Supplementary Figure 5 Hori et al.

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Supplementary Figure Hori et al. cted Brain Storage and Sectioning Protocol (Illustr Dissected out Brain Regions Liquid Nitroge n Brain immersed in O.C.T. and 0.1 M PB (containing 20% sucrose) solution 2-Methylbutane (Wako Pure Chemical Industries Ltd., Osaka) To make PB: (68.99 g of NaH 2 PO 4 .H 2 0, Merck, was dissolved in final volume of 5 L Gelatin coated slides made in-house (MATUNAMI MICROSLIDE GLASS SUPERFROST S2442) Sectionin g OCT block stored at -80ºC Sectioning performed at -30ºC Mountin g

description

Dissected Brain Storage and Sectioning Protocol (Illustrated). Dissected out Brain Regions. 2-M ethylbutane (Wako Pure Chemical Industries Ltd., Osaka). Brain immersed in O.C.T. and 0.1 M PB (containing 20% sucrose) solution. - PowerPoint PPT Presentation

Transcript of Supplementary Figure 5 Hori et al.

Page 1: Supplementary  Figure 5 Hori et al.

Supplementary Figure 5Hori et al.

Dissected Brain Storage and Sectioning Protocol (Illustrated)

Dissected out Brain Regions

Liquid Nitrogen 

Brain immersed in O.C.T. and 0.1 M PB (containing 20% sucrose) solution

2-Methylbutane (Wako Pure Chemical

Industries Ltd., Osaka) 

To make PB: (68.99 g of NaH2PO4.H20, Merck, was dissolved in final volume of 5 L

Gelatin coated slides made in-house(MATUNAMI MICROSLIDE GLASS

SUPERFROST S2442)

Sectioning

OCT block stored at -80ºC

Sectioning performed at -30ºC

Mounting