Skin & Bio Research ;@D

Skin & Bio Research

Scientific Beauty SubstantiationGlobal leader in Asian cosmetics testing

Ellead is a clinical and biotechnology company which is specialized in cosmetics and food supplement. Since 2001, Ellead has been performing clinical studies according to protocols for efficacy, safety, sensory tests of cosmetics, cosmeceuticals, and functional food products with great accuracy and reliability.

• Good Laboratory Pratice (GLP) certified by Korea Ministry of Food and Drug Safety

• ISO 9001: 2008 certification

• IRB (Institutional Review Board) registration

2016

2015

2014

2013

2012

2011

2007

2006

2005

2004

2003

2001

- Certified Good Laboratory Practice (GLP) by MFDS - Received Best Work Environment Award

- Establishment of in vitro research center at Osong, Korea - Development of improved evaluation method of UVA sunscreen (MFDS Cosmetic Efficacy Evaluation Guideline)- Validation of in vitro UVA evaluation method (MFDS)

- Presentation on Functional Cosmetics in Korea at One Asia Beauty Forum in China 2014- 2014 Venture Industry Activation Merit Award (received MOTIE Minister Award)- Developed evaluation method for irritation test using artificial skin- Development of evaluation method for edema improvement and skin blood flow (MFDS Cosmetic Efficacy Evaluation Guideline)

- Registered IRB with Korea Centers for Disease Control and Prevention - Development of evaluation method for dark circle alleviation (KFDA Cosmetic Efficacy Evaluation Guideline)

- Development of evaluation method for proper acne skin care (KFDA Cosmetic Efficacy Evaluation Guideline)

- Changed CI and opened second R&D Center- Registered IRB with US Food and Drug Administration - Received Million Dollar Export Award

- Field trip of cosmetic officialdom of China, Thailand, Philippines and Indonesia

- Certified as technically innovative (INNO-BIZ) company- Development of evaluation method for water-resistant sunscreen of SPF (KFDA Cosmetic Efficacy Evaluation Guideline) - Certified as venture company

- Acquired ISO 9001:2000 - Technology exchange with “DermExpert”

- Certified as the industry’s first R&D center by Korean Industrial Technology Promotion Association

- Established “Ellead”

History

Ellead | Skinn & Bio Research

Core Values

Sophistication

Trust

Our dermatologists have various clinical experiences and our skilled researchers provide the most suitable testing method to meet the demands of our customers. We perform clinical trials in accordance with Good Clinical Practice (GCP) under the supervision of our dermatologists.

AdvanceIn order to correspond with the current rapidly changing market, Ellead strives to extend its business spectrum to provide total clinical evaluation service focused on customer satisfaction by building a network with research and development centers and universities. We continue to develop new instru-ments, software and studies to maintain our advanced technologies.

RelianceEllead has been acknowledged for producing reliable and accurate results and accredited by the government. Many research results were presented at academic conferences. Now multinational corporations trust our research results and maintain a business partner.

• Standard Operating Procedures (SOP) set to meet various international regulations

• Studies conducted based on GCP guideline

• Ellead Institutional Review Board (IRB) registered with Korea and US FDA

• Quality Assurance system for high reproducibility and accuracy

• State of the art GLP compliant facilities

• Dermatologist-controlled studies

• Patented scientific photography system

• Multiple instruments to ensure reliability

• Various protocols tailored to each customer’s needs

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Quality Management

Efficacy / Safety / Sensory Evaluation

Testing Overview

Testing for

- Cosmetic products

- Active ingredients

- Raw materials

- Hair care products

- Toiletries

- Household products

- Functional foods

- Cosmetic devices

Ellead | Skin & Bio Research

Clinical test forEfficacy evaluation

Clinical test forSafety evaluation

- Functional cosmetics- Functional foods- Quasi-drugs

Service fields

test for cosmeticingredients and products

- Cytotoxicity test- Efficacy evaluation

- Primary patch test- Repeated insult patch test- Cumulative irritation test

Clinical test

In v

itro

effic

acy t

est

Safety test

In vitro

CLINICAL EVALUATION

Clinical safety test

Patch testSafety testing of cosmetic ingredients and finished products is of utmost importance. A variety of patch test designs are available to test for irritation or sensitization in the product development process.

Primary patch test

Repeated insult patch test (RIPT)

Cumulative irritation test (CIT)

Comedogenicity testComedogenic potential is tested by counting the number of comedones.

Follicular biopsy test on the back

Home use test with visual assessment

In-use testIn-use test is designed to test your product on the consumer’s view and dermatologist examination.

Self-assessment questionnaires (satisfaction & tolerance)

Adverse reaction

Eye irritation testEye irritation test is conducted in collaboration with ophthalmologist to study the level of sting and irritation in the eye that occur from contacting a product.


