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![Page 1: Structure and Folding of Ribosomal Frameshift-stimulating mRNA Pseudoknots David Giedroc Department of Biochemistry and Biophysics Texas A&M University.](https://reader036.fdocuments.net/reader036/viewer/2022062805/5697c00f1a28abf838cca3ee/html5/thumbnails/1.jpg)
Structure and Folding of RibosomalFrameshift-stimulating mRNA Pseudoknots
David GiedrocDepartment of Biochemistry and Biophysics
Texas A&M University
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The Central Dogma….
DNA
mRNA
Protein
transcription
translation
REGULATION:Transcriptional regulators; RNAi
Metal Homeostasis/Resistance“A bioinorganic twist”
REGULATION:Ribosomal Recoding, e.g.
-1 Ribosomal Frameshifting
Our interests: Molecular Determinants of Biological Regulation
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Ribosomal Recoding•Ribosomal or translational recoding: A programmed alteration in the usual triplet decoding of the mRNA into protein by the elongating ribosome
•Documented to occur in all organisms (bacteria to mammals to plants)
•Translation recoding signals are embedded in the mRNA itself
•Recoding comes in several flavors:
1) Readthrough via stop codon (UAG) supression: fusion protein
2) Bypass or hopping (T4 gene 60)
3) Incorporation of nonstandard amino acids, includingselenocysteine (#21) and L-pyrrolysine (#22) via stop codon redefinition
4) Frameshifting: change in reading frame to create a fusion protein +1 PRF: XXX YYY ZZ XXX YYZ Z (antizyme: polyamine biosyn; E. coli RF2)
-1 PRF: X XXY YYZ XXX YYY Z (animal/plant RNA viruses; E. coli dnaX)
From Baranov et al. (2002) Gene
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Many RNA viruses employ -1 ribosomal frameshifting
P2
protease
RdRP
fs = 5% antiviral target
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0:-1:
Translational Recoding by -1 Frameshifting (e.g., PEMV-1)
Frameshifting efficiencies typically range from 5-30% in RNA viruses
[ [[ [
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RNA Pseudoknot Folding Topology
Example: Phage T2 gene 32 translational operator
Du, Giedroc and Hoffman (1998) Biochemistry
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Giedroc et al. (2000) J Mol Biol
Structural Diversity in RNA Pseudoknots
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L7/L12
Plant et al. (2003) RNA
Giedroc et al. (2000) J Mol Biol
Modeling the FS signal on the bacterial 70S ribosome
(1JGO)
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Overview of Translation
Pseudoknot kinetically enhances partitioning of the elongating ribosome intothe new -1 reading frame during translocation translocation [Namy et al. (2006) Nature][Namy et al. (2006) Nature].
eEF2 (EF-G)-catalyzedtranslocation perturbed by thedownstream pseudoknot
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EM structure of a stalled 80S ribosome-pseudoknot complex
mRNA channel
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Frameshift-stimulators are structurally diverse
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Identify key molecular features that modulate frameshift stimulation,independent of folding
Evaluate the functional importance of these interactions in detail in a suitable mechanistic assay
Some general feature(s) of pseudoknot structure, stability and/orkinetic lability are major determinants for frameshift stimulation
Our approach:
Systematically investigate the structures, stability determinantsof a group of closely related -1 PRF-stimulating pseudoknots
Hypothesis:
Objective:
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Smith and Barker (1999) The Luteoviridae (CABI)
Typical luteovirus particlesSymptoms of infection with pea enation mosaic virus (PEMV-1)
Mosaic yellow pattern on leafs
Enations
Plant Luteoviruses
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Beet Western Yellows Virus Pea Enation Mosaic Virus (RNA-1) Sugarcane Yellow Leaf Virus
BWYV infection of escarole
Proposed 2º structures of BWYV, PEMV-1 and ScYLV P1-P2 pseudoknots
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Su, Egli, Rich et al (1999) Nat Struct Biol
1.6 Å structure of the BWYV pseudoknot
L2
S1
S2
L1
A23
A24
A25
5’
3’
C8
U13 (L3)C22
A21
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Sugarcane Yellow Leaf Virus P1-P2 RNA Pseudoknot as a Structural Target
Slip-site
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NMR Spectroscopy as a Biomolecular Structural Tool
Texas A&M (500, 600 MHz:) The Scripps Research Institute (900 MHz)
Large fixed Bo: Nuclear magnets (protons, etc.) align in the magnetic field, and absorb radiofrequency energy; the decay of this excited state is a strongfunction of structural environment of individual atoms.
