Std 12

Inhalt

Sene Page1. Einzelteile und

Funktionen1. Individual Components

and their Function1 2

2. 3 2. Applications 13Anwendung3. Funktionsprlnzip 3.4 Principle of Operation 14

3. 1 . Umwalzvotumen3..2. Abscheidevotumen

45

3.1 . Sampling Vol.ume3.2.. Separation Vo(umen

1415

4.. Bedj en ungsanJeitung 6 4. Instructions for Operation 16

6 16

66777

1617171718

4.1 . Sterilisation undDesinfektion vcr demGebrauch

4.2. Einlegen desLuftkeimindikators

4.3. Einschaften des Gerates4.4. Zeiteinste.Jlung4.5. Start en des Gerates4 .6. Abschalten des Gerates4.7. Emnahme des

Luftkeimindikators

4.1 . Sterilization andDisinfectionbefore Use

4.2. Inserting theAgar Strip

4.3. Switching theInstrument. ON

4.4. Setting the Sampling Time4.5. Starting the Instrument4.6. Stopping the Instrument4.7. Removing the Agar Strip8

5. Auswertung 8 5. EvaJuation

6. St6rungsmetdungen 9 6. Fau1t Indicators

7. Wartung undInstandhatt.ung

7. Care andMaintenance9 19

91010

192020

7 .1 . Batterieleistung

7.2. Geometrie der LOfterflOgel

7.3. Laufzeit

7 .1 . Battery Power7 .2. Angle of Impeller Blades7.3. Sampling Time

8. Zubehor 8.11 21Accessories

9. Technische Daten 9.11 Technical Data 21

10. 12 10. WarrantyGarantiebedi ng ungen

I. In91vloual \Jomponen'ts ana 'tnelr Function

Open end impeller drumwith impe11er blades

SJot for insertion of theAgar Strips

[ON/OFF] key: majnswitch for switching theInstrument on and forstarting or interrupting themeasurement process

Infrared sensor LED indicator for impelferblade obstruction

LED indicator for standbyand operation LED indicator for Jow

battery voJtageLED indicators forsampling time Connection for mains

adapter[SELECJ] key for selectingthe sampling time Battery compartment

[RESET] key for resettingerror indicators

Cover for batterycompartment

The Standard RCS Air Sampler is" c ,. '6 "4' ' t. . + f 'a conVentofu tns rumen.. Of mea:~sunng the c:oncentration of air~borne microorgcanrsms.

The instrument can be used inthe fOlloWing areas:

food and beverage industries..Possible investigations iniclude:

pharmaceutical and medicaldevice industries

.the generaJ microbiologicalquality of ambjent air

.. cosmetics Industry. the efficiency of SIr treatmet1t

and air Cleaning equipment hospitals.public health offices.the effectiveness of deconta-

mination measures.

jndustr.iaJ hygjene.,

h b dBnlmal ! us an, ry.

The' Slotest RCS Air Sampleroperates on the impaction princi-ple. The function of the air samp-ler is to deposit. airborne microor-ganisms quantitatively onto a cul-ture medium.

RCS Air Sampler exhibits a spe-cial volome characteristic.. It istherefore necessary to differ-entiate between the samplingvolume (= total volume sampled)and the separation volume(= relevant volume for particleseparation)..

3.1. Sampling Volume

The air flow enters the impellerdrum concentrically with a dia-meter of 2a and at velocity Cax. isdeflected by the impeller bladeand fed over the agar strip. Theair flow exists via an annular gapwith width b. The total samplingvolume (v) can be determinedthrough a point -by-point measu-rement of the velocity and angleof flow over the radius r and sub-sequent mathematical evalua-tion. This sampling volume is280 I/min at the instrumentspeed of 4096 rpm. This samp-ling volume is the basic para-meter for calculating the separa-tion volume.

The air under examination isdrawn into the sampler from adistance of at least 40 cm bymeans of an impeller. The air flowenters the impeller drum concen-trically and in a conical form, isset in rotation, and the particlescontained in the air are impactedby centrifugal force onto an agarmedium. The air then leaves thedrum in a spiral form around theoutside of the cone of air enteringthe sampler. After sampling, theagar strip is incubated and thecolonies are counted.

Due to its principl.e of opera-tion and the geometric proper-ties of the impeller system. the

3.2. Separation Volume

The particles in the air flowbetween the impeUer blade andagar'surface are acceJ.erated bycentrifugal force and impactedonto the agar surface. However,only part of the total samplingvolume is re1evant for particleseparation. This separation volu-me can be determined mathema-ticafly, whereby the height of theimpeJJ.er drum is the most impor-tant parameter. This height (Imin)can be calculated for the separa-tion of all part.ic.les contained inthe total sampling volume. Thebasis for this is the resolution of adifferential equation which descri-bes the spiral flight path of theparticles under the influence ofthe air flow velocjty, the directionof flow and the centrifugal forcethat arises.

whereby

ro = angle veloc.ity11 = viscosity of the airdJ;.t = d.iameter of a relevant

particle~ = density of the particlev = sampling volume

For a relevant particle qiame-ter of 4 ~m, this produces aheight (lmin) of 1 4 cm. However,since onfy 2 cm of 1 4 cm areavailable in the impeller drum asthe separating height, separationis not effected from the totalsampling volume but only from 1/,of this.

