Sunday, February 17, 2019

PROGRAM

FIFTEENTH INTERNATIONAL SYMPOSIUM ON RECENT ADVANCES IN ENVIRONMENTAL HEALTH RESEARCH

Sunday, February 17, 2019

12:30 – 4:30 PM Pre-Symposium Workshop on the NIH-National Library of Medicine Web Resources for

Environmental Health Research Facilitated by

Oak Ridge Institute for Science & Education

JSU JAP Science

Building Room 118

5:00 PM Registration Meet & Greet

Marriott Hotel Downtown

Jackson, MS

Monday, February 18, 2019 Marriot Hotel, Jackson, MS

7:30AM – 8:45 AM Registration and Continental Breakfast Mezzanine

8:45AM – 9:30 AM WELCOME & OPENING REMARKS (Dr. Paul Tchounwou – Symposium Chair) Windsor III

Dr. William B. Bynum Jr.

President

Jackson State University, Jackson, MS, USA

Honorable Phil Bryant

Governor

State of Mississippi, USA

Honorable Chokwe Antar Lumumba

Mayor

City of Jackson, Mississippi, USA

Dr. Lynda Brown-Wright

Provost and Vice President of Academic Affairs


Dr. Wilbur Walters, Jr.

Interim Dean, College of Science, Engineering & Technology


PROGRAM


9:30 – 10:50 AM PLENARY SESSION I-A: ENVIRONMENTAL TOXICOLOGY & HEALTH RISK ASSESSMENT

Windsor III

Chairpersons: Dr. Jimmy Efird1 and Dr. Chindo Hicks2

1Professor, Center for Clinical Epidemiology and Biostatistics,

University of Newcastle, 1 Kookaburra Circuit, New Lambton, Australia 2Professor, Department of Genetics, Louisiana State University Health

Sciences Center, 533 Bolivar Street, New Orleans, LA 70012, USA

9:30 AM Mechanisms of Metal-Induced Lung Cancer: Mechanisms of

Particulate Cr(VI)-Induced Centromere Amplification from One

Environmental Health Perspective

Dr. John Pierce Wise, Sr. Wise Laboratory of Environmental and Genetic Toxicology, Department

of Pharmacology and Toxicology, University of Louisville, 505 S.

Prescott St., Louisville, Kentucky, USA

9:50 AM Molecular Mechanism of Nicotine-Induced Carcinogenesis in Kidney

Epithelial Cells

Dr. Kamaleshwar P. Singh

Department of Environmental Toxicology, The Institute of Environmental

and Human Health (TIEHH), Texas Tech University, Lubbock, TX

79409, USA

10:10 AM Licochalcone A Prevents Neuroblastoma Cells from Rotenone-

Induced Apoptosis via the PI3K/Akt/GSK-3β/CREB Signaling

Pathway

Dr. Chia-Wen Tsai Terry Fox Cancer Research Laboratory, Translational Medicine

Research Center, China Medical University Hospital, Taichung, Taiwan,

R.O.C.

10:30 AM Beneficial Effects of Estrogen in Benzo(a)Pyrene [B(a)P] – Induced

Colon Carcinogenesis

Dr. Aramandla Ramesh

Department of Biochemistry & Cancer Biology, Meharry Medical

College, Nashville, TN 37208, USA

10:50 – 11:00 AM Mezzanine

PROGRAM


11:00 AM –

12:20 PM PLENARY SESSION I-B: ENVIRONMENTAL TOXICOLOGY AND HEALTH RISK ASSESSMENT

Windsor III

Chairpersons: Dr. Joseph Landolph1 and Dr. Danuta Leszczynska2

1Associate Professor, Department of Pharmacology/Pharmaceutical

Sciences, School of Pharmacy, USC, Los Angeles, California, USA 2Professor, Department of Civil Engineering, and Industrial Systems and

