Supp. Table I
description
Transcript of Supp. Table I
MutationRank14-3-3sigma, BDS-2B99BAI1Noxap53AIP1PA26PCNAPiddPTGF-beta, FBS01RGCS100A2
wtR175H 1 - - - - - - - - - - -R248Q 2 - - - - - - - - - - -R273H 3 - - - - - - - - - - -R248W 4 - - - - - - - - - - -R273C 5 - - - - - - - - - - -R282W 6 - - - - - - - - - - -R249S 7 - - - - - - - - - - -G245S 8 - - - - - - - - - - -Y220C 9 - - - - - - - - - - -C176F 10 - - - - - - - - - - -V157F 11 - - - - - - - - - - -M237I 11 - - - - - - - - - - -E285K 13 - - -G245D 14 - - - - - - - - - - -H179R 15 - - - - - - - - - - -Y163C 16 - - - - - - - - - - -R273L 17 - - - - - - - - - - -Y234C 18 - - - - - - - -H179Y 19 - - - - - - - - - - -Y205C 20 - - - - - - - - - - -R248L 21 - - - - - - - - - - -V272M 23 - - - - - - -R158L 24 - - - - - - - - - - -C141Y 26 - - - - - - - - -P151S 27 - - - - - - - - - - -V173L 28 - - - - - - - - - - -R280T 30 - - - - - - - - - - -C238Y 32 - - - - - - - - - - -C242F 32 - - - - - - - - - - -P278S 35 - - - - - - - - - - -P278L 37 - - - - - - - - - - -C135Y 43 - - - - - - - - - - -H193R 45 - - - - - - - - - - -G244S 46 - - - - - - - - - - -R249M 50 - - - - - - - - - - -V173M 51 - - - - - - - - - - -K132R 56 - - - - - - - - - - -G244D 56 - - - - - - - - - - -M246V 61 - - - - - - - - - - -C135F 64 - - - - - - - - - - -K132N 68 - - - - - - - - - - -G279E 69 - - - - - - - - - - -M246I 74 - - - - - - - - - - -C238F 76 - - - - - - - - - - -H179L 81 - - - - - - - - - - -R249W 82 - - - - - - - - - - -P151H 87 - - - - - - - - - - -P177L 87 - - - - - - - - - - -N239S 87 - - - - - - - - - - -R273P 93 - - - - - - - - - - -C277Y 101 - - - - - - - - - - -R175L 106 -F270C 106 - - - - - - - - - - -R267W 119 - - - - - - - - - - -H168R 123 - - - - - - - - - - -D259Y 123 - - - - - - - - - - -H179N 128 - - - - - - - - - - -V143A 147 - - - - - - - - - - -L265P 151 - - - - - - - - - - -A276P 157 - - - - - - - - - - -R280S 157 - - - - - - - - - - -R158P 170 - - - - - - - - - - -P151R 187 - - - - - - - - - - -D281G 187 - - - - - - - - - - -G245R 202 - - - - - - - - - - -M246R 202 - - - - - - - - - - -L257Q 202 - - - - - - - - - - -C176R 219 - - - - - - - - - - -P278H 238 - - - - - - - - - - -L257P 256 - - - - - - - - - - -D281Y 277 - - - - - - - - - - -R283H 277 - - - - - - - - - -Y236D 340 - - - - - - - - - - -M237R 381 - - - - - -T256A 381 - - - - -S127P 417 - - - - - - - - - - -
loss of wt p53 function
Supp. Table I Dearth et al. 5
Mutation N C
wtR175H
R248Q
R273H
R248W
R273C
R282W
R249S
G245S
Y220C
C176F
V157F
M237I
E285K
G245D
H179R
Y163C
R273L
Y234C
H179Y
Y205C
R248L
V272M
R158L
C141Y
P151S
V173L
R280T
C238Y
P278L
C135Y
R249M
V173M
P151H
V143A
P151R
L257Q
