InfoCard #: FLOW-GEN-001 Rev. 05 Effective Date: 19 Oct 2020

STEM CELLLABORATORY (STCL)

DOCUMENT NUMBER: FLOW-GEN-001

DOCUMENT TITLE:Routine Maintenance of BD FACSCalibur Flow Cytometers

DOCUMENT NOTES:

Document Information

Revision: 05 Vault: FLOW-General-rel

Status: Release Document Type: FLOW

Date Information

Creation Date: 14 Sep 2020 Release Date: 19 Oct 2020

Effective Date: 19 Oct 2020 Expiration Date:

Control Information

Author: REESE008

Previous Number: FLOW-GEN-001 Rev 04

Owner:

Change

REESE008

Number: STCL-CCR-490

CONFIDENTIAL - Printed by: BJ42 on 19 Oct 2020 01:56:53 pm

InfoCard #: FLOW-GEN-001 Rev. 05 Effective Date: 19 Oct 2020

FLOW-GEN-001ROUTINE MAINTENANCE OF BD FACSCALIBUR

FLOW CYTOMETERS

1 PURPOSE

1.1 This procedure should be followed when performing maintenance or whiletroubleshooting malfunctions on the BD FACSCalibur flow cytometers.

2 INTRODUCTION

2. 1 In order to assure the reliability of the BD FACSCalibur Flow CytometrySystems, daily, monthly, and semi-annual programs of preventive maintenancemust be performed routinely. In addition, guidelines for minor troubleshootingmust be available to the flow cytometry technologists should an instrumentmalfunction, since quite often the malfunction is remedied with simpletroubleshooting techniques. A log book for written documentation of routinechecks or problems is maintained in the flow cytometry section of the lab.

3 SCOPE AND RESPONSIBILITIES

3. 1 This process should be performed as specified on each BD FACSCalibur FlowCytometer. The laboratory director, manager, flow cytometry supervisor andflow cytometry staff are responsible for ensuring the requirements of thisprocedure are successfully met.

4 DEFINITIONS/ACRONYMS

4. 1 DI-Deionized

4. 2 BD-Becton Dickinson

4. 3 PM-Preventive Maintenance

4.4 PN-Part number

4. 5 MLS-Milliliters

5 MATEMALS

5. 1 Deionized Water

5.2 Bleach, Clorox

6 EQUIPMENT

6. 1 FACSCalibur flow cytometer, Becton Dickinson

7 SAFETY

7. 1 See MSDS for Bleach

8 PROCEDURE

8. 1 Daily Maintenance

FLOW-GEN-001 Routine Maintenance ofBD FACSCalibur Flow CytometersSTCL, DUMCDurham, NC Page 1 of 3

CONFIDENTIAL - Printed by: BJ42 on 19 Oct 2020 01:56:53 pm

ICM

§0)

Iu

±

IUy

i

I

§0

0 ^ri Isu0

I

U2c

IIw

I "I

^

"v>

^3+->

^e

,0

a,<u

-0-0

w

'=3c

5 &^

u

-s

IIu ~F&, &.

(N

00 00

-0

Iu

>n ^

Q ^ -5u

i ^°I!

§i1.1133" QWl W

m

00

^r

00

ir>

00

sIu

.c

<0

00

§

iVIu

T3u

I.

'5 s^ -°.ti 3'^li§-1^ s.^ ^5 u

5 &P

II^1g 'u-c ->>

It^ 0

^!I-5li

M

00

M

00

&flc

IID

u

^

-a-0

VS

-0§

II?3u

^

0

c

&fic

^33

I^

u

i§^

g

1u

g

(N(M00

o -o2 su <u

..!=: "?= 2<-»

Q 2^1^ -c

Ul

m

<N00

U 0-!- . t^

g<u

5 -o^ y^-!s s^ g:£ S<u Q.

Ul

^'^u E-e

S "5y i-S u

u

<u^=

i ss<21^INII1" ^1 t>0u '^ <-^c -^ .?1^-iIllQ "S3 -o

^l-CN00

^^ fn -

B 5d

T3§IU

dUl

-(->

IIi ^u o

3 ^s?°:o <u

st4-0

M

s0

(N

^ 3

I^ s'y ^i-2

cdS 2i sp., .tt

.n

r-l00

Q -S

S S £^Mc

s

I8u

G0

s M(U

0

tu

J=

u

^

<0M

00

0

w

s0

r^l00c

c

^ ...c "

s Is -I§'sI?!-211i-t

_0s ^

II§^^ §2 §^^tS -0

uucd0-"'

c5

<uucj

&u^(^

n

^

03

?

