Supercritical Fluid Chromatography - JASCO...

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Supercritical Fluid ChromatographyLow-Cost, Fast, Green Technology

The JasCo advantageJASCO’s modular SFC/SFE platforms have been optimized and refined over the last 20 years to provide reliable, worry-free performance for a wide variety of applications.

• ThepatentedJASCOback-pressure regulatoremploysahigh-speedswitchingvalve toensurethataconstantbackpressureismaintainedatalltimes,regardlessofthegasflowrate.Mostothersystemsusearestrictiondevicewhich isflowdependentandhence isunabletoprovidetheessentialconstantpressureconditions.TheJASCOdesignhastheadditional benefit of very low dead volume (below 10 µL) which prevents fractions from re-mixing.Italsopresentsasignificantreductioninanyprecipitationbuildupintheflowline.

• JASCOoffersawiderangeofdetectorswithhighpressurecells–UV,DiodeArray(real-timecollectionof3-Dspectraandchromatograms),andtheonlyCDdetectoravailableforSFC.

• OuranalyticalSFC/SFEsystemscanbeconvertedeasilytoHPLC.

• JASCO’smodifierdeliverypumpsguaranteestabledeliveryperformanceevenathighflowrates.

• JASCOoffersatrulymodularsystem–fromasimpleextractortoacomplexmulti-instrumentsystem that shares the same basic components.

• JASCOSFCsystemsarecompatiblewithmostMassSpectrometersandofferdirectcontrolin both Excalibur and Analyst software.

• JASCOprovidesprofessional,fastandcourteoussupportonallofourproducts.Wehave33differentservicecentersintheU.S.toensurethehighestlevelofcustomersatisfaction.Ourscheduledandon-sitetrainingclassesareavailableforadvancedapplicationtraining.

• JASCOoffersmulitpleconfigurations,pleaseaskyourlocalsalesrep.

Low-Cost, Fast, Green Technology over the years, JasCo has responded to the growing emphasis on

reducing chemical waste by offering an alternative to traditional

HPLC with a full line of “green” sFC/sFE products. The reduction in the

use of organic solvents has cost, health, and safety benefits as well as

faster, cleaner sample recovery during experimental procedures. These

advantages are a result of supercritical fluids liquid-like densities offering

higher solubility and increased column loading. They have low viscosity

and are highly diffuse enabling faster separation and extraction.

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Contents

2 Features and Benefits

3 SFC Components

3-4 Carbon Dioxide Pumps

5-6 Modifier Delivery Pumps

7 Autosampler

8-9 Column Ovens

9-10 Detectors

11 Back Pressure Regulators

12 Temperature Control Units

13 Valves

14 Vessels

15 Analytical Systems

16-18 Extraction Systems

17-18 Fully Automated Residual Pesticide Extraction Systems

19-20 Preparative SFC

21 Software

22 Service and Support

23 Training Seminars

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Supercritical Fluid Components

supercritical Carbon Dioxide Pumps PU-2080-Co2/PU-2086-Co2/PU-2088-Co2

Supercriticalfluidsystemsrequireapumpfortransferringliquidcarbondioxide.JASCOoffersthePU-2080-CO2 foranalysisapplications, thePU-2086-CO2 forapplications ranging fromanalysistopreparativeisolation,andthePU-2088-CO2forhigh-volume,preparativeisolation.

PU-2080-CO2 Supercritical Carbon Dioxide Pump (Analytical Range)Thiscarbondioxidetransferpumpisforanalysisapplications.ItemploysSlowSuction-QuickDelivery(SSQD),recognizedfor lowpulsationas itspumpingmechanism,therebyenablingstable transfer. It enables transfer at a stablecompositional ratiowhenusing it alongwithamodifierpump,therebyachievingseparationandextractionwithhighreproducibility.ThePU-2080-CO2alsomakesitpossibletobuildanextremelycompactanalysissystemwithoutrequiringanyexternalcoolingequipment.

PU-2086-CO2 Supercritical Carbon Dioxide Pump (Semi-Preparative)Thisdual-headsupercriticalcarbondioxidepumpisforapplicationsrangingfromanalysistopreparativeisolation.Itemploysaspeciallyshapedcamtoachievestablepumpingperformanceup toahighflowrate.Asinglesystemcanbeused toanalyzebatchesandperformpurityinspections.ItalsoconservesspacebycombiningthePU-2086withacompactcoolinghead.