Technology, College of Science, Engineering and Technology, Jackson

State University, Jackson, MS 39217-0168, USA

11:00 AM Cancer Cell Fusion and Breast Cancer Metastasis

Dr. Felicite K. Noubissi

Cancer Biology Research Laboratory, Jackson State University, 1400

Lynch Street, Box 18540, Jackson, MS 39217, USA

11:20 AM Late and Persistent Effects of Chronic Cadmium Exposure on Multiple

Organs of Rats and Mice: Role of DNA Hypermethylation

Dr. Lu Cai

Departments of Pediatrics, Radiation Oncology, Pharmacology and

Toxicology, University of Louisville School of Medicine, Louisville, KY,

USA

11:40 AM Potential Endocrine Disrupting Effects of Graphene Oxide

Manomaterial on Japanese Medaka Fish

Dr. Asok Dasmahapatra

RCMI Center for Environmental Health, Jackson State University, 1400

Lynch Street, Box 18750, Jackson, MS 30217, USA

12:00 NOON Trisenox Reduces Expression of E2F1, Cyclin E, and Phosphoryation

of P13K Signaling in ALP Cells

Dr. Sanjay Kumar

NIH-RCMI Center for Environmental Health & Department of Biology,

CSET, Jackson State University, Jackson, MS, USA

12:20 – 2:00 PM

LUNCHEON Windsor I

Introduction of Luncheon Speaker

Dr. Emma Fernandez-Ropellet

Professor Pharmacology & Executive Director and Principal Investigator

Center for Collaborative Research in Health Disparities. UPR Medical

Sciences Campus

“Honorary Biomedical Sciences and Health Information Lecture Series”

Improving Minority Health and Reducing Health Disparities: The Role

Played of the RCMI Community

Dr. Elizabeth O. Ofili Professor of Medicine (Cardiology) and Senior Associate Dean for Clinical

and Translational Research. Morehouse School of Medicine.

PROGRAM


2:00 – 3:20 PM PLENARY SESSION II: NANOSCIENCE, NANOTECHNOLOGY AND NANOTOXICOLOGY

Windsor III

Chairpersons: Dr. Sunali Khanna1 and Dr. M. Nasir Uddin2 1Associate Professor, Department of Oral Medicine &Radiology, Nair

Hospital Dental College, Mumbai, 400008 India 2Associate Professor, Departments of Obstetrics and Gynecology,

Pediatrics and Internal Medicine, Baylor Scott & White Health/Texas

A&M Health Science Center College of Medicine, Temple, Texas, USA

2:00 PM Multifunctional Multicolor Luminescence Nanosystem for Trackng

Breast Cancer Heterogeneity

Dr. Paresh Chandra Ray

Department of Chemistry, Physics and Atmospheric Science, College of

Science, Engineering and Technology, Jackson State University, Jackson,

MS 39217-0168, USA

2:20 PM New Formulations of the Rhenium-Platinum Antitumor System Based

on the Quadruple-Bonding Cluster Dirhetenium (III) Compounds

Dr. Natalia Shtemenko

Department of Chemistry, Dnipro Technical University, 19 Dmytro

Yavornytskiy Avenue, 49000 Dnipro, Ukraine

2:40 PM Investigating Toxicity of Fullerene’s Derivatives: A Comprehensive Computational Approach

Dr. Jerzy Leszczynski Department of Chemistry, Physics and Atmospheric Science, College of Science, Engineering and Technology, Jackson State University, Jackson, MS 39217-0168, USA

3:00 PM The Protective Role of Quercetin on Silver Nanoparticle-Induced

Hepatotoxicity in Rats

Dr. Anita K. Patlolla

Molecular Toxicology Research Laboratory, NIH-RCMI Center for

Environmental Health & Department of Biology, CSET, Jackson State

University, Jackson, MS, USA

3:20PM Nanobody-Based Assays and Biosensors to Detect and Assess

Hazardous Chemicals in the Environment and Food: A Creative,

Innovative and Entrepreneural Approach

Dr. Mohamed-Bassem A. Ashour Molecular and Environmental Toxicology Research Laboratory, Plant

