South Asia Biosafety Conference September 18 19, 2013...
Transcript of South Asia Biosafety Conference September 18 19, 2013...
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South Asia Biosafety ConferenceSeptember 18‐19, 2013
Taj Ambassador Hotel, New Delhi, India
Dr. Thiago SouzaEmbrapa Rice & BeansSanto Antônio de Goiás, GO, [email protected]+55 (62) 3533‐2129+55 (62) 8127‐0254
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Considerations for the Commercialization of Embrapa’sVirus Resistant Common Bean (Embrapa 5.1)
Overview on the common bean crop in Brazil
Brief history of the Embrapa 5.1 technology
Stewardship Program
Post‐approval field trials for cultivar registration
Seed production (breeders seed, pre-basic and basic seed)
Technical perspectives on commercial release
Outline
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Dry Beans ‐ Brazil
Brazil is the second largest producer of dry beans in the world after India
Production in 2011 was estimated at 3.43 million tonnes (US$ 1.98 billion)
FAOSTATS
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Source: IBGE (2012), modified by Embrapa Rice and Beans.
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Some Embrapa’s common bean cultivars
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• Losses in grain production may range from 40 to 100% / 90‐to‐280,000 tonnes per year.• Annual losses would be enough to feed from six to 20 million of adult Brazilians.• At least 200,000 ha became impractical for bean growth in Brazil due to the BGMV; about 10% of the total common bean growing area.
BGMV ‐ Bean Golden Mosaic Virus
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Deformation of pods and grains
BGMV ‐ Bean Golden Mosaic Virus
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This geminivirus is transmitted by the whitefly (Bemisia tabaci)
BGMV ‐ Bean Golden Mosaic Virus
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Santo Antônio de Goiás, GO, Brazil – March 2013
Severe foliar yellow mosaic symptoms
Effective resistance has not been identified in P. vulgaris germplasmover 40 years of conventional breeding efforts in Brazil
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Embrapa ‐ Brazilian Agricultural Research Corporation, Brazilian Government.
Dr. Francisco AragãoEmbrapa Cenargen
Dr. Josias FariaEmbrapa Rice & Beans
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RNAi Technology
d 35S CaMV nosTintron antisensesense
alça
No new protein
Aragão & Faria (2009) Nature Biotechnology 27: 1086‐1088.
Embrapa’s BGMV Resistant Bean ‐ Event Embrapa 5.1
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Transformation Process ‐ Event Embrapa 5.1Method patented by Embrapa
PARTICLE BOMBARDMENT OR BIOLISTICS
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Official Biosafety Assessment
CTNBio ‐ National Technical Commission on Biosafety Multidisciplinary consultative body established under the Ministry of Science and Technology to provide technical and advisory support to the Federal Goverment for
the implementation of the national biosafety policy
Brazilian Biosafety Policy Law (Law No. 11,105/2005)
Molecular characterization Agronomic characterization Environmental risk assessment Nutritional composition analysis Food security assessment
Event Embrapa 5.1
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Biosafety Assessment Project
%
Production of common bean (Phaseolus vulgaris L.) crop in Brazil per State
Leader: Dr. Josias FariaEmbrapa Rice & Beans
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Commercial Approval ‐ Sep. 16, 2011CTNBio: http://www.ctnbio.gov.br/
Embrapa’s BGMV Resistant Bean ‐ Event Embrapa 5.1
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Cristalina, GO, Brazil – 15/Aug/2013
Field Meeting ‐ Common Bean Farmers
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Second largest (by volume) producer of transgenic crops in the world, in 2012 Brazil planted over 36 million ha of transgenic soybean, corn and cotton.
Source: James (2012).
