Antibodies for Cancer research fra CST, Nyt NEBkatalog med fine giveaways, L7 hPSC CultureSystem fra Lonza samt nye produkter fra NEB

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Stem Cell Transfection usingNucleofector™ Technology

Transfection of stem cells is typically a very difficulttask using non-viral methods. With theNucleofector™ Technology stem cells can beconsistently transfected at high efficiency forvarious applications, comprising those that requireco-transfection of several substrates:

• Proven for genome editing in ESCs or iPSCsusing ZFN, TALEN, and CRISPR systems• Efficient episomal reprogramming of PBMCs,fibroblasts, CD34+ or other cells into iPSCs• Optimized, ready-to-use transfection protocolsfor primary adult stem cells such as mesenchymalstem cells (MSCs)or neural stem cells (NSCs)

View archived webinar and learn about genomeediting in hard-to-transfect cells

L7™ hiPSC Reprogramming andhPSC Culture System

The L7™ hPSC Culture System is a robust culturesystem that supports every other day feeding ofpluripotent stem cells under defined and xeno-freeconditions. This xeno-free, fully defined systemincludes a medium, matrix, passaging andcryosolution. L7™ is a complete system for themaintenance and expansion of human embryonicstem cells (ESCs) and induced pluripotent stemcells (iPSCs).

• Reliable reprogramming of peripheral bloodmononuclear cells (PBMCs) into iPSCs• Robust culture system that supports every otherday feeding of pluripotent stem cells• Efficient differentiation into all three germ layers• Easy transition to clinical applications -xeno-free, defined

Find out how to easily generate inducedpluripotent stem cells from PBMCs or other cells

using the Nucleofector™ Technology.

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NYHED!NEB® Golden GateAssembly Mix

Did you know that NEB’s restriction enzymes andligases can be used for Golden Gate Assembly, atechnique for the efficient and seamless assemblyof DNA fragments? Our new Golden GateAssembly Mix incorporates digestion with BsaI andligation with T4 DNA Ligase into a single reaction,and can be used to assemble up to ten fragmentsin a single step.

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NYHED!Rapid PCR Cleanup Enzyme Set

NEB Rapid PCR Cleanup Enzyme Set consists oftwo recombinant enzymes, Exonuclease I (Exo I,#M0293) andShrimp Alkaline Phosphatase (rSAP, #M0371). Itallows for rapid and complete enzymaticdegradation of residual PCR primers anddephosphorylation of dNTPs subsequent to PCR.The Rapid PCR Cleanup Enzyme Set enablesdirect downstream applications includingsequencing, genotyping, cloning or SNP analysiswithout the need for additional purification steps.The Rapid PCR Cleanup Enzyme Set is addeddirectly to the PCR product after thermal cyclingand is 100% compatible with commonly used PCRreaction buffers.

Bestil gratis prøve hos BioNordika!

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NYHED!Antibodies for Cancer Research:PD-L1, PD-1, OX40, OX-40L, CTLA-4,CD40L validated for Flow and IHC

• High Sensitivity: detect endogenous levels of target proteinexpression in human tissues or primary human cells• Target Specificity: no cross-reactivity and tested in relevant

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CD40 Ligand (D5J9Y)Rabbit mAb #15094• CD40 ligand is expressed on T cells andactivates signaling cascades such as MAPK andNF-κB when bound to CD40• WB, IP, and IHC-P validated

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PD-L1 (E1L3N®)XP® Rabbit mAb #13684• PD-L1 (B7-H1, CD274) inhibits activated T cellsby binding to its receptor, PD-1, on the surface ofactivated T cells.• IHC, flow, WB, IP and IF validated

Se flere PD-L1 mAb varianter

NEBNext® Ultra™ DNA LibraryPrep Kit for Illumina®

When determining which NEBNext product to

NEBNext® Ultra™ RNA LibraryPrep Kit for Illumina®NEBNext Ultra kits are available for directional(strand-specific) or non-directional library

PBMC models• Monoclonal Antibodies: ensure lot-to-lot consistency• Optimized Protocol: provided for key applications• Custom Formulations available: for labeling and platform specific applications

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PD-1 (D3O4S) RabbitmAb & Conjugates

• PD-1, expressed on T cells,regulates immune tolerance bysuppressing T cell signaling whenbound to PD-L1 or PD-L2• Flow cytometry validated

Se PD-1 mAb varianterne her

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choose, you’ll need todecide whether to use theUltra or Standardworkflows. Ultraworkflows are faster, havefewer components andclean-up steps, and areoptimized for lower inputamounts. For DNA, theUltra workflow library

preparation is complete in 2.5 – 3 hours, with only15 minutes of hands-on time. The Ultra workflow isoptimized for input amounts between 5 ng and 1µg and is also ideal for ChIP DNA. When time is ofthe essence and starting material is precious, Ultrakits ensure optimal yields.

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preparation. These kitsutilize streamlinedworkflows and novelmaster mixes, and havebeen designed forperformance with inputamounts as low as 10 ng(purified mRNA or rRNA –depleted RNA) forDirectional RNA and

non-directional RNA. Standard non-directionalworkflows are enabled by our standard kits, whichsupport input amounts above 50ng.Our novel Small RNA workflow has beenoptimized to minimize adaptor-dimers, whileproducing high-yield, high-diversity libraries.

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SE HER!Bestil det nye NEB 2015/16 katalog hos BioNordikaog få et minutur, en floatie, en nøglering og en blyant fra NEB!

Eller bestil på mail info@bionordika.dk

BioNordika Denmark A/S - Marielundvej 48, 1. - 2730 Herlev - 3956 2000 - info@bionordika.dk - Afmeld

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