Supplemental materials and methods...Aug 31, 2019 · Supplemental materials and methods Sample...
Transcript of Supplemental materials and methods...Aug 31, 2019 · Supplemental materials and methods Sample...
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Supplemental materials and methods
Sample collection, preservation, and RNA extraction
Specimens were preserved during field collections in RNAlater (Ambion) according to the
manufacturer’s recommendations, flash frozen at -80 C, or directly placed into 0.5 mL TRIzol for RNA
extraction, and total RNA was extracted with established protocols, homogenizing LN2-frozen
samples directly within 0.5-1.0 mL TRIzol (Riesgo et al., 2012b, 2012a). Tissues with RNA yields
expected to be within the sensitivity range of fragment analysis were routinely QC’d by Agilent
Bioanalyzer 2100, using RNA 6000 Pico or Nano kits (Agilent, Inc, cat. #s 5067-1511 and 5067-1513).
Polyadenylated mRNA was enriched using the Dynabeads™ mRNA Purification Kit (ThermoFisher,
Inc., cat. # 61006), eluting in up to 10 uL of 10 mM Tris-HCl, pH 7.5.
Transcriptome library generation
Except where specified, mRNA was converted into fragmented cDNA libraries and indexed
for Illumina sequencing using an automated library construction robot, the Apollo 324 (originally
manufactured by IntegenX Inc., then Wafergen, Inc., and currently by TaKaRa Bio, Inc). We used the
PrepX RNA-seq kit (TaKaRa, cat. # 640096), which RNAse III-fragments mRNA and performs single-
stranded adapter ligation prior to reverse transcription, to prepare up to 8 libraries at once, and
used manufacturer-provided indexing primers to assign sample barcodes. Libraries were QC’d using
the KAPA Library Quantification kit (KAPA Biosciences, Inc. KK4824, now Roche cat. # 07960140001),
and the Agilent 2100 High Sensitivity DNA kit (Agilent, Inc. cat. # 5067-4626). Pools were prepared
with plexity 6-12, and sequenced as paired-end 150 bp or 250 bp reads on Illumina HiSeq 2500
instruments, typically as rapid runs.
Whole genome and transcriptome amplified samples
For the gnathostomulid Tenuignathia rikerae, we attempted a genome assembly from DNA
amplified from a single individual by multiple displacement amplification. In brief, a single RNAlater
preserved specimen was rinsed in distilled water, and kept in a lens of ~2 μL, to which alkaline lysis
buffer from the Qiagen REPLI-g Single-Cell Kit (Qiagen, Inc., cat. # 150343) was directly added, and
DNA amplified as per manufacturer’s recommendations with the single-cell protocol, incubating at
30 C for 8 hours. MDA product was QC’d and confirmed to be HMW (70 kbp+) on an Agilent
Genomic DNA TapeStation 2200 kit (Agilent, Inc. cat. # 5067-5365). One aliquot was taken and
fragmented to ~350 bp using a Covaris S220 focused ultrasonicator and prepared as an Illumina
short-insert genomic DNA library using a previously described protocol for end-repair/A-tailing and
ligation of Y-shaped adapters (Neiman et al., 2012), followed by limited library amplification with
indexing primers and KAPA HiFi polymerase (KK2103). 1 μg of MDA product was processed into a
mate-pair library using the Illumina Nextera Mate-Pair Library Preparation Kit (Illumina, Inc. cat. #
FC-132-1001), following the manufacturer’s gel-free protocol, and amplifying the final Illumina
library with indexing oligos and the KAPA HiFi polymerase. Adapter sequences were trimmed from
the mate-pair library using NextClip (Leggett et al., 2014) using default parameters, and from the
short-insert library using Trimmomatic as described above. An initial assembly using SPAdes v3.2.0
was created running in single-cell mode; however, the assembly failed to finish at the k-mer size step
of 55. We therefore took the error-corrected reads output by SPAdes, and used these as inputs for
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assembly with IDBA_UD v1.1.1 (Peng et al., 2012). The resulting assembly remained highly
fragmented (1,691,903 scaffolds of which 1,140,751 were
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groups which contained more than 50 sequences and 50 columns. At this stage, we reconstituted
the orthogroups using the original, untrimmed sequences, and used the L-INS-I algorithm of the
MAFFT aligner (Katoh and Standley, 2013) to produce new multiple sequence alignment. These we
again masked with ZORRO, this time retaining all columns with a confidence score of > 0.5, and from
these masked alignments produced gene trees with RAxML, called with the PROTCATLGF model and
producing support values with 50 rapid bootstrap replicates. These gene trees and their originating
alignments were further curated with python scripts from the Phylogenomic Dataset Construction
(PDC) pipeline (Yang and Smith, 2014), calling the following three scripts in succession within the
working directory as such:
‘python trim_tips.py . .tre 5 10’ (removing all tree tips that were >5x longer than their sister, as well
as all tips that were over branch length 10)
‘python mask_tips_by_taxonID_transcripts.py . . y’ (masking all sequences from the same species
that formed a monophyletic or paraphyletic group, retaining the one with the longest proportion of
aligned residues)
‘python cut_long_internal_branches.py . tt.mm 2 30 cut_2’. (cutting all gene families into smaller
groups of size at least 30 if an edge within the gene tree had branch length of 2 or longer)
From this process survived 5,578 gene trees. These were then parsed to extract
unambiguously orthologous groups, using the criterion implemented in the Unrooted Phylogenetic
Orthology (UPhO) algorithm (Ballesteros and Hormiga, 2016), yielding 5,511 final orthogroups
comprising 195 taxa.
Matrix construction
To construct the pan-metazoa matrix, we selected a set of 422 alignments from the initial
set of 1,034 that were seen to have at least 100 sequences, by calling the MARE algorithm, initially
concatenating the 1,034 with phyutility’s ‘-concat’ option (Smith and Dunn, 2008), converting the
resulting NEXUS file into a FASTA in BioPython and extracting the partition list with vi regular
expressions, and running MARE v0.12 with the options ‘-t 100’ ‘-d 0.5’ to force retention of all taxa
and select for a matrix judged to be of adequate (initially at least 100,000 residues) size. Inspection
of this matrix and initial trees, and a contemporaneous publication reporting increased
compositional artifacts from including outgroups more distant than Choanoflagellata (Pisani et al.,
2015), led us to remove 28 taxa judged to be redundant representatives of their lineage and/or too
sparsely represented in the matrix, plus all non-choanoflagellate outgroups. The initial 422-gene
matrix of 106,186 residues was split into 10 nearly equally sized parts using BioPython, as a rough
parallelization of this job which ran very slowly as a serial process on the intact matrix, and BMGE
v1.12 was run with options ‘-g 0’, ‘-h 0.5’, ‘-m BLOSUM30’, and ‘-s FAST’ to retain all gapped sites,
but trim those that failed its approximate non-stationarity test, and those judged to be entropic
relative to the BLOSUM30 matrix (Criscuolo and Gribaldo, 2010). These parts were then re-joined
using BioPython to retain the final matrix. As reported in the main text, this process was performed
both before and after the deletion of the 28 taxa, with different results on the proportion of sites
retained.
For our subclade-specific matrices, we considered from among the set of 5,511 UPhO groups
a smaller set of 3,824 groups with at least 50 sequences, and created initial sets using the arbitrary
taxon inclusion criteria specified within the main text. MARE was used as described above to further
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reduce these sets to matrix sizes deemed appropriate for CAT+GTR analysis (approximately 50-100k
sites), tuning the ‘-d’ parameter to effect this result, and the resultant matrix was then treated with
BMGE prior to phylogenetic analysis, again as called above.
Phylogenetic analyses
In general, CAT+GTR+Г4 analyses were conducted using the PhyloBayes-MPI v1.6j release (Lartillot et al., 2013), starting each chain without specifying an initial tree, specifying the model with ‘-cat’ ‘-gtr’, removing constant sites with the ‘-dc’ option, and running each generally with 10 to 20 MPI processes. Dayhoff-6 recoded analyses were conducted with the same default parameters, but in Phylobayes v4.1c serial (Lartillot et al., 2009), specifying ‘-dayhoff6’ to initiate recoding. To trim suspected rogue taxa, we first used the RogueNaRok algorithm (Aberer et al., 2013) with default parameters to inspect .treelist files, and removed the species thus identified using the ‘-prune’ option of phyutility, prior to a new summary. IQ-tree (Nguyen et al., 2015) was run using specified versions, typically as a multithreaded
process using ‘-nt 10’ or AUTO, and selecting ‘-bb 1000’ and ‘-bnni’ to run 1,000 UFboot2 replicates
corrected with nearest-neighbor interchanges. Models were specified with the ‘-m’ parameter as
described in figure captions; in general, whenever the PMSF model was applied (Wang et al., 2018),
it was used to approximate the C60+LG+FO+R4 profile mixture model, and was called using an initial
guide tree inferred with ‘-m LG4X+FO+R4’.
References
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Supplementary Figure and Table Captions
Figure S1 – Maximum Likelihood cladogram from concatenated analysis of all 3,824
trimmed orthologue alignments with greater than 50 species (201 taxa, 1,263,500
sites), inferred in IQ-tree v 1.6.3 under the LG4X+R4+FO model, with 1,000 UFboot2
replicates corrected with NNI.
Figure S2 – Maximum Likelihood cladogram from the pan-Metazoa matrix with no
taxon or site trimming (195 taxa, 106,186 sites), inferred in IQ-tree v1.6.2 under the
PMSF approximation of the C60+LG+FO+R4 model, with 1,000 UFboot2 replicates
corrected with NNI. In this and all other PMSF-profile derived trees, an initial guide
tree was inferred under the LG4X mixture model.
Figure S3 – Cladogram from posterior consensus summary of CAT+GTR+Γ4 analysis
of post-BMGE taxon-pruned, 43,011 site pan-Metazoa matrix, with rogue taxa masked
prior to summary. Two chains were used to form this consensus, sampling every 10
generations, discarding the first 2,000 as burn-in, yielding a maxdiff of 0.309244.
Figure S4 – Cladogram from posterior consensus summary of CAT+GTR+Γ4 analysis
of post-BMGE taxon-pruned, 43,011 site pan-Metazoa matrix, with no rogue taxon
masking prior to summary. Two chains were used to form this consensus, sampling
every 10 generations, discarding the first 2,000 as burn-in, yielding a maxdiff of
0.309244.
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Figure S5 – Cladogram from Maximum Likelihood analysis of the post-BMGE taxon-
pruned, 43,011 site pan-Metazoa matrix, inferred in IQ-tree v1.6.2 under the PMSF
approximation of the C60+LG+FO+R4 model, with 1000 UFboot2 replicates corrected
with NNI.
Figure S6 – Cladogram from posterior consensus summary of CAT+GTR+Γ4 analysis
of pan-Metazoa matrix M, but with no rogue taxon masking prior to summary. Three
chains were used to form this consensus, sampling every 10 generations and
discarding the first 14,000 as burn-in, yielding a maxdiff of 0.21288.
Figure S7 - Cladogram from posterior consensus summary of CAT+GTR+Γ4 analysis
of pan-Metazoa matrix M, with rogue taxon masking prior to summary, and full pp and
taxon labels. Three chains were used to form this consensus, sampling every 10
generations and discarding the first 14,000 as burn-in, yielding a maxdiff of 0.21288.