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The predicted ScYLV P1-P2 mRNA pseudoknot adopts a well-folded PK conformation
NH2 protons of C27 reside in an unusual environment
H5(C5C4N)H:
Cornish, Hennig, Giedroc (2005) Proc Natl Acad Sci
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Loop L2 adenosine amino protons are resolved and protected from solvent exchange
Cornish, Hennig, Giedroc (2005) Proc Natl Acad Sci
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50±14º
Solution structure of the ScYLV P1-P2 RNA pseudoknot
Cornish, Hennig, Giedroc (2005) Proc Natl Acad Sci
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Solution Structure of the ScYLV P1-P2 RNA Pseudoknot
S1
S2
L2
5’
3’
C25
G9 (L1)
A13
Cornish, Hennig, Giedroc (2005) Proc Natl Acad Sci
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S1
S2
L2
C25
G9 (L1)
A13
C8+
Cornish, Hennig, Giedroc (2005) Proc Natl Acad Sci
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ScYLV Pseudoknot: Five consecutive base triples
*See also HCV IRES (Kieft et al., 2002) *See also A riboswitch (Serganov et al., 2004);G riboswith (Batey et al., 2004) [C•(U-A)]
cis-Watson-Crick/sugar edge base pairing
Hoogsteen base pairing
Cornish, Hennig, Giedroc (2005) Proc Natl Acad Sci
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•Helical over-rotation: 98º•Horiz. displacement: 5.0 Å
•Helical over-rotation: 89º•Horiz. displacement: 5.5 Å
•Helical over-rotation: 103º•Horiz. displacement: 7.6 Å
CURVES analysis of BWYV, PEMV-1 and ScYLV pseudoknot topologies
BWYV
PEMV-1
ScYLV
S1
S1
S1
S2
S2
S2
A-form coaxial helices
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The ≈2.5-fold difference in FS stimulationbetween ScYLV and BWYV pseudoknots
derives entirely with a 3’ C A substitution
in loop L2
-1 Frameshift Stimulation by the ScYLV P1-P2 RNA Pseudoknot
Cornish, Hennig, Giedroc (2005) Proc Natl Acad Sci
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The structures of the C27A and WT ScYLV pseudoknots are essentially identical…
Cornish, Stammler & Giedroc (2006) RNA
…despite easily measurable structural perturbations at the helical junction region
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The C27A ScYLV pseudoknot is destabilized relative to the WT RNA
Cornish, Hennig, Giedroc (2005) Proc Natl Acad Sci
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Helical junction pairwise coupling free energies () of WT and C27A pseudoknots
Cornish & Giedroc (2006) Biochemistry
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The mechanical model for stimulation of -1 PRF during translocation
Prediction: More stable pseudoknots would be more effective frameshift-stimulators,generally consistent with our findings.
However,stabilizing interactions localized in the helical junction region appear far more important.
Hypothesis: Helical junction interactions may function as GATEKEEPERS (kinetic barrier) to ribosome-mediated pseudoknot unwinding.
Ian Brierley, Univ of London
Namy et al. (2006) Nature
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Another perspective on ribosome-mediated unfolding during -1 PRF
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So the moral of the story is…….
Embrace Physical Chemistry!!
NIH Predoctoral Training Programs in Biophysical Chemistry,Chemistry-Biology Interface
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Conclusions
Plant viral -1 frameshift-stimulating mRNA pseudoknots adopt unique triple helical architectures characterized by numerous loop-stem (L1-S2 & L2-S1) base triple (quadruple) interactions.
Despite significant differences in the helical junctions among all three luteoviral pseudoknots, their global folds are remarkably similar.
A major determinant for modulating frameshifting efficiencies by luteoviral pseudoknots is the 3’ nucleotide in loop L2. We propose that the helical junction functions as a kinetic barrier to ribosome-mediated pseudoknot unfolding. Ground- state structure is a poor predictor of frameshift-stimulation.
These variant pseudoknots will be excellent tools with which to mechanistically probe how pseudoknots stimulate frameshifting (laser-based optical tweezers).
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Acknowledgments
Dr. Carla TheimerDr. Paul Nixon
Dr. Peter CornishSuzanne Stammler
Lichun LiSaritha SuramDr. Raza Khan
Dr. Mirko HennigThe Scripps Research Institute
Dr. David W. HoffmanUniversity of Texas at Austin
NIHNSF
Texas Higher Education Coordinating Board
Dr. Peter Cornish