Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through...

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Snail Model System

Transcript of Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through...

Page 1: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

Snail Model System!

Page 2: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

Intro to snails as a model system in neurobiology!

Our lab snail!

Page 3: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal
Page 4: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal
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Pond snail brains!

Page 6: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

ventral dorsal

Identified neurons in pond snail brains!

Page 7: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

Snail Buccal Ganglia

Page 8: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

Buccal ganglia neurons!

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Smail Week 1. Firing properties of snail neurons!

Page 10: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

Responses to current injection!

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Firing Accomondation!

Page 12: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

Post-inhibitory rebound!

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Burster neurons!

Page 14: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

Snail week 2. Ionic mechanisms of excitability!

Reduced Na! Reduced Ca!

Page 15: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

K+ Channel block with TEA!

Page 16: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

K+ Channel block with TEA/Cs!

Page 17: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

TEA/CS block progressing over time (5 minutes)!

Page 18: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

K+ Channel block with barium!

Page 19: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

Excitability mechanisms: Voltage Clamp!Describing the ionic conductances underlying the AP!

Page 20: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

Voltage clamp technique to examine ionic currents!

Page 21: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

Problems analyzing conductances underlying AP!

! 1. ICm obscured IRm!

! 2. Conductances changed during AP!

Current across membrane!

Im = I Rm + ICm!

Page 22: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

Ohm’s law for V-Clamp!

Page 23: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

Ionic driving forces at rest!

Page 24: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

Ionic driving forces at rest!

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Current across membrane with

hyperpolarizations!

Im = I Rm + ICm!

Page 26: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

Current across membrane with

weak depolarizations!

Page 27: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

Current across membrane with

strong depolarizations!

Page 28: Snail Model System - courses.cit.cornell.edu...5.1 Snail Brain ganglia pedal (to foot) (tube through Figure 5.1. Snail brain. The gut runs through the space between cerebral and subesophageal

INa!

IK!

Measure I!


Malika Snail Resume

Malika Snail Resume

Snail Game

Snail Game

Snail Quiz

Snail Quiz

No.2 high school of Hanshou. Snail Snail Tortoise.

No.2 high school of Hanshou. Snail Snail Tortoise.

Amigurumi Snail Eng

Amigurumi Snail Eng

The Cosmic Snail

The Cosmic Snail

Snail Montreal

Snail Montreal

ЦТИ "Snail"

ЦТИ "Snail"

Презантация ЦТИ "Snail"

Презантация ЦТИ "Snail"

Snail Farm

Snail Farm

Kyogen- The Snail

Kyogen- The Snail

Snail Management

Snail Management

Snail Farming Presentation

Snail Farming Presentation

Snail Mail Zine

Snail Mail Zine

Curios Snail

Curios Snail

Snail Dissertation

Snail Dissertation

Mattin the snail

Mattin the snail

BRERC Snail Key

BRERC Snail Key

Stephanie snail REUcon

Stephanie snail REUcon

Garden Snail

Garden Snail