Figure S8 – Cladogram from posterior consensus summary of CAT+GTR+Γ4 analysis
of pan-Metazoa matrix M, recoded into in Dayhoff-6 groups, the same as presented in
Figure 2b, but with no rogue taxon masking prior to summary. Three chains were
used to form this consensus, sampling every 10 generations and discarding the first
2,100 as burn-in, yielding a maxdiff of 0.543937.
Figure S9 – Cladogram from posterior consensus summary of CAT+GTR+Γ4 analysis
of pan-Metazoa matrix M, recoded into in Dayhoff-6 groups, the same as presented in
Figure 2b, with rogue taxon masking prior to summary. Three chains were used to
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form this consensus, sampling every 10 generations and discarding the first 2,100 as
burn-in, yielding a maxdiff of 0.228175.
Figure S10 -- Maximum Likelihood cladogram derived from pan-Metazoa matrix M,
inferred in IQ-tree v1.6.2 under the PMSF approximation of the C60+LG+FO+R4 model,
with 1000 UFboot2 replicates corrected with NNI.
Figure S11 -- Cladogram from posterior consensus summary of CAT+GTR+Γ4
analysis of “Spiralia” matrix S, the same as presented in Figure 3, but with no rogue
taxon masking prior to summary. Two chains were used to form this consensus,
sampling every 10 generations and discarding the first 6,000 as burn-in, yielding a
maxdiff of 0.146158.
Figure S12 -- Cladogram from posterior consensus summary of CAT+GTR+Γ4
analysis of “Spiralia” matrix S, the same as presented in Figure 3, with rogue taxon
masking prior to summary, and full pp and taxon labels. Two chains were used to
form this consensus, sampling every 10 generations and discarding the first 6,000 as
burn-in, yielding a maxdiff of 0.140087.
Figure S13 -- Cladogram from posterior consensus summary of CAT+GTR+Γ4
analysis of “Spiralia” matrix S, recoded into Dayhoff-6 groups, with rogue taxon
masking prior to summary. Two chains were used to form this consensus, sampling
every 10 generations and discarding the first 2,000 as burn-in, yielding a maxdiff of
0.0884638.
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Figure S14 -- Maximum Likelihood cladogram derived from “Spiralia” matrix S,
inferred in IQ-tree v1.6.2 under the PMSF approximation of the C60+LG+FO+R4 model,
with 1000 UFboot2 replicates corrected with NNI. The position of the root is arbitrarily
drawn between the clade of Syndermata+Micrognathozoa+Chaetognatha and the
remaining taxa.
Figure S15 -- Cladogram from posterior consensus summary of CAT+GTR+Γ4
analysis of “Ecdysozoa” matrix E, the same as presented in Figure 4, but with no
rogue taxon masking prior to summary. Two chains were used to form this
consensus, sampling every 10 generations and discarding the first 2,000 as burn-in,
yielding a maxdiff of 0.552004.
Figure S16 -- Cladogram from posterior consensus summary of CAT+GTR+Γ4
analysis of “Ecdysozoa” matrix E, the same as presented in Figure 4, with rogue
taxon masking prior to summary, and full pp and taxon labels. Two chains were used
to form this consensus, sampling every 10 generations and discarding the first 2,000
as burn-in, yielding a maxdiff of 0.0839663.
Figure S17 -- Cladogram from posterior consensus summary of CAT+GTR+Γ4
analysis of “Ecdysozoa” matrix E, recoded into Dayhoff-6 groups, with rogue taxon
masking prior to summary. Two chains were used to form this consensus, sampling
every 10 generations and discarding the first 2,000 as burn-in, yielding a maxdiff of
0.0884638.
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Figure S18 -- Maximum Likelihood cladogram derived from “Ecdysozoa” matrix E,
inferred in IQ-tree v1.6.2 under the PMSF approximation of the C60+LG+FO+R4 model,
with 1000 UFboot2 replicates corrected with NNI.
Figure S19 -- Cladogram from posterior consensus summary of CAT+GTR+Γ4 amino
acid analysis of “Non-Bilateria” matrix N, the same as presented in Figure 5A. Two
chains were used to form this consensus, sampling every 10 generations and
discarding the first 5,000 as burn-in, yielding a maxdiff of 0.078028.
Figure S20 -- Cladogram from posterior consensus summary of CAT+GTR+Γ4
Dayhoff-6 recoded analysis of “Non-Bilateria” matrix N, the same as presented in
Figure 5B. Two chains were used to form this consensus, sampling every 10
generations and discarding the first 5,000 as burn-in, yielding a maxdiff of 0.239156.
Figure S21 -- Cladogram from posterior consensus summary of CAT+GTR+Γ4 amino
acid analysis of “Non-Bilateria” matrix N’, the same as presented in Figure 5C. Two
chains were used to form this consensus, sampling every 10 generations and
discarding the first 5,000 as burn-in, yielding a maxdiff of 0.0944149.
Figure S22 -- Cladogram from posterior consensus summary of CAT+GTR+Γ4
Dayhoff-6 recoded analysis of “Non-Bilateria” matrix N’, the same as presented in
Figure 5D. Two chains were used to form this consensus, sampling every 10
generations and discarding the first 2,000 as burn-in, yielding a maxdiff of 0.06295.
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Figure S23 – Cladogram from ML analysis of “Non-Bilateria” matrix N, inferred in IQ-
tree v1.6.2 under the PMSF approximation of the C60+LG+FO+R4 model, with 1000
UFboot2 replicates corrected with NNI.
Figure S24 – Cladogram from ML analysis of “Non-Bilateria” matrix N’, inferred in IQ-
tree v1.6.2 under the PMSF approximation of the C60+LG+FO+R4 model, with 1000
UFboot2 replicates corrected with NNI.
Table S1 – Mapping between full species name, abbreviated names used in Figures
S1-S24, assembly type, and SRA/Bioproject numbers/genome repository for raw data
source. Taxa annotated with * represent rogue taxa masked in Matrix M, those
annotated with † were deleted during the construction of Matrix M, and those
annotated with ‡ were taxa included in the orthology analysis, but which were not
included among the alignments with minimum 100 spp.
