Illegal substances and methods: performance-enhancing drugs and methods.
Testing of Performance Enhancing Drugs James Rosenberger September 22, 2014.
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Transcript of Testing of Performance Enhancing Drugs James Rosenberger September 22, 2014.
Commonalities
Wes Welker - AdderallManny Ramirez – human chorionic gonadotropin
Lance Armstrong – human growth hormone, steroidsAlex Rodriguez – PrimobolanRyan Braun – Testosterone
http://nfl.blog.chatsports.com/files/2014/05/welker.jpg
http://elflowlatino.com/wp-content/uploads/2014/05/rex.jpg
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Ryan Braun
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http://i.ytimg.com/vi/8UOcHEQ3z0U/0.jpghttp://i.usatoday.net/sports/_photos/2011/09/25/MLB-games-matter-after-playoff-spots-clinched-A6DTHLR-x-large.jpg
Frowned Upon
• Uneven playing field• “How [am I] supposed to compete when others were
taking PEDS?” –Jeff Cirillo, 2002
• Health Side Effects• Acne• Impaired liver function• Increase in estrogen• Male pattern baldness
• Sets poor example for children• Shortcuts• Young people (Under 21) should NOT take steroids.
Doping
• Steroids/HGH/Blood Doping/Stimulants• What do they do?• Make muscles grow faster• Harmful side affects to health• Reduce fatigue• Recover from injuries quicker• Most major sports have banned them
• But how does it really work?• How are we stopping them?
http://upload.wikimedia.org/wikipedia/en/8/8f/WADA_logo.jpg
Steroids in Baseball
• Thomas Boswell, 1988• “The most conspicuous example of a
player [Jose Canseco] who has made himself great with steroids.” –CBS Late Night
• Truth without portfolio• Couldn’t publish this in a newspaper
• Beat Writers heard rumors, but could never whistle blow• Bob Nightengale, July 1995
• “Steroids Become an Issue…”Jose Canseco
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Great Home Run Race of 1998
Barry Bonds Record 2001 Season
http://en.wikipedia.org/wiki/Barry_Bonds#mediaviewer/File:Barry_Bonds_756_Ball.png
http://espn.go.com/media/classic/2001/0910/photo/sosamcgwire_ct.jpg
Steroids
• Definition: Refers to a class of hormones with a classic poly-phenol ring structure.• Catabolic Steroids (glucocorticoids)• Produced in the adrenal cortex or made
synthetically. Various metabolic function use to treat inflammation.
• Anabolic Steroids• Synthetic steroid hormone that
resembles testosterone in promoting the growth of muscle.
Gonanehttp://commons.wikimedia.org/wiki/File:Gonane.svg
Anabolic Steroids
• “Contain a synthetically manufactured form of the hormone testosterone”• Primary male sex hormone• An enhanced rate of protein
synthesis leading to muscle accumulation• Reason why males carry more muscle
mass than women• 11mg per day vs ¼ mg per day.
Testosterone
http://upload.wikimedia.org/wikipedia/commons/c/ce/Testosteron.svg
Gonane
http://commons.wikimedia.org/wiki/File:Gonane.svg
(Simple) Biochemistry of Anabolic Steroids
• Testosterone binds to intracellular receptor site in cytosol• Form Receptor complex and it
migrates to cell’s nucleus• Attaches to cell’s DNA• Activates transcription of
specific genes• Increase synthesis of two
primary contractile proteins, actin and myosin
ANABOLICS, 10th edition, William Llewellyn
Human Growth Hormones (HGH)
• Naturally occurring protein hormone• Excessive amounts give user
benefits by stimulating• Protein synthesis for increased
muscle mass• Bone growth for stronger bones• Breakdown of fat cells
• Use of HGH has become increasingly popular due to the difficulty of detection
http://www.nutrasanus.com/images/GrowthHormoneLevels.gif
Human Growth Hormones (HGH)
• Important Side-Effects• Stress on liver and
kidney• Swelling in the limbs• Migraines and
Headaches• High blood pressure• Bloating• Arthritis-like symptons
• But what makes testing so difficult?
http://www.nutrasanus.com/images/GrowthHormoneLevels.gif
http://images.publicradio.org/content/2013/01/01/20130101_hgh2_33.jpg
Human Growth Hormone Testing
Isoform Testing• At present, only blood tests have
found positive results• Natural HGH weighs 20 kDaltons• Synthetic HGH weighs 22
kDaltons• NFL & MLB: positive cutoff is 1.8• Downside:
• Short detection window (18-24 hours)
• 10,000 tests have been done since 2004 with only 14 positives.
HGH Testing
• A new method with LC-MS/MS is being tested• Measures IGF-1, Insulin-like
Growth Factor – 1• Blood test• IGF-1 is the response from an
active pituitary gland and a byproduct of HGH.
http://www.steroidtimes.com/wp-content/uploads/2010/08/nrd2359-f2-300x270.jpg
Liquid Chromatography
• Separation!• Stationary phase – Packed in column• Mobile phase – Solvent (based on polarity)
http://www.sec.psu.ac.th/web-board/content/view_img.php?id=667
Mass Spectrometry
• LC Product fed in• Ionized• Electric Field is altered
• Light particles get curved too much
• Heavy particles ignore field
• Tolerances are hard to find as detectors measure m/z• Retention Time and m/z ratio
can narrow down search considerably
http://www.chemguide.co.uk/analysis/masspec/masspec.GIF
T/E Ratio
• Testosterone/Epitestosterone• Epitestosterone is a byproduct
of testosterone• Normal ratio is 1:1• Positive test for NFL/MLB is 4:1• Carbon Isotope test follow up.• Pharmaceutical testosterone has
slightly more Carbon 12.
http://www.ftmguide.org/images/Tmolecule.gif
Concerns for Future
• Testing is expensive• Can we lower the price?• Use chemical reactions on
manufactured kits?• Avoid high priced machines
• Drugs are developing every year and finding a test will always lag behind• Lower tolerances, but avoid
false positives
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Real Life Examples
• Last week: NFL added HGH Testing into Drug Testing Policy• Possibly lower tolerances
• Olympians in Sochi• A passport of every drug test
they have had.• Gives testing facility a
comparison
• Drug Screening for Jobs
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