Protection Department, Faculty of Agriculture, Zagazig University,

Zagazig-44511, Egypt

3:40 – 3:50 PM Mezzanine

PROGRAM


3:50 – 5:30 PM PLENARY SESSION III: NATURAL RESOURCES DAMAGE ASSESSMENT AND MANAGEMENT

Windsor III

Chairpersons: Dr. Daniel Sarpong1 and Dr. Mehri Fadavi2 1Endowed Chair, Center for Minority Health & Health Disparities for

Research & Education., New Orleans, LA 70125, USA

2Professor and Chair, Department of Chemistry, Physics and

Atmospheric Science, Jackson State University, Jackson, MS, USA

3:50 PM Geospatial Technology Generating Environmental Health

Information for Clinical Practice

Dr. Fazlay S. Faruque

Department of Preventive Medicine and GIS & Remote Sensing

Laboratory, Office of Research. University of Mississippi Medical

Center, 2500 North State Street, Jackson, MS 39216, USA

4:15 PM A New Perspective on Soil Lead and Chiledren’s Blood Lead:

Unflooded Versus Flooded Communities of Metropolitan New

Orleans

Dr. Howard W. Mielke

Department of Pharmacology, Tulane University School of Medicine,

1430 Tulane Avenue-SL8683, New Orleans, Louisiana 70112, USA

4:40 PM Metals Extraction in Water Unsing Filters Packed with Different

Sizes of Mexican Natural Zeolite

Dr. Héctor Rubio Arias

Faculty of Zootechnic and Ecology, Autonomous University of

Chihuahua, Escorza No. 900, Colonia Centro, Chihuahua, Chihuahua,

Mexico. CP:31000.

5:05 PM Methylmercury Screening in Mercury-Contaminated Soils by Cold

Vapor Generation and ICP-MS

Dr. Zikri Arslan


Science, Engineering and Technology, Jackson State University,

Jackson, MS 39217-0168, USA

5:30 PM – 7:00 PM POSTER SESSION A For

STUDENTS

Windsor II

7:30PM – 9:30 PM RECEPTION BY INVITATION (Entertainment and Heavy Hors d’oeuvres)

Marriott Hotel, Jackson, MS

Windsor I

PROGRAM


Tuesday, February 19, 2019 Marriott Hotel, Jackson, MS

7:30 – 9:00 AM Registration and Continental Breakfast

Mezzanine

9:00– 10:20 AM PLENARY SESSION IV-A: NEW FRONTIERS IN ENVIRONMENTAL HEALTH RESEARCH

Windsor III

Dr. Gloria M. Calaf1 and Dr. Timothy Turner2

1Professor, Institute of Scientific Investigation, University of Tarapacá,

Arica, Chile and Center for Radiological Research, Columbia University

Medical Center, New York, NY, USA 2Professor and Chair, Department of Biology, CSET, Jackson State