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0.3
ANNE_Diur
NEME_Peeb
PLAT_Gapp
HEMI_Cgra
PHOR_Ppsa
OUTC_Mbre
PRIA_Pcau
CHAE_Selg
MOLL_Npom
MOLL_Mlab
ACOE_Isop
OUTF_Rsol
CRAN_Drer
HEMI_Tbah
ACOE_Hmia
ROTI_Rrot
NEMA_Hcon
CRAN_Mmus
PLAT_Ecmu
ANNE_Ofus
ACOE_Eumc
MOLL_Lvpl
NEME_Buni
OUTF_Mver
HEMI_Skow
ECHI_Spur
NEME_Hiji
ENTO_Bgra
ANNE_Mbel
NEME_Cham
NEMA_Celg
OUTF_Spun
PHOR_Paus
ARTH_Etai
ARTH_Dmel
PORI_Psub
OUTI_Apar
ANNE_Spsm
BRAC_Nvcn
CRAN_Hsap
BRAC_Gpyr
PRIA_Tubi
CHAE_Spdl
PLAC_Tadh
LORI_Arlc
BRYO_Hpac
CNID_Atet
MICR_Limn
CTEN_Vmul
CNID_AdigCNID_Nvec
ARTH_Ains
CTEN_Edun
ECHI_Pmin
PORI_Pfic
CTEN_Baby
HEMI_Sbra
PORI_Cvar
PLAT_Rros
CYCL_Symb
DICY_Dicy
KINO_Pkie
PLAT_Gnos
PORI_Ccan
PRIA_Meio
ARTH_Smar
TARD_HdEd
UROC_Odio
ECHI_Olon
ANNE_Phyl
CTEN_Pbac
ACOE_Sros
PLAT_Mfus
ANNE_Pamp
MOLL_Ovul
ECHI_Hgla
MOLL_Phol
PORI_Ccla
OUTI_Falb
ENTO_Lxmt
ACOE_Diog
CRAN_Pmar
ECHI_Lvar
ECHI_Ojap
OUTF_Rall
OUTF_Rirr
MOLL_Cgig
BRYO_Bstl
CNID_Smel
ANNE_Myzo
BRAC_Ttvs
PLAT_Sman
ANNE_Ssqu
HEMI_Pbah
CRAN_Ggal
ACOE_Diol
CNID_Csow
BRYO_Mmem
ARTH_Dpul
CRAN_Lcha
XENO_XbJC
ARTH_Amel
CRAN_Cmil
UROC_Cint
PORI_Avas
ONYC_Pcap
ARTH_Limu
NEMO_Mers
ANNE_Hrob
GAST_Megd
MOLL_Lott
ANNE_Loba
PHOR_Phar
GNAT_Augn
NEMO_Flag
ECHI_Etri
CNID_Phyd
CNID_Hvul
MOLL_Svel
TARD_Mtar
XENO_Xboc
ANNE_Pfoe
MOLL_Pcus
PLAT_Pvit
KINO_Eduj
ARTH_Smim
ROTI_Avag
CYCL_SyRN
GNAT_Trik
NEMA_Pred
ENTO_PclF
NEMA_Lloa
PORI_Scil
NEME_Aaus
CNID_Gven
OUTF_Amac
NEMM_Nect
ANNE_Ncae
MOLL_Cton
ARTH_Gmar
ROTI_Egad
NEMO_Asco
PORI_Aque
ROTI_Bcal
ARTH_Fcan
BRAC_Krub
ECHI_Oech
ONYC_Pept
CEPH_Aluc
ANNE_Mjoh
ACOE_Conv
PORI_Scoa
CTEN_Mlei
NEMA_Rcul
ROTI_Mhir
ONYC_Peri
PLAT_Mlin
ARTH_Scut
ACOE_Nfus
BRAC_LanaBRAC_Hpst
ARTH_Isca
GAST_Lepi
OUTI_Cowc
MOLL_Nmna
BRYO_Fcor
OUTI_Sart
MOLL_Lrug
OUTI_Ttra
PLAT_Sleu
OUTF_Foxy
GNAT_Gnat
PORI_CelePORI_Slac
NEMA_Tmur
ARTH_Fmer
NEMA_Tspi
OUTI_Mvar
GAST_Dpdl
ARTH_Cfin
GAST_Mdas
PORI_Ocar
GAST_Dnot
ECHI_Sbri
PORI_Cnuc
BRAC_Mcdv
ANNE_Plcs
ONYC_Oope
ANNE_Ctel
PLAT_Bsem
CEPH_Bflo
ACOE_Chil
NEMA_Ppac
OUTC_Sros
PLAT_Smed
ECHI_Ajap
NEMO_Nemw
ENTO_Loxp
ANNE_Lrub
MOLL_Hphy
PORI_Ifas
100
100
100
100
75
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
75
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
S1
-
0.4
OUTF_Rall
HEMI_Sbra
NEME_Aaus
ARTH_Fcan
ARTH_Etai
ROTI_Avag
NEMO_Flag
CHAE_Selg
PRIA_Meio
ROTI_Egad
OUTI_Mvar
ARTH_Scut
BRYO_Mmem
OUTI_Cowc
ARTH_Isca
ARTH_Dpul
ANNE_Diur
ACOE_Hmia
CNID_Adig
NEMA_Ppac
NEMA_Celg
XENO_XbJC
UROC_Odio
NEMO_Mers
PORI_Cele
BRAC_Hpst
ECHI_Etri
ACOE_Isop
XENO_Xboc
CRAN_Cmil
CNID_Csow
ARTH_Smar
OUTF_Foxy
ROTI_Rrot
CRAN_Lcha
GAST_Mdas
CRAN_Drer
ONYC_Pcap
PHOR_Paus
MOLL_Nmna
MOLL_Phol
CRAN_Pmar
BRAC_Gpyr
PLAT_Smed
NEME_Peeb
NEMA_Lloa
ACOE_Chil
PLAC_Tadh
ENTO_PclF
MOLL_Lott
ECHI_Spur
PORI_Ocar
ANNE_Ncae
ONYC_Pept
ARTH_Cfin
ARTH_Ains
OUTI_Apar
MOLL_Mlab
MOLL_Lrug
PLAT_Rros
PLAT_Gapp
CEPH_Aluc
ECHI_Ajap
PORI_Psub
CNID_Atet
ARTH_Smim
OUTF_Mver
NEMO_Asco
MOLL_Pcus
ANNE_Spsm
ACOE_Sros
CTEN_Vmul
ACOE_Diog
NEMA_Pred
BRAC_Nvcn
MOLL_Svel
ONYC_Peri
MOLL_Cton
CRAN_Mmus
PHOR_Phar
GAST_Dnot
NEME_Buni
ECHI_Ojap
ECHI_Olon
ARTH_Gmar
PLAT_Ecmu
MOLL_Cgig
PLAT_Mlin
BRYO_Fcor
OUTC_Sros
ENTO_Lxmt
ROTI_Bcal
PORI_Scoa
HEMI_Tbah
KINO_Eduj
ANNE_Phyl
CTEN_Edun
PLAT_Sman
PLAT_Pvit
NEMA_Tmur
CNID_Hvul
OUTF_Spun
MOLL_Hphy
ECHI_Sbri
ECHI_Lvar
ANNE_Loba
MOLL_Lvpl
NEME_Hiji
CNID_Phyd
BRAC_Ttvs
CRAN_Hsap
CHAE_Spdl
MICR_Limn
TARD_Mtar
CYCL_Symb
ANNE_Ctel
CTEN_Mlei
CEPH_Bflo
ARTH_Amel
PORI_Aque
CTEN_Baby
ACOE_Eumc
ANNE_Ofus
TARD_HdEd
ARTH_Fmer
NEMA_Tspi
CNID_Nvec
HEMI_Cgra
OUTF_Amac
PORI_Ccan
ENTO_Bgra
NEMM_Nect
ECHI_Pmin
BRAC_Lana
PORI_Cvar
BRAC_Krub
MOLL_Ovul
PLAT_Mfus
LORI_Arlc
PORI_Cnuc
PORI_Ccla
ROTI_Mhir
ANNE_Lrub
GNAT_Trik
HEMI_Skow
PHOR_Ppsa
BRYO_Hpac
ECHI_Oech
ACOE_Conv
BRYO_Bstl
GAST_Lepi
ANNE_Pamp
NEMA_Rcul
OUTI_Ttra
PORI_Ifas
HEMI_Pbah
KINO_Pkie
ANNE_Hrob
MOLL_Npom
ENTO_Loxp
ACOE_Diol
NEMA_Hcon
NEME_Cham
OUTI_Falb
PLAT_Gnos
CNID_Smel
ANNE_Pfoe
ANNE_Mjoh
PLAT_Bsem
OUTI_Sart
ARTH_Limu
GNAT_Gnat
GAST_Dpdl
ANNE_Plcs
ANNE_Myzo
GNAT_Augn
ANNE_Mbel
CRAN_Ggal
GAST_Megd
PORI_Pfic
OUTC_Mbre
ARTH_Dmel
NEMO_Nemw
PORI_Scil
PORI_Slac
OUTF_Rsol
ECHI_Hgla
ONYC_Oope
CTEN_Pbac
UROC_Cint
PRIA_Pcau
CNID_Gven
ACOE_Nfus
PORI_Avas
PLAT_Sleu
100
100
100
100
100
100
93
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
92
36
100
100
100
91
100
100
100
100
100
100
100
100
100
100
100
100
97
100
100
100
100
100
64
100
100
100
100
100
97
100
100
64
100100
27
100
100
100
100
100
61
100
100
100
100
100
100
71
99
100
100
100
100
100
100
100
100
27
100
100100
100
99
100
100
94
100
100
100
100
99
100
14
100
100
100
100
100
100
100
95
98
100
100
100
100
100
100
96
100
62
93
100
100
100
100
100
100
100
88
100
100
100
100 100
100
100
100
100
100
100
100
100
100
67
100
100
100
100
100
100
100
59
1
100
100
100
100
9
100
100
100
100
100
100
100
98
35
100
100
100
100
67
100
100
83
100
100
100
100
100
59
100
100
23
100
100
100
100
62
100
100
100
100
100
100
100
S2
-
0.4
PLAT_Smed
ECHI_Sbri
NEMA_Lloa
PHOR_Ppsa
BRAC_Hpst
ANNE_Pfoe
MOLL_Npom
NEMA_Hcon
HEMI_Skow
PRIA_Meio
ACOE_Hmia
NEMA_Tmur
BRAC_LanaBRAC_Gpyr
ARTH_Etai
MOLL_Lott
ACOE_Chil
XENO_XbJC
ROTI_Rrot
GAST_Lepi
CRAN_Lcha
CNID_Hvul
ACOE_Conv
CNID_Gven
ANNE_Pamp
PLAT_Gnos
ACOE_Isop
PLAT_Rros
PORI_Slac
ARTH_Smim
NEMO_Nemw
MOLL_Nmna
ANNE_Mjoh
UROC_Cint
ARTH_Dmel
HEMI_Tbah
PLAT_Mlin
MOLL_Hphy
ROTI_Mhir
GAST_Mdas
ENTO_BgraCYCL_Symb
ROTI_Avag
PORI_Aque
NEMA_Pred
PORI_Cele
ARTH_Fcan
PORI_Cvar
PHOR_Phar
MOLL_Lrug
BRYO_Mmem
BRYO_Fcor
CNID_Phyd
ARTH_Dpul
MOLL_Mlab
CRAN_Drer
MOLL_Cgig
NEMO_Mers
PLAT_Bsem
PLAT_Mfus
ECHI_Etri
NEME_Aaus
PORI_Ccan
OUTC_Mbre
ARTH_Ains
CTEN_Mlei
CNID_Csow
MOLL_Ovul
CTEN_Edun
HEMI_Pbah
ECHI_Oech
ARTH_Gmar
PLAT_Ecmu
PORI_Psub
ECHI_Spur
ANNE_Mbel
GAST_Dnot
ACOE_Diog
GAST_Dpdl
OUTC_Sros
MOLL_Lvpl
MOLL_Svel
GNAT_Trik
NEMA_Ppac
ONYC_Oope
ECHI_Olon
PHOR_Paus
CRAN_Ggal
PLAT_Gapp
CEPH_Bflo
ARTH_Isca
ECHI_Pmin
BRYO_Hpac
CTEN_Baby
CNID_Smel
GAST_Megd
ONYC_Peri
NEMA_Tspi
CNID_Atet
CNID_Nvec
CRAN_Mmus
PLAT_Pvit
