SouthCarolinaDepartmentofNaturalResourcesMarineAquacultureProgram

• FortJohnsonCampus,CharlestonSC• MarineResourcesResearchInstitute• HollingsMarineLaboratory• WaddellMariculture Center,Bluffton

FortJohnsonCampus,MarineResourcesCenter

MarineResourcesResearchInstitute(MRRI)

AquacultureandStockEnhancementTeam

AquacultureandStockEnhancementTeam

• Stockenhancementofmarinespecies• Environmentaltolerancetesting• Fishnutrition/feeddevelopment• “omics”fordietformulationandtesting• Demonstration-scaletestingofdietsandtreatments• Genetictoolsforaquaculture• ApplicationofNanobubbletechnology• Effectsofparasitesonperformanceandfleshquality

WMCFacilitiesandResearch

Dr.AaronWatsonandCollegeofCharlestonstudentDavidKlett

SouthCarolinaDepartmentofNaturalResources– Mariculture Section-AquacultureNutritionResearch• Developandevaluatefishmealandfishoilreplacementfeedsandingredients• Utilizetraditionalaquacultureproductionmetricsaswellasadvancedmetabolictechniques• Increasedutilizationofmoresustainableandcost-effectiveingredientstoreplacefishmealandfishoil• Evaluateenzymeadditionsinplant-basedfishfeedstoincreasemineralutilization• Indigestiblephytase-bondphosphorusinplant-basedfeeds• Reduceeffluentwastewaterbyaddingphytaseenzymesallowfishtoefficientlyutilizephosphorus• Useofalternativefeedingredients• Brewerswasteasfeedadditive

Broodstock ConditioningCultureatMRRI

WMCFacilitiesandResearch

HollingsMarineLaboratory

WMCFacilitiesandResearch

WMCFacilitiesandResearch

Dr.FabioCasu

SouthCarolinaDepartmentofNaturalResources– MaricultureSection- AquacultureNutritionNMRbasedMetabolomicsresearch

• Increaseunderstandingofnutritionalrequirementsthroughmetabolicprofiling• ApplyNMR-basedmetaboliteprofiling

andLCMSOrbitraplipidprofilingtoidentifynutritionalneedsinavarietyoffeeds,species,andlifestages

• Develop“-Omics”toolsforaquacultureresearch• Developmentofbiomarkerdiscovery

methodsandnovelmetabolismandphysiologyevaluationtechniques

• Leveragecutting-edgetechnologytoidentifyanddevelopmorerapidtechniquestodiagnosenutritionaldeficiencies

WMCFacilitiesandResearch

Dr.TanyaDarden

SouthCarolinaDepartmentofNaturalResources– GeneticsResearch

• Usinggenetictoolstooptimizehatcheryproductionandsourcingbroodstock

• UsingGeneticsandIBMtodetermineriskofnetpenescapement

• UsingGeneticsandIBMstooptimizehatcheryproductionanddeterminestockinggoalsforstockenhancement

• Genetictoolstotrackfishreleasedintothewild

• Genetictoolsforforensicevaluationofseafoodproducts

• eDNAresearchtolocatebroodstock,invasivespeciesdetection

NIST/NOAA/CollegeofCharleston/CitadelMilitaryCollege

Dr.PeterMoeller

Dr.JodyBeersDr.PeterLee

Dr.EricMcElroy

Dr.MikeJanech

Dr.Isaure DeBuron

Dr.ClintonMoran

Dr.TraceySchock

WMCFacilitiesandResearch

WMCFacilitiesandResearch

ErinLevesqueandDr.JasonBroach

SouthCarolinaDepartmentofNaturalResources– MaricultureSection– WaddellMaricultureCenter

• StockEnhancementResearch• Improvespawningandlarvalandjuvenile

grow-outtechniquesforreddrum,spottedseatrout,andcobia

• Evaluateaquaculturepotentialandecologyofotherspeciesofrecreational,commercial,andconservationinteresttoSouthCarolina

• EducationandoutreachtothepublicregardingmarineresourcesandmaricultureeffortsinSouthCarolina

IntensiveAquacultureResearchWMC

• Growout systems• 4,6-tanksystems

• 12,1,250-Ltanks• 12,2,400-Ltanks

• Plumbingalsoadaptableforsingle,interconnected24-tanksystemthatallowsforincreasedreplicationwithreducedbackgroundvariation

• Evaluationofstockingdensities,temperatureeffects,feedrates,diets,etc.

• Plumbingadaptabletoallowforexternaleggcollectorsandreplicatedspawningexperimentsforsmall-bodiedfishes

IntensiveAquacultureResearchWMC

• Egg/Larval/ZooplanktonSystem• 20,155-LTanks• 5,1,063-LTanks• Eggincubation• Masscultureoffishlarvae• Replicatedevaluationsofenvironmentalandnutritioneffectsonlarvae

• Plumbingallowsforisolationofindividualtanksforzooplanktonculture(rotifersandArtemia)

ExtensiveCulture

• 12,0.25-acreponds• 9,0.5-acreponds• 3,1.25-acreponds

• Growout ofjuvenilesandsub-adultsforstockenhancementandtaggingstudies

• Commercial,pilot-scaletestingforevaluatinggrowout methodsandfeasibility

• Demonstrationscaleresearch

FutureResearchProjectsFunded

• ReplacingArtemia UseinLarvalFeedingRegimesofThreeMarineBaitfish- Spot,Pinfish,andPigfish• EvaluatingShellfishPondstoProduceaCompatibleCropofMarineFish,TripletailLobotes surinamensis

Opportunities

• Asaconsequenceofproducingfishforstockenhancementthereisoftenexcessfish• Keysoutheastspeciesavailable• Demonstration-scalefacilities• Accesstotoolsandtechniquestohelptheaquaculturecommunity• SBIRpartnerships• CooperativeExtensionprojects• Trainingopportunities• Geneticsampleprocessing• Consulting

• KeyPartners• State/federalnaturalresourceagencies(NC,GA,MD),Universities(CofC,Clemson,USC,UGA,VCU,WVU)

• Zeigler- RedDrumDigestibility(novelingredients)andGrowthTrial• Zeigler- ReductioninFishmeal/effluentwasteforRedDrum• IchthusUnlimited– NovelTunaDietNMR-basedMetabolomics• IchthusUnlimited(pending)– RedDrumDigestibility(novelingredients)

• BarronAlgae-algaeproductionasfood/animalfeedadditive• Nanopure Technology-Nanobubbleozonation• HubbsSeaWorldResearchInstitute• CharlestonBrewmasters• SwimmingRockfishandShrimpFarm• SouthlandFisheries• HamptonRoadsSanitationDistrict

Collaborations(Contractualfunding,SBIR,Jointcompetitivegrants)

• Useofpublicresourcesforprivateprofit• Easierforustopartnerwithorganizationsratherthancompanies• Non-DisclosureAgreements/IntellectualProperty• Cost(Collaborativegrant-basedmodelworksbest)• Space(hasn’tbeentoomuchofaproblem)• Risk• Competition• Timetomanagetherelationship• Safety• IACUC

ChallengestoPublic-PrivatePartnerships

• SaltwaterRecreationalSaltwaterLicenseFunds• SportfishRestorationFunds• SoyAquacultureAlliance• WaddellMariculture CenterFund• StateofSouthCarolina• HollingsMarineLaboratory• GriceMarineLaboratory

• SCDNRAquacultureTeam

Acknowledgements