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Targe elector EGFR Mutation Test Kit A highly sensitive test kit for … · 2020-04-19 · Targe...
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Target Selector ™ EGFR Mutaon Test Kit for rare mutaons in blood and ssue www.biocept.com EGFR Tesng This Research Use Only tesng kit detects EGFR mutaons in DNA derived from blood plasma or FFPE ssue secons to give insight into cancer characteriscs and provide Biomarker status of tumors such as non-small cell lung cancer. Benefits of Biocept Target Selector ™ Switch-Blocker Technology • Enrichment of cancer biomarker mutaons (Del19, L858R, T790M) in a high wild-type background • Suppression of wild-type DNA amplificaon • Proofreading DNA Polymerase resulng in lower false posives • Avoids damaged DNA templates (such as deaminated cytosines) • Ultra-high sensivity and specificity • Combinaon with Sanger sequencing for orthogonal verificaon of the enriched mutaon • Detecon of Del19, L858R, T790M as well as Wild-Type are quantave Performance with ctDNA • Stability: 1 year • Sensivity: 0.05% mutant allele fracon -single copy detecon of mutant in up to 14000 copies of wild-type DNA background • Specificity: >99% • Effecve range: 10pg-50ng DNA FFPE DNA preparaon and performance • Eliminates need for macrodissecon of FFPE ssue by enriching mutaons and blocking wild-type DNA amplificaon • Sensivity thresholds have been established to account for arfacts introduced by formaldehyde fixaon. Mutant allele frequencies below these cut-offs are considered negave for the mutaon. » Del19 cutoff: 0.17% » L858R cutoff: 0.17% » T790M cutoff: 2.87% These thresholds are provided as a guideline but every research facility/laboratory should establish their own thresholds based on their own criteria. • Specificity: >98.4% • Effecve range: 10pg-50ng DNA A highly sensive test kit for accurate results
Transcript of Targe elector EGFR Mutation Test Kit A highly sensitive test kit for … · 2020-04-19 · Targe...
Target Selector™ EGFR Mutation Test Kitfor rare mutations in blood and tissue
www.biocept.com
EGFR TestingThis Research Use Only testing kit detects EGFR mutations in DNA derived from blood plasma or FFPE tissue sections to give insight into cancer characteristics and provide Biomarker status of tumors such as non-small cell lung cancer.
Benefits of Biocept Target Selector™ Switch-Blocker Technology• Enrichment of cancer biomarker mutations (Del19,
L858R, T790M) in a high wild-type background
• Suppression of wild-type DNA amplification
• Proofreading DNA Polymerase resulting in lower false positives
• Avoids damaged DNA templates (such as deaminated cytosines)
• Ultra-high sensitivity and specificity
• Combination with Sanger sequencing for orthogonal verification of the enriched mutation
• Detection of Del19, L858R, T790M as well as Wild-Type are quantitative
Performance with ctDNA• Stability: 1 year
• Sensitivity: 0.05% mutant allele fraction -single copy detection of mutant in up to 14000 copies of wild-type DNA background
• Specificity: >99%
• Effective range: 10pg-50ng DNA
FFPE DNA preparation and performance• Eliminates need for macrodissection of FFPE tissue
by enriching mutations and blocking wild-type DNA amplification
• Sensitivity thresholds have been established to account for artifacts introduced by formaldehyde fixation.
Mutant allele frequencies below these cut-offs are considered negative for the mutation.
» Del19 cutoff: 0.17% » L858R cutoff: 0.17% » T790M cutoff: 2.87%
These thresholds are provided as a guideline but every research facility/laboratory should establish their own thresholds based on their own criteria.
• Specificity: >98.4%
• Effective range: 10pg-50ng DNA
A highly sensitive test kit for accurate results
Target Selector™ EGFR Mutation Test Kitfor rare mutations in blood and tissue
*For Research Use Only. Not for use in diagnostic procedures.© 2020 Biocept, Inc. All rights reserved. Target Selector and CEE-Sure are trademarks of Biocept, Inc.MKTG-0088.01
Customer Service 888-332-7729 • FAX 877-300-1761Biocept, Inc.5810 Nancy Ridge Drive, San Diego, CA 92121
www.biocept.com
Target Selector™ EGFR Mutation Test Kit—Part Number: PN2193 This Research Use Only testing contains reagents to test 24 research samples for each target selector assay. It includes 12 reactions for standards, 1 NTC and 24 test samples for a total of 37 reactions.
Workflow
Reagent Part number Volume total (µl) Storage (°C)
Del19 Oligo Mix 2271 135 -20
L858R Oligo Mix 2272 135 -20
T790M Oligo Mix 2273 135 -20
Wild Type Oligo Mix 2274 135 -20
Premix 2278 275 -20
Premix with MgCl2 2280 275 -20
Selector DNA Polymerase 2290 75 -20
Del19 Sequencing Primer 2401 30 -20
L858R Sequencing Primer 2402 30 -20
T790M Sequencing Primer 2403 30 -20
Del19 Standard A 2391 150 -20
L858R Standard A 2392 150 -20
T790M Standard A 2393 150 -20
Wild Type Standard A 2394 150 -20
Standard A Dilution Buffer 2300 1200 -20
DNA Suspension Buffer 2390 1200 -20
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T-Red A-Green C-Blue G-Black
DNA Isolation
or
Patented Switch-Blocker Technology
qPCR Mutation Detection
Sanger Sequencing
Target Selector™ EGFR Mutation Test Kit
To learn more and order, please visit https://biocept.com/kits/egfr/
