SUPPORTING INFORMATION Mediator-free CV experiments were performed on Pt/GOx-PVA in nitrogen...

SUPPORTING INFORMATION Mediator-free CV experiments were performed on Pt/GOx-PVA in nitrogen saturated
SUPPORTING INFORMATION Mediator-free CV experiments were performed on Pt/GOx-PVA in nitrogen saturated
SUPPORTING INFORMATION Mediator-free CV experiments were performed on Pt/GOx-PVA in nitrogen saturated
SUPPORTING INFORMATION Mediator-free CV experiments were performed on Pt/GOx-PVA in nitrogen saturated
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Transcript of SUPPORTING INFORMATION Mediator-free CV experiments were performed on Pt/GOx-PVA in nitrogen...

  • Supplementary Material (ESI) for Analyst This journal is (C) The Royal Society of Chemistry 2010

    SUPPORTING INFORMATION

    Mediator-free amperometric glucose biosensor based on glucose oxidase

    entrapped in poly(vinyl alcol) matrix

    Maria Rachele Guascito*, Daniela Chirizzi, Cosimino Malitesta, Elisabetta Mazzotta Dipartimento di Scienza dei Materiali, Università del Salento, via Arnesano 73100 Lecce (Italy) Tel 0039 0832 297075; Fax 0039 0832 297100; maria.rachele.guascito@unisalento.it

    Fig. S1 – CV curves between -630 and -200 mV on Pt/GOx-PVA (red curve) and on Pt/FAD-

    PVA (black curve) electrodes in nitrogen saturated PBS (pH 7.0, I = 0.2) at scan rate of 50

    mV/s.

  • Supplementary Material (ESI) for Analyst This journal is (C) The Royal Society of Chemistry 2010

    Fig. S2 – Plot of I ( ) and II (•) current peak pairs against scan rate v (range 5 – 500 mV/s)

    obtained in CV experiments on Pt/GOx-PVA in nitrogen saturated PBS (pH 7.0, I = 0.2).

    Fig. S3 – Plot of ∆Epa(I), ∆Epa(II), ∆Epc(I) and ∆Epc(II) against logarithm of scan rate (log v)

    (range 10 – 400 mV/s for ∆Ep(I) and 25 – 500 mV for ∆Ep(II)). ∆E values represent the

    difference between each Epa and Epc values and the correspondent Esurf. Experimental

    conditions as in Figure S2.

  • Supplementary Material (ESI) for Analyst This journal is (C) The Royal Society of Chemistry 2010

    Fig. S4 – Shift of Epa(I)/Epc(I) pair (■) and Epa(II)/Epc(II) pair (•) with pH in the range 3-8.

    Experimental conditions as in Figure S2.

    Fig. S5 – Plot of anodic current peak ipa against the square root of scan rate v in the interval

    10 – 200 mV/s (a) and of anodic potential peak Epa against logarithm of scan rate (log v)

    (range 25 – 500 mV/s). CV experiments were performed on Pt/GOx-PVA in nitrogen

    saturated PBS (pH 7.0, I = 0.2) in glucose 10 mM.

  • Supplementary Material (ESI) for Analyst This journal is (C) The Royal Society of Chemistry 2010

    Fig. S6 – CV curves in not deaerated PBS (pH 7.0, I = 0.2) without (dotted curve) and with

    (solid curve) glucose 10 mM on Pt/GOx-PVA electrode.

    Fig. S7 – Amperometric responses of Pt/GOx-PVA electrode to glucose (Glu) 2 mM and to

    fructose (Fru), sorbitol (Sor), uric acid (UA) and ascorbic acid (AA) 1 mM in stirred and

    nitrogen saturated PBS (pH 7.0, I = 0.2). Measurements were performed in PAD mode (600

    mV for 0.2 s; -630 mV for 0.2 s; detection potential: -400 mV).

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