www.lipotec.com blue biotechnology

DescriptionExtract obtained through biotechnology from the microorganism E. crustaceum isolated in Playa Paraíso, a rocky beach in Tenerife. eyedeline™ marine ingredient provides complete care of the eye contour area by reducing fluid extravasation and increasing the degradation of bilirubin. Moreover, it boosts collagen and elastin synthesis and decreases the formation of advanced glycation end products (AGEs), for a brighter and younger look.

AppearanceTransparent solution containing Extract.

INCIButylene Glycol, Water (Aqua), Plankton Extract.Preservative free.

Propertieseyedeline™ marine ingredient acts on three main evidences of tiredness and aging around the eyes by improving microcirculation and reinforcing the extracellular matrix. It rejuvenates the delicate skin and enhances the appearance of the eye contour area.

Applicationseyedeline™ marine ingredient can be incorporated into any product developed for global care of the eye contour area as it corrects the three main unaes-thetic signs: dark circles, puffiness and wrinkles.

ScienceThe look of the eyes transmits beauty and attractiveness and it is essential in interpersonal communication. As we age, the delicate skin around the eyes becomes even thinner and therefore more susceptible to age-related changes. Eye puffiness appears due to liquid retention as a consequence of higher permeability of blood vessels, which when added to bilirubin accumulation (from hemoglobin breakdown) induces vascular pigmentation and therefore dark circles. In addition, the deterioration of skin support proteins, like collagen and elastin, contributes to an increased visibility of such signs, besides the development of wrinkles.eyedeline™ marine ingredient is an active ingredient produced by the marine microorganism E. crustaceum isolated from the waters in Playa Paraíso (Tenerife). This biotechnological extract reduces fluid extravasation, increases the degradation of bilirubin, promotes collagen I and elastin production, and inhibits the formation of AGEs. As a result, it provides a complete care around the eyes acting on bags, dark circles and crow’s feet wrinkles, to achieve a younger and brighter look.

Dosage 1-4%

SolubilityWater soluble.

An impressive look in one touch

TenerifeSpain

eyedeline™

marine ingredient

Lessens eyebags and dark circles

Provides glow for a younger look

Smoothes eye contour lines

anti-dark circles

anti-eyebag

anti-wrinkles

0.11% Plankton

In vitro efficacy

In vivo efficacy

1. VASCULAR PERMEABILITY INHIBITIONVascular permeability was assessed on human umbilical vein endothelial cells grown on top of a porous membrane until a semipermeable vascular barrier was formed. Cells were treated with 1 µg/mL eyedeline™ marine ingredient concentrate and fluorescence levels were measured from the amount of (FITC)-Dextran permeated.

2. EFFECT ON THE EXTRACELLULAR MATRIX

• Collagen and elastin synthesisSynthesis of type I collagen was evaluated by an ELISA on fibroblasts treated with 1 or 5 µg/mL eyedeline™ marine ingredient concentrate. And elastin levels were measured by a dye-binding method with 0.1 or 1.0 µg/mL of the active ingredient concentrate.

• AGEs formationCollagen glycation was induced by incubating collagen with glucose and evaluated using an enzyme immunoassay that measured AGE adducts. This was done without (control) or with 1 or 100 µg/mL eyedeline™ marine ingredient concentrate.

1. REDUCTION OF DARK CIRCLES

2. DECREASE IN EYEBAGS

3. SMOOTHING WRINKLES

21 female volunteers (22-65 years old) showing dark circles applied a cream with 1% eyedeline™ marine ingredient on one side of the eye contour area, and a placebo cream on the other side, twice a day, for 28 days. High resolution photographs were taken under cross polarized light and the vascular component (red color) was analyzed.

A group of 20 female subjects (aged 41-66) showing eyebags used a cream with 4% eyedeline™ marine ingredient on half of the face around the eye and a placebo cream on the other half, twice a day, for 28 days. Fringe projection technique was used to evaluate the eyebags volume.

Skin topometry was determined by fringe projection in 18 female volunteers (40-54 years old) that applied a cream with 2% eyedeline™ marine ingredient or a placebo cream on the crow’s feet wrinkles twice a day, for 14 days. FOITS technique allowed the obtention of average roughness (Ra), mean wrinkle depth (Rz) and maximum wrinkle depth (Rt).

Decreased vascular permeability by 20.8%, linked to a reduction of edema

36% more effective than the placebo against dark circles

Significant effect on dermal proteins inductionCollagen I levels increased by 61.8% and elastin by 51.6%.

Preservation of matrix proteins functionalityeyedeline™ marine ingredient significantly diminishes the formation of AGE-collagen adducts.

Great visibleimprovement in eyebagsThe results were already evident after 14 days, reducing them by 8.2% after 28 days.

Decrease of all wrinkle parameterseyedeline™ marine ingredient provided a general smoothing effect reducing Ra by 9.0%, Rz by 8.7% and Rt by 9.8%.

0 days 28 days

Isaac Peral 17 Pol. Industrial Camí Ral - 08850 Gavà (Barcelona) Spain - Tel +34 93 638 80 00 - commercial@lipotec.com

Ed. 15A

© 2015 The Lubrizol Corporation.All Rights Reserved.

The trademarks referenced in this brochure are owned by The Lubrizol Corporation. While the information contained herein is believed to be reliable, there are no representations, guarantees, or warranties of any kind made as to its accuracy, suitability for particular applications, how the product(s) will perform in combination with other substance or in the User’s process or the results obtained. All expressed and implied warranties are disclaimed. Lubrizol and its affiliates MAKE NO WARRANTIES, EXPRESS OR IMPLIED, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. The User is solely responsible for ensuring that products marketed to consumers comply with all relevant laws and regulations and assumes all risk and liability of any use or handling of any materials. User agrees to indemnify and hold harmless Lubrizol and its affiliates for any and all actions arising from User’s use of any information including claims in this publication, including, but not limited to, use in advertising and finished product label claims, and not present this publication as evidence of finished product claim substantiation to any regulatory authority. It is the User’s sole responsibility to determine if there are any issues relating to patent infringement relating to the supplied information. Nothing contained herein is to be considered as permission, recommendation, nor as an inducement to practice any patented invention without permission of the patent owner.

eyedeline™

marine ingredient

120

100

80

60

40

20

0

AG

E le

vels

(%

)

eyedelineTM

marine ingredient concentrate(1 µg/mL)

eyedelineTM

marine ingredient concentrate(100 µg/mL)

Control

-19.4% -29.7%

0 days 14 days 28 days