University, Jackson, Mississippi, USA

9:00 AM Involvement of Marinobufalgenin on Preclamsia: A Squirrel Monkey

Model

Dr. M. Nasir Uddin

Departments of Obstetrics and Gynecology, Pediatrics and Internal

Medicine, Baylor Scott & White Health/Texas A&M Health Science Center

College of Medicine, Temple, Texas, USA

9:20 AM Differential Regulation of Aquaporin-1 and -5 by Xanthohumol in

Lung Cancer Cells

Dr. Maricica Pacurari

Biology Department, College of Science, Engineering, and Technology,

Jackson State University, Jackson, MS, 39217, USA

9:40 AM Ytterbium Toxicity and Mechanism of Action-A Preliminary Study in

Planaria

Dr. Wayne E. Briner

College of Health, Human Services and Science. Ashford University, 8620

Spectrum Center Blvd., San Diego, California, USA

10:00 AM Quantitative Assay of Targeted MicroRNAs Without PCR

Dr. Yiming Liu



MS 39217-0168, USA

10:20 AM –

10:35 AM

Mezzanine

PROGRAM


10:35 AM –

12:15 PM PLENARY SESSION IV-B: NEW FRONTIERS IN ENVIRONMENTAL HEALTH RESEARCH

Windsor III

Chairpersons: Dr. Vincent C. Bond1 and Dr. Ramzi Kafoury2

Department of Microbiology, Biochemistry, and Immunology, Morehouse

School of Medicine, Atlanta, Georgia 30310, USA 2Associate Professor of Biology, College of Science, Engineering and

Technology, Jackson State University, Jackson, MS, USA

10:35 AM Essential Role of JunD in Prostate Cancer Cell Proliferation

Dr. Shafiq A. Khan Center for Cancer Research and Therapeutic Development, Clark Atlanta

University, 223 James P. Bradley Dr., SW. Atlanta, GA, USA

10:55 AM Serum Biomarkers in Oral Carcinogenesis

Dr. Sunali Khanna

Asian Academy of Oral & Maxillofacial Radiology, Member, National

Academy of Medical Sciences, Nair Hospital Dental College, Municipal

Corporation of Greater Mumbai, 400008, India

11:15 AM Mobile Elements Mediate Genetic Instability Following

Environmental Insult

Dr. Prescott Deininger

Tulane Cancer Center, Tulane University, 1700 Tulane Avenue, New

Orleans, Louisiana 70112, USA

11:35 AM Racial Differences in the Inhibitory Effects of Plubagin on the NF-kB

Pathway and CCL2 Release in Triple Negative Breast Cancer Cells

Dr. Karam F.A. Soliman

Center of Excellence for Cancer Research, Training and Community

Service, College of Pharmacy and Pharmaceutical Sciences,

Florida A & M University, Tallahassee, Florida 32307, USA

12:00 - 2:00 PM

LUNCHEON Windsor I

Introduction of Luncheon Speaker

Dr. Traci Hayes, Director of Communications Division, RCMI

Translational Research Network Data Coordinating Center, Jackson State


“Honorary Biomedical Sciences and Health Information Lecture Series”

Protective Effects of Valproic Acid on 6-Hydroxydopamine-Induced

Neuro-Injury

Dr. Da-Tian Bau

Professor at the Graduate Institute of Biomedical Sciences, and Chairman

of Terry Fox Cancer Research Laboratory, Translational Medicine

Research Center, China Medical University Taichung, Taiwan, R.O.C.

PROGRAM


2:05- 3:45 PM PLENARY SESSION IV-C: NEW FRONTIERS IN ENVIRONMENTAL HEALTH RESEARCH

Windsor III

Chairpersons: Dr. Hector Rubio-Arias1and Dr. Jacqueline Stevens2 1Professor–Researcher, Faculty of Zootechnics, Autonomous University of

Chihuahua, Mexico 2Professor & Director of the MARC Program, Department of Biology,


2:05 PM Signs of Carcinogenicity Induced by Malathion and Estrogen in Rat

Mammary Gland Model

Dr. Gloria M. Calaf

Institute of Scientific Investigations, University of Tarapacá, Arica, Chile

and Center for Radiological Research, Columbia University Medical Center,

New York, NY, USA

2:25 PM The Effects and Mechanisms of Prunella Vulgaris Extracts on Lung

Cancer Cells

Dr. Wen-Shin Chang Terry Fox Cancer Research Laboratory, Translational Medicine Research

Center, China Medical University Hospital, Taichung, Taiwan, R.O.C.

