The 6420 Triple Quad LC/MS System

Routine, Robust and Reliable Quantitation

Agilent has the Newest Fleet of LC Triple Quads

A New Agilent LC Triple QuadLaunchedEvery YrFor last4 yrs

6460 Triple Quad

6430 Triple Quad

6420 Triple Quad

6490 Triple Quad

6460 Triple Quad

Per

form

ance

6420 Enhancements over Earlier 6410

• New Capillary front end:

faster polarity switching better ion

transmission

• New electronics, faster MRMs

• New Agilent rough pump• Upgradeable to a 6460 with

Agilent Jet Stream Technology

6420 Triple Quad LC/MS

The Agilent 6420 Triple Quad features fast and rugged electronics;Making it a workhorse for high throughput labs

Fast MRMs , dwell time of: 1 msec

Fast polarity switching : 30 msec

Fast Triggered MRM(for ten ion transitions): 50 msec

6420: Fast Electronics for High Sample Throughput

Future Proof Your LC Triple Quad Investment

Infinity LC

6420

Routine Analysis

Ultra-Trace Analysis

6430

Pe

rfo

rma

nc

e

Trace Analysis

Step 1

Step 2

6460

6460 Triple Quad

6430 Triple Quad

8X

2X

1X

S/N Spec

6420 Triple Quad

ProductUpgrade

OptionsInstalledin your

Lab

6430to 6460

Agilent Jet Stream

& High Mass Quads

Yes

6420to 6430

SecondTurbo

Yes

6420 Upgrade Possibilities

New 6420 LC Triple Quad Supports:

Agilent Upgrade Kits to 6430 and 6460 LC Triple Quads

Agilent Dynamic MRM

Agilent Fast Track Kits and Data Bases

Agilent Triggered MRM

The 6420 is preconfigured to accept a second turbo pump for enhanced sensitivity.

Higher vacuum in Q3 reduces noise and allows greater ion conductance in the collision cell.

Upgradeable by Design – 6400 Upgrades

6420 to 6430 UpgradeImproves Signal-to-Noise up to two-fold for some compounds

6430 to 6460 Add: Agilent Jet Stream Technology thermal confinement of ESI spray cone

25 0C

350 0C after 8 min

6430 to 6460 UpgradeSensitivity Spec: 4X higher

The 6460 mass range is 3,000 m/z:

Pegylated peptides

Acylated peptides

Lipopolysaccharides

Glycans

Natural Products

6430 to 6460 Upgrade: High Mass Quads – 3K

 Dynamic MRM creates powerful quantitative methods by grouping MRMs in retention time windows instead of time segments.

Dynamic MRM methods are created by importing compound specific MRMs and retention times from a Fast Track Data Base or worksheet

Dynamic MRM supports up to 4,000 MRMS for all 6400 Series Triple Quads

Dynamic MRM also supports polarity switching

Dynamic MRM groups MRMs in Retention Windows

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 161 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Retention Time Windows with overlapping MRMs

Dynamic MRM for the new 6420 Triple Quad

Agilent Fast Track Kit for Forensics

Includes a Dynamic MRM method, 200 compound data base with

optimal settings and RTs

FORENSIC/TOXICOLOGY TEST MIX – 24 compoundsCompound Name Precursor Ion Product Ion Collision Energy Ret TimeHydrocodone 300.2 199 29 1.4 methamphetamine 150.1 119 65 1.45 MDMA 194.1 163 9 1.468 Strychnine 335.2 184 41 1.629 MDEA 208.1 163 9 1.735 ………………

1 1

Counts vs. Acquisition Time (min)Acquisition Time (min)

