Spectro–Microscopy of Autofl uorescent Biomolecules

RHOMBOS

Sébastien Peter

RHOMBOS

LIFE SCIENCES

Sébastien Peter

Spectro–Microscopy of Autofl uorescent Biomolecules

182 Seiten. Zahlreiche zum Teil farbige Abbildungen. Hardcover, Preis: 44,00 Euro. ISBN 978-3-944101-99-6.Rhombos-Verlag, Berlin 2014

9 9Forschung & Entwicklung

LIFE SCIENCESForschung & Entwicklung

Sébastien Peter

Spectro–Microscopy of A

utofl uorescent Biom

olecules

Fluorescence microscopy has become an indispensable tool in life sciences. � e information within a microscopic image can be largely extended by the simultaneous acquisition of spectral information from a microscopic volume because � uorophores act as sensors to their nano-environment. In this work, di� erent classes of auto� uorescent biomolecules are spectro–microscopically investigated. It is shown that the photophysical properties of GFP derivatives largely depend on the composition of the surrounding protein shell, allowing for a better understanding of these proteins. Furthermore, the spectro–microscopic investigation of photosystems in living plant cells enables the observation of adaptation processes of plants to external and internal growth conditions. � e parallel implementation and establishment of novel spectro–microscopic modalities further extends the accessible parameter space, o� ering even deeper insights into the photophysics of auto� uorescent biomolecules.

Key words: auto� uorescent biomolecules, spectral information

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