PU-2080-CO2 PU-2086-CO2

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Flow rate (mL/min) 0.2 0.5 7 15 120PU-2080-CO2

PU-2086-CO2

PU-2088-CO2

Delivery capacity at normal flow rate range

Model name PU-2080-CO2 PU-2086-CO2 PU-2088-CO2

Typeofpump SSQDmethod(SlowSuction-QuickDelivery)

Flowratesetting 0.001~10 mL/min 0.001~20 mL/min 0.1~150.0 mL/min

Flowraterange 0.2~7 mL/min 0.5~15 mL/min 5.0~120.0 mL/min

Pressurerange 0~30MPa 0~30MPa(lessthan15mL/min)0~25MPa(morethan15mL/min)

0~30MPa

Max. pressure 0~35MPa 0~35MPa 0~50MPa

Timeprogramming Flowrate,Upperpressurelimit,Lowerpressurelimit,solventswitvhing

Materials All materilas in contact with solvent are sapphire, ceramic, stainless steel, fluorocarbonpolymer,PEEK

Coolingmethod Peltierbuilt-incoolingjacket(circulationbathisrequired.)

Dimension 225(W)x430(D)x315(H)mmApprox.22kg

150(W)x575(D)x150(H)mmApprox.11kg

350(W)x700(D)x260(H)mmApprox.45kg

Powerinputvoltage AC100~240V,50/60Hz AC100~240V,50/60Hz AC100~120V,50/60Hz

Powerconsumption

500VA 185VA 600VA

PU-2088-CO2 Supercritical carbon dioxide pump (Preparative)This dual-head supercritical carbon dioxide pump is specifically designed for preparativeisolation.Dueto itsability to transfercarbondioxideatastable rateof10to120 mL/min,itcanprocesslargevolumesofsamplesuptoabout30mmofcolumninternaldiameter forpreparative SFC applications and up to 1L of vessel volume for extraction applications.


PU-2088-CO2

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Modifier Delivery PumpsPU-2085/PU-2080/PU-2089/PU-2086/PU-2088Supercriticalfluidsystemsthatusecarbondioxideaddamodifiersolventwhenboostingthedegreeofextractionfreedom.Achievingastabilizedcompositionalratio(0.1to50%)iscrucialtoperformingseparationanalysis, requiringapumpthatperformsstablefluidtransfer.ThePU-2080,PU-2085,PU-2086,PU-2088,andPU-2089transferpumpsrenownedfortheuseinHPLC,aretheoptimalchoiceforpumpingmodifiersolvent.

PU-2085/2080 Semi-micro/Analytical HPLC pumpThese pumps are capable of low-pulse pumping due to optimizations of the pump head,checkvalve,plunger,andotherparts,therebydeliveringastablebaseline.JASCO’sproprietarySSQDpumpsdeliverco-solventatastableratewithlowpulsation.ThePU-2085isparticularlyefficientatpumpinginthelowflowrange.ThePU-2085andPU-2080areoptimalforpumping0.1 to 3 mL/min and 0.3 to 7 mL/min, respectively.

PU-2089 4-solvent low pressure gradient pump

ThePU-2089isanall-in-onequaternarypumpthatdeliversallthefunctionalityofthePU-2080HPLCpump, theLG-2080-04four-solvent low-pressuregradientunit,andtheDG-2080-54four-solventdegasser.ThePU-2089canswitchbetweenfourdifferentmodifiersolvents.

PU-2085/2080 PU-2089

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Model name PU-2085 PU-2080 PU-2089 PU-2086 PU-2088

Typeofpump SSQDmethod(SlowSuction-QuickDelivery)

Flowratesetting 0.001~4 mL/min 0.001~10 mL/min 0.001~10 mL/min 0.001~20 mL/min 0.1~70 mL/min

Flowraterange 0.1~3 mL/min 0.3~7 mL/min 0.3~7 mL/min 1~15 mL/min 5~60mL/min

Pressurerange 0~30MPa 0~30MPa 0~30MPa 0~30MPa(lessthan15 mL/min)0~25MPa(morethan 15 mL/min)

0~30MPa

Max. pressure 0~50MPa 0~50MPa 0~50MPa 0~50MPa 0~50MPa

Timeprogramming Flowrate,Upperpressurelimit,Lowerpressurelimit,solventswitching

Materials Allmaterialsincontactwithsolventaresapphire,ceramic,stainlesssteel,fluorocarbonpolymer,PEEK

Dimension 150(W)x470(D)x150(H)mm

150(W)x470(D)x150(H)mm

150(W)x470(D)x225(H)mm

150(W)x470(D)x150(H)mm

350(W)x470(D)x260(H)mm

Weight Approx.10kg Approx.10kg Approx.14.3kg Approx.10kg Approx.34kg

Powerinputvoltage AC100~240V,50/60Hz

AC100~240V,50/60Hz

AC100~240V,50/60Hz

AC100~240V,50/60Hz

AC100~120V,50/60Hz

Powerconsumption 185VA 185VA 185VA 185VA 600VA

Flow rate (mL/min) 0.1 0.5 7 15 60PU-2085PU-2080/2089PU-2086PU-2088

Delivery capacity at normal flow rate range

PU-2086 Preparative pumpThispumpemploysadualplungersystemoptimalforhigh-flowpumping.Ithandlesapplicationsupto20mL/minandcanbeusedforanalyzingpreparativesamplesinjectedathighvolumes.TheunitisthesamesizeasthePU-2080andsavesspaceevenwhencombinedwithahigh-flowpumpingsystem.

