Small Animal Imaging Poster - University of Sheffield · Doppler Flow Imaging Magnetic Resonance...
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Ultrasound Imaging Small Animal Imaging Facilities within University Departments - Towards a Core Facility Presented by FRIC Small Animal Imaging Working Group Doppler Flow Imaging Magnetic Resonance Imaging Bioluminescence/fluorescence Imaging Moor Instruments LD12-HR • Non-invasive laser doppler • Angiogenesis • Hind limb ischaemia • Spreading Cortical Depression Doppler Flow Images Control P2Y12 -/- Caliper Life Sciences IVIS Lumina II • Non-invasive bioluminescence • Non-invasive fluorescence • Cell trafficking • Gene therapy/expression • Tumor development & regression • Bioluminescent transgenic lines Intravital Microscopy Optical imaging of • Bioluminescence • Fluorescence • Nanoparticle • Cell tracking/adhesion • Blood velocity • Permeability Intravital imaging. (A) P22 rat sarcoma growing in a dorsal skin flap window chamber. Image was obtained using multiphoton fluorecence microscopy following i.v. administration of 70 kDa FITC- dextran. High spatial resolution is obtained and repeat imaging is possible. (B) Fluorescently labelled leukocytes can be used to assess rolling, adhesions and transmigration in post-capillary venules for the mouse cremaster muscle. Bruker 7 Tesla, 310mm Bore MRI with Oxford Instruments HyperSense DNP Polariser. • Non-invasive anatomical scans • Cardiac scans • Tumour development • Perfusion, oxygenation and metabolism • Neurological imaging Tumour metabolism. ( A ) 1H anatomical image of rat tumour. ( B ) Tumour uptake of hyperpolarised 13C-labelled pyruvate. (C) Tumour signals for pyruvate and lactate over time, which can be analysed to calculate rate constants. Contact: Claire Lewis & Gill Tozer Data provided by David Dockrell & Helen Marriott Contact: Sheila Francis Data provided by Sheila Francis, Tim Chico & Rob Storey Contact: Jason Berwick, Anneurin Kennerley, Martyn Paley & Gill Tozer Data provided by Steve Reynolds, Samira Kazan, Anneurin Kennerley & Allan Lawrie Contact: Gill Tozer, Nicky Brown & Vicky Ridger Data provided by Gill Tozer & Vicky Ridger P22 tumour Trafficking of S. pneumoniae. ( A ) Mice instilled with bioluminescent S. pneumoniae. Images show persistence of bacteria of the WT mice and shows how changes in bacterial burden can be followed in real time. (B) higher bacterial numbers in the blood (CFU/ml). A B A B C VisualSonics Vevo 770 • Non-invasive rodent imaging • Qualitative & Quantitative • Cardiac / vascular imaging • Echocardiogaphy • Tumor detection • Tumor quantification • Blood flow A 0 100 200 300 400 500 d21 after MCT Anti- OPG d21 after MCT Baseline IgG !-TRAIL !-OPG * LV CI (!l/min/g) 250 300 350 400 450 500 0 50 100 150 200 250 R 2 =0.7758 p<0.001 CI Echo (ml/min/g) CI Catheter (ml/min/g) B C Cardiac imaging. (A) B-mode image of a mouse heart with M-mode analysis of the left ventricle. (B) Echo-derived cardiac index and (C) correlation between echo and catheter derived methods of calculating Cardiac index. B A Tumour progression and vascularisation. (A) B-mode image of a mouse tumour with measurements. (B) B-mode image of a mouse tumour with measurements and blood flow with VEGF labelled microbubbles. Hind Limb Ischaemia. (A) Doppler flow images of ischaemic mouse hind limbs. (B) Quantification of serial % Flux over 35 days. A B Cardiac MR. Anatomical axial view of a mouse heart showing left and right ventricle. A B Contact: Allan Lawrie Data provided by Allan Lawrie, Abdul Hameed & Munitta Muthana
Transcript of Small Animal Imaging Poster - University of Sheffield · Doppler Flow Imaging Magnetic Resonance...
