e%bolis%


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"ntroduction

Current Treat%ents2. Anticoagulants

3. 4ibrinol+tic Agents

5. Anti Platelet Agents


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#otanical $escri%tion

Kingdo% Plantae

6iision Magnilio"h+ta

Class Magnilio"sida Order 7rticales

4a%il+ Moraceae

!enus Ficus

-"ecies pumila

87- 6A9RC, 3:22;


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ynonyms

Cree"ing 4ig

Cli%bing 4ig4ig $+

82;


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Mor%holo&ical $escri%tionCree"ing 4ig

# "rostrate or cli%bing shrub.

Leaes# T'o)raned

# Oate, 2.> to 5 c% long

# Round or heart sha"ed base# ?ntire or 'a+ %argins

# On erect branches, > to 2: c%

Late(# ascular li@uid 'ith rubber)lie @ualities

4ruits# Large and bro'n colored

# Partiall+ "ear)sha"ed to B

c% o length 82;


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'bectives

2. To deter%ine the antithro%botic andibrinol+tic actiit+ o Ficus pumila.

3. To deter%ine i the antithro%boticactiit+ o Ficus pumila is co%"arable to

that o he"arin.5. To deter%ine i theibrinol+tic actiit+ o

Ficus pumila is co%"arable to that o-tre"toinase.

. To deter%ine the "ossible %echanis% b+'hich Ficus pumila "reents thro%bosis.


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Methodolo&y


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Methodolo&y !re%aration of F!AE

8Perello et al., 3:::;


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Ficus pumila fruit


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F!AE

!7M-

4PA?la+er

$9-OL70L?PART$C7LAT?-

• Turbid light)bro'n solution


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$etermination of CaseinolyticActivity of F!AE

8Perello et al., 3:::;


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"n vitro clot dissolution

8!ong et al., 3:22;


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"n *itroClot$issolution


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Acute Toxicity tudy

To deter%ine doses o 4PA? to bead%inistered.

0ased o O?C6 T! 3> guidelines


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Tail Thrombosis


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Methodolo&y tatistical Treatmentof $ata

All statistical anal+ses 'ere "eror%ed at:.:> leel o signiicance.

The Anal+sis o Variance 8A9OVA; 'asused to deter%ine 'hether signiicantdierences across treat%ents e(isted.

Tue+Ds %ulti"le co%"arisons test 'asutilized to identi+ signiicant dierences

bet'een s"eciic treat%ents.


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,esults and $iscussion

Late( Eield5: ruits 'eighing 2:F5.2g +ielded

23.:%L o late(The "ercentage +ield is 2.22G8/';

Caseinol+tic Actiit+4PA? e(hibited an aerage caseinol+tic

actiit+ o :.2==7cas/%L ro% 3 trials inthe "resence o 23%M o c+steineHCaseinol+tic 7nit87cas; is the a%ount o enz+%e that "roduces an increase o one absorbance unit"er rninute in the assa+ conditions


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,esults and $iscussion0lood Clot 6issolution

Fi&ure -. Mean !ercenta&e $issolution across treatment levelsA direct dose)clot dissolution relationshi" or 4PA? 'as obsered. At 2::) and2>:%g/5%L, the G blood clot dissolution 'as signiicant. -tre"toinase 2:$7 'asstatisticall+ "roen to be %ore eectie then 4PA? in the dissolution o blood clots

ater 3h "eriod. 2>:%g 4PA? 'as e@uialent to >3G o 2:,:::$7 o -tre"toinase.


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,esults and $iscussionPreention o Carrageenan)$nduced Tail Thro%bosis

&e"arin &e"arin 4PA? 4PA? 4PA? 9-- 2::$7 2:$7 23I%g/g F:%g/g >:%g/g

Fi&ure /. ?ects o 4PA? and &e"arin on %ice tail Thro%bus Lengths83 hours ater carrageenan ad%inistration;


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Fi&ure 0. Mean Len&th of Thrombosis (over 0+day %eriod) A direct dose)"reention o tail thro%bosis relationshi" or 4PA? 'as

obsered.

%


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,esults and $iscussion

Preention o Carrageenan)$nduced TailThro%bosisTest Samples Doses Inhibition of thrombosis (%)

24h 48h 72h

Heparin 100IU 94 92 92

10IU 87 80 80

FPAE 127mg/kg 60 61 60

80mg/kg - - 45

50mg/kg - - -

Fi&ure 0. Table of Mean Len&th of Thrombosis (over 0+day %eriod)4PA? at 23I%g/g 'as able to signiicantl+ "reent carrageenan)induced

tail thro%bosis oer the 5 da+ "eriod. 4PA? at F:%g/g had signiicant actiit+onl+ on the third da+.

%


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Conclusion4PA? "ossesses antithro%botic actiit+ in

io as it signiicantl+ "reentedcarrageenan)induced %ice tail thro%bosis.

The "ossible %echanis% o action is direct

ibrin h+drol+sis b+ icin as "roen in theblood clot dissolution test.# 4icin 'as "resent in 4PA? and had a caseinol+ticactiit+ o :.2==7cas/%L

4PA? at 23I%g/g is as eectie as 2:$7and 2::$7 Co%%erciall+ Aailable &e"arin.The ibrinol+tic actiit+ o 2>:%g 4PA? is

e@uialent to >3G o -tre"toinase 2::$7


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,ecommendations

To deter%ine other "ossible %echanis%so action# "latelet aggregation

# aPTT and PT

7se "uriied icin instead o crude enz+%ee(tract.


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,eferences

0ee%eier et al. 82=F>; Carrageenin)induced thro%bosis inrats and %ice a %odelor testing antithro%botic substances Agents Actions 2BB1>2.

!ong et al. 83:22;. Anti)thro%bosis eect o diosgenin e(tract ro% 6ioscoreazingiberensis C.&. right in itro and in io, Ph+to%edicine, Volu%e 2F, $ssue B.

&agi%ori et al. 83::=;. $%"roing re@uenc+ o thro%bosis b+ altering blood lo' inthe carrageenan)induced rat tail thro%bosis %odel. Phar%acological research theoicial ournal o the $talian Phar%acological -ociet+, B:8;, 53:)535.

Perello, M., Arribere, M. C., Caini, 9. O., N Priolo, 9. -. 83:::;. Proteol+tic ?nz+%esro% the Late( o 4icus "u%ila L. 8Moraceae;. Latin A%erican Journal o Phar%ac+ , 2=8;, 3>I)3B3.

2;. Medicinal Plants o the Phili""ines. Manila 6e"t. oAgriculture N 9atural Resources.

PLA9T- Proile or 4icus "u%ila. 83:22;. Retrieed 4ebruar+ 2B, 3:22, ro% 7-6e"art%ent o Agriculture 9atural Resource Conseration -ericehtt"//"lants.usda.go

/aa/"roiles+%bol4$P73

http://plants.usda.gov/java/profile?symbol=FIPU2http://plants.usda.gov/java/profile?symbol=FIPU2http://plants.usda.gov/java/profile?symbol=FIPU2http://plants.usda.gov/java/profile?symbol=FIPU2http://plants.usda.gov/java/profile?symbol=FIPU2http://plants.usda.gov/java/profile?symbol=FIPU2http://plants.usda.gov/java/profile?symbol=FIPU2http://plants.usda.gov/java/profile?symbol=FIPU2


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