2:45 PM Anticancer Potential of Vernonia amagdalina Delite: A Cell-Based Study

Dr. Clement G. Yedjou Natural Chemotherapeutics Research Laboratory, NIH-RCMI Center for



3:05 PM Secretion Modification Region (SMR) Peptide Blocks Migration and

Invasion in Human Brest Cancer Cells

Dr. Vincent C. Bond

Department of Microbiology, Biochemistry, and Immunology, Morehouse

School of Medicine, Atlanta, Georgia 30310, USA

3:25 PM Bioinformatics Study for the Interplay of Environmental and Genetic

Factors Associated with Cancers

Dr. Hung-Chung Huang Bioinformatics and Biostatistics Core Laboratory, NIH-RCMI Center for



3:45–4:00 PM Mezzanine

PROGRAM


4:00 – 5:40 PM PLENARY SESSION V: EMERGING TOPICS IN

COMPUTATIONAL BIOLOGY AND

ENVIRONMENTAL MODELING

Windsor III

Chairpersons: Dr. Dora N. Mbanya1 and Dr. Farshad Amini2

1Professor & Head, Hematology Laboratory, Faculty of Medicine &

Biomedical Sciences, University of Yaoundé I, Cameroon 2Professor & Chair, Department of Civil Engineering, College of Science,

Engineering & Technology, JSU, Jackson, MS, USA

4:00 PM Environmental Health Research at the University of Puerto Rico

Medical Sciences Campus

Dr. Emma Fernández-Repollet

NIH/NIMHD RCMI Center for Collaborative Research in Health

Disparities, University of Puerto Rico Medical Sciences Campus, PO Box

365067, San Juan, Puerto Rico, USA

4:20 PM

Gulf War Illness: Unifying Hypothesis for a Continuing Health Problem

Dr. Anthony R. Mawson

Department of Epidemiology and Biostatistics, School of Public Health,

Jackson State University, Jackson, MS 39213, USA

4:40 PM Selective Recognition of Biologically Anions with Synthetic Receptors

Dr. Md. Alagmir Hossain Department of Chemistry, Physics and Atmospheric Science, College of


MS 39217-0168, USA

5:00 PM Understanding Urbanization and Sea Level Change Effects on the

Spatial Distribution of Mississippi Coastal Wetlands

Dr. Ranjani W. Kulawardhana

Department of Biology, College of Science, Engineering and Technology,

Jackson State University, 1400 Lynch Street, Jackson, MS, USA

5:20 PM Meteorological Effects on Trace Element Solubility in Mississippi

Coastal Wetlands

Dr. H. Anwar Ahmad

Department of Biology, College of Science, Engineering and Technology,

Jackson State University, 1400 Lynch Street, Jackson, MS, USA

5:30 - 7:00 PM POSTER SESSION B – FACULTY /

SCIENTISTS Windsor II

8:00 -10:00 PM SYMPOSIUM BANQUET Introduction of Banquet Speaker

Dr. Marinelle Payton

Chair and Professor, Department of Epidemiology and

Biostatistics, School of Public Health, Jackson State

University, JSU, Jackson, MS, USA

Windsor I

PROGRAM


“Honorary Biomedical Sciences and Health Information

Lecture Series”

Insoluble, Crystalline Nickel Induces Genotoxicity and

Gene Silencing, Which Disrupts 211 Signal

Transduction Pathways and Leads to Differential

Expression of 144 Genes and Morphological, A.I.,

Neoplastic Transformation of C3H/10T1/2 CL 8 Mouse

Embryo Cells

Dr. Joseph R. Landolph

Cancer Research Laboratory, USC/Norris Comprehensive

Cancer Center, and Depts. of Molecular Microbiology/

Immunology and Pathology, Keck School of Medicine,

University of Southern California, Los Angeles, California,

USA

Wednesday, February 20, 2019 Marriott Hotel, Jackson, MS

7:30 - 8:50 AM Registration and Continental Breakfast Mezzanine

9:00 -10:40 AM PLENARY SESSION VI-A: HEALTH DISPARITIES AND ENVIRONMENTAL SECURITY

Windsor III

Chairpersons: Dr. Emma Fernández-Repollet1 and Dr. Anthony

Mawson2

1Professor and Director, NIH/NIMHD RCMI Center for Collaborative

Research in Health Disparities, University of Puerto Rico Medical Sciences

Campus, PO Box 365067, San Juan, Puerto Rico, USA 2Professor, Department of Epidemiology and Biostatistics, School of Public