Ultrasound Imaging
Small Animal Imaging Facilities within University Departments - Towards a Core Facility
Presented by FRIC Small Animal Imaging Working Group
Doppler Flow Imaging Magnetic Resonance Imaging
Bioluminescence/fluorescence Imaging
Moor Instruments LD12-HR• Non-invasive laser doppler• Angiogenesis• Hind limb ischaemia• Spreading Cortical Depression
Doppler Flow ImagesControl P2Y12-/-
Caliper Life Sciences IVIS Lumina II• Non-invasive bioluminescence• Non-invasive fluorescence• Cell trafficking• Gene therapy/expression• Tumor development & regression• Bioluminescent transgenic lines
Intravital MicroscopyOptical imaging of • Bioluminescence• Fluorescence• Nanoparticle• Cell tracking/adhesion• Blood velocity• Permeability
Intravital imaging. (A) P22 rat sarcoma growing in a dorsal skin flap window chamber. Image was obtained using multiphoton fluorecence microscopy following i.v. administration of 70 kDa FITC-dextran. High spatial resolution is obtained and repeat imaging is possible. (B) Fluorescently labelled leukocytes can be used to assess rolling, adhesions and transmigration in post-capillary venules for the mouse cremaster muscle.
Bruker 7 Tesla, 310mm Bore MRI with Oxford Instruments HyperSense DNP Polariser.• Non-invasive anatomical scans• Cardiac scans• Tumour development• Perfusion, oxygenation and metabolism• Neurological imaging
Tumour metabolism. (A) 1H anatomical image of rat tumour. ( B ) T u m o u r u p t a k e o f hyperpolarised 13C-labelled pyruvate. (C) Tumour signals for pyruvate and lactate over time, which can be ana lysed to calculate rate constants.
Contact: Claire Lewis & Gill TozerData provided by David Dockrell & Helen Marriott
Contact: Sheila FrancisData provided by Sheila Francis, Tim Chico & Rob Storey
Contact: Jason Berwick, Anneurin Kennerley, Martyn Paley & Gill Tozer
Data provided by Steve Reynolds, Samira Kazan, Anneurin Kennerley & Allan Lawrie
Contact: Gill Tozer, Nicky Brown & Vicky RidgerData provided by Gill Tozer & Vicky Ridger
P22 tumour
phantom
Trafficking of S. pneumoniae. (A ) Mice instilled with bioluminescent S. pneumoniae. Images show persistence of bacteria of the WT mice and shows how
changes in bacterial burden can be followed in real time. (B) higher bacterial numbers in the blood (CFU/ml).
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A B
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B C
VisualSonics Vevo 770• Non-invasive rodent imaging• Qualitative & Quantitative• Cardiac / vascular imaging• Echocardiogaphy• Tumor detection• Tumor quantification• Blood flow
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d21 after MCT
Anti-OPG
d21 after MCT
Baseline IgG !-TRAIL !-OPG
*LV CI(!l/min/g)
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250 R2=0.7758p<0.001
CI Echo(ml/min/g)
CI Catheter(ml/min/g)
B C
Cardiac imaging. (A) B-mode image of a mouse heart with M-mode analysis of the left ventricle. (B) Echo-derived cardiac index and (C) correlation between echo and catheter derived methods of calculating Cardiac index.
BA
Tumour progression and vascularisation. (A) B-mode image of a mouse tumour with measurements. (B) B-mode image of a mouse tumour with measurements and blood flow with VEGF labelled microbubbles.
Hind Limb Ischaemia. (A) Doppler flow images of ischaemic mouse hind limbs. (B) Quantification of serial % Flux over 35 days.
A B
Cardiac MR. Anatomical axial view of a mouse heart showing left and right ventricle.
A B
Contact: Allan LawrieData provided by Allan Lawrie, Abdul Hameed & Munitta
Muthana