ROTI_Bcal
ANNE_Phyl
CTEN_Pbac
CHAE_Spdl
BRAC_TtvsBRAC_Krub
ARTH_Limu
ECHI_Lvar
ANNE_Ncae
PLAT_Sleu
ECHI_Ojap
GNAT_Augn
ARTH_Amel
ACOE_Nfus
ANNE_Lrub
MICR_Limn
ECHI_Ajap
NEME_Hiji
CHAE_Selg
PLAC_Tadh
TARD_Mtar
NEMO_Asco
NEME_Buni
NEME_Cham
CRAN_Hsap
ACOE_Sros
ANNE_Plcs
PORI_Scoa
ONYC_Pept
MOLL_Phol
NEMA_Rcul
PORI_Pfic
ENTO_Loxp
PORI_Cnuc
CEPH_Aluc
MOLL_Cton
ARTH_Fmer
HEMI_Cgra
CNID_Adig
NEMA_Celg
ACOE_Diol
CRAN_Pmar
PORI_Ocar
PRIA_Pcau
BRAC_Nvcn
NEME_Peeb
PORI_Ifas
ANNE_Ctel
PORI_Scil
HEMI_Sbra
ANNE_Hrob
ECHI_Hgla
MOLL_Pcus
CTEN_Vmul
ARTH_Cfin
BRYO_Bstl
PLAT_Sman
TARD_HdEd
ONYC_Pcap
CRAN_Cmil
ARTH_Smar
1
1
1
0.99
1
1
1
1
1
1
0.84
0.62
1
1
1
1
1
1
1
1
1
0.97
1
1
1
1
1
1
0.99
1
1
1
1
1
1
1
11
1
1
1
1
1
1
1
1
1
1
1
1
1
11
1
0.87
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
11
1
1
1
1
1
1
1
0.63
1
0.95
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0.78
1
0.99
1
1
1
1
1
0.96
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0.99
1
1
1
1
1
1
1
1
1
1
1
0.94
1
1
1
0.97
1
1
1
1
1
1
0.99
1
1
1
1
1
1
1
0.96
0.99
1
1
S3
-
0.4
CNID_Phyd
PHOR_Paus
NEMA_Tmur
ARTH_Isca
ACOE_Nfus
GNAT_Augn
BRYO_Bstl
BRAC_Ttvs
GAST_Lepi
BRAC_Lana
XENO_XbJC
PORI_Cvar
ECHI_Etri
PORI_Ccan
OUTC_Mbre
CRAN_Cmil
NEME_Peeb
ONYC_Oope
CRAN_Hsap
CNID_Adig
ANNE_Phyl
ARTH_Etai
ANNE_Mbel
PORI_Aque
ARTH_Ains
ACOE_Hmia
NEMA_Pred
ANNE_Ncae
TARD_Mtar
NEMM_Nect
PLAT_Rros
NEMA_Ppac
ACOE_Conv
PORI_Pfic
PLAT_Pvit
NEMA_Hcon
ECHI_Ajap
ARTH_Fcan
HEMI_Cgra
CRAN_Pmar
ANNE_Mjoh
MOLL_Cton
PORI_Ocar
PLAT_Mfus
ENTO_Loxp
CTEN_Edun
ANNE_Ctel
MOLL_Lvpl
PLAT_Smed
BRYO_Hpac
MOLL_Lott
HEMI_Skow
CTEN_Baby
ENTO_Bgra
BRAC_Nvcn
ANNE_Pamp
ONYC_Pcap
CNID_Smel
CTEN_Pbac
OUTC_Sros
GAST_Dnot
ARTH_Smim
ANNE_Plcs
TARD_HdEd
MICR_Limn
ANNE_Lrub
MOLL_Hphy
ACOE_Isop
PLAC_Tadh
ANNE_Pfoe
NEME_Cham
MOLL_Nmna
ARTH_Smar
CRAN_Lcha
ECHI_Lvar
ECHI_Hgla
BRYO_Mmem
CNID_Atet
HEMI_Pbah
CNID_Hvul
CRAN_Drer
PORI_Scoa
GNAT_Trik
PHOR_Ppsa
PHOR_Phar
ROTI_Mhir
PRIA_Pcau
PLAT_Ecmu
CEPH_Aluc
GAST_Mdas
PRIA_Meio
PORI_Psub
CNID_Csow
MOLL_Lrug
CRAN_Ggal
ECHI_Olon
ARTH_Dpul
ARTH_Gmar
NEME_Hiji
HEMI_Sbra
MOLL_Ovul
PLAT_Sleu
ACOE_Diol
NEMA_Tspi
BRAC_Krub
PLAT_Gapp
CHAE_Selg
PORI_Ifas
MOLL_Phol
CEPH_Bflo
GAST_Dpdl
CTEN_Vmul
ANNE_Hrob
NEMO_Mers
PLAT_Mlin
ACOE_Chil
MOLL_Mlab
PLAT_Sman
PORI_Scil
NEMO_Asco
CNID_Nvec
ARTH_Dmel
ECHI_Sbri
MOLL_Npom
BRAC_Gpyr
PORI_Cnuc
KINO_Pkie
UROC_Cint
ACOE_Sros
CNID_Gven
PORI_Cele
ARTH_Cfin
ARTH_Limu
BRAC_Hpst
PLAT_Bsem
NEMA_Rcul
ROTI_RrotROTI_Avag
NEME_Buni
CYCL_Symb
ARTH_Fmer
LORI_Arlc
HEMI_Tbah
MOLL_Cgig
KINO_Eduj
MOLL_Svel
NEME_Aaus
CHAE_Spdl
ECHI_Oech
GAST_Megd
ECHI_Ojap
MOLL_Pcus
ECHI_Pmin
ARTH_Amel
CRAN_Mmus
CTEN_Mlei
NEMA_Celg
PORI_Slac
PLAT_Gnos
NEMO_Nemw
ECHI_Spur
BRYO_Fcor
ACOE_Diog
ROTI_Bcal
ONYC_Peri
ONYC_Pept
NEMA_Lloa
1
1
1
1
1
1
0.99
1
0.82
1
1
1
0.97
1
1
1 1
0.86
0.78
1
1
1
1
1
1
1
0.85
1
1
0.79
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0.96
1
1
1
1
1
1
1
1
1
1
1
0.94
0.95
0.62
1
0.63
1
1
1
1
1
1
1
1
1
0.74
1
1
1
0.99
1
1
1
0.99
0.99
1
1
1
1
1
0.95
1
1
1
1
1
1
1
1
1
1
0.96
1
1
1
1
1
1
1
0.99
1
1
0.84
0.94
1
1
1
1
0.97
1
1
1
1
1
1
1
1
1
1
1
0.99
1
1
1
1
1
0.72
1
1
0.87
1
1
1
S4
-
0.2
CNID_Atet
KINO_Pkie
CTEN_Edun
ARTH_Isca
ROTI_Avag
MOLL_Npom
PRIA_Pcau
ANNE_Pamp
ONYC_Peri
MOLL_Hphy
CNID_Csow
BRAC_Ttvs
ENTO_Bgra
PLAT_Ecmu
NEME_Aaus
PORI_Aque
NEME_Cham
PORI_Psub
BRYO_Hpac
ANNE_Hrob
PLAT_Bsem
ARTH_Amel
HEMI_Pbah
ARTH_Dmel
CNID_Smel
BRAC_Nvcn
PLAT_Mfus
CNID_Phyd
CTEN_Vmul
CEPH_Aluc
ECHI_Lvar
PORI_Slac
ANNE_Plcs
PLAT_Gapp
ACOE_Hmia
ONYC_Pept
BRAC_Krub
LORI_Arlc
ONYC_Pcap
NEMA_Lloa
CHAE_Spdl
CNID_Hvul
MOLL_Cton
NEMA_Rcul
ARTH_Fmer
PLAT_Sman
ANNE_Lrub
CHAE_Selg
ECHI_Etri
ACOE_Sros
PLAT_Rros
ANNE_Mbel
ARTH_Cfin
PORI_ScoaCTEN_Baby
ARTH_Ains
MOLL_Pcus
MOLL_Svel
GAST_Megd
PORI_Ocar
NEME_Hiji
ARTH_Gmar
PORI_Ifas
CTEN_Pbac
TARD_HdEd
OUTC_Mbre
KINO_Eduj
CNID_Gven
BRAC_Lana
ECHI_Olon
ARTH_Fcan
BRAC_Hpst
ONYC_Oope
NEMO_Mers
MOLL_Lrug
CRAN_Cmil
BRYO_Fcor
ANNE_Ctel
CNID_Nvec
GAST_Dnot
CRAN_Drer
NEMM_Nect
ARTH_Etai
ECHI_Hgla
CTEN_Mlei
NEMA_Tspi
ECHI_Sbri
HEMI_Sbra
PLAC_Tadh
PORI_Ccan
MOLL_Ovul
ARTH_Limu
ANNE_Mjoh
MOLL_Nmna
CRAN_Pmar
PORI_Cele
BRYO_Bstl
GNAT_Augn
MOLL_Lvpl
GAST_Lepi
PORI_Scil
CRAN_Mmus
MICR_Limn
ACOE_Conv
CNID_Adig
ACOE_Diol
PORI_Cnuc
ENTO_Loxp
PLAT_Mlin
MOLL_Cgig
ROTI_Bcal
CRAN_Hsap
ANNE_Ncae
UROC_Cint
OUTC_Sros
ACOE_Isop
NEMO_Asco
ARTH_Dpul
CEPH_Bflo
HEMI_Cgra
HEMI_Tbah
GNAT_Trik
PLAT_Gnos
NEMA_Celg
ARTH_Smar
ROTI_Rrot
PLAT_Pvit
BRYO_Mmem
GAST_Mdas
BRAC_Gpyr
CRAN_Lcha
ECHI_Ojap
MOLL_Phol
ECHI_Ajap
ACOE_ChilACOE_Nfus
ECHI_Pmin
PORI_Pfic
MOLL_Mlab
NEMA_Ppac
CYCL_Symb
PLAT_Sleu
PORI_Cvar
PLAT_Smed
PRIA_Meio
NEME_Peeb
CRAN_Ggal
ARTH_Smim
MOLL_Lott
NEME_Buni
ANNE_Phyl
XENO_XbJC
PHOR_Paus
NEMA_Tmur
ROTI_Mhir
ACOE_Diog
PHOR_Ppsa
NEMA_Pred
NEMO_Nemw
ANNE_Pfoe
GAST_Dpdl
NEMA_Hcon
TARD_Mtar
PHOR_Phar
ECHI_Oech
HEMI_Skow
ECHI_Spur
100
100
100
100
100
100
100
100
100
100
49
100
100
100
100
100
100
100
100
100
100
100
84
100
100
100
100
100
94
76
42
69100
100
91
100
100
100
100
100
99
100
94
68
57
68
100
100
100
100
100
67
33
100
98
100
100
73
99
100
100
100
66
100
100
99
86
100
100
61
100
100
51
56
100
100
100
100
100
100
68
100
100
27
100
100
100
100
49
100
100
100
100
100
100
100
100
91
100
100100
94
100
100
22
100
100
97
95
52
91
100
72
100
45
100
100
100
100
100
100
100
100
100
100
70
48
100
68
100
100
100
58
100
8190
78
90
100
100
100
100
100
100
30
72
88
100
100
100
100
51
100
100
100
87
100
100
100
52
100
66
96
100
92
S5
-
0.4
BRAC_Krub
XENO_XbJC
CYCL_SymbPLAT_Sman
GAST_Megd
LORI_Arlc
TARD_Mtar
NEME_Aaus
GNAT_Augn
ROTI_Bcal
ANNE_Hrob
PORI_Ocar
ECHI_Oech
NEMA_Pred
PLAT_Gnos
CTEN_Pbac
MOLL_Hphy
KINO_Pkie
NEME_Buni
PLAT_Smed
NEMA_Tmur
ECHI_Olon
ACOE_Isop
CRAN_Ggal
CNID_Hvul
ANNE_Phyl
ARTH_Gmar
NEMA_Tspi
PRIA_Pcau
ARTH_Cfin
NEMM_Nect
CNID_Nvec
PORI_Cvar
MOLL_Phol
CRAN_Drer
ARTH_Smim
CNID_Smel
ACOE_Hmia
PRIA_Meio
HEMI_Sbra
ANNE_Ctel
PORI_Aque
ONYC_Oope
CNID_Gven
MOLL_Lott
ARTH_Etai
BRAC_Gpyr
ECHI_Ojap
ACOE_Diol
PHOR_Phar
CRAN_Lcha
