tenderness - University of Kentucky

25

Transcript of tenderness - University of Kentucky

Meat

Ten

dern

ess

•#

1 Q

ua

lity

Co

nc

ern

•#

1 P

ala

tab

ilit

y

Co

nc

ern

fo

r C

on

su

me

rsC

on

su

me

rs

•C

os

ts t

he

Be

ef

Ind

us

try o

ve

r $

25

3

mil

lio

n a

nn

ua

lly

•G

ua

ran

tee

d T

en

de

r P

rod

uc

t

Measu

rin

g T

en

dern

ess

•O

bje

cti

ve

ly

–W

arn

er-

Bra

tzle

r S

hear

Fo

rce M

ach

ine

–½

” m

eat

co

re;

para

llel to

fib

er

para

llel to

fib

er

ori

en

tati

on

•S

ub

jec

tiv

ely

–S

en

so

ry P

an

el

–H

um

an

pers

pecti

ve

Wh

at

is t

en

dern

ess

•P

rote

ases e

nzym

es

•C

alc

ium

acti

vate

d

•C

alp

ain

s, calp

asta

tin

•D

eg

rad

e Z

-dis

k

•D

eg

rad

e Z

-dis

k

•M

yo

fib

ril fr

ag

men

tati

on

•O

ccu

rs p

re-

an

d p

ostm

ort

em

•5 –

6%

pro

tein

deg

rad

ati

on

/ d

in

h

um

an

s

Make t

hin

gs m

ore

ten

der

•P

eo

ple

will sp

en

d t

heir

liv

es a

nd

care

ers

searc

hin

g f

or

ways t

o im

pro

ve

ten

dern

ess a

nd

un

ders

tan

d t

he f

acto

rs

invo

lved

•W

ays t

o im

pro

ve t

en

dern

ess

–M

ak

e t

he

Sa

rco

me

res

lo

ng

er

–D

isru

pt

the

in

teg

rity

of

the

myo

fib

rils

–D

isru

pt

the

in

teg

rity

of

the

co

nn

ec

tiv

e

tis

su

e m

atr

ix

Wh

at

aff

ects

Ten

dern

ess

Die

tC

oo

ler

Aff

ects

Imp

lan

ts/ G

row

th

Pro

mo

tan

ts

Ag

ing

Mu

scle

F

un

cti

on

Co

ntr

acti

le

Sta

te

Co

okin

g

Meth

od

s

Ag

e o

f A

nim

al

Die

t

•V

itam

in D

3

•H

yp

oth

esis

; V

itam

in D

3w

ill ra

ise t

he

level o

f cir

cu

lati

ng

calc

ium

, th

us

acti

vati

ng

mo

re c

alc

ium

dep

en

den

t acti

vati

ng

mo

re c

alc

ium

dep

en

den

t p

rote

ases

•C

alp

ain

s =

acti

vate

d b

y c

alc

ium

•F

ed

th

e last

6 t

o 1

0 d

befo

re s

lau

gh

ter

Vit

am

in D

3

•In

cre

as

ed

pla

sm

a C

a c

on

ce

ntr

ati

on

s

(Sw

an

ek

et

al.

, 1

99

9;

Ka

rge

s e

t a

l., 1

99

9)

•In

cre

as

ed

te

nd

ern

es

s (

WB

SF

) b

y 0

.58

kg

an

d

se

ns

ory

pa

ne

l te

nd

ern

es

s b

y 0

.6 u

nit

s

(Sw

an

ek

et

al.

, 1

99

9;

Ka

rge

s e

t a

l., 1

99

9;

(Sw

an

ek

et

al.

, 1

99

9;

Ka

rge

s e

t a

l., 1

99

9;

Mo

ntg

om

ory

et

al.

, 2

00

0)

•N

o i

mp

rov

em

en

ts i

n t

en

de

rne

ss

(S

ca

ng

a e

t a

l., 1

99

9;

Re

ntf

row

et

al.

, 2

00

0;

We

rtz e

t a

l.,

20

01

)

•U

nd

er

4.5

-kg

WB

SF

co

nfi

de

nc

e l

ev

el

Gro

wth

Pro

mo

tan

ts/

Imp

lan

ts

•B

ee

f Im

pla

nts

•In

cre

as

e

Te

sto

ste

ron

e

•In

cre

as

e C

alp

as

tati

n

•Im

pla

nte

d s

tee

rs

•Im

pla

nte

d s

tee

rs

ha

d h

igh

er

WB

SF

v

alu

es

th

at

no

n-

imp

lan

ted

c

ou

nte

rpa

rts

(R

oe

be

r e

t a

l., 2

00

0;

Pla

tte

r e

t a

l., 2

00

3)

Gro

wth

Pro

mo

tan

ts/

Imp

lan

ts

•In

cre

ased

WB

SF

valu

es in

im

pla

nte

d

Bo

s in

dic

us c

att

le (

Barh

am

et

al., 2003)

•H

ow

ever,

un

der

4.5

-kg

•R

acto

pam

ine (

Payle

an

or

Op

tafl

exx)

•R

acto

pam

ine (

Payle

an

or

Op

tafl

exx)

do

es n

ot

aff

ect

ten

dern

ess;

sen

so

ry o

r W

BS

F (

McK

eit

h e

t al., 1988;

Sto

ller

et

al.,2

003;

Sch

roed

er,

2005)

Co

ole

r A

ffects

•T

alk

ed

ab

ou

t th

is i

n

the

co

nv

ers

ion

of

mu

sc

le t

o m

ea

t

•C

old

Sh

ort

en

ing

•C

old

Sh

ort

en

ing

•T

ha

w R

igo

r

•P

rob

lem

in

be

ef

an

d

lam

b

•E

lec

tric

al

Sti

mu

lati

on

Co

ntr

acti

le S

tate

•M

yo

sin

–A

cti

n

cro

ss

-bri

dg

ing

•R

igo

r m

ort

is

•A

ch

ille

s T

en

do

n•

Ac

hil

les

Te

nd

on

•P

elv

ic h

un

g

–T

en

ders

tretc

h

–L

essen

s t

he a

ffects

o

f ri

go

r

–N

ot

do

ne in

US

Mu

scle

Fu

ncti

on

•S

up

po

rt v

s.