PU-2088 High-! owrate pumpThisunitisamodifiersolventpumpdevelopedforpreparativeSFC.Theusualflowraterangeisbetween5and60mL/minandwhenusedwiththePU2088-CO2enablestheprocessingofhigh-volumesamples.


PU-2086 PU-2088

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autosampler for sFCas-2059-sFTheAS-2059-SFautosamplerisusedtomeasuremultiple-specimensamples.

AS-2059-SF Autosampler When using supercritical carbon dioxide as a mobile phase, it replaces the content of thesampleloopwithsupercriticalcarbondioxidewitheachinjection.Continuousintroductionsofsamplesrequirestheaspirationofthenextsampleaftersafelydischargingthecarbondioxideinthesampleloopandfullyreplacingitwithanorganicsolvent.TheAS-2059-SFcansafelydischargecarbondioxide in thesample loopandcontinuously injectsamples.The injectionmethodcanbeselectedaseitherafixedinjectionvolumethatfillstheentiresamplelooporavariable injectionvolume that introduces thesample inaportionof thesample loop.Thevariableinjectionvolumemethodisalsocapableoffullinjectionwithzerosampleloss.

Temperature ControlTheoptionalsample-warmingunit(TC-2059)cancontrolthetemperatureofasamplebetween4ºCand60ºCin1ºCincrements(temperaturecontrolprecision:±1ºC).

Sampleinjectionmethod: Variablesamplevolumeinjectionmethodorfixedvolumeinjectionmethod

Numberofsamples: 120(2.0µLvials,standardrack)54(Largevolume,4.0mLvials)224(300,600µLvials,micro-volumerack)384x2(384-wellsmicroplateX2,microplaterack)

Sampleloop: 5 µL

Injectionvolume: 0.1to100µL(0.1µLincrements)(Standard,volumesover10µLrequireloopreplacement.)1to1000µL(1.0µLincrements)(Optional,largevolumeinjectionkitisrequired.)

Reproducibility: RSD0.5%orless(Whenfixedloop,20µLinjection)

Contamination: 0.01%orless(Whenfixedloop,5µLinjection)

Injectionspervial: 1to99

Analysistimesetting: 0.1to999.9min(0.1minincrements)

Max.usablepressure: 30MPa

Temperaturesettingranges: Optional,4°Cto60°C(Precision:±1°C)

Materials: All materials in contact with solvent are ceramic, stainless steel, fluorocarbonpolymer,PEEK

AS-2059-SF

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Column ovensCo-2060/Co-2065/sFC-sroDensitychangesduetochangesintemperaturehaveamajorimpactontheseparationandextractionprocessonsupercriticalfluidsystems.Achievingstableextractionefficiencyandpeakretentiontimesrequirestheuseofanoventopreciselycontrolextractionvesselsandregulatecolumntemperature.JASCOofferstheCO-2060,which iscapableof temperaturecontrol at or below room temperature and the CO-2065 and SFC-Sro. The SFC-Sro isdesigned for temperature control for cup-shaped, high-pressure vessels and it includes amixingfunction.

CO-2060/2065 Column OvenThe CO-2060/2065 ovens are capable oftemperature control with a precision of ±0.1ºC with excellent reproducibility. TheCO-2060 is for heating and cooling, whiletheCO-2065isforheatingonly.Bothmodelsdetect abnormal temperatures and solvent leaks and include a function for shuttingoff theheat.A manual indicator,preheatingcoil, reaction coil, and other such devices can be installed on both the CO-2060 andthe CO-2065. Furthermore, the CO-2060caneasilyperformnotonlycooling(usedforoptical isomer separation columns), but also temperature increases and decreases by the useofatimeprogram.

SFC-Sro Column OvenThe SFC-Sro oven is capable of heatingfrom room temperatures +20ºC to 150ºC and supports not only ordinary columns and extraction vessels, but also cup-shaped, high-pressure vessels. Inserting a mixerenables the efficient mixing of the vessels’contents,improvingreactionefficiencywithinsupercritical carbon dioxide.