1 1.2 1.4 1.6 1.8 2 2.2 2.4 2.6 2.8 3 3.2 3.4 3.6 3.8 4 4.2

AmphetamineMetham-phetamine

Trazodone

PCP

Norver-apamil

Verapamil

Methadone

Proadifen

Benzoyl-ecgonine

Cocaine

Forensics Test Mixture on the new 642025 pg of each on-column

152.1

77.0

105.0 219.0

m/z

60 100 140 180 220

209.1

165.1

triggered MRM Spectra

Triggered MRM (tMRM) – a Data Dependent Scan Data dependent execution of additional, confirmatory MRMs

2-Ethylanthraquinone

Cpd 1: peptide 3: +ESI MRM Frag=120.0V CID@20.0 (575.3000 -> 937.5000) HSA-tDMRM-1pep-02.d

Counts vs. Acquisition Time (min)1.9 2 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 3 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9 4 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 5

Triggered MRM in MassHunter Acquisition

Threshold

Additional MRMs used

for compound confirmation

Primary cycle (below threshold)

Triggered cycle (above threshold)

tMRM Product Ion Spectrum on any 6400 TripleA Re-Constructed Product Ion Spectrum that is Library Searchable

x103

876543210

80 100 120 140 160 180 200 220 240 260 280 300 320 340

334.2171.1

145.0

132.1

117.0

105.191.176.0

tMRM Product Ion Spectrum

Tebufenpyrad

x103

876543210

80

76.0

x103

876543210 80 100

91.1

x103

876543210 80 100 120

105.1

x103

876543210 80 100 120

117.0

x103

876543210

100 140

132.1

x103

876543210 80 100 120

119.0

x103

876543210 100 140 180

145.0 x103

876543210

100 140 180

147.0

x103

876543210 80 100 120 140 160 180

171.1

334 > 76 334 > 91 334 > 105 334 > 117 334 > 119

334 > 132 334 > 145 334 > 147 334 > 171

Benefits of triggered MRM

• More than ten times more sensitive than full scan MS/MS data

• Searchable with NIST MS/MS 2.0 Library

• Searchable with Agilent Accurate Mass MS/MS Library

• Compatible with custom, user created libraries

• MassHunter automatically creates custom triggered MRMlibrary from standards

1 1

Counts vs. Acquisition Time (min)Acquisition Time (min)

1 1.2 1.4 1.6 1.8 2 2.2 2.4 2.6 2.8 3 3.2 3.4 3.6 3.8 4 4.2

AmphetamineMetham-phetamine

Trazodone

PCP

Norver-apamil

Verapamil

Methadone

Proadifen

Benzoyl-ecgonine

Cocaine

Forensics Mixture with 6420 and tMRM Method

Triggered MRM actuated on red peak

@ 5ppb level: RSD=4.9% (N=3)

Triggered MRM for Compound Confirmation6420 tMRM: Benzoylecgonine, 25 pg on column

Mass-to-Charge (m/z)

100 150 200 250 300-1.2

-1

-0.8

-0.6

-0.4

-0.2

0

0.2

0.40.6

0.8

1

168.1

168.1

77.0

77.0105.0

105.0

290.1

Ref. Library Match Score = 95.1

Peak Product Ion Spectra

Library ReferenceProduct IonSpectra

DENVER (American Society for Mass Spectrometry conference), June 6, 2011

The new Agilent 6420 Triple Quad LC/MS System is a workhorse triple quad designed to be readily upgradable to the higher-performance 6430 and 6460 instruments.

The new 6420 is more productive than the previous 6410 model with faster electronics and new software features. Our popular dynamic MRM solution for grouping ion transitions by retention time has been enhanced with the addition of triggered MRM for simultaneous compound confirmation.

The Agilent 6420 is well suited for both routine and complex analyses such as toxicology and drugs of abuse testing. It is designed for round-the-clock operation to support labs running a large number of samples.

The 6420 replaces Agilent's popular Model 6410 triple quad. The 6420 maintains method and data compatibility with existing 6410 methods. The 6420 can be easily incorporated into high-productivity laboratories with multiple 6410 systems.

6420 Summary

THANK YOU!