soj (parA) - bioinfo.sfc.keio.ac.jp · Soj proteins were detected by western blot analysis. The top...

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Transcript of soj (parA) - bioinfo.sfc.keio.ac.jp · Soj proteins were detected by western blot analysis. The top...

Page 1: soj (parA) - bioinfo.sfc.keio.ac.jp · Soj proteins were detected by western blot analysis. The top panel shows the amount of each Soj protein in the lysate following crosslinking
Page 2: soj (parA) - bioinfo.sfc.keio.ac.jp · Soj proteins were detected by western blot analysis. The top panel shows the amount of each Soj protein in the lysate following crosslinking

soj (parA):an ATPase that interacts with Spo0J

spo0J (parB):DNA-binding protein

parS:A specific DNA sequence motif(binding site for the Spo0J)

Page 3: soj (parA) - bioinfo.sfc.keio.ac.jp · Soj proteins were detected by western blot analysis. The top panel shows the amount of each Soj protein in the lysate following crosslinking

soj K16A:ATP binding deficient,cooperative DNA binding deficient

soj G12V:ATP binding proficient,cooperative DNA binding deficient

soj D40A:ATP2-bound dimer,cooperative DNA binding proficientATP hydrolysis deficient

Page 4: soj (parA) - bioinfo.sfc.keio.ac.jp · Soj proteins were detected by western blot analysis. The top panel shows the amount of each Soj protein in the lysate following crosslinking

http://homepages.strath.ac.uk/~dfs97113/BB310/Lect2/Lect2.html

Page 5: soj (parA) - bioinfo.sfc.keio.ac.jp · Soj proteins were detected by western blot analysis. The top panel shows the amount of each Soj protein in the lysate following crosslinking
Page 6: soj (parA) - bioinfo.sfc.keio.ac.jp · Soj proteins were detected by western blot analysis. The top panel shows the amount of each Soj protein in the lysate following crosslinking

During vegetative growth, the formation ofa Z-ring at midcell, and the subsequentassembly of the division machinery anddivision septum, leads to the formation oftwo equal daughter cells. By contrast,during sporulation, cell division isasymmetric, and unequal progeny — theforespore and the mother cell — areformed. Asymmetric cell division occursafter the transient formation of FtsZ spiralsdistributes FtsZ molecules to both poles ofthe cells, allowing the formation of an FtsZring at each pole. Only one of these formsa division or spore septum.

Page 7: soj (parA) - bioinfo.sfc.keio.ac.jp · Soj proteins were detected by western blot analysis. The top panel shows the amount of each Soj protein in the lysate following crosslinking
Page 8: soj (parA) - bioinfo.sfc.keio.ac.jp · Soj proteins were detected by western blot analysis. The top panel shows the amount of each Soj protein in the lysate following crosslinking

http://www.sci.hokudai.ac.jp/science/science/H13_05/koubunsi/cell-division.html

Normal Cell Division Potential Regions for Cell Division

Page 9: soj (parA) - bioinfo.sfc.keio.ac.jp · Soj proteins were detected by western blot analysis. The top panel shows the amount of each Soj protein in the lysate following crosslinking
Page 10: soj (parA) - bioinfo.sfc.keio.ac.jp · Soj proteins were detected by western blot analysis. The top panel shows the amount of each Soj protein in the lysate following crosslinking
Page 11: soj (parA) - bioinfo.sfc.keio.ac.jp · Soj proteins were detected by western blot analysis. The top panel shows the amount of each Soj protein in the lysate following crosslinking
Page 12: soj (parA) - bioinfo.sfc.keio.ac.jp · Soj proteins were detected by western blot analysis. The top panel shows the amount of each Soj protein in the lysate following crosslinking
Page 13: soj (parA) - bioinfo.sfc.keio.ac.jp · Soj proteins were detected by western blot analysis. The top panel shows the amount of each Soj protein in the lysate following crosslinking
Page 14: soj (parA) - bioinfo.sfc.keio.ac.jp · Soj proteins were detected by western blot analysis. The top panel shows the amount of each Soj protein in the lysate following crosslinking
Page 15: soj (parA) - bioinfo.sfc.keio.ac.jp · Soj proteins were detected by western blot analysis. The top panel shows the amount of each Soj protein in the lysate following crosslinking
Page 16: soj (parA) - bioinfo.sfc.keio.ac.jp · Soj proteins were detected by western blot analysis. The top panel shows the amount of each Soj protein in the lysate following crosslinking