CO-2060

SFC-Sro

CO-2065

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DetectorsUV-2070/2075/MD-2010/2015/CD-2095Evaluatingopticalcharacteristicsunderasupercriticalstate requiresadetectorandahigh-pressureresistantcell.MonitoringsamplesinsupercriticalfluidispossiblebyreplacingtheflowcellsectionofJASCO’sliquidchromatographydetectorwithahigh-pressureresistantcell.

UV-2070/2075 UV/Vis DetectorTheUV-2070/2075UV/Visdetectorcombineshigh performance, multifunctionality, andsuperior operability for most standard detectiontechniquesinchromatography.

Model name CO-2060 CO-2065 SFC-Sro

Temperaturecontrolmethod Heater,Peltier Heater Heater

Temperaturecontrolrange 15ºCbelowambientto80ºC 10ºCaboveambientto80ºC 20ºC above ambient to 150ºC

Accuracy ±0.1ºC(Whentemperatureissetat40ºC.)

±0.1ºC(Whentemperatureissetat40ºC.)

±0.3ºC

Stirrer None None Included(100to1000rpm)

Safetycheck Heaterpowershutoffwhenunusuallyhightemperatureorflammablesolventleaksaredetected.

Heaterpowershutoffwhenunusuallyhightemperatureorflammablesolventleaksaredetected.

Heaterpowershutoff when unusually hightemperatureisdetected.

Column compartment 110(W)×415(H)×83(D)mm 110(W)×415(H)×83(D)mm 240(W)×245(H)×200(D)mm

Dimensions 150(W)×465(H)×470(D)mm 150(W)×465(H)×470(D)mm 310(W)×460(H)×460(D)mm

Weight Approx.19kg Approx.15kg Approx.20kg

Powerrequirements 100~220V,AC,50/60Hz 100~220V,AC,50/60Hz 100~120V,AC,50/60Hz

Powerconsumption Approx.355VA Approx.230VA Approx.650VA

UV-2070/2075

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MD-2010/2015 Diode Array DetectorMultichanneldetectorsarecurrentlyusedinthesamemannerasgeneral-purposedetectors.TheMD-2010and2015deliverhighresolutionandhighsensitivityasaresultofanoptimizedopticalsystemandredesigneddataprocessingcircuits.Theyalsocanbeusedasstand-alonedetectors, even without a data station. These detectors are equipped with a four-channelanalogoutputterminal.Bothtimeandwavelengthaccumulationcanbesetandsamplescanbe measured in the optimal state for the desired sensitivity and resolution.

CD-2095 Circular Dichroism Chiral DetectorThis detector measures optical isomers with circular dichromatic absorption between 220and 420 nm with high sensitivity and excellent selectivity. The CD-2095 is generally 10 to100timesmoresensitivethananopticalrotationdetector.ItcanmeasurebothCDandUVchromatogramsaswellasg-factor(CD/UV)chromatograms.Sinceg-factorinparticularhasaproportionalrelationshipwiththecompositionalratioofopticalisomersamples,theCD-2095can perform compositional measurements and high purity fractionation for non-separatedpeaks. In addition, spectral measurements for CD and UV are possible by stopped-flowspectrometry.

SFC High Pressure Flow Cells

P/N Detector Description

6824-H105A UVDetector High pressure flow cell unit, L=5mm, 4uL gasket: PEEK,window:quartz

6824-H118A UVDetector Highpressurepreparativecellunit,L=1mm

6831-H113A Multi-Channel Highpressureflowcellunit,L=5mmgasket:Pb,window:Detector quartz

6831-H114A Multi-Channel Highpressurepreparativecellunit,L=1mmgasket:PEEK,Detector window:quartz

6838-H106A CDDetector Highpressureflowcellunit,L=20mm

6838-H107A CDDetector Highpressurepreparativecellunit,L=1mmgasket:PEEK,window:sapphire

MD-2010/2015 CD-2095

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Back Pressure RegulatorsBP-2080/BP-2080-MChanges inpressurecangreatly vary thecharacteristicsofSFC.Achievingextractionandchromatogramswithhighreproducibilityonasupercriticalfluidsystemrequiresadevicethatcanperformpressurecontrolinastablemanner.JASCOofferstheBP-2080,whichiscapableof automatic pressure control, and the BP-2080-M, which is capable of manual pressurecontrol.

BP-2080/2080-M Back Pressure RegulatorThe BP-2080 and BP-2080-M employa patented flow-switching valve (FSV)mechanism that enables stable system pressure control even at the lowest volume limitpossible.TheFSVmechanismregulatespressurebycontrollingthechannelopeningwhile a needle vibrates at a high rate ofspeed. This prevents blockages due tohigh-viscosityelutionsamplesandsolidifiedsamples, which can be a problem with static pressure regulators. This enables stablesystem pressure, even during long-termcontinuous operation. Since the BP-2080hasatimeprogramfeaturethatcancontrolpressure and temperature according to thepassageoftime,itmakesiteasytoautomateanalysiswork.TheBP-2080Moffersmanualcontrol for the volume passing through theopeningintheFSVmechanism.