(^M

00

ts-^

IIi sII^ 5i ^Isi§'s .^CTS ^

. fa 's'I§-ii;!sIJS Sd !-,

.c u'w &"5 ='0 "

IIu ^

I ^&1

00(N00

§

IU

-

Iu

-0 S

-^^ss-^.

11" cw

u

I!

cd"

'<->

C/l

Mc

ICO

??u

^=uo

J=^->

-&sc

^1§5s ^110 .S

licd ^

iii I

0^(N00

^ sc <s^slii.j's w^ .sI](U CO

!iII§ Q

& "u -S

^ ?.s-a M.S

ill^11

M

00

ig

I

II

VS

s<u>

^

<u

>. &00

I <u

IIII1iI c-u u

rt £"

U £303 M

S .5

M

s

Iu

<u>

1£

m

00

ti3

73u

iscd

2uu

s

I3 5

'ii §§ "^ ulit"? Q fcIS|Q -c 003 ^ %

i§i"L <-; ^

s! s6--F-s i i11 Io 3 gs-s Ilit^1:

CQ

I

r<~i

00

u

I§

m

^

10

s

I

§01

0

9

§

8

il^.9 Q 's^'^l0 U ^^ii

" ^

§

s

§s

I

&

su

ItT3

I

Illi||y -3 i-l

ls^^^0T? "-5 SIll

> ^1 y i^^^

.^|'s"-5 c:

^ §1ID I I

[0

>>cf -S

pf^ s ^1^ ^l^i? ~o -2y jj C

^s^-a .2 "==i-S .S^ G -C'0. rt -^i s ^s I ^£'11E- S -o

M

rr)

00

e

.24->

I

Isl

^0

(^00M

!19i<

li

VSuVI

^u

^=

I

.I1M

-y^

i^

00

I-'M

g^s ^1.&u

~a

g)t30

S -B0 %

1.1^ 's-0 en

11

s^.s

s^ .2 ^^ 3

IIrt VI

^'s0^S 9^5 so

K'iIIlll"3 ^.^i^1s ^11&1IIII

iC3w^Iu

<uA

on

Iv

'^'s

cdw

I

ll<u

^t-oc

-0_u _§g

^ -po _u^

2 -° -c 'c u

1^1 11I 11

u

S s.

M

^.t-00

uu

Ig

5

3

^.

!?0

u-

Icdu&<3u<fc

Qm

s0

(^2^3^'[-L. ^-1

M

0>

r-~§c^

I<u

I2:<

I(^Mm

3xcS3ccdp

^Ul

§

S P-I E

^ ^ 2y 3 3N s iS < 2

0\

3!"-woavtjautw0

aQ

I

I

1.0

-5s

<

0

z

ICCi

iff

s.s.^11i!li0~> cdc ^

. 2 ^'^ %'y -<M 0

.? .sII<L> U

IIM

yenyu

(^

2

Vi0

<u5tl

2su

s0

&^0

i-t.

Isc/?u<fc

Q03<+1'Bs

IC3

s<ue

3Q(^

00

w0

^QJE

us3Q

^-iuF.Vl

u^

IT;3Q

Ea.

coLOcos>

0

Ic^

§01

g

!CD

s-0sc

£

<

zLUQ

i0

InfoCard #: FLOW-GEN-001 Rev. 05 Effective Date: 19 Oct 2020

Signature Manifest

Document Number: FLOW-GEN-001

Title: Routine Maintenance of BD FACSCalibur Flow CytometersEffective Date: 19 Oct 2020

Revision: 05

All dates and times are in Eastern Time.

FLOW-GEN-001 Routine Maintenance of BD FACSCalibur Flow Cytometers

Author

I Name/Signature I Title , Date ! Meaning/Reason

Melissa Reese (REESE008) 29 Sep 2020, 03:43:31 PM Approved

Management

I Name/Signature [Title I Date , Meaning/Reason

^AbTaSt r'pick 29 Sep 2020, 05:25:30 PM Approved

Medical Director

[Nanwrejgna^ure_L[^_^. «-T^_----»--^^«^^. J»^tl_-»-T. _-^Joanne Kurtzberg (KURTZ001) 29 Sep 2020, 06:34:46 PM Approved

Quality

^ me/agnature___^_[. T!tle-. _".. ".... _._-. "..-__!j;late........................... ........ -T. _'Meaning^Res!sonIsabel Storch(IMS19) 30 Sep 2020, 12:42:19 PM Approved

Document Release

L!^^^§?Sj0^j^_-_..-_....... ^... _...._i.Z!!!l.._..,... --_. "..........."-"..-J..5£!S...^-.__-., -._I.JV!eaning/Reason.Sandy Mulligan (MULL1026) 09 Oct 2020, 06:44:14 PM Approved

CONFIDENTIAL - Printed by: BJ42 on 19 Oct 2020 01:56:53 pm