SOLAµ increasing LC-MS...
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thermoscientific.com/sola-spe - Getting more from your sample SOLAµ increasing LC-MS sensitivity Sample Preparation Sample Handling LC Columns and Accessories Separation Detection Data Processing A comprehensive product offering for your complete bioanalytical workflow Ordering information Description Part Number SOLAμ HRP 96 well plate 60209-001 SOLAμ SCX 96 well plate 60209-002 SOLAμ SAX 96 well plate 60209-003 SOLAμ WCX 96 well plate 60209-004 SOLAμ WAX 96 well plate 60209-005 Complementary products Description Part Number HyperSep-96 Well Plate Manifold 60103-351 Vacuum Pump, European Version 60104-241 Vacuum Pump, North American Version 60104-243 © 2015 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details. USA and Canada +1 800 332 3331 Australia 1300 735 292 (free call domestic) China 800 810 5118 (free call domestic) 400 650 5118 PO21202-EN 0515S France +33 (0)1 60 92 48 34 Germany +49 (0) 2423 9431 20 or 21 India +91 22 6742 9494 +91 27 1766 2352 Japan 0120 753 670 (free call domestic) 0120 753 671 (fax) Korea +82 2 3420 8600 United Kingdom +44 (0) 1928 534 110 New Zealand 0800 933 966 (free call domestic) Singapore +65 6289 1190 All Other Enquiries +44 (0) 1928 534 050 Technical Support For advice and support, please visit our website: www.thermoscientific.com/chromexpert No compromise The SOLAµ family consists of a wide range of phase chemistries suitable for almost every compound type. This allows for extremely selective analysis removing potential interferences. As a result increased concentration can be achieved without increased matrix effects. Thermo Scientific ™ SOLAμ ™ is a micro elution device built on Embedded Particle Technology (EPT), which removes the need for frits and so eliminates issues associated with traditional loose-packed SPE. This results in highly reproducible, accurate data. SOLAμ enables: • An increase in your sensitivity by up to 20 fold • Removal of evaporation and reconstitution steps • Removal of analyte volatility, adsorption and solubility issues Simple workflow Exchanging your traditional SPE for SOLAµ allows you to keep the workflow simple. By reducing your elution volumes you can directly inject the eluent with no further processing. This can provide up to 20-fold increase in sample concentration with no additional steps. Make your sample work harder Low elution volumes gives you the tools to concentrate your sample pre-analysis without any need for evaporation. This not only saves you time but avoids issues with loss of volatile compounds, or compounds susceptible to non-specific binding such as proteins and peptides. • SOLAµ: sample enrichment 20 × preconcentration) • Observed matrix effects on a selection of opiates extracted from human urine using SOLAµ SCX method and analysed by LC-MS/MS with electrospray ionization. 1:1 dilution with 4% H 3 PO 4 SOLAμ 20 pg/mL 25 μL elution 800 pg/mL 1:1 dilution with 4% H 3 PO 4 1:10 dilution with 4% H 3 PO 4 Conventional scale SPE 20 pg/mL 500 μL elution 40 pg/mL Dry down Re-con in 25 μL 800 pg/mL Conventional scale SPE 20 pg/mL 40 pg/mL 500 μL sample 40 pg/mL 500 μL sample 40 pg/mL 500 μL sample 500 μL elution 40 pg/mL Problem; improvement in assay sensitivity required Option 2; SOLAμ provides twenty fold increase in sample concentration without changes to workflow Option 1; introduce additional steps with associated issues 0% -5% -10% -15% -20% -25% -30% -35% -40% -45% -50% -55% -60% -65% -70% cis-tramadol codiene EDDP fentanyl hyrdoocodone hydromorphone meperidine methadone morphine northydrocodone noroxycodone O-desmethyltramadol % Ion Suppression Time (Minutes) 0 0.5 1.0 1.5 2.0 2.5 3.0 10 20 30 40 50 60 70 80 90 100 Relative Abundance 1.33 1.35 1.36 1.36 1.38 1.31 0.96 1.48 1.81 2.28 1.96 0.73 0.51 2.51 0.22 2.77 1.33 1.34 1.33 1.36 0.97 0.52 2.16 1.31 2.24 2.53 1.55 1.88 0.75 2.82 0.27 0.14 10 20 30 40 50 60 70 80 90 100 Peak Area 144300 counts Peak Area 7215 counts 400 pg/mL aqueous solution of niflumic acid injected neat (no concentration factor) 400 pg/mL aqueous solution of niflumic acid taken through the extraction procedure (20 × concentration factor)
Transcript of SOLAµ increasing LC-MS...