SFC Vch-Bp 6-Way Valve Changer- Fraction CollectorThe SFC-Vch-Bp is an option for the BP-2080 that can be attached to the pressurecontrolsectionof theBP-2080/BP-2080-M.It features a one-channel switching valveandonehigh-pressure1-in-6-outvalve.Thisoption automatically separates the fluideluted from the BP-2080 into test tubes orsample bins. The channel switching valveis used for selecting either collection ordischarge, while the high-pressure 1-in-6-out valve connects downstream from the channelswitchingvalvetofunctionas6-waybranchingvalve.

BP-2080 BP-2080-M

SFCVch-Bp

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Temperature Control UnitsHC-2068-01/HE-2068-01-04/CH-201/CCa-1111 Achieving extraction and chromatograms with high reproducibility on a supercritical fluidsystemrequiresstabletemperaturecontrol.

HC-2068-01/HE-2068-01-04 Heater ControllerTheHC-2068-01isatemperaturecontrollerforheatingsupercriticalfluid.HE-2068-01to04areheatexchangersthatareusedinconjuctionwiththeHC-2068-01,andarenecessarywhenperformingpreparativechromatographyandhigh-volumeextraction.Theseheatexchangersareconstructedwithstainlesssteelpipingandareusedtocontinuouslyheatthefluidflowingwithinthepiping.TheHE-2068-01ismainlyusedforheatingfluidbeforepreparativeisolation.TheHC-2068-02isprimarilyusedafterpreparativeisolation,whiletheHC-2068-04isusedtopreventicingattheoutputoftheautomaticpressurecontrolvalvesandtoheatsamples.

TI-2068-01 Temperature IndicatorTheTI-2068-01isacompact,space-savingtemperatureindicator.Multipleunitscanbeusedsimultaneously.

Circulating Thermostatic BathsThese units use a variety of circulating thermostatic baths to cool carbon dioxide for the PU-2086-CO2andPU-2088-CO2 as well as to circulate the heat that controlls the temperature ofthehigh-pressurevessels.

HC-2068-01

T1-2068-01

HE-2068-01 HE-2068-04

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HE-2068-04

SV-500 SV-500-8 Safety valve

ValvesHV-2080-01/HV-2080-03/sFC-Evc-sr/sV-500-8/safety ValveJASCOoffersavarietyofvalvesavailableforsystemupgrades,includingautomaticvalvesforchannelswitchinganddistributing,stopvalvesforcuttingoffchannels,andmechanicalsafetyvalvesforensuringsystemsafety.

HV-2080-01/hV-2080-03/SFC-Evc-Sr Valve UnitTheHV-2080-01isatwo-channelselectionvalve,whiletheHV-2080-03isa1-in-6distributingvalve.Bothmodelsarehigh-pressurevalvesforautomaticallycontrollingchannelswitching.TheSFC-Evc-Sr isahigh-pressurevalveunit that incorporates two1-in-6-out valves. It isparticularlyusefulincombinationwiththeSCF-Sroforswitchingbetweenmultipleextractionvessels.

SV-500/SV-500-8 Stop ValveThesevalvesareusedtomanuallyopenandclosethechannel.Whenusingcarbondioxide,thesevalvescanstopitssupplytothesystemandmakepumpconditioningeasybyattachingonetotheoutputsideofapump.Thevalvesimprovesystemoperabilityandmaintainability.

HV2080-01 HV-2080-03 SFC-Evc-Sr

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Supercritical Fluid Vessels

High Pressure Vessels

SFC-VCT

EV-1 EV-2 EV-3

SFC-VC1

View CellsViewCellsarecapableofextractingsamplesunderasupercriticalstateviatheobservationwindowandmonitoringtheirreactionprocessesinrealtime.JASCOofferstheSFC-VCIwithtwowindowsinthevesselcylinderandtheSFC-VCT,whichoffersthreewindowsthatprovidea view in the vertical direction as well.

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Supercritical Fluid analytical

SFCThephysical characteristicsexhibitedby supercritical fluid includeadiffusioncoefficientofdissolvedmoleculesthatisahundredtimesgreaterthanitisinliquidandaviscositythatisatleastonefiguresmaller.Asupercriticalfluidchromatographysystem,whichemployssucha medium as a mobile phase, can be expected to serve as a separation analysis method that can rapidly perform separation without any drop in separation efficiency, even at fast flowrates,duetoarapidmasstransferinsidethecolumnwhencomparedwithhigh-speedliquidchromatographythatusesliquidasthemobilephase.

