SMB_038 Using an X-ray power generator (PDF)

Creation date: 25/10/2012 Last review date: 27/3/2015

Next review due: 27/3/2017 of 4

Procedure: Using an X-ray generator School/Department: School of Molecular Bioscience SOP prepared by: Mitchell Guss and Nick Coleman Version: SMB038.3

Section 1 - Personal Protective Equipment

1. Lab Coat or Lab Gown 2. Proper enclosed footwear 3. Hair tied back if long

Section 2 – Potential Hazards + Safety precautions 1. This equipment is potentially hazardous, and must not be used unless you have been

trained by the equipment custodian 2. This equipment produces X-rays, which can cause burns or DNA damage leading to cancer.

This risk is minimal if the equipment is in good working order, and being used correctly by a trained operator.

3. Read the risk assessment for X-ray equipment and ensure you understand the risks before you start work. Consult your supervisor if you are unsure about the risks

4. The X-ray equipment contains very high voltages, which pose an electrocution risk. Ensure that water does not spill into the equipment.

5. If the machine is not working as expected, or any unusual phenomena occur, cease using it immediately and report to your supervisor and to the service centre/the manufacturer if faults are suspected

6. Do not disable or bypass safety circuits 7. Workers with pre-existing medical conditions (e.g. allergy, immunocompromised state, chemical

sensitivity) and workers who are pregnant or expecting pregnancy must consult with their supervisor AND medical specialist AND the university's WHS services before performing this procedure. If there are any serious concerns expressed by any of these individuals, this task must not be performed.

Section 3 – Equipment and Procedure Equipment

1. X-ray generator located in room 283, Chiller located in room 285 Powering up:

2. If generator is operating at normal power (40 kV, 30 mA) proceed to 8. 3. Check chiller in room 285 - if operating at 20 ˚C (See Figure A) proceed to 4. 4. Switch on chiller (Figure A), wait 3 minutes, if not at 20 ˚C report fault, get help. 5. Check vacuum in generator (Figure B(i); manual p 21) if not operating get help. 6. Switch on X-ray (ON switch on “X-RAYS” panel (Figure B(ii); manual p 21) 7. Raise the power to 40 kV and 30 mA slowly with the kV first – wait at least 30 minutes before

using. 8. Check appropriate cryosystem (if required). See separate SOP.

Using the X-rays:

9. Mount and centre crystal. 10. Shut all safety enclosure doors around the appropriate beamline. 11. Arm the shutter by pressing the 4 digit code on the safety panel (Figure C). 12. Open the shutter (1 or 2 as appropriate) on the generator “SHUTTER” panel (Figure B(ii)). 13. Check the level of X-ray flux. If less than value in log book, get help. 14. Fill in log book - collect data. 15. On completion of data collection, close the shutter and remove the crystal. 16. On completion check whether machine is to be shut down or left operating.

STANDARD OPERATING PROCEDURE



Figure A – Haskris chiller unit Chiller for Rigaku generator – located in room 285 (rear of X-ray laboratory) When operating correctly the 3 “operating lights” are on, the temperature actual (red) is 18.0 and the flow should be 5L/min.

Figure B(i) – Rigaku X-ray generator Located in room 283. Vacuum is operating normally when the two vacuum lights are on and the readout is less than 140 mV. Figure B(ii) – Rigaku X-ray generator Located in room 283 To switch on X-ray first check vacuum is operating normally. Then “On” switch of the “X-RAY ON” panel. Wait until voltage and current reach 20 kV and 10 mA. Any faults will be reported in the MODE panel. If there is a fault note the number and – get help.

Section 4 – Disposal / Spills / Incidents

1. Any incidents that involve large spills of cryogenic agents (>1 L) or injuries to personnel need to be reported immediately to your supervisor and via the online system. Near misses (situations that appear hazardous) should also be reported

Section 5 – Repairs / Certification / Validation 1. The X-ray generator, water cooler and X-ray optics are supplied by Rigaku and are covered by a

full (gold) warranty. They are represented in Australia by AXT but Rigaku are ultimately responsible for fixing all problems. Rigaku Americas 9009 New Trails Drive The Woodlands



Texas USA 77381-5209 email: [email protected] Tel: +1-281-362-2300 FAX: +1-281-364-3628 AXT Pty Ltd. Unit 1, 3 Vuko Place Warriewood NSW 2102 Australia Phone: 9450 2972; 9450 1359 Email: [email protected]; [email protected]

2. The detectors are supplied and serviced by Marresearch on an ad-hoc basis. The only routine maintenance is to check the filters in the scanner unit and to replace the erase lamps as required. Marresearch GmbH Hans-Böckler-Ring 17 D-22851 Norderstedt Germany Phone: +49 (40) 529 884-0 Email: [email protected]

Section 6 – Relevant safety data sheets Not applicable. Section 7 - References

1. Risk assessment for X-ray systems and Cryogenics 2. SOP for X-ray cryosystems (SMB037) and Cryogenics (SMB010).

SOP Consultation, Training and Approval

Print names and enter signatures and dates to certify that the persons named in this section have been consulted/trained in relation to the development and implementation of this Standard Operating Procedure. WHS Representative (WHS Committee) certifies that consultation has taken place.

Position Name Signature Date Supervisor employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student



employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student

Name Authorising (Printed): DIANNE FISHER ....................................................... Signature: .............................................................. Date: 27/3/15 ............................

WHS Committee Representative Name (Printed): MARKUS HOFER ................... Signature: .............................................................. Date: 27/3/15 ............................

SMB_038 Using an X-ray power generator (PDF)

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