Lo

co

mo

tio

n

•A

mo

un

t o

f c

on

ne

cti

ve

ti

ss

ue

•E

xte

nt

of

rig

or

•C

an

ge

t te

nd

ern

es

s

dif

fere

nc

es

wit

hin

a

giv

en

mu

sc

le o

r s

tea

k

•D

ou

ble

mu

sc

led

ca

ttle

•C

all

ipyg

e L

am

b

Po

st-

mo

rtem

Ag

ing

•D

ry v

s. W

et

Ag

ing

•D

ry =

meat

lab

& s

mall

m

eat

pro

cesso

rs

•W

et

= m

ajo

rity

of

meat

•W

et

= m

ajo

rity

of

meat

in t

he U

S

•D

ry =

co

nsid

era

ble

sh

rin

k

•W

et

≈ 2

0d

fro

m p

ackin

g

pla

nt

to g

rocery

sto

re

Ag

e o

f A

nim

al

•A

s a

ge

in

cre

as

es

; m

ea

t b

ec

om

es

le

ss

te

nd

er

(Mil

ler

an

d M

on

tgo

me

ry,

no

da

te)

Yo

un

ger

An

imals

no

da

te)

•In

so

lub

le c

oll

ag

en

•C

ros

s-l

ink

s a

re h

ea

t s

tab

le

•L

arg

er

fib

er

dia

me

ters

O

lder

An

imals

Co

llag

en

C

ross-l

inks

Very

Rare

/Ap

pro

x.

130

Very

Rare

/Ap

pro

x.

130°°FF

Rare

/Ap

pro

x.

140

Rare

/Ap

pro

x.

140°°FF

Med

ium

Rare

/Ap

pro

x.

145

Med

ium

Rare

/Ap

pro

x.

145°°FF

Well

Do

ne/A

pp

rox.

170

Well

Do

ne/A

pp

rox.

170°°FF

Med

ium

/Ap

pro

x.

160

Med

ium

/Ap

pro

x.

160°°FF

Very

Well

Do

ne/A

pp

rox.

180

Very

Well

Do

ne/A

pp

rox.

180°°FF

Deg

ree o

f D

on

en

ess

•C

om

po

sit

ion

of

meat:

70%

wate

r, 2

0%

p

rote

in, 8%

fat,

2%

ash

•H

igh

er

deg

ree o

f d

on

en

ess =

less w

ate

r an

d f

at

an

d f

at

•T

ou

gh

en

ing

of

co

ntr

acti

le p

rote

ins

•P

rob

lem

wit

h c

on

su

mers

•B

eef,

Po

rk, an

d C

hic

ken

Ph

ysic

al

Ch

an

ges d

uri

ng

C

oo

kin

g•

Up

on

co

ok

ing

m

yo

fib

rill

ar

pro

tein

s

tru

ctu

re c

ha

ng

es

•D

isin

teg

rati

on

of

fila

me

nts

as

fi

lam

en

ts a

s

tem

pe

ratu

re i

nc

rea

se

s–

Pro

tein

hard

en

ing

–A

pp

ea

rs o

ver

14

F

•C

on

ne

cti

ve

tis

su

e–

1/3

ori

gin

al le

ng

th

–C

oll

ag

en

sh

rin

kag

e

–M

ore

so

lub

le

Co

okin

g t

he T

en

der

cu

ts

•D

ry H

ea

t C

oo

ke

ry

•B

roil

•G

rill

•P

an

–F

ry•

Pa

n –

Fry

•S

tir

–F

ry

•R

oa

st

–B

e c

are

ful

of

deg

ree

of

heat

Hig

h H

ea

tL

ow

He

at

Co

okin

g t

he T

ou

gh

Cu

ts

•M

ois

t H

ea

t C

oo

ke

ry

•L

ow

he

at,

lo

ng

tim

e

–P

reven

t p

rote

in

hard

en

ing

•B

rais

e

–S

team

typ

e h

eat

•S

tew

ing

–C

over

wit

h liq

uid

Ren

tfro

w’s

Reco

mm

en

dati

on

s

•J

us

t to

th

e p

oin

t w

he

re a

go

od

ve

t c

an

’t s

av

e i

t!!

ca

n’t

sa

ve

it!

!

•B

ee

f =

Me

diu

m R

are

•P

ork

= M

ed

ium

•C

hic

ke

n =

Fri

ed

or

BB

Q

Oth

er

thin

gs t

o t

hin

k a

bo

ut!

•G

en

eti

cs

= 4

5%

he

rita

ble

–G

en

eS

TA

R, Ig

en

ity, E

PD

’s?

•M

ec

ha

nic

al

Te

nd

eri

ze

rs

–C

ub

ers

or

Jaccard

–C

ub

ers

or

Jaccard

•C

he

mic

al

Te

nd

eri

ze

rs

–P

ap

ain

(p

ap

ya),

Bro

meli

n (

pin

eap

ple

), F

icin

(fi

g)

•M

ari

na

tin

g f

or

Te

nd

ern

es

s

–S

oft

en

s c

on

necti

ve t

issu

e, &

in

cre

ases H

2O

in

take

See y

a a

t la

bS

ee y

a a

t la

b