target Exon 1 ATG
6
Plasmid ID Gene targeted Talen Sequence (Left talen/spacer/right talen) binding sequences tag Method Segment I. Segment II. Segment III. Segment IV. SD_14 SORL1_ATG-Left Set1 T GCAGTAGCGTTCGCC cgaacatggcgacac GGAGCAGCAGGAGGG A GCAG TAGC GTTC GCC GFP Musunuru- Cowan SD_15 SORL1_ATG-right Set1 T GCAGTAGCGTTCGCC cgaacatggcgacac GGAGCAGCAGGAGGG A CCCT CCTG CTGC TCC RFP Musunuru- Cowan target Exon 1 ATG
Transcript of target Exon 1 ATG
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Plasmid ID Gene targeted Talen Sequence (Left talen/spacer/right talen)
binding sequences
tag MethodSegment I. Segment II. Segment III. Segment IV.
SD_14 SORL1_ATG-Left Set1 T GCAGTAGCGTTCGCC cgaacatggcgacac GGAGCAGCAGGAGGG A GCAG TAGC GTTC GCC GFP Musunuru-
Cowan
SD_15 SORL1_ATG-right Set1 T GCAGTAGCGTTCGCC cgaacatggcgacac GGAGCAGCAGGAGGG A CCCT CCTG CTGC TCC RFP Musunuru-
Cowan
target Exon 1 ATG
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parental GFP control
TALEN SORL1
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16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 17 18 19
WT homKO
het KO
het KO
Primer for screening
TALsorlafw: AGAAAGTGCGCGAAAGGGAC 230 bp vor ATG
TALsorlarev: TTGCAAAACTGCTCACCTGTC 301 bp nach ATG
Produkt: 531 bp
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clone 17 – ATG KO
clone 6 – ATG WT
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250
50
SORLA
α-tubulin