thermoscientific.com/sola-spe
- Getting more from your sampleSOLAµ increasing LC-MS sensitivity
Sample Preparation Sample Handling LC Columns and Accessories
Separation Detection Data Processing
A comprehensive product offering for your complete bioanalytical workflow Ordering informationDescription Part Number
SOLAμ HRP 96 well plate 60209-001 SOLAμ SCX 96 well plate 60209-002 SOLAμ SAX 96 well plate 60209-003 SOLAμ WCX 96 well plate 60209-004 SOLAμ WAX 96 well plate 60209-005 Complementary products Description Part Number
HyperSep-96 Well Plate Manifold 60103-351 Vacuum Pump, European Version 60104-241 Vacuum Pump, North American Version 60104-243
© 2015 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details.
USA and Canada +1 800 332 3331
Australia 1300 735 292 (free call domestic)
China 800 810 5118 (free call domestic) 400 650 5118
PO21202-EN 0515S
France +33 (0)1 60 92 48 34
Germany +49 (0) 2423 9431 20 or 21
India +91 22 6742 9494 +91 27 1766 2352
Japan 0120 753 670 (free call domestic) 0120 753 671 (fax)
Korea +82 2 3420 8600
United Kingdom +44 (0) 1928 534 110
New Zealand 0800 933 966 (free call domestic)
Singapore +65 6289 1190
All Other Enquiries +44 (0) 1928 534 050
Technical SupportFor advice and support, please visit our website: www.thermoscientific.com/chromexpert
No compromiseThe SOLAµ family consists of a wide range of phase chemistries suitable for almost every compound type. This allows for extremely selective analysis removing potential interferences. As a result increased concentration can be achieved without increased matrix effects.
Thermo Scientific™ SOLAμ™ is a micro elution device built on Embedded Particle Technology (EPT), which removes the need for frits and so eliminates issues associated with traditional loose-packed SPE. This results in highly reproducible, accurate data. SOLAμ enables:
• An increase in your sensitivity by up to 20 fold
• Removal of evaporation and reconstitution steps
• Removal of analyte volatility, adsorption and solubility issues
Simple workflowExchanging your traditional SPE for SOLAµ allows you to keep the workflow simple. By reducing your elution volumes you can directly inject the eluent with no further processing. This can provide up to 20-fold increase in sample concentration with no additional steps.
Make your sample work harderLow elution volumes gives you the tools to concentrate your sample pre-analysis without any need for evaporation.
This not only saves you time but avoids issues with loss of volatile compounds, or compounds susceptible to non-specific binding such as proteins and peptides.
• SOLAµ: sample enrichment 20 × preconcentration)
• Observed matrix effects on a selection of opiates extracted from human urine using SOLAµ SCX method and analysed by LC-MS/MS with electrospray ionization.
1:1 dilutionwith 4% H3PO4
SOLAμ
20 pg/mL 25 μLelution
800 pg/mL
1:1 dilutionwith 4% H3PO4
1:10 dilutionwith 4% H3PO4
Conventionalscale SPE
20 pg/mL 500 μLelution
40 pg/mL
Dry down
Re-conin 25 μL
800 pg/mL
Conventionalscale SPE
20 pg/mL
40 pg/mL500 μL sample
40 pg/mL500 μL sample
40 pg/mL500 μL sample
500 μLelution
40 pg/mL
Problem;improvement in assay
sensitivity required
Option 2; SOLAμ providestwenty fold increase insample concentration
without changes to workflow
Option 1; introduceadditional steps withassociated issues
0%-5%
-10%-15%-20%-25%-30%-35%-40%-45%-50%-55%-60%-65%-70%
cis-tr
amad
ol
codie
ne
EDDPfen
tanyl
hyrd
ooco
done
hydr
omor
phon
e
meperi
dine
methad
one
morph
ine
north
ydro
codo
ne
noro
xyco
done
O-des
methylt
ramad
ol
% Io
n S
uppr
essi
on
Time (Minutes)
0 0.5 1.0 1.5 2.0 2.5 3.0
102030405060708090
100
Rel
ativ
e Ab
unda
nce
1.33
1.351.361.361.381.310.96 1.48 1.81 2.281.960.730.51 2.510.22 2.77
1.33 1.341.331.360.970.52 2.161.31 2.24 2.531.55 1.880.75 2.820.270.14
102030405060708090
100
Peak Area144300 counts
Peak Area7215 counts
400 pg/mL aqueoussolution of niflumic acidinjected neat
(no concentration factor)
400 pg/mL aqueoussolution of niflumic acidtaken through the extractionprocedure
(20 × concentration factor)