Thus separation volume forthe instrument is 40 liters perminute based on an averageparticle diameter of 4 I..Jm..

~

The RCS Air Sampler has beendesigned to operate using 4 x1.5 Volt monocells (1.5 Volt aJkaJi-ne 0 cells, type LR20). The useof standard NiCd rechargeablebatteries (4 x 1..2 V) is not recom-mended. as these have insuffi-cient voltage.

the agar surface facing towardsthe impeller blades. Continue in-serting so that the impeller drumis completely enclosed and thegrip tab of the agar strip protru-des byapprox. 2 cm..

4.1. Sterilization andDisinfection before Use

The impeller may be carefullyremoved and the impeller drumunscrewed from the instrumentfor autoclaving at 1 21°C for 20minutes. The unit itself must, onno account, be autoctaved, butmay be disinfected by wrpingdown with ajcohol or commercialdisinfectants.

~

4.3. Switching theInstrument On

The instrument is switched onby pressing the [ON/OFF] keyonce. The instrument is then instandby mode, indicated by thegreen LED in the upper part ofthe keypad.

4.2. Inserting the Agar Strip

Using aseptic technique. peelback the wrapper by about 4 cmand remove the agar strip.

Insert the agar strip into theslot in the open-end drum with

4.5. Starting the Instrument

After the required samplingtime has been set, the RCS AirSampler can be started either bypressing the [ON/OFF] key or byusing the infra-red remote control(Art. No. 940 341) within an ope-rating range of 10m.. The instru-ment can only be started usingthe remote control when it is instandby mode, i.e. the greenstandby LED is 1ft.

When the green LED blinks.this indicates that a measure-ment is in progress.

4.4. Setting theSampling Time

The instrument allows the useof anyone of 5 permanently pro-grammed sampling times: 0.5min, 1 min, 2 min, 4 min and 8min. The current sampling ttme isindicated by a green LED next tothe selected sampling time. Thesampling time is selected bypressing the [SELECl1 key. Eachtime this key is pressed, the nextsampling time will be selected inturn.

The sampling times corre-spond with the following sam-pling volumes:

4.6. Stopping the Instrument

The measurement is stoppedautomatically after completion ofthe selected sampling time. Ifrequired. sampling can be inter-rupted by pressing the [ON/OFF]key during measurement or byusing the remote control.

The instrument will switch it-self off automatically four minutes

~

5. Evatua t io n.""

Microbia] colonies are enumera-ted after incubation by vlsuaJexamination directly through thesealed ~gar strip wrapper. Themicrobial count (CFUs) can becaJcutated as foltows:

after completion of the measure::-ment or after the last time a keywas pressed, and the LED indi-cators will extinguish.

When the instrument is nextswitched on, the last selectedsampling time win be displayedautomatically.

4.7. Removing the Agar Strip

The agar strip is removed fromthe impeller drum by means ofthe grip tab. Replace the agarstrip, nutrient surface facingdownward, in its protective wrap-per. Then seal the wrapper withone of the slide seals provided orwith an adhesive tape to preventthe media from drying Ocut duringincubation.