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Brazilian Commercial Approvals(up to September 2013)
TOLERANCE TO HERBICIDES AND INSECT
Soybean: 5 traits
Corn: 19 traits
Cotton: 12 traits
Common bean: Embrapa 5.1
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Brazil ‐ Very Attractive Seed MarketEstimated at over US$ 2 billion (third largest market in value)
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BC ProgramTransgene Estability
Credited to Dr. Josias C. Faria
Olathe GM Pérola and BRS Pontal
Event Embrapa 5.1
Backcrossing (BC) Program
Embrapa 5.1 BGMV Resistant BeanCultivar Development
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# Genotype Embrapa code Note1 1-4-8-2-3-NIL PÉROLA CNFCT 16201 Transgenic2 1-10-6-1-2-NIL PÉROLA CNFCT 16202 Transgenic3 1-16-10-2-NIL PÉROLA CNFCT 16203 Transgenic4 10-5-6-1-2-NIL PÉROLA CNFCT 16204 Transgenic5 094-NIL Pontal CNFCT 16205 Transgenic6 097-NIL Pontal CNFCT 16206 Transgenic7 107-NIL Pontal CNFCT 16207 Transgenic8 125-NIL Pontal CNFCT 16208 Transgenic9 132-NIL Pontal CNFCT 16209 Transgenic
10 138-NIL Pontal CNFCT 16210 Transgenic11 Pérola BGF7384 Conventional12 BRS Pontal CNFC7813 Conventional13 IPR Eldorado IPREldorado Conventional14 BRB 169 BGF8419 Conventional15 CNFP 15882 CNFP15882 Conventional
Final field trials
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Embrapa’s Units: 1) Arroz e Feijão2) Trigo3) Milho e Sorgo4) Agropecuária Oeste5) Cerrados6) Tabuleiros Costeiros7) Rondônia8) Soja9) SPM (6 ETs)10) CTAA11) Semi-Árido
Partners:1) UFLA2) UFV3) UFU4) UFG5) Epamig6) Empaer MT7) EBDA8) IPA9) Cooperativas e Consultores
Final field trials to evaluate the agronomic performance of the bean transgenic linesNational Assay Network
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Ensure that “Embrapa 5.1 Technology" WILL NOT get in the market BEFORE its COMMERCIAL RELEASE
Stewardship Program
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Stewardship Program
Detailed guidance on best practices to ensure containment, segregation, control and traceability
of transgenic materials
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Trainings on best practices for different activities
Stewardship Program
Almost 100 employees trained since 2011
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Transgenic seed collection (seed bank)
Stewardship Program
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GPS coordinates
10m
Field Trials ‐ Sowing
Stewardship Program
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Field Trials ‐ Harvesting
Stewardship Program
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Stewardship ProgramClean‐up Protocol
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# Loc Season Trial ID Location State
1 First/2012 F11VCT001 Santo Antônio de Goiás GO2 First/012 F11VCT002 Anápolis GO3 Second/2012 F11VCT003 Santo Antônio de Goiás GO4 Third/2012 F11VCT005 Sete Lagoas MG5 Third/2012 F11VCT007 Ponta Grossa PR6 Third/2012 F11VCT008 Santo Antônio de Goiás GO7 Third/2012 F11VCT009 Brasília DF8 First/2013 F11VCT011 Londrina PR9 First/2013 F11VCT013 Santo Antônio de Goiás GO10 First/2013 F11VCT012 Brasília DF11 First/2013 F11VCT006 Sete Lagoas MG12 First/2013 F11VCT010 Dourados MS
Final Field Trials ‐ Finalized
Three field trials in course - Season: Second/2013
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# Genotype Embrapa code Note Status1 1-4-8-2-3-NIL PÉROLA CNFCT 16201 Transgenic Potential2 1-10-6-1-2-NIL PÉROLA CNFCT 16202 Transgenic No selected3 1-16-10-2-NIL PÉROLA CNFCT 16203 Transgenic No selected4 10-5-6-1-2-NIL PÉROLA CNFCT 16204 Transgenic SELECTED5 094-NIL Pontal CNFCT 16205 Transgenic Potential6 097-NIL Pontal CNFCT 16206 Transgenic Potential7 107-NIL Pontal CNFCT 16207 Transgenic SELECTED8 125-NIL Pontal CNFCT 16208 Transgenic Potential9 132-NIL Pontal CNFCT 16209 Transgenic Potential
10 138-NIL Pontal CNFCT 16210 Transgenic No selected11 Pérola BGF7384 Conventional12 BRS Pontal CNFC7813 Conventional13 IPR Eldorado IPREldorado Conventional14 BRB 169 BGF8419 Conventional15 CNFP 15882 CNFP15882 Conventional
Final Field Trials ‐Main Conclusions
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Ponta Grossa, PR, Brazil – December 2012
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Santo Antônio de Goiás, GO, Brazil ‐ August 2013
Seed Production is on Schedule(breeders and pre‐basic seed)
Technical target for the commercial release: 2015/2016 ‐ 50,000 ha (25%)
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Handle large growing areas for agronomic performance observation and certified seed production following the Stewardship Program is
challenging…
We’re still working on the business plan for the Embrapa 5.1 technology...
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Backcrossing (BC) Program Assisted byMolecular Markers
Doador x Recurrent
4 months
PCR F1 x Recurrent
4 months
PCR BC1F1 x Recurrent
4 months
PCR BC2F1 x Recurrent
4 months
PCR + FP BC3F1 >99% (93,75%)
4 months
PCR BC3F2 Homozigosity
4 months
BC3F2:3
24 months
%GR = 100 x [1‐(0,5)n+1]
Development of new transgenic bean cultivars
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Embrapa common bean cv. BRS Estilo
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Research ScientistAdriane WendlandHelton PereiraLeonardo MeloLuis Cláudio de FariaThiago Souza
Senior Research AssistantJose Luis CabreraMariana CruzickNilda Pessoa
Research AssistantJoão Donizete PuríssimoMarcos AtaídePaulo FernandoVicente Henrique
Embrapa’s Common Bean Breeding Team
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Thank you!Dr. Thiago SouzaEmbrapa Rice & BeansSanto Antônio de Goiás, GO, [email protected]+55 (62) 3533‐2129+55 (62) 8127‐0254