PLAT_Gapp
OUTC_Mbre
HEMI_Skow
GNAT_Trik
CRAN_Mmus
KINO_Eduj
CHAE_Spdl
MOLL_Cton
GAST_Mdas
PLAT_Pvit
CRAN_Pmar
PORI_Pfic
OUTC_Sros
PLAC_Tadh
BRYO_Hpac
ANNE_Ncae
ARTH_Amel
CTEN_Edun
MOLL_Npom
NEMO_Mers
PHOR_Paus
ONYC_Pcap
MOLL_Nmna
NEMA_Hcon
BRAC_Hpst
ECHI_Sbri
ANNE_Pamp
PORI_Cele
PLAT_Ecmu
ROTI_Rrot
TARD_HdEd
ACOE_Sros
ARTH_Isca
GAST_Dpdl
PLAT_Mfus
PLAT_Mlin
ACOE_Chil
PORI_Slac
CEPH_Aluc
ONYC_Pept
ACOE_Conv
BRAC_Nvcn
ANNE_Plcs
MOLL_Svel
NEMO_Nemw
NEMA_Ppac
BRYO_Fcor
CHAE_Selg
NEMA_Celg
ECHI_Spur
CEPH_Bflo
PORI_Psub
PORI_Scil
ACOE_Nfus
MOLL_Lrug
BRYO_Mmem
ARTH_Ains
PLAT_Rros
PLAT_Bsem
NEMA_Rcul
ONYC_Peri
ARTH_Fmer
CRAN_Hsap
ANNE_Lrub
BRYO_Bstl
UROC_Cint
ARTH_Fcan
MOLL_Mlab
ARTH_Dpul
CNID_Csow
CNID_Phyd
HEMI_TbahHEMI_Pbah
ANNE_Mbel
ACOE_Diog
PHOR_Ppsa
ROTI_Mhir
CTEN_MleiCTEN_Baby
ARTH_Dmel
ECHI_Lvar
ANNE_Pfoe
BRAC_Ttvs
CNID_Atet
MOLL_Ovul
PORI_Ccan
ROTI_Avag
HEMI_Cgra
ENTO_Loxp
ECHI_Hgla
MOLL_Pcus
CNID_Adig
NEMO_Asco
ANNE_Mjoh
PORI_Cnuc
ENTO_Bgra
PORI_Ifas
BRAC_Lana
ARTH_Smar
MOLL_Lvpl
GAST_Lepi
NEME_Cham
NEME_Peeb
ARTH_Limu
MICR_Limn
CRAN_Cmil
CTEN_Vmul
ECHI_Pmin
PLAT_Sleu
ECHI_Etri
ECHI_Ajap
PORI_Scoa
MOLL_Cgig
NEME_Hiji
GAST_Dnot
NEMA_Lloa
0.84
1
1
0.99
1
1
0.95
1
1
1
1
1
1
1
1
1
0.62
1
0.99
0.67
1
1
1
0.87
0.89
1
1
0.83
1
1
1
0.89
1
1
1
1
1
1
0.98
1
1
1
1
11
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0.62
1
1
1
1
1
1
0.97
1
1
1
1
1
0.89
0.87
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0.99
1
1
1
1
1
1
1
1
0.91
1
1
1
1
1
1
0.98
1
1
1
1
0.95
1
1
1
1
0.65
1
1
1
0.94
1
1
1
1
0.94
1
1
1
1
1
1
1
0.98
1
1
1
1
1
1
1
0.89
1
1
1
1
1
1
1
1
S6
-
ANNE_NcaeANNE_Mbel
PLAT_Rros
PRIA_Pcau
NEMA_Rcul
PLAT_Gnos
ECHI_Olon
ANNE_Hrob
NEMA_Ppac
MOLL_Cton
NEMO_Asco
OUTC_Sros
MOLL_Lvpl
BRYO_Fcor
GAST_Mdas
ECHI_Spur
PHOR_Phar
NEMO_Mers
PLAT_Mlin
PHOR_Ppsa
BRYO_Bstl
CTEN_Edun
CHAE_Spdl
ECHI_Etri
ACOE_Diog
CNID_Gven
CNID_Hvul
ECHI_Ajap
CNID_Smel
BRYO_Hpac
ARTH_Dmel
ARTH_Smar
BRYO_Mmem
ARTH_Etai
PORI_Cvar
PLAT_Bsem
PORI_Ccan
CNID_Nvec
ECHI_OjapHEMI_Tbah
MOLL_Lott
PLAT_SmanPLAT_Ecmu
PRIA_Meio
CRAN_Ggal
GAST_Dnot
TARD_HdEd
ONYC_Oope
ACOE_Nfus
HEMI_Skow
PORI_Cnuc
ANNE_Lrub
PORI_Slac
MOLL_Mlab
PLAT_Sleu
ANNE_Mjoh
PORI_Cele
PORI_Ocar
CYCL_Symb
CRAN_Lcha
ACOE_Isop
ROTI_Bcal
CTEN_Pbac
ROTI_RrotROTI_Avag
MOLL_Ovul
MOLL_Phol
NEMA_Celg
PORI_Pfic
ECHI_Pmin
ANNE_Plcs
ARTH_Amel
ENTO_Loxp
CRAN_Pmar
ROTI_Mhir
CNID_Phyd
NEME_Aaus
ACOE_Sros
ARTH_Ains
MICR_Limn
PLAT_Smed
BRAC_Lana
ANNE_Pfoe
NEMA_Tmur
ECHI_Hgla
CEPH_Bflo
NEMA_Hcon
NEMA_Lloa
CTEN_Baby
HEMI_Sbra
MOLL_Nmna
PHOR_Paus
GAST_Megd
CEPH_Aluc
HEMI_CgraCRAN_Mmus
XENO_XbJC
ACOE_Chil
BRAC_Nvcn
ARTH_Fcan
PORI_Psub
BRAC_Ttvs
ANNE_Pamp
MOLL_Pcus
ARTH_Cfin
ARTH_Limu
MOLL_Lrug
CRAN_Hsap
CTEN_MleiCTEN_Vmul
GAST_Lepi
CRAN_Cmil
CNID_Adig
ARTH_Fmer
BRAC_Gpyr
ONYC_Peri
PORI_Ifas
CNID_Csow
ECHI_Sbri
ECHI_Lvar
ONYC_Pcap
CRAN_Drer
PORI_Aque
MOLL_Hphy
ACOE_Hmia
BRAC_Krub
MOLL_Cgig
UROC_Cint
TARD_Mtar
ANNE_Phyl
BRAC_Hpst
NEMA_Pred
PLAC_Tadh
PORI_Scil
ECHI_Oech
ACOE_Diol
ONYC_Pept
NEMO_Nemw
NEME_Cham
NEME_Buni
MOLL_Svel
NEME_Peeb
NEMA_Tspi
CHAE_Selg
ACOE_Conv
HEMI_Pbah
GNAT_Trik
PLAT_Gapp
ENTO_Bgra
OUTC_Mbre
ANNE_Ctel
CNID_Atet
PORI_Scoa
PLAT_Mfus
ARTH_Gmar
GAST_Dpdl
GNAT_Augn
PLAT_Pvit
MOLL_Npom
NEME_Hiji
ARTH_Isca
1
0.71
1
1
0.92
1
1
1
1
1
1
1
1
1
1
1
0.97
1
1
1
1
1
1
1
1
0.83
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0.97
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
11
1
1
1
1
1
0.76
1
1
0.5
1
1
0.69
1
1
1
1
1
1
1
0.88
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0.91
1
1
1
1
1
1
1
1
0.87
0.95
0.9
0.92
1
1
1
1
1
1
0.99
1
1
0.86
1
1
1
1
1
1
0.96
1
1
1
1
1
1
1
1
1
1
1
0.52
1
1
1
1
1
1
1
1
1
1
1
0.87
0.96
1
S7
-
ROTI_Mhir
PRIA_Meio
NEMA_Tmur
BRYO_Mmem
PHOR_Ppsa
MOLL_Phol
ECHI_Lvar
OUTC_Sros
ACOE_Hmia
GAST_Mdas
PLAT_Gapp
ROTI_Bcal
ARTH_Etai
ACOE_Isop
PLAT_Smed
NEME_Cham
ECHI_Etri
BRAC_Gpyr
ECHI_Sbri
CNID_Nvec
ROTI_Rrot
CRAN_Lcha
MOLL_Svel
CYCL_Symb
PLAT_Mfus
ECHI_Pmin
ANNE_Pamp
BRYO_Hpac
CTEN_BabyCTEN_Mlei
CHAE_Spdl
PLAC_Tadh
ENTO_Loxp
ECHI_Oech
ARTH_Amel
ANNE_PlcsANNE_Phyl
LORI_Arlc
KINO_Eduj
CTEN_Pbac
PORI_Psub
PLAT_Mlin
ECHI_Ajap
CNID_Phyd
ECHI_Hgla
NEMA_Hcon
MOLL_Lrug
ANNE_Mjoh
BRAC_Hpst
PLAT_Bsem
CRAN_Drer
CEPH_AlucACOE_Sros
PORI_Scoa
PLAT_Rros
CNID_Gven
PLAT_Sleu
NEME_Buni
ARTH_Fmer
NEMA_Tspi
PORI_Ifas
ONYC_Oope
CEPH_Bflo
BRYO_Fcor
MICR_Limn
ARTH_Isca
HEMI_Cgra
TARD_HdEd
GAST_Dpdl
XENO_XbJC
GAST_Dnot
ONYC_Pept
CNID_Atet
MOLL_Ovul
ACOE_Conv
BRAC_Ttvs
ACOE_Chil
ECHI_Ojap
CRAN_Mmus
PORI_Scil
GNAT_Augn
MOLL_Cton
NEME_Peeb
ACOE_Diog
PORI_Slac
ECHI_Spur
PLAT_Gnos
NEMA_Ppac
MOLL_Lott
MOLL_Nmna
CHAE_Selg
CTEN_Edun
PHOR_Paus
TARD_Mtar
CNID_Csow
ECHI_Olon
ARTH_Smar
ACOE_Nfus
PRIA_Pcau
ARTH_Limu
NEMM_Nect
MOLL_Mlab
ONYC_Pcap
ACOE_Diol
MOLL_Pcus
NEMO_Nemw
ANNE_Mbel
PLAT_Sman
HEMI_Sbra
MOLL_Npom
ANNE_Ncae
MOLL_Lvpl
PHOR_Phar
BRAC_Krub
PORI_Ccan
NEMA_Pred
HEMI_Tbah
ANNE_Ctel
ARTH_Fcan
NEME_Aaus
PORI_Cvar
ANNE_Pfoe
CRAN_Pmar
HEMI_Skow
ARTH_Cfin
CRAN_Cmil
CRAN_Ggal
UROC_Cint
NEMO_Mers
ARTH_Dmel
ARTH_Ains
CNID_Smel
NEMA_Celg
BRAC_Nvcn
PORI_Pfic
CNID_Hvul
HEMI_Pbah
CTEN_Vmul
PLAT_Pvit
ONYC_Peri
BRYO_Bstl
MOLL_Hphy
BRAC_Lana
NEMA_Lloa
CNID_Adig
NEME_Hiji
ARTH_Gmar
OUTC_Mbre
ENTO_Bgra
CRAN_Hsap
GAST_Lepi
PORI_Cnuc
NEMA_Rcul
NEMO_Asco
ARTH_Smim
ROTI_Avag
PLAT_Ecmu
GAST_Megd
GNAT_Trik
PORI_Ocar
ANNE_Lrub
KINO_Pkie
PORI_Cele
MOLL_Cgig
ARTH_Dpul
PORI_Aque
ANNE_Hrob
1
1
1
0.68
1
1
0.86
1
1
1
0.9
1
1
1
1
1
1
1
0.97
1
0.99
1
1
1
0.69
1
1
1
1
0.84
1
1
1
0.85
1
1
0.661
1
1
1
1
1
0.88
1
0.99
1
1
1
1
0.95
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0.97
1
1
1
0.93
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0.51
1
1
1
0.89
0.96
0.99
1
1
1
1
1
11
0.88
1
10.53
0.81
1
1
0.65
1
0.98
1
1
1
1
1
1
1
1
1
1
0.96
1
1
1
1
1
1
1
1
1
1
1
0.91
1
0.72
1
0.75
1
0.52
S8
-
0.4
PLAT_Sman
NEME_Cham
PORI_Cele
PHOR_Paus
ANNE_Ncae
ANNE_Hrob
MOLL_Lvpl
CTEN_Pbac
GAST_Megd
ANNE_Mbel
ARTH_Cfin
ONYC_Peri
OUTC_Mbre
MOLL_Mlab
BRAC_Nvcn
CTEN_Mlei
MOLL_Npom
PLAT_Rros
GNAT_Trik