Health, Jackson State University, Jackson, MS 39213, USA

9:00 AM Translational Research: Is it Old Science in New Clothing? Dr. Daniel F. Sarpong Center for Minority Health & Health Disparities for Research & Education.,

New Orleans, LA 70125, USA

9:20AM Challenges Screening for Transfusion Transmissible Infections in the

Yaounde University Teaching Hospital – Cameroon

Dr. Dora Mbanya

Faculty of Medicine & Biomedical Sciences, University of Yaoundé I,

Cameroon, University and Hospital Centre, Yaoundé, Cameroon

9:40 AM Triple Negative Breast Cancer: Social and Biological Drivers of Health

Disparities

Dr. Lucio Miele

Department of Genetics, Louisiana State University Health Sciences Center

(LSUHSC), and LSUHSC Stanley S. Scott Cancer Center, 533 Bolivar Street,

New Orleans, LA 70012, USA

PROGRAM


10:00 AM Progression of Metabolic Syndrome along with Depression Symptoms

and High Sensitivity C-Reactive Protein: The Bogalusa Hearth Study

Dr. Azad R. Bhuiyan

Department of Epidemiology and Biostatistics, School of Public Health,

Jackson State University, Jackson, MS 39213, USA

10:20 AM Impact of Saharan Dust Transport Over Atlantic Ocean on Particulate

Matter and Ozone Concentrations in San Juan, Puerto Rico, and Miami,

Florida, USA

Dr. Remata S. Reddy

Department of Chemistry, Physics and Atmospheric Sciences, Jackson State

University, 1400 Lynch Street, Jackson, MS 39217

10:45 AM –

11:00 AM

Mezzanine

11:00 AM-

12:40 PM PLENARY SESSION VI-B: HEALTH DISPARITIES AND ENVIRONMENTAL SECURITY

Windsor III

Chairpersons: Dr. Njwen Anyangwe1 and Dr. Mario Azevedo2

1Sceintist, United States Food and Drug Administration, Washington, D.C. 2Dean, College of Liberal Arts, Jackson State University, Jackson, MS, USA

11:00 AM Deconvolution of the Association between Obesity and Triple Negative

Breast Cancer using Genomics Data

Dr. Chindo Hicks

Department of Genetics, Louisiana State University Health Sciences Center,

533 Bolivar Street, New Orleans, LA 70012, USA

11:20 AM Exponentiated, Inverted, and Reflected: The Amazingly Versatile

Weibull Distribution

Dr. Jimmy T. Efird

Center for Clinical Epidemiology and Biostatistics, University of Newcastle,

1 Kookaburra Circuit, New Lambton, Australia, NSW 2305, and Office of the

Dean (Research), Health Sciences Building, ECU College of Nursing,

Greenville, NC 27858, USA

11:40 AM Possible Contribution of Spermatozoa to Poor Conception Rate Among

BaP-Exposed Women

Dr. Anthony E. Archibong

Department of Physiology, Meharry Medical College, 1005 D.B. Todd Blvd,

Nashville, TN, USA

12:00 NOON Approach Improvement in the Management and Prognosis of Dengue

and Yellow Fever in Brazil from 2016 to 2018

Dr. Marcio R. Barbosa

Institute Center of Teaching and Research (CONEP) of Santa Cruz Hospital

– Sao Paulo-Brazil

PROGRAM


12:20 - 3:00

PM LUNCHEON

(CLOSING REMARKS - RECOGNITIONS)

Churchill

LIST OF

ABSTRACTS

PROGRAM


Honorary Biomedical Sciences and Health Information Lecture Series

IMPROVING MINORITY HEALTH AND REDUCING HEALTH DISPARITIES:

THE ROLE PLAYED OF THE RCMI COMMUNITY

A Distinguished Lecture

By

Dr. Elizabeth Ofili, MD, MPH Principal Investigator, RCMI Translational Research Network

Professor of Medicine and Senior Associate Dean

Clinical and Translational Research

Morehouse School of Medicine

Abstract: The Research Centers in Minority Institutions (RCMI) program was established by the U.S.

