Reaction Analytics Inc. ©2011 Slide 1

“iFlow”Flow Analytics System

Reaction Analytics Inc.

Makers of the iChemExplorer

Reaction Analytics Inc. ©2011 Slide 2

Flow Chemistry Opens Doors

• Scale-up microwave chemistry– Similar temp (250 C) under pressure

• Manage hazardous reactions– Dilute products in “closed” volume

• Perhaps a path to “new” chemistry– “Click Chemistry”– Reaction under Critical Fluid conditions

Reaction Analytics Inc. ©2011 Slide 3

Flow Chemistry Pitfalls

• Heterogeneous Chemistry

• Precipitates

• Addition and removal of catalysts

• Dilute reaction concentration

• Variations in Reactor Design to accommodate chemistry requirements

• And more you can think of…

Reaction Analytics Inc. ©2011 Slide 4

How Flow Can WorkDiscrete – Reaction Segments

• Reaction Solvent as Mobile Phase• Components mixed on sample tray• Mixture injected to Solvent Flow• Fluorescent Spacer between segments

Continuous – reagents flow with the solvent directly through the reactor

SpacerRxn

SegmentSpacer

RxnSegment

Reaction Analytics Inc. ©2011 Slide 5

Flow Chemistry What If’s• …Workflow could support process

screening AND continuous production

• …Samples with analytical results are available for every reaction

• …Hardware and software are robust and serviceable

• …the Flow Reactor is easily optimized to the chemistry

Reaction Analytics Inc. ©2011 Slide 6

iChem for Batch

• iChemExplorer uses vials as reactors and the HPLC to automate sample prep, collection and analysis

Reaction Analytics Inc. ©2011 Slide 7

FlowChem System Diagram

Reactor SectionWith fittings

To SampleCollection

To Analysis By LC/MS

HPLC AutosamplerWith ICEbox to Prepare ReactionSegments

ReactionSolvent

HPLCPump

Reaction Analytics Inc. ©2011 Slide 8

FlowChem System

ReactionSolvent Pump

Autosampler(1367)

ColumnCompartment

Detector

AnalyticalSolvent Pump

ColumnCompartment

Detector

Mass Spec

FractionCollector

X

FlowReactor

All Boxes in Bone areAgilent HPLC modules

Reaction Side Analytical Side

iChemExplorer

Reaction Analytics Inc. ©2011 Slide 9

Use of Agilent HPLC Modules• Control iFlow system with methods and

sequences in Agilent Chemstation v.03.01 or higher

• Mix and match modules BUT compatible firmware is required in all modules on CAN

• See Agilent Service for parts and modifications

Reaction Analytics Inc. ©2011 Slide 10

Note on Flow Reactor

• Keep it Simple– Tube with HPLC fittings

wound on a spindle– Spindle with tube on a

temperature-controlled Hotplate

iFlow can communicate with the IKA Hotplate to control temperature and record with analytical results

Example of Accendo Reactor

Reaction Analytics Inc. ©2011 Slide 11

iFlow Advantages

• iFlow automates prep of reaction segments

• HPLC pump delivers 600 bar (9000 psig) to clear obstructions

• iFlow publishes analytical results for immediate viewing and to Microsoft Excel for presentation and storage to e-lab notebook

Reaction Analytics Inc. ©2011 Slide 12

Thanks for your attention

Makers of the iChemExplorer, Reaction Analytics Inc. now offers the iFlow for optimization of flow chemistry

• To learn more please contact us at…

administrator@reactionanalytics.com