PHOR_Ppsa
NEME_Buni
PORI_Scil
NEME_Peeb
BRAC_Gpyr
PLAT_Sleu
CNID_Atet
NEME_Aaus
ARTH_Smar
PORI_Scoa
MOLL_Cgig
PLAC_Tadh
CRAN_Cmil
PLAT_Gapp
ANNE_Pfoe
PRIA_Meio
ANNE_Ctel
ACOE_Isop
TARD_HdEd
CNID_Csow
PORI_Slac
BRYO_Bstl
NEMA_Hcon
MOLL_Pcus
MOLL_Ovul
ARTH_Fcan
BRYO_Hpac
MOLL_Nmna
NEMA_Rcul
CNID_Smel
PLAT_Gnos
MOLL_Hphy
ARTH_Amel
MOLL_Phol
ENTO_Loxp
MOLL_Lrug
ACOE_Sros
CTEN_Vmul
CYCL_Symb
CRAN_Mmus
ECHI_Olon
CTEN_Edun
BRAC_Hpst
PORI_Ocar
PLAT_Smed
ANNE_Phyl
ARTH_Fmer
ONYC_Pcap
ARTH_Etai
CNID_Nvec
NEMA_Ppac
ECHI_Ojap
ACOE_Diol
ROTI_Mhir
ECHI_Oech
ARTH_Isca
GAST_Lepi
CRAN_Ggal
PLAT_Ecmu
GNAT_Augn
ECHI_Lvar
ANNE_Pamp
GAST_Mdas
ARTH_Dpul
ACOE_Diog
ANNE_Lrub
PORI_Ifas
ANNE_Mjoh
MICR_Limn
HEMI_Sbra
CHAE_Spdl
ARTH_Dmel
NEMA_Lloa
TARD_Mtar
ECHI_Ajap
PLAT_Pvit
ACOE_Chil
NEMA_Tmur
BRAC_Lana
CNID_Hvul
NEMA_Tspi
PORI_Cvar
PLAT_Bsem
NEMA_Pred
BRYO_Fcor
ANNE_Plcs
ENTO_Bgra
ARTH_Gmar
ARTH_Smim
ECHI_Sbri
ROTI_Avag
BRYO_Mmem
ECHI_Pmin
HEMI_Pbah
NEMA_Celg
CEPH_Bflo
MOLL_Cton
HEMI_Skow
GAST_Dnot
ECHI_Spur
PLAT_Mlin
PORI_Ccan
PORI_Pfic
UROC_Cint
ONYC_Pept
CRAN_Drer
CTEN_Baby
PHOR_Phar
OUTC_Sros
BRAC_Krub
NEMO_Asco
BRAC_Ttvs
CHAE_Selg
NEME_Hiji
ARTH_Limu
ACOE_Conv
NEMO_Mers
PORI_Psub
MOLL_Svel
ONYC_Oope
HEMI_Tbah
ECHI_Hgla
PORI_Cnuc
CRAN_Hsap
MOLL_Lott
GAST_Dpdl
PORI_Aque
ACOE_Nfus
ECHI_Etri
XENO_XbJC
PRIA_Pcau
ACOE_Hmia
CNID_Adig
CRAN_Pmar
CNID_Gven
CEPH_Aluc
ROTI_Rrot
ROTI_Bcal
CNID_Phyd
CRAN_Lcha
HEMI_Cgra
PLAT_Mfus
ARTH_Ains
NEMO_Nemw
1
1
1
0.84
1
1
1
1
0.99
1
1
0.91
1
1
1
1
1
1
1
1
0.99
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0.87
1
0.89
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0.64
1
1
1
0.98
1
1
1
1
1
1
1
1
1
1
0.92
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0.98
0.88
1
1
1
1
1
1
1
1
1
1
1
1
0.68
1
1
0.89
1
1
1
1
1
1
1
1
1
1
1
1
1
0.98
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
S9
-
0.2
MOLL_Npom
BRYO_Fcor
ROTI_Mhir
MOLL_Mlab
BRYO_Mmem
NEME_Buni
HEMI_Skow
ECHI_Etri
PORI_Cnuc
PORI_Scil
ANNE_Phyl
ACOE_Nfus
NEMO_Mers
PORI_Cele
GAST_Mdas
NEMM_Nect
XENO_XbJC
ANNE_Ctel
PLAT_Gnos
ROTI_Bcal
GAST_Dpdl
CRAN_Ggal
CNID_Nvec
PORI_Psub
PLAT_Rros
MOLL_Nmna
PLAT_Smed
ENTO_Loxp
CRAN_Cmil
CNID_Hvul
ANNE_Hrob
PORI_Ocar
NEME_Peeb
PLAT_Pvit
ARTH_Amel
ARTH_Ains
ACOE_Conv
ARTH_Dpul
MOLL_Ovul
CNID_Smel
GAST_Megd
PORI_Pfic
CNID_Phyd
LORI_Arlc
ONYC_Oope
ACOE_Chil
NEMA_Ppac
ECHI_Sbri
ANNE_Mjoh
ECHI_Ajap
PORI_Ifas
CYCL_Symb
ARTH_Isca
ANNE_Pfoe
PLAT_Mfus
BRAC_Hpst
CRAN_Drer
PLAT_Ecmu
NEMO_Nemw
ONYC_Peri
NEMA_Tmur
ACOE_Diog
NEMA_Pred
KINO_Eduj
ECHI_Oech
ACOE_Isop
MOLL_Hphy
HEMI_Tbah
PLAT_Mlin
NEMA_Rcul
ARTH_Etai
CTEN_Mlei
NEMA_Hcon
PRIA_Pcau
ENTO_Bgra
HEMI_Sbra
ACOE_Diol
NEMA_Lloa
MOLL_Cton
CRAN_Mmus
MOLL_Lott
PLAT_Bsem
PORI_Ccan
OUTC_Sros
PLAT_Gapp
ECHI_Spur
ANNE_Mbel
ECHI_Ojap
ARTH_Gmar
BRAC_Lana
CTEN_Pbac
CRAN_Hsap
ECHI_Pmin
ROTI_Avag
CNID_Atet
ACOE_Sros
PRIA_Meio
BRYO_Hpac
NEME_ChamBRAC_Gpyr
ECHI_Olon
ANNE_Plcs
NEME_Hiji
PLAC_Tadh
MOLL_Lrug
HEMI_Cgra
ANNE_Pamp
PHOR_Phar
PORI_Cvar
GNAT_Trik
ARTH_Fmer
ARTH_Dmel
PORI_Scoa
ECHI_Hgla
PLAT_Sleu
CHAE_Selg
CTEN_Baby
ARTH_Cfin
ONYC_Pept
BRAC_Krub
ARTH_Smar
CRAN_Lcha
CHAE_Spdl
ANNE_Ncae
CNID_Gven
OUTC_Mbre
ANNE_Lrub
PORI_Slac
PHOR_Paus
ACOE_Hmia
NEME_Aaus
UROC_Cint
CNID_Adig
PLAT_Sman
BRYO_Bstl
CEPH_Bflo
MOLL_Cgig
HEMI_Pbah
TARD_HdEdTARD_Mtar
CNID_Csow
CTEN_Vmul
BRAC_Nvcn
CEPH_Aluc
ONYC_Pcap
MOLL_Svel
ARTH_Fcan
GNAT_Augn
NEMO_Asco
MOLL_Lvpl
ECHI_Lvar
CRAN_Pmar
ARTH_Smim
NEMA_Tspi
KINO_Pkie
GAST_DnotGAST_Lepi
PORI_Aque
CTEN_Edun
BRAC_Ttvs
MOLL_PcusMOLL_Phol
MICR_Limn
PHOR_Ppsa
ROTI_Rrot
ARTH_Limu
NEMA_Celg
88
100
75
100
100
100
85
100
100
100
73
100
100
100
74
97
99
100
100
19
100
100
97
78
100
100
100
100
100
77
100
67
100
79
100
100
49
100
100
100
100
79
90
100
100
100
100
91
91
100
88
100
94
100
100
100
100
85
78
100
100
12
100
100
100
100
100
100
98
89
100
100
100
100
100
57
100
93
100
100
100
99
93
100
58
49
100
100
45
100
30
100
53
95
100
100
100
100
100
65
100
99
97
100
63
100
96
100
44
100
100
99
84
100
100
100
99
100
100
100
71
100
100
100
100
100
100
88
100
100100
100
100
100
100
100
82
100
100
100
100
100
100
100
100
100
72
100
100
100
100
100
100
100
100
100
70
100
100
100
15
87
100
100
100
S10
-
0.4
PLAT_Pvit
GAST_DnotROTI_Mhir
ROTI_Rrot
BRAC_Lana
MOLL_Lvpl
PLAT_Sleu
PLAT_Smed
ENTO_PclF
ANNE_Pfoe
NEME_Peeb
PHOR_Paus
ROTI_Egad
MICR_Limn
BRYO_Fcor
ANNE_Ssqu
ANNE_Myzo
GAST_Mdas
CHAE_Spdl
BRYO_Bstl
PLAT_SmanPLAT_Ecmu
PLAT_Mlin
ANNE_Mbel
ANNE_Loba
ANNE_Plcs
MOLL_Npom
GAST_Dpdl
NEME_Aaus
PLAT_Gapp
BRAC_Ttvs
ANNE_Phyl
ANNE_Hrob
BRAC_Hpst
MOLL_Mlab
GAST_Lepi
PLAT_Gnos
ANNE_Ofus
ANNE_Ctel
BRAC_Mcdv
ENTO_Bgra
MOLL_Svel
GAST_Megd
GNAT_Trik
ANNE_Pamp
PHOR_Ppsa
CHAE_Selg
MOLL_Cton
BRAC_Nvcn
MOLL_Lott
NEME_Hiji
BRYO_Hpac
MOLL_Phol
ANNE_Ncae
BRAC_Krub
PLAT_Rros
ANNE_Diur
ANNE_Mjoh
MOLL_Lrug
CYCL_Symb
PHOR_Phar
MOLL_Cgig
GNAT_Augn
ENTO_Lxmt
NEME_Cham
ROTI_Avag
MOLL_Hphy
PLAT_Mfus
NEME_Buni
BRYO_Mmem
MOLL_Ovul
ROTI_Bcal
GNAT_Gnat
MOLL_Pcus
ENTO_Loxp
BRAC_Gpyr
PLAT_Bsem
ANNE_Spsm
ANNE_Lrub
MOLL_Nmna
0.92
0.88
0.89
1
1
0.98
1
1
0.97
0.9
1
1
0.94
0.78
0.99
1
0.66
1
1
0.98
1
0.99
1
0.79
0.98
1
1
0.99
0.95
1
1
0.98
0.95
0.99
1
0.9
0.99
0.98
0.85
1
0.74
0.98
0.52
0.99
0.68
1
0.84
0.91
0.97
1
0.97
1
0.8
1
1
0.78
0.96
0.86
1
0.9
0.89
1
1
1
0.86
0.96
0.99
1
0.52
0.63
1
1
1
0.98
0.99
1
S11
-
0.4
MOLL_Phol
PHOR_Phar
GAST_Dpdl
BRYO_Hpac
ROTI_Bcal
ANNE_Lrub
ANNE_Phyl
GAST_Lepi
GAST_Mdas
MOLL_LottMOLL_HphyMOLL_Mlab
ANNE_Pfoe
ENTO_Lxmt
PLAT_Mfus
GNAT_Augn
MOLL_Cgig
GNAT_Trik
PLAT_Gnos
ANNE_Diur
GNAT_Gnat
GAST_Megd
MOLL_Lvpl
ROTI_Mhir
CHAE_Selg
BRAC_Gpyr
PLAT_Bsem
ANNE_Ncae
BRYO_Fcor
MOLL_Npom
NEME_Aaus
BRYO_Mmem
PLAT_Sman
ROTI_Avag
MOLL_Cton
PHOR_Paus
ENTO_PclF
PLAT_Smed
ANNE_OfusANNE_Mjoh
PLAT_Ecmu
ROTI_Egad
ANNE_Pamp
MOLL_Pcus
ANNE_Plcs
MICR_Limn
ROTI_Rrot
MOLL_Nmna
PHOR_Ppsa
BRAC_Krub
ANNE_Ctel
PLAT_Rros
ANNE_Spsm
ANNE_Mbel
NEME_Cham
ENTO_BgraCYCL_Symb
PLAT_Gapp
NEME_BuniNEME_Hiji
MOLL_Lrug
PLAT_Sleu
BRYO_Bstl
BRAC_Lana
MOLL_Svel
PLAT_Pvit
CHAE_Spdl