In addition, when carbon dioxide is used as the medium, gasification will occur simply bykeeping theseparatedand fractionatedsampleataconstant temperature,making thisoneofthetechniquescapableofhighlyefficientrefiningwithfewpost-processinghassles,suchaseliminationsolventsafterpreparativeisolation.Thisoffersahostofadvantages,includingcostsavingsrelatedtotheexpenseofpurchasingsolventanddiscardingorganicsolvents,andhighthroughputanalysisandpreparativeisolationduetorapidseparationoverashortperiodoftime.Asagradientelutiontechnique,SFCisabletovarythethreeparametersofpressure,temperature,andmodifiersolventvolume.Itcanbeeffectivelyusedwhenseparatingoligomersandconstituentswithdifferingcharacteristics.(Thetemperaturegradientrequirestheuseofacolumn oven that continuously varies temperature over time.)

0.000

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HPLC

SFC

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Column : OD

Pla

te h

eigh

t (m

m)

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analytical supercritical Fluid Chromatography

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Supercritical Fluid supercritical Fluid Extraction (sFE)

Supercritical Fluid Extraction (SFE) SystemsSupercriticalfluidextraction(SFE)isatechniquethataddsasupercriticalfluidofanextractionmediumtoasamplecontainingaconstituenttargetedforextraction,usingthedifferenceinsolubilitycomparedwiththeextractionmediumtocarryouttheextractionprocedure.Usingsupercritical carbon dioxide as an extraction medium has many advantages and is usedin a variety of fields. Extraction by means of supercritical carbon dioxide can be expected to improve efficiency, including shorter extraction times and simplified procedures whencomparedwithextractiontechniquesthatemployorganicsolvents.Inaddition,itofferseasiersolvent elimination and concentration procedures. Since its critical temperature is a low 31ºC or so, it enables extraction at a near-room temperature state or in a carbon dioxide atmosphere devoidofoxygen.Thismakesitatechniquethatisidealformaterialsthatexhibittemperatureinstability or constituents that are susceptible to oxidation. Furthermore, it has received recent attention as an environmentally friendly extraction technique that does not use hazardousorganicsolvents,ashasbeenadvocatedbythegreenchemistrymovementinrecentyears.

Applications forSFE include theextractionofactiveconstituents, includingvariousflavorsandmedicinal constituents fromnaturalproducts,docosahexaenoicacid (DHA), advancedunsaturated fatty acids and fatty esters such as eicosapentaenoic acid (EPA), fat-solublevitamins, and pharmaceuticals. Other applications include the elimination of unwanted constituents,suchasdecaffeinationanddesolvationwithintablets.Italsocanbeappliedtothepreprocessingofanalysissamples,includingHPLCandGC.

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High pressure extraction vessel

If necessary, add heating coils, detector, and fraction collector.

Supercritical Fluid Extraction System – ConfigurationThe figure below shows the basic configuration and flow path of a supercritical carbon dioxideextraction system. In this system, a supercritical carbon dioxide pump, a modifier solventtransfer pump, an extraction vessel, a thermostatic bath, and an automatic back-pressureregulation valve are connected to the downstream end. The extraction vessel can either be acolumn or cupped vessel, depending on the state of the extraction sample. There is a widevariety of volume types. Extraction parameters include pressure, temperature, and co-solvent/volume. These parameters can be changed to set the optimal conditions.

Fully automated Residual Pesticide Extraction systems

Residual Pesticide Extraction System 3 (10mL)

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Supercritical Fluid supercritical Fluid Extraction (sFE)

Residual Pesticide Extraction System 1 (50 mL)Attention is being focused on extraction techniques using supercritical carbon dioxide fortrace pesticides left behind on food products and in soil. Residual pesticide extraction systems that use supercritical carbon dioxide are a topic of interest at present. JASCO has a lineup of residualpesticideextractionsystemsequippedwiththesecharacteristics.

Features

• Simpleoperation

• Quickextraction-about 30 to 60 minutes per sample, which is one-third to one-forth the processing time of conventional techniques

• Eliminatestracepesticides-the extract is collected in a trap column

• Minimalextractcleanup-also roughly separates impurities in trap column

• Blockagesandcontaminationareminimized-the trap column and piping is washed each time

• Lowrunningcosts-carbon dioxide is highly pure and inexpensive

• Thesystemissafeandenvironmentallyfriendlybecausecarbondioxideisnon-toxicandnonflammable.

Flexibility

• Capacityofextractionvessels:10mLor50mL.The10mLvesselisidealifoperabilityisthefocus.The50mLvesselisabetterselectionifthefocusisonsampleprocessingvolume or analysis sensitivity.