Congress to support the development of biomedical research infrastructure at minority-serving

institutions granting doctoral degrees in the health professions or in a health-related science. RCMI

institutions also conduct research on diseases that disproportionately affect racial and ethnic

minorities (i.e., African Americans/Blacks, American Indians and Alaska Natives, Hispanics, Native

Hawaiians and Other Pacific Islanders), those of low socioeconomic status, and rural persons.

Quantitative metrics, including the numbers of doctoral science degrees granted to underrepresented

students; NIH peer-reviewed research funding; peer-reviewed publications; and numbers of racial and

ethnic minorities participating in sponsored research, demonstrate that RCMI grantee institutions have

made substantial progress toward the intent of the congressional legislation, as well as the

NIH/NIMHD-linked goals of addressing workforce diversity and health disparities. With the

collaborative network support by the RCMI Translational Research Network (RTRN), the RCMI

program continues to substantially impact the diversity of the nation’s biomedical research workforce.

RCMI scientists lead research discoveries that are improving the health of minority communities and

advancing knowledge in the science of health disparities. RCMI researchers are using advanced

technologies to support collaborations that address cancer, cardiovascular diseases, HIV/AIDS,

neurologic disorders, child health, environmental health, and many other health problems plaguing

underserved and underrepresented populations at disproportionately high rates. Despite this progress,

nationally, many challenges remain, including persistent disparities in research and career development

awards to minority investigators. Funding agencies can play an important role by incentivizing

collaborations, and incorporating metrics in new and renewing research awards that address

underrepresented populations, workforce diversity and health equity.

PROGRAM



PROTECTIVE EFFECTS OF VALPROIC ACID ON 6-HYDROXY-

DOPAMINE-INDUCED NEURO-INJURY


By

Dr. Da-Tian Bau, PhD

Director/Chair, Terry Fox Cancer Research Laboratory

Translational Medicine Research Center,

Professor, Graduate Institute of Biomedical Sciences,

China Medical University, Taichung, Taiwan, R.O.C.

Abstract: Oxidative stress (OS) plays a pivotal role in the pathogenesis of Parkinson’s disease (PD).

6-Hydroxydopamine (6-OHDA) is a neurotoxin used to induce oxidative cell death of dopaminergic

neurons in experimental models of PD. Valproic acid (VPA), a clinically used mood stabilizer, is a

HDAC inhibitor that has been reported to have neuroprotective properties. In this study, we aim at

investigating the feasibility of VPA as a protector for dopaminergic neurons again damage from 6-

OHDA, and the intracellular mechanisms. The 6-OHDA-induced neurotoxicity to the human

dopaminergic cell line SH-SY5Y will be used as the platform for the protective effects of VPA. The

results showed that pretreatment with VPA can improve the cell viability, and significantly reduced the

reactive oxygen species (ROS) induced by 6-OHDA. It is also revealed elevation of p53

phosphorylation and increase of the Bax/Bcl-2 ratio by 6-OHDA can be significantly reversed by

VPA, as measured by mRNA expression and protein level. Taken together, these findings indicate that

VPA is capable of protecting the SH-SY5Y dopaminergic neuronal cells from 6-OHDA-induced

toxicity via the reduction of ROS production, and subsequent attenuation of p53 phosphorylation and

reduction of the Bax/Bcl-2 ratio. It is very possible that VPA could serve as not only a mood stabilizer

but a potential antidote for PD prevention.

