Supplementary Figures. Orikasa and Oda
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Supplementary Figures. Orikasa and Oda Elution profiles of three chromatographies of ion-exhange (CM- Toyopearl), hydrophobic interaction (Butyl-Toyopearl) and gel filtration (Toyopearl HW-55). Fraction number Enzyme activity (U/ml) Enzyme activity (U/ml) Enzyme activity (U/ml) CM-Toyopearl Butyl- Toyopearl Toyopearl HW- 55 Enzyme activity (U/ml) - - - NaCl (mM) - - - (NH 4 ) 2 SO 4 (% saturation)
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CM-Toyopearl. Enzyme activity (U/ml). - - - NaCl (mM). Butyl-Toyopearl. Enzyme activity (U/ml). - - - (NH 4 ) 2 SO 4 (% saturation). Toyopearl HW-55. Enzyme activity (U/ml). Enzyme activity (U/ml). Fraction number. Supplementary Figures. Orikasa and Oda - PowerPoint PPT Presentation
Transcript of Supplementary Figures. Orikasa and Oda
Supplementary Figures.
Orikasa and Oda
Elution profiles of three chromatographies of ion-exhange (CM-Toyopearl), hydrophobic interaction (Butyl-Toyopearl) and gel filtration (Toyopearl HW-55).
Fraction number
En
zym
e a
ctiv
ity (
U/m
l)E
nzy
me
act
ivity
(U
/ml)
En
zym
e a
ctiv
ity (
U/m
l)
CM-Toyopearl
Butyl-Toyopearl
Toyopearl HW-55
En
zym
e a
ctiv
ity (
U/m
l)-
- -
Na
Cl (
mM
)-
- -
(NH
4)2S
O4
(% s
atu
ratio
n)