• The50mLvesselofferssimpleone-touchattachmentandremoval.

• Onetosixvesselscanbeprocessedatatime.JASCOoffersthesinglevesseltypeorthesix-vesseltypedependingonthefrequencyofsamplesbeingmeasured.

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Column: CHIRALPAK IA(10mmIDx250mmL)CO2: 30ml/minModifier: CH3OH 4ml/min

Pressure: 20MPaWavelength: 240nmSample: Warfarin 15wt%, 800ml

UV CD

G-factorO

OH

CHCH2 COCOH3

O

Recovery 90%Purity 92%

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Column: CHIRALPAK AD-H

CO2: 15ml/minModifire: CH3OH 1.5ml/min

Pessure: 20MPaWavelength: 310nm

UV CD

G-factor

Theabovephotoshowsapreparativesupercriticalfluidchromatographysystemthatscalesuptheanalysislevel.ThefiguretotheaboveleftisanexampleofusinganI.D.10mmdiameterx250mmopticalseparationcolumnonthissystem,injecting250mgofwarfarinsampleeachtime,usingacirculardichroic(CD)detectorfordetection,outputtingUVandCDatthesametimeasg-factor,andmonitoringthepositionofthegoodstateofopticalpuritywhileisolatingwithhighefficiency.Thefiguretotheaboverightshowsthechromatogramsobtainedthroughcontinuous measurement.

Supercritical Fluid Preparative supercritical Fluid Chromatography

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Supercritical Fluid Preparative supercritical Fluid Chromatography

TheSF-Box-01 and SF-Box-02 areusedforperformingprocesscontrolforotherunits.TheseunitsarerequiredwhenusingSF-NAV.SF-Box-01iscapableofinputtinganalogsignalsandcancarryoutfractiontriggersatdetectorlevelsandslopesinpreparativeSFC.SF-Box-02isthesameasSF-Box-01withouttheanalogsignalinputmodule.ItisusedmainlyforcontrollingSFE systems.

FC-2088-08 Fraction Collector for Preparative SFCTheSF-2088-08consistsofawarmingheaterplacedimmediatelyaftertheautomaticback-pressurevalve,avalveforswitchingbetweencollectionanddisposal,afractioncontrolvalve,agas-liquidseparatorandacollectionvial.Thesettingofvalvesandtemperatureisallperformedbyspeciallycontrolledsoftwareforsupercriticalfluid(SF-NAV).WithpreparativeSFC,alldevicesareinstalledinasinglebox,andanexhaustfanmaintainsdecompression.Thissuppressestheoutflowofcarbondioxideandmodifiersolventinthelab,therebyenablingpreparativeisolationin a clean environment.

ThePD-2000isadistributorforpowerandsignalcontrolandisrequiredwhenusingSF-NAV.ThefrontpanelfeaturesalargeACswitchthatcanturnonoroffsystempower.Itpreventsmalfunctionsbyallowingtheusertoturnsystempoweron/offwithasingleswitchoperationwhichisespeciallyusefulonsystemscomprisedofalargenumberofunits.Italsoplaysacrucialroleinsafesystemoperationbecauseitcandetectsystempowerleakagesandovercurrent.

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Supercritical Fluid software

EZChrom Elite™ Chromatography Data System for Analytical and Semi-Preparative SystemsComprehensive Data analysis

EZChrom Elite is a comprehensive software package that can flexibly handle all types ofchromatography data requirements and demands. These capabilities range from single-channeltodiode-arrayandspectraldatacollectionmodes.IntegratedGPC/SECandsystemsuitability functions are also available. Chromatographers can use EZChrom Elite with the confidencethattheyareusingapowerfulsoftwarepackagethatwillmeetalloftheircurrentandfuturelaboratorydataprocessingneeds.

• GLP/GMPfeatures

• Sensiblephotodiodearraydatahandling

• FullyintegratedtocontrolJASCO’s SFC/SFE,X-LC®andLC-2000Plusseries HPLCsystems

SF-Nav for Preparative SFCSF-NAVisaspeciallydesignedsoftwarepackageforthecontrolandprocessmanagementofSFEsystemsandpreparativeSFCsystems.Inadditiontopumps,autosamplers,anddetectors,itcanalsocontrolautomaticback-pressurevalvesandfractionation.Itcanmonitorandsaveinformation such as temperature, pressure, and CO2flowwithinthesystemduringpreparativeisolation and the extraction process, making it useful when investigating fractionation andextractionconditions. Itcanalsosetfractionationandextractionconditions in linewiththeslopeandthresholdvaluesofsignalsobtainedfromdetectorsandotherdevices.Itenablesfullprocessautomationforpreparativeisolationandextraction.Theprogramcanlisttheoperationstateofunits,showiconsthatinvokeprocessesbysample,anddisplaysamonitorscreenforchromatograms. Itcanalsobatch-saveparameters includingchromatograms,temperature,pressure, and CO2flowandexporttheminASCIIformat.

