Supplemental Figure 2
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Supplemental Figure 2 RPMI8226 cells were incubated with increasing concentrations of the respective HIV PI (0-160 M; Am: amprenavir; At: atazanavir; D: darunavir; I: indinavir; T: tipranavir) and intracellular proteasome activity was assessed using the affinity probe MV151. The bargraphs represent relative changes in proteasome subunit-specific (1/5 vs. 2) fluorescence signals compared to untreated controls. RPMI8226 MV- 151 β- actin [µM] β1(i )/ β5(i ) β2 β2i [kD ] 30 20 45 β2/2 i β5/5i/ 1/1i Change in proteasome activity [%] NLVS 0 10 20 40 0 10 20 40 10 20 40 NLVS 0 10 20 40 0 10 20 40 Am At D I T
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Supplemental Figure 2 - PowerPoint PPT Presentation
Transcript of Supplemental Figure 2
Supplemental Figure 2
RPMI8226 cells were incubated with increasing concentrations of the respective HIV PI (0-160 M; Am: amprenavir; At: atazanavir; D: darunavir; I: indinavir; T: tipranavir) and intracellular proteasome activity was assessed using the affinity probe MV151. The bargraphs represent relative changes in proteasome subunit-specific (1/5 vs. 2) fluorescence signals compared to untreated controls.
RPMI8226 MV-151
β- actin
[µM]
β1(i)/β5(i)
β2β2i
[kD]30
20
45
β2/2iβ5/5i/1/1i
Cha
nge
in p
rote
asom
e ac
tivity
[%
]
NLVS 0 10 20 40 0 10 20 40 10 20 40 NLVS 0 10 20 40 0 10 20 40
Am At D I T