REU TALK – June 14,2011 Signal Processing REU talk 14jun11 Phil Perillat.
Smith Lab REU Project - Michigan State University · Smith Lab REU Project: Research Toward...
1
Smith Lab REU Project: Research Toward Sustainable Enzymatic Catalysis We have shown that amphiphilic polymers do not aggregate, which allows the size of unimolecular micelles to be controlled by the molecular weight of the polymer. Our goals include (i) synthesizing amphiphilic polymers and studying how their backbone and sidechain compositions affect polymer properties, and (ii) assessing their ability to stabilize enzymes ex vivo. Enzymes are remarkable catalysts. Unimolecular micelles could stabilize enzymes ex vivo for catalyzing reactions where reactants and/or products are not biocompatible (e.g. the enzymatic epoxide resolution below). CuAAC, "click" Post-polymerization modification Functionalized polymer synthon Amphiphilic polymers Unimolecular micelles Insoluble globule > LCST < LCST no cmc H 2 O H O R n Hydrophobic azides Hydrophilic azides Encapsulated SC (SC@polymer) Subtilisin Carlsberg (SC) Unimolecular micelles are little explored amphiphilic materials containing hydrophobic and hydrophilic groups in the same molecule. Typical micelles self- assemble from several molecules. Success on this front would be an important step forward in sustainable catalysis. Students will learn: • Monomer and polymer synthesis • Post-polymerization modification • Purification techniques • Polymer characterization • Enzymatic catalysis O R R = Me, Ph O R (S) R OH HO (R) + Encapsulated Agrobactirium Radiobacter (AR@polymer) H 2 O AR@polymer Tunable LCST. Solubility decreases while the temperature increases
Transcript of Smith Lab REU Project - Michigan State University · Smith Lab REU Project: Research Toward...
SmithLabREUProject:ResearchTowardSustainableEnzymaticCatalysis
We have shown that amphiphilic polymers do notaggregate, which allows the size of unimolecularmicelles to be controlled by the molecular weight of thepolymer. Our goals include (i) synthesizing amphiphilicpolymers and studying how their backbone andsidechain compositions affect polymer properties, and(ii) assessing their ability to stabilize enzymes ex vivo.
Enzymes are remarkable catalysts. Unimolecularmicelles could stabilize enzymes ex vivo for catalyzingreactions where reactants and/or products are notbiocompatible (e.g. the enzymatic epoxide resolutionbelow).
CuAAC, "click"Post-polymerization
modificationFunctionalized
polymer synthonAmphiphilic polymers
Unimolecular micelles
Insolubleglobule
> LCST
< LCST
no cmc
H2O
HO
Rn
Hydrophobic azides
Hydrophilic azides
Encapsulated SC(SC@polymer)
Subtilisin Carlsberg(SC)
Unimolecular micelles are little explored amphiphilicmaterials containing hydrophobic and hydrophilicgroups in the same molecule. Typical micelles self-assemble from several molecules.
Success on this front would be an important stepforward in sustainable catalysis.
Studentswilllearn:• Monomerandpolymersynthesis• Post-polymerizationmodification
• Purificationtechniques• Polymercharacterization• Enzymaticcatalysis
O
RR = Me, Ph
O
R(S)
R
OHHO
(R)+
EncapsulatedAgrobactirium Radiobacter
(AR@polymer)
H2O
AR@polymer
TunableLCST.Solubilitydecreaseswhilethetemperatureincreases