Supp. Table II Dearth et al. 6
p53 DBS
MATa MAT
diploid
TRP1CEN
URA3
TRP1CEN
URA3
LEU2CEN
ADH1 p53
MATa
TRP1CEN
URA3
LEU2CEN
ADH1 p53
HIS3CEN
ADH1 p53mut
TRP1CEN
URA3
HIS3CEN
ADH1 p53mut
HIS3CEN
ADH1 p53mut
diploid
p53 mutant wt p53p53 DBS
A
Supp. Fig. 1 Dearth et al. 7
wt (eng)
R248QR273CR282WY220CV157FE285KY234CY205CR158LC141YP151SV272MP278LP278SH193Rwt (nat)
CS
R
cycl
in G
Fas
(AP
O-1
/CD
95)
GA
DD
45G
ML
KIL
LER
/DR
5M
DM
2m
dr1b
p21,
5’ s
itep2
1, 3
’ site
Type
IV C
olla
gena
se
wt (eng)R248QR273CR282WY220CV157FE285KY234CY205CR158LC141YP151SV272MP278LP278SH193Rwt (nat)
B
C
Supp. Fig. 1 Dearth et al. 8
wild
-type
R24
8QR
273C
R28
2WY
220C
V15
7FE
285K
Y23
4CY
205C
wild
-type
R15
8LC
141Y
P15
1SV
272M
P27
8LP
278S
H19
3R
D
Supp. Fig. 1 Dearth et al. 9
wt p53 NC
control NC
R175H NC
R248Q NC
R248W NC
R282W NC
R249S NC
wt p53 NC
C176F NC
M237I NC
E285K NC
H179R NC
G245D NC
H179Y NC
wt p53 NC
Y234C NC
R248L NC
R280T NC
P151S NC
C238Y NC
P278L NC
wt p53 NC
C135Y NC
P151H NC
V143A NC
C257Q NC
P151R NC
p
53
t
op
o II
t
ub
ulin
p
53
t
op
o II
t
ub
ulin
Supp. Fig. 2 Dearth et al. 10
2.51.5 2.0
Relative Activity
0 1.00.5
empty
wild-type
R282W
E285K
Y234C
V272M
R283H
M237R
2.51.5 2.00 1.00.5
empty
wild-type
R282W
E285K
Y234C
V272M
R283H
M237R
A
B
p21, 5’ site37˚C
Noxa37˚C
Supp. Fig. 3 Dearth et al. 11
A
B
2.01.5
Relative Activity
0 1.00.5
empty
wild-type
R282W
E285K
Y234C
V272M
p21, 5’ site30˚C
2.01.5
Relative Activity
0 1.00.5
empty
wild-type
R282W
E285K
Y234C
V272M
Noxa30˚C
0.2 0.6 1.0 1.4 1.61.2
Relative Activity
0 0.80.4
cyclin G
GADD45
GML
KILLER/DR5
MDM2
Pidd
C
30˚Cwild-typeR282W
wild-typeR282W
wild-typeR282W
wild-typeR282W
wild-typeR282W
wild-typeR282W
Supp. Fig. 4 Dearth et al. 12
Supp. Fig. 5
Relative Activity
mut
ant
wild
-typ
e
anti-
p53
R248Q
0.2 1.40.8 1.210
10
10
10
10
10
0
0.60.4
10
10
10
10
10
0
0
10
50
100
250
250
0
10
50
100
250
250
Noxa
PCNA
Dearth et al. 13
Class 1
Supp. Fig. 6
0.2 1.40.8 1.210 0.60.4
Class 3
mut
ant
wild
-typ
e
anti-
p53
anti-
p53
R282W
10
10
10
10
10
0
10
10
10
10
10
0
0
10
50
100
250
250
0
10
50
100
250
250
Noxa
PCNA
Relative Activity
Y234C
0.2 1.40.8 1.210 0.60.4
Relative Activity
0.2 1.40.8 1.210 0.60.4
Class 2
Relative Activity
mut
ant
wild
-typ
e
anti-
p53
R158L
10
10
10
10
10
0
10
10
10
10
10
0
0
10
50
100
250
250
0
10
50
100
250
250
Noxa
PCNA
R273C
Relative Activity an
ti-p5
30.2 0.8 1.210 0.60.4 1.4
Dearth et al. 14
Supp. Fig. 7GML
MDM2
Class 5
mut
ant
wild
-typ
e
10
10
10
10
10
0
10
10
10
10
10
0
0
10
50
100
250
250
0
10
50
100
250
250
Relative Activity
anti-
p53
Y220C
0.2 1.40.8 1.210 0.60.4
Relative Activity
anti-
p53
Y163C
0 0.2 1.40.8 1.210.60.4
Dearth et al. 15