BRAC_Nvcn
ENTO_Loxp
NEME_Peeb
MOLL_Ovul
PLAT_Mlin
GAST_Dnot
ANNE_Hrob
ANNE_Myzo
BRAC_Ttvs
BRAC_Hpst
ANNE_Loba
1
1
1
0.91
1
1
1
1
1
1
0.92
1
1
0.99
1
1
1
1
1
1
1
1
1
1
1
0.89
1
1
1
1
1
1
1
1
1
1
11
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0.93
0.94
0.98
1
1
1
1
1
1
1
1
0.99
0.92
1
1
1
0.92
S12
-
0.2
PHOR_Ppsa
ENTO_Loxp
ANNE_Mbel
MOLL_Mlab
BRAC_Hpst
ROTI_Rrot
ANNE_Ncae
PLAT_PvitPLAT_Gapp
PLAT_Bsem
ANNE_Plcs
MOLL_Cton
GNAT_Gnat
PHOR_Paus
ROTI_Egad
MOLL_Lott
ANNE_Hrob
GAST_Megd
ANNE_Myzo
NEME_Aaus
MOLL_Hphy
BRAC_Ttvs
ANNE_Mjoh
ANNE_Ctel
BRAC_Gpyr
ANNE_Diur
NEME_Hiji
CYCL_Symb
BRAC_Lana
ANNE_Pfoe
MOLL_Npom
PLAT_Mfus
NEME_Peeb
CHAE_Selg
PLAT_Rros
MOLL_Pcus
ROTI_Avag
PLAT_Mlin
BRYO_Hpac
BRAC_Nvcn
CHAE_Spdl
ANNE_Loba
PLAT_Ecmu
GAST_Lepi
ROTI_Bcal
ENTO_Lxmt
ANNE_Lrub
ANNE_Spsm
ANNE_PhylANNE_Pamp
MOLL_Phol
GAST_Dpdl
MOLL_Lvpl
BRYO_Mmem
PHOR_Phar
MOLL_Svel
GAST_Dnot
PLAT_Smed
ANNE_Ofus
PLAT_Gnos
MOLL_Cgig
ENTO_PclF
GNAT_Augn
BRAC_Krub
BRYO_BstlBRYO_Fcor
PLAT_Sleu
ENTO_Bgra
MOLL_Ovul
MOLL_Nmna
GNAT_Trik
NEME_Buni
PLAT_Sman
ROTI_Mhir
MOLL_Lrug
MICR_Limn
GAST_Mdas
NEME_Cham
1
1
1
1
1
1
1
1
1
0.97
1
1
1
0.53
1
1
0.97
1
0.55
1
0.74
0.92
0.86
1
0.87
0.89
0.79
1
1
1
1
0.99
1
0.87
1
0.56 1
0.73
1
1
1
1
1
1
1
1
1
1
1
0.98
1
1
1
0.91
1
1
1
1
1
1
1
1
1
1
0.99
1
1
0.84
0.761
0.83
1
0.99
1
1
1
S13
-
0.2
MOLL_Cgig
NEME_Cham
ANNE_Ofus
GAST_Mdas
ANNE_Mjoh
MOLL_Cton
BRAC_Gpyr
MOLL_Lrug
NEME_Aaus
ENTO_Bgra
ANNE_Hrob
MOLL_Mlab
ENTO_Loxp
ANNE_Diur
ANNE_Spsm
BRAC_Mcdv
ANNE_Lrub
ENTO_Lxmt
ANNE_Pamp
BRYO_Mmem
PLAT_Mlin
CHAE_Selg
PLAT_Pvit
ANNE_Loba
PLAT_Bsem
BRAC_Krub
PLAT_Sman
PLAT_Gapp
NEME_Hiji
MOLL_Phol
CYCL_SymbPLAT_Ecmu
PLAT_Smed
GAST_Dnot
PHOR_Paus
MOLL_Npom
BRAC_Lana
BRYO_Bstl
PLAT_Gnos
PLAT_Sleu
ANNE_Plcs
ANNE_MbelANNE_Ncae
GNAT_Trik
MICR_Limn
PHOR_Ppsa
GNAT_Gnat
GAST_Dpdl
GAST_Lepi
ANNE_Phyl
PLAT_Rros
ROTI_Avag
BRYO_Hpac
BRAC_Hpst
NEME_Buni
PHOR_Phar
MOLL_Ovul
MOLL_Lott
ANNE_Ctel
ROTI_Bcal
MOLL_Lvpl
MOLL_Nmna
ANNE_Ssqu
BRAC_Ttvs
BRAC_Nvcn
PLAT_Mfus
ANNE_Pfoe
NEME_Peeb
BRYO_Fcor
MOLL_Svel
ROTI_Rrot
MOLL_Pcus
MOLL_Hphy
GAST_Megd
ROTI_Egad
ANNE_Myzo
CHAE_Spdl
ROTI_Mhir
GNAT_Augn
ENTO_PclF
100
100
99
21
47
55
100
100
100
53
100
24
100
14
100100
98
100
19
100
73
100
33
100
65
70
100
81
100
100
100100
100
100
100
100
100
100
59
100100
94
73
100
100
76
100
85
50
100
98
91
100
100
100
100
100
100
100
100
100
73
99
100
94
100
97
18
100
100
100
92
35
100
86
61
77
35
S14
-
0.3
TARD_HdEd
ARTH_Fcan
GNAT_Trik
ARTH_Isca
NEMA_Rcul
GAST_Dnot
ARTH_Scut
ONYC_Pcap
BRAC_Ttvs
ARTH_Cfin
ARTH_Etai
ARTH_Smar
ONYC_PeriARTH_Ains
NEMM_Nect
NEMA_Lloa
KINO_Eduj
ARTH_Limu
ANNE_Ctel
PRIA_Pcau
MICR_Limn
ONYC_Oope
NEMA_HconNEMA_Celg
PRIA_Meio
NEMA_Tspi
MOLL_Lott
NEMA_Tmur
NEMA_Pred
ARTH_Fmer
TARD_Mtar
ARTH_Dpul
ARTH_Gmar
LORI_Arlc
ARTH_Dmel
NEMA_Ppac
KINO_Pkie
ARTH_Amel
PLAT_Pvit
ONYC_Pept
NEME_Cham
ARTH_Smim
PHOR_Phar
1
1
1
0.67
1
1
1
0.75
0.94
1
1
1
1
0.99
1
1
1
1
1
1
1
1
1
1
1
0.92
1
1
1
1
1
1
0.99
1
0.96
0.61
1
1
1
0.72
1S15
-
0.3
PRIA_Meio
NEME_Cham
ARTH_Amel
ARTH_Smar
MOLL_Lott
ONYC_Pcap
NEMA_Tmur
ARTH_Dmel
ARTH_Fcan
ARTH_Gmar
ARTH_Isca
PRIA_Pcau
NEMA_Celg
GAST_Dnot
ARTH_Ains
ARTH_Etai
TARD_Mtar
PHOR_Phar
MICR_Limn
ONYC_Oope
ARTH_Fmer
NEMA_Ppac
KINO_Pkie
ANNE_Ctel
GNAT_Trik
KINO_Eduj
ARTH_Cfin
PLAT_Pvit
ARTH_Limu
NEMA_Lloa
NEMA_Rcul
NEMA_Hcon
NEMA_Pred
BRAC_Ttvs
ARTH_Smim
ONYC_Peri
ONYC_Pept
LORI_Arlc
ARTH_Dpul
ARTH_Scut
TARD_HdEd
NEMA_Tspi
1
0.82
1
11
1
11
1
1
1
1
0.97
1
0.99
1
1
1
1
0.96
0.67
1
0.75
1
1
1
1
1
1
1
1
1
1
1
1
1
0.94
1
1
1
S16
-
0.2
ARTH_Etai
TARD_Mtar
ANNE_Ctel
PRIA_Pcau
TARD_HdEd
ARTH_Smar
NEMA_Celg
ARTH_Dmel
LORI_Arlc
KINO_Eduj
NEME_Cham
ARTH_Limu
NEMA_Tmur
NEMA_Hcon
ARTH_Amel
GAST_Dnot
ARTH_Smim
NEMA_Rcul
BRAC_Ttvs
ARTH_Dpul
ARTH_Scut
NEMA_Tspi
PLAT_Pvit
MICR_Limn
ARTH_Ains
PHOR_Phar
ARTH_Gmar
NEMA_Ppac
ONYC_Pept
ONYC_Pcap
NEMM_Nect
NEMA_Lloa
KINO_Pkie
ARTH_Isca
MOLL_Lott
NEMA_Pred
GNAT_Trik
ARTH_Fmer
ONYC_Peri
ARTH_Cfin
PRIA_Meio
ONYC_Oope
ARTH_Fcan
1
0.95
1
1
0.6
1
1
1
1
1
1
1
1
1
1
1
1
0.94
0.98
1
1
1
1
1
1
0.92
1
0.5
1
0.99
1
1
0.64
1
1
0.93
0.5
1
0.57
S17
-
0.2
KINO_Eduj
NEMA_Rcul
NEMA_Pred
ARTH_Dpul
TARD_Mtar
PRIA_Meio
ARTH_Dmel
ANNE_Ctel
ARTH_Smim
PHOR_Phar
NEMA_Ppac
ARTH_Etai
NEMA_Lloa
ARTH_Smar
ONYC_Oope
NEMA_Tmur
BRAC_Ttvs
ONYC_Peri
PRIA_Pcau
NEMA_Hcon
TARD_HdEd
ARTH_Fcan
NEMA_Celg
NEMM_Nect
ONYC_Pept
ARTH_Isca
ARTH_FmerARTH_Cfin
ONYC_Pcap
GNAT_Trik
ARTH_Limu
ARTH_Amel
ARTH_Scut
ARTH_Ains
NEMA_Tspi
MICR_Limn
GAST_Dnot
NEME_Cham
MOLL_Lott
ARTH_Gmar
PLAT_Pvit
KINO_Pkie
LORI_Arlc
100
100100
100
96
85
41
100
90
83
35
100
90
77
9996
100
100
95
100
100
100
100
89
100
100
84
74
100
100
100
100
100
99
100
100
50
100
100
100
58
S18
-
0.5
CNID_Hvul
PORI_Scil
CEPH_Bflo
PORI_Slac
CTEN_Mlei
CNID_Csow
OUTI_Ttra
CNID_Gven
OUTC_Mbre
PORI_Cele
OUTF_Amac
PORI_Pfic
CTEN_Pbac
OUTF_Spun
OUTC_Sros
CNID_Adig
PORI_Psub
OUTI_Apar
BRAC_Ttvs
CRAN_MmusECHI_SpurXENO_XbJC
PORI_Cnuc
OUTI_Falb
PORI_Ocar
CNID_Phyd
PLAC_Tadh
PORI_Scoa
OUTI_Mvar
ONYC_Peri
OUTI_Sart
OUTF_Mver
OUTF_Rall
PORI_Avas
CTEN_Baby
PRIA_Pcau
OUTI_Cowc
PORI_Ccla
PORI_Cvar
ANNE_Ctel
CNID_Smel
PORI_Ifas
PORI_Ccan
CTEN_Vmul
MOLL_Lott
CNID_Nvec
CNID_Atet
PORI_Aque
CTEN_Edun
OUTF_FoxyOUTF_Rsol
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0.59
1
0.98
1
1
0.96
1
1
1
1
1
0.96
1
1
11
1
0.96
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
S19
-
0.3
CTEN_Baby
PORI_Pfic
PLAC_Tadh
CTEN_Mlei
PORI_Ocar
CNID_Gven
PORI_Aque
OUTF_Mver
OUTF_Amac
CTEN_Pbac
MOLL_LottBRAC_Ttvs
CTEN_Vmul
OUTI_Mvar
CNID_Csow
PORI_Cvar
CNID_PhydCNID_Smel
PORI_Psub
PORI_Slac
ECHI_Spur
PORI_Ccan
OUTC_Mbre
PORI_Ifas
CNID_Atet
OUTI_Ttra
OUTI_Apar
ONYC_Peri
PORI_ScoaPORI_Avas
CEPH_Bflo
CNID_Adig
XENO_XbJC
OUTI_Cowc
CNID_Nvec
PORI_Scil
PORI_Cnuc
OUTC_Sros
OUTF_Rall
CTEN_Edun
PORI_Ccla
OUTF_Foxy
OUTF_Spun
OUTF_Rsol
PORI_Cele
CNID_Hvul
OUTI_Falb
CRAN_Mmus
ANNE_Ctel
OUTI_Sart
PRIA_Pcau
1
1
1
0.92
0.99
1
1
1
1
1 1
1
0.61
1
0.61
1
1
0.8
1
1
1
0.95
1
1
1
0.99
1
1
11
1
0.99
0.53
0.72
1