PROGRAM



INSOLUBLE, CRYSTALLINE NIS INDUCES GENOTOXICITY (MUTATIONS/GENE

AM PLIFICATION/DELETIONS) AND GENE SILENCING, WHICH DISRUPTS

211 SIGNAL TRANSDUCTION PATHWAYS, AND LEADS TO DIFFERENTIAL

EXPRESSION OF 144 GENES AND MORPHOLOGICAL, A. I., NEOPLASTIC

TRANSFORMATION OF C3H/10T1/2 CL 8 MOUSE EMBRYO CELLS


By

Dr. Joseph Landolph, PhD Director, Chemical Carcinogenesis & Molecular Oncology Laboratory

USC-Norris Comprehensive Cancer Center, School of Medicine

Associate Professor, Department of Pharmacology/Pharmaceutical Sciences

School of Pharmacy, University of Southern California, Los Angeles, California, USA

Abstract: Nickel (Ni) refinery workers inhaling Ni sulfidic ore dusts/smoking cigarettes contracted nasal/lung

cancers. Inhalation of Ni subsulfide (Ni2S3) or green Ni oxide (NiO) induced respiratory cancer in rats. Ni2S3,

green/black NiOs, and crystalline Ni monosulfide (NiS) were phagocytosed into non-transformed C3H/10T1/2

Cl 8 (10T1/2) mouse embryo cells and induced chromo- some aberrations and morphological/A. I./neoplastic

transformation. mRNA differential display showed 144 genes were differentially expressed between green NiO-

transformed (Tx), NiS-Tx, and methylcholanthrene (MCA)-Tx 10T1/2 cell lines vs. non-Tx 10T1/2 cells. We

showed by next generation sequencing (NGS) that in three NiS-Tx 10T1/2 cell lines, there were 250 -300

mutations/Tx cell line. Nos. of missense mutations were > splice site mutations > frameshift mutations >

nonsense mutations. 35/86 proto-oncogenes were mutated in 3 NiS-Tx cell lines. Proto-oncogenes that were

mutated included the NF1, RBM10, Hoxd11, Med- 12, and IGF1R proto-oncogenes. In addition, there were

2,200 alterations in gene copy numbers in the NiS-Tx-cell line, NiS 3A; 1,899 in the NiS-Tx cell line, NiS 3B;

and 1,220 in the NiS-Tx cell line, NiS7A, found by unsupervised hierarchical clustering. There were

amplifications of genes on chromosomes 3, 6, 7, 8, 11, 15, 17, and 18, amplified focal points on chromosomes 4

and 18, and amplification of the Mettl5 gene, encoding a methyl-transferase-like protein. There were also

complete deletions of the tumor suppressor (TS) genes CDKN2A and CDKN2B, encoding cell cycle inhibitors

p16 and p14, and deletions of the CDK1 gene, which plays a role in serine/threonine kinase inhibitor activity in

the 3 NiS-Tx 10T1/2 cell lines. In our model, in Ni-Tx/MCA-Tx cell lines, 6 driver genes were over-

expressed/mutated, causing over-expression of 52 further genes. Nine tumor suppressor genes were under-

expressed/not expressed in Ni+2-Tx/MCA-Tx 10T1/2 cell lines, causing under-expression/no expression of

77genes. These gene amplifications, gene mutations, and gene deletions caused over-expression of 52 genes in

NiS-Tx cell lines, and under-expression/no expression of 77 genes in Tx cell lines. These NiS-induced events

disrupted 211 biological pathways, causing differential expression of 144 genes between non-Tx and NiS-Tx

10T1/2 cell lines, and induction of the Tx phenotypes of morphological transformation, anchorage

independence, and tumorigenicity in the NiS-Tx cell lines.

PROGRAM


Sunday, February 17, 2019

Documents

Transcript of Sunday, February 17, 2019