The operational state of the equipment modules and any abnormalities can be seen on the display. In addition, the display shows the temperature and pressure necessary for process management on a supercritical fluid system, including analog signals from detectors and other devices. The remaining volume in the gas cylinders is displayed based on the volume of CO2 consumed, as calculated according to flow settings.

EZChrom Elite™isatrademarkofAgilentTechnologies,Inc.5301StevensCreekBlvd.,SantaClaraCA95051

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Service and Support Committed to service Excellence

JasCo offers world class service and support. once an instrument is purchased, JasCo’s commitment to you is just beginning. We offer a multitude of services and programs that will keep your equipment operating at peak performance.

Choose the plan that is right for you. We offer four plans to make sure we have one that fits both your budget and requirements.

Advantage SupportTheAdvantageSupportplanofferstheultimatecoverageandincludes:

• Allparts,laborandtravelcosts

• TwoPreventativeMaintenance(PM)callsperyear

• OneIQ/OQservice

• OnefreecustomertrainingclassatJasco,Inc.

• Unlimitedphonesupportfortechnicalandapplicationissues

• 20%discountonconsumableparts

Full SupportTheFullSupportplanoffersexcellentcoverageataveryreasonablecosttoensureyourinstrumentisalwaysinoptimimrunningcondition.Thisplanincludes:

• Allparts,laborandtravelcosts,excludingconsumables

• CustomizedoneortwoPreventativeMaintenance(PM)callsperyear

• DiscountedIQ/OQservice(performedatthesametimeasPM)

• OnefreecustomertrainingclassatJasco,Inc.



Preventative Maintenance Support• OnePreventativeMaintenancecallperyear



JASCOoffersover30servicecentersstrategicallylocatedaroundthebusiestcustomer-based areas in North America to better serve our customers.

JASCO guarantees parts availability and support for 10 yearsafter purchase date of any SFC system or components.

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Application Training SeminarsQuality Training from Jasco, Inc.

Increasingproductivity inthelabrequiresreliable instruments,qualitysupportandastrongworkingknowledgeoftheinstrumentation.JASCOtrainingseminarsarespecificallydesignedtoincreaseyourknowledgeofJASCOproductsandrelatedapplications,aswellasinstrumentmaintenanceandtroubleshootingprocedures.

Weofferbothon-sitetrainingandregularlyscheduledtrainingseminarsatourEaston,Marylandapplications lab. For more information regarding pricing, times and course schedules, orregardingon-sitetrainingatyourfacilitypleasecontactusat800-333-5272.

Our seminars include: Circular Dichroism J-Series, FT-IR Spectroscopy, Fluorescence, UV-Vis/NIR,Polarimetry,HPLC,X-LC®,andSupercriticalFluidChromatography.

Thehands-onaspectofJASCO’sprograms,combinedwithsmallclasssizes,ensurethatyoureceivetheoptimumlearningexperience.

JASCO INTERNATIONAL CO., LTD.4-21,Sennin-cho2-chome,Hachioji,Tokyo193-0835,JapanTel:+81-42-666-1322Fax:+81-42-665-6512www.jascoint.co.jpAustralia,China,HongKong,India,Indonesia,Iran,Korea,Malaysia,NewZealand,Pakistan,Philippines,Russia,Singapore,SouthAfrica,Taiwan,Thailand

JASCO INCORPORATED28600Mary’sCourt,Easton,MD21601,U.S.ATel:+1-800-333-5272Tel:+1-410-822-1220Fax:+1-410-822-7526www.jascoinc.comCanada,CostaRica,Mexico,PuertoRico,Argentina,Brazil,Chile,Colombia,Paraguay,Peru,Uruguay

JASCO EUROPE s.r.l.ViaConfalonieri25,23894Cremella(Lc),ItalyTel:+39-039-956439Fax:+39-039-958642www.jasco-europe.comJASCODeutschlandwww.jasco.de,JASCOUKwww.jasco.co.uk,JASCOFrancewww.jascofrance.fr, JASCO Benelux www.jasco.nl, JASCO Spain www.jasco-spain.com,JASCO Scandinavia www.jascoscandinavia.se, Austria, Finland, Greece, Hungary, Poland,Portugal, Romania, Switzerland, Algeria, Cyprus, Egypt, Israel, Jordan, Kuwait, Lebanon,Morocco,SaudiArabia,Syria,Tunisia,Turkey,U.A.E.

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