1
0.79
1
1
11
1
1
0.81
1
1
1
1
S20
-
0.4
CNID_Adig
OUTC_Mbre
BRAC_Ttvs
PORI_Pfic
CEPH_Bflo
OUTC_Sros
PORI_Cele
PORI_Scil
PLAC_Tadh
PORI_Slac
ANNE_Ctel
PORI_Scoa
PORI_Ocar
PORI_Aque
CTEN_Baby
CNID_Smel
CTEN_EdunCTEN_Vmul
PORI_Cvar
PRIA_Pcau
CNID_Phyd
PORI_Avas
CTEN_Pbac
CRAN_Mmus
CTEN_Mlei
PORI_Psub
CNID_Nvec
PORI_Cnuc
ONYC_Peri
CNID_Hvul
CNID_Csow
CNID_Gven
PORI_Ifas
ECHI_Spur
PORI_Ccla
MOLL_Lott
XENO_XbJC
CNID_Atet
PORI_Ccan
0.5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0.55 1
11
1
1
1
1
1
S21
-
0.2
PORI_Ccan
XENO_XbJC
OUTC_Sros
CEPH_Bflo
PORI_PficPORI_Aque
PORI_Ocar
CTEN_Vmul
PORI_Slac
PRIA_Pcau
CNID_Phyd
PORI_Avas
CTEN_Mlei
PORI_Cvar
PORI_Scil
CRAN_Mmus
PORI_Ccla
CTEN_Pbac
PORI_Cnuc
BRAC_Ttvs
CNID_Atet
PLAC_Tadh
CNID_Smel
ONYC_Peri
PORI_Scoa
CNID_Nvec
ANNE_CtelMOLL_Lott
PORI_Cele
CNID_Hvul
CTEN_Edun
ECHI_Spur
CTEN_Baby
CNID_Csow
CNID_Gven
PORI_Psub
CNID_Adig
OUTC_Mbre
PORI_Ifas
1
11
1
1
0.98
1
1
0.99
1
1
1
1
0.99
1
1
1
0.99
1
1
1
1
0.91
1
1
1
0.57
1
1
1
1
0.88
1
1
1
1
0.85
S22
-
ONYC_Peri
PORI_Ifas
OUTF_Rall
OUTF_Spun
CRAN_Mmus
OUTF_Rsol
CNID_Atet
PORI_Cvar
PORI_Avas
PORI_Ccla
CNID_Adig
ECHI_Spur
CNID_Csow
PRIA_Pcau
PORI_Psub
OUTI_Mvar
CNID_Hvul
PORI_Cnuc
OUTI_Apar
CTEN_Edun
PORI_Scoa
CNID_Smel
OUTF_Amac
CNID_Nvec
OUTI_Falb
PORI_Cele
CTEN_Mlei
PORI_Aque
PORI_Ocar
XENO_XbJC
OUTF_Foxy
PORI_Scil
PORI_Slac
OUTF_Mver
OUTI_Sart
BRAC_Ttvs
CEPH_Bflo
ANNE_Ctel
CNID_Phyd
PORI_Ccan
CTEN_Baby
OUTC_MbreOUTC_Sros
CTEN_Pbac
CNID_GvenPLAC_Tadh
OUTI_Cowc
MOLL_Lott
PORI_Pfic
CTEN_Vmul
OUTI_Ttra
100
100
100
100
96
100
100
100
100
100
100
100
100
72
100
100
100
100
100
100
97
100
86
100
95
91
100
57
100
100
100
100
100
100
100
100
100
100
100
100
74
100
100
100
10056
100
100
100
S23
-
MOLL_Lott
PORI_Scil
CEPH_BfloPRIA_Pcau
PORI_Ccan
CTEN_Pbac
CRAN_Mmus
PORI_Cnuc
BRAC_Ttvs
PORI_Cele
ANNE_Ctel
PORI_Avas
XENO_XbJC
CNID_Hvul
CNID_AdigCNID_Nvec
CTEN_Baby
CNID_Csow
PORI_Cvar
PORI_Aque
CTEN_Edun
PORI_Psub
PORI_Ocar
ONYC_Peri
PLAC_TadhCNID_Gven
PORI_Pfic
OUTC_Mbre
PORI_Ccla
OUTC_Sros
CTEN_Vmul
ECHI_Spur
PORI_Slac
CTEN_Mlei
CNID_Smel
CNID_Atet
CNID_Phyd
PORI_Scoa
PORI_Ifas
96
100
80
84
100
100
100
100
100
97
100
79
86
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
80
100
100
100
100
100
S24
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NON-BILATERIACtenophora [5] Beroe abyssicola CTEN_Baby Transcriptome (Illumina) SRR777787
Euplokamis dunlapae CTEN_Edun Transcriptome (Illumina) SRR777663Mnemiopsis leidyi CTEN_Mlei Genome PRJNA64405Pleurobrachia bachei CTEN_Pbac Genome PRJNA213480Vallicula multiformis CTEN_Vmul Transcriptome (Illumina) SRR786489
Porifera [14] Amphimedon queenslandica PORI_Aque Genome PRJNA66531Aphrocallistes vastus PORI_Avas† Transcriptome (Illumina) SRR1068281Chondrilla caribensis PORI_Cnuc Transcriptome (Illumina) https://dataverse.harvard.edu/dataverse/spotranscriptomesClathrina coriacea PORI_Ccla† Transcriptome (Illumina) SRR3417192Cliona varians PORI_Cvar Transcriptome (Illumina) SRR1391011Corticium candelabrum PORI_Ccan Transcriptome (Illumina) SRR504694Crella elegans PORI_Cele Transcriptome (Illumina) SRR648671Ircinia fasciculata PORI_Ifas Transcriptome (Illumina) https://dataverse.harvard.edu/dataverse/spotranscriptomesOscarella carmela PORI_Ocar Genome SRX386229Petrosia ficiformis PORI_Pfic Transcriptome (Illumina) SRR504688Pseudospongosorites suberitoides PORI_Psub Transcriptome (Illumina) https://dataverse.harvard.edu/dataverse/spotranscriptomesSpongilla lacustris PORI_Slac Transcriptome (Illumina) SRR1168575Sycon ciliatum PORI_Scil Transcriptome (Illumina) ERR466762Sycon coactum PORI_Scoa Transcriptome (Illumina) SRR504689,SRR504690
Placozoa [1] Trichoplax adhaerens PLAC_Tadh Genome PRJNA12874Cnidaria [8] Abylopsis tetragona CNID_Atet Transcriptome (Illumina) SRR871525
Acropora digitifera CNID_Adig Genome PRJDA67425Craspedacusta sowerbyi CNID_Csow Transcriptome (Illumina) SRR923472Gorgonia ventalina CNID_Gven Transcriptome (Illumina) SRR935083Hydra vulgaris CNID_Hvul Genome PRJNA31231Nematostella vectensis CNID_Nvec Genome PRJNA19965Polypodium hydriforme CNID_Phyd Transcriptome (Illumina) SRR1336770Stomolophus meleagris CNID_Smel Transcriptome (Illumina) SRR1168418
XENACOELOMORPHAAcoelomorpha [8] Convolutriloba macropyga ACOE_Conv Transcriptome (Illumina) SRR2681679
Diopisthoporus longitubus ACOE_Diol Transcriptome (Illumina) SRR3105704Diopisthoporus gymnopharyngeus ACOE_Diop Transcriptome (Illumina) SRR3105703Eumecynostomum macrobursalium ACOE_Eumc† Transcriptome (Illumina) SRR3105705Hofstenia miamia ACOE_Hmia Transcriptome (Illumina) SRR1208932Isodiametra pulchra ACOE_Isop Transcriptome (Illumina) SRR2681926Neochildia fusca ACOE_Nfus Transcriptome (Illumina) SRR8617822Symsagittifera roscoffensis ACOE_Sros Transcriptome (Illumina) SRR827579
Nemertodermatida [4] Ascoparia sp. BV-2015 NEMO_Asco Transcriptome (Illumina) SRR2682154Flagellophora sp. Bocas NEMO_Flag† Transcriptome (Illumina) SRR8641368Meara stichopi NEMO_Mers Transcriptome (Illumina) SRR2681155Nemertoderma westbladi NEMO_Nemw Transcriptome (Illumina) SRR2682004
Xenoturbellida [1] Xenoturbella bocki XENO_Xboc† Transcriptome (Illumina) SRR8638116Xenoturbella bocki XENO_XbJC Transcriptome (Illumina) SRR2681987
DEUTEROSTOMIAEchinodermata [11] Apostichopus japonicus ECHI_Ajap Transcriptome (Illumina) SRR1185973
Echinaster spinulosus ECHI_Espi Transcriptome (Illumina) SRR1139455
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Eucidaris tribuloides ECHI_Etri Transcriptome (Illumina) SRR1138704Holothuria glaberrima ECHI_Hgla Transcriptome (Illumina) SRR490924Lytechinus variegatus ECHI_Lvar Genome SRX056025Ophiocoma echinata ECHI_Oech Transcriptome (Illumina) SRR1138707Ophioderma longicauda ECHI_Olon Transcriptome (Illumina) SRR1325052Oxycomanthus japonicus ECHI_Ojap Transcriptome (Illumina) SRR1138706Patiria miniata ECHI_Pmin Genome SRX096949Sclerodactyla briareus ECHI_Sbria Transcriptome (Il