To prevent any ccconfusion,labcel the agar strip wrapper withthe main sample data.

~~~~~~~~

not. light. up after pressing the[ON/OFF] key. Depending on thesampjing times used, up to 100measurements can be made withone set of batteries.

F~!..Jlts are indicated by means ofred LEOs..

blc: This LED lights up to indIcatethat the impeJler bJades havebeen obstructed during startingthe measurement. After correc-ting the obstruction I the indicatormust be cancelted by pressIngthe [RESEll key.

tt is recommended that thefollowing items be checked regu-larly:

- battery power- angle of the impeller blades- sampling time

bat: This LED lights up to indicatethat the battery voftage is toolow.. After replacing the batteriesand switching the instrument onagain by pressing the [ON/OFF]key, the indicator must be can-celled by pressing the [RESEllkey. If a measurement was inter-rupted because of row batteryv01tage, it must be repeated..

All three parameters affect theaccuracy 'of the separation volu-me. The checks described in thefollowing paragraphs are easy toperform and shouJ.d be used as abasis for your own »StandardOperating Procedure« (SOP) forthe RCS instrument.

71 Battery .Power. . .

7

The RCS Air Sampter has nospec.ial care or maintenancerequirements apart from regularbattery replacement. The batte-ries must be replaced when thered LED next to »bat« lights up orif the green standby LED does

When the instrument is swit-ched on, the green LED is lit. Ifth.is Indicator does not light up,then it is highly probable that thebatteries are exhausted. If the redLED next to »bat« tights up afterswitching on the instrument viathe [ON/OFF] key, this a.lsomeans that the battery vortage isinsufficient.

I.n either case, the batteriesmust be repl.aced and the instru-

7.3. Sampling Time'"

"'!

The average rotation frequen-cy of the RCS is 4096 rpm withan accuracy of :t: 2%. The adjust-ment of the running time of theunit is carried out by electronicregulation. Each revolution of theRCS generates 16 electronic'"impulses, which are counted.The tota.! number of impulses foreach running time is program-med in the unit. Deviations can"'be easily detected using a stop-watch",. The test procedure i",s asfollows: The running time is set at8 min",utes in ord",er to minimizethe error rate. Simu..ltaneously, the[ON/OFF]-button of the RC",S andthe start button of the stopwatchare pre",ssed. Wh",en the unit swit-ches off, stop", the stopwatch.WithJn the a",ccuracy of :t: 2%, theresult should not exeed or beless than 480 :t: 9.6 seconds. Ifth",e result is not within the limit,th",e batteries should be changedand the proce",dure repeated. Ifthe res",ult is still outside the limit,the instrument must be sent infor an electronic calibration.. It isrecommended to test monthly orafter each battery change.

ment checked once again. Caremust be taken to ensure that thebatteries are inserted lnto thebatte'ry compartment with thecorrect pol.ari~.

After replacing the batteriesand switch,ng the Instrument onvia the [ON/OFF} key, the red bat-tery alarm LED is cancelled bypressing the [RESEll-button andthe instrument is then ready foruse again.

7.2. Angle of Impeller Blades

Wi.th use of the unit the anglesof the impeller blades may be-come altered and correction tSrequired by use of the Cali.brationSet (Art. No.. 940 060). Theimpeller is ptaced into the Lowerpart (with imprinted article num-ber) of the cafibration set.. Theupper part of the set is then p!a-ced on the top so that the teethinterlock, thus ctosing the set. Bypressing firmly together, the posi-tion of the individual blades ischecked and if necessary correc-ted. ft is recommended to checkthe impe1fer b1ades once a week.

Art. No. 941 110 Agar Strips TC-y (twin pack, gamma-irradiated Tryptic Soy Agarfor determination of total airborne microbial count)

Art. No. 941 400 Agar Strips S (Mannitol Salt Agar for determination of

staphy1ococci)

Art. No. 941 700 Agar Strips PEN (determination of total airborne microbial countin air containing penicillins)

Art. No. 940020 Floor plate

9. Technical Data

Memory locations:5 memory locations for 0.5, 1,2.4 and 8 minutes. in ascendingorder.

Sampling principle:The Biotest. Standard RCS AirSampler operates on the princi-ple of impaction, whereby the airstream enters the unit at the frontof the instrument and the airbor-ne microorganisms are separa-ted out onto the agar strip bycentrifugal force.

Motor speed:4096 min (rpm)

Instrument deviation::t:2%

Measurement range:min. 20 titers, max. 320 liters

Power supply:4 x 1.5 Volt monocells (aJkaline 0cells, type L.R20) or mains adap-ter 230 Volt /110 Volt (Art. No.940 040 / 940 04 1

Air flow-rate (SeparationVolume):40 I/min

8ectrical power:3W

Materials:. Housing: chromfum~plated

brass. Keypad: polyester; resistant to

common disinfection agents. Impeller blade: aluminum;

autoclavable. Impeller drum: chromium-

plated brass; autoclavable

M iscellaneou s:. Screw thread connection fo r

floor plate. Infrared remote control sensor. Keypad with pressu re keysand LED indicators. LED Indicator for batteryvoltage

. Protection cap made frompaM; autoclavable

. Connection for mains adapter

. CE",Mark

Weight.:approx 1 ,.300 9 (2.9 Ibs) withbatteries

the use of parts from othermanufacturers, or through repairsor modifications carried out. bypersons not authorized byBiotest. to carry out such work.

Biotest declares its instrumentsto be free from defects of materl~als and workmanshi.p. The perIodof warrant')i is one {1) year com-mencing on the date of commis-sioning of the instrument. as evi-denced by means of an invoiceor other document.

3. Defects which occur as aresutt of normal. wear and tear offrom using the equipment underconditions not specrfied in theinstructions for use.

The user is not entitled to anyremedy in law towards Btotestthowsoever arising, apart fromthose remedies mentioned here.Biotest accepts no other liabilityor warranty towards the user. Thelegal righ1s of the purchasertowards the seller remain unaf-fected by this.

The warranty covers the rec-tification of all faults which occuras a proven direct. result of tautlymaterials or workmanship duringthe warranty period.

The folJowing items are not.covered by the warranty:

1. Parts subject to normal wearand tear.

2 . Malfunction caused by acci-dental or intentfonal damage1 or

~2