In Korea, anti-wrinkle, whitening and sunscreen products are categorized as functional cosmetics

Anti-aging

Anti-wrinkle*

Skin whitening*

Sunscreen (SPF, PA, Waterproof, Sweatproof)*

Skin hydration & lasting effect

Trans-epidermal water loss (TEWL)

Skin barrier recovery

Skin elasticity · Dermal density

Facial lifting

Skin sebum · Blackhead

Cleansing (Pore, Make-up)

Skin desquamation · Turn over

Skin gloss · Translucency

Dark circle

Cellulite · Puffiness · Stretch mark

Skin temperature

Microcirculation

Anti-microbial (Handwash)


Clinical efficacy test

Skin careClinical efficacy tests are conducted to test and substantiate the product’s intended purposes. We offer a wide range of tailored studies to claim your product’s efficacy and to maximise its impact in the marketplace using the latest equipment and technology.

Various test areas

Anti-wrinkle

Whitening

Before After

Available standards:

CLINICAL EVALUATION


SPF PA Water resistance In vitro

MFDS

ISO 24444

FDA final rule (2011)

Cosmetics Europe

CFDA

MFDS

ISO 24442

JCIA

CFDA

MFDS water resistance


Colipa water resistance

CFDA

ISO 24443


SunscreenWe have many Multiport Solar Simulators dedicated to each SPF, PA and water resistance test to offer multiple product testing according to the desired standard listed below.

We also offer in vitro SPF and PA test according to ISO 24443 and US FDA standards.


Skin temperature

Facial lifting Dermal density

Cheek sagging

Before After

Color durability and homogeneity

Hydration of lips

Eyelash curling



Make-upA variety of makeup products from foundations to eye makeup products can be tested to prove their efficacies on improving skin tone and lightening as well as for long lasting coverage, waterproof, curling and volumizing effect.

Hydration

Desquamation

Roughness

Lip careLip care products are applied topically to help moisturize and relieve skin dryness and chapping, and are tested for such claims.

Makeup color lasting effect · Homogeneity

Makeup coverage & lasting effect

Mascara & eyeliner waterproof effect

Eyelash curling & volume

Nail color lasting effect

Before After Overlay

Before After

CLINICAL EVALUATION



Hair gloss

Hair damage care Scalp desquamation

Hair growth Hair volume

Hair careA wide range of hair product testing is available including hair growth studies with phototrichogram as well as hair condition, color, strength and style testing services.

Hair growth

Hair damage

Gloss · Hydration

Tensile strength · Elasticity

Curling · Volume

Dye color durability

Scalp sebum · Desquamation

Scalp hydration · Elasticity

Before After

Before After Before After Before After

Intimate hygieneIntimate hygiene products are tested in collaboration with gynecologist and dermatologist.

Vaginal health grading

pH measurement

Skin hydration

Self-assessment



Oral careOral care products, such as tooth paste or mouthwash, can be tested for tooth whitening effect, oral hygiene and breath odor.

Tooth whitening

Oral hygiene

Breath odor

Acne study

Quasi-drugsQuasi-drugs are the products that have minimal to moderate pharmacologic (cosmeceutical) activity. The efficacy tests for quasi-drugs are conducted according to available guidelines.

Hair loss

Acne

Atopic dermatitis

Anti-microbial (Sanitizer)

Deodorant

Dandruff

Before After

CLINICAL EVALUATION

Functional foods


Visual assessment

Skin hydration · Wrinkle · Elasticity

Blood examination · Urine examination

Skin careEfficacy claims such as anti-wrinkle, whitening and hydration effects and safety of food supplements are tested after the approval of Ellead Institutional Review Board.

GLP study (certified by MFDS)

Skin irritation test Ellead has acquired Good Laboratory Practice (GLP) certification by Korea Ministry of Food and Drug Safety (MFDS) for in vitro Skin Irritation: Reconstructed Human Epidermis Test Method (OECD TG439).

in vitro


In vitro test

In vitro test

MTT assay

WST-1 assay

Alamar blue assay

Cytotoxicity & cell viability Nitric oxide inhibition assay

Cytokine inhibition assay (IL-6, TNF-α)

Protein inhibition assay (COX-2, PGE2)

Anti-inflammation

DPPH radical scavenging assay

ORAC (Oxygen radical absorbance capacity) assay

Total polyphenol quantification assay

ROS (Reactive oxygen species) scavenging assay

Anti-oxidant mRNA expression analysis

(Aquaporin, Hyaluronan synthase, Involucrin, Filaggrin)

Protein expression analysis (Involucrin, Filaggrin)

Moisture & Skin barrier

Collagen synthesis assay

Collagenase inhibition assay (MMP-1, MMP-3)

Anti-wrinkle Skin Irritation

: Reconstructed Human Epidermis Test

Skin absorption test using diffusion cell

3T3 NRU phototoxicity test

Alternatives to animal testing

Elastase inhibition assay

Elasticity

mRNA expression analysis by RT-PCR, RT-qPCR

Protein expression analysis by Western blot

Others tyrosinase inhibition assay

DOPA oxidation inhibition assay

Melanogenesis inhibition assay

Whitening

testing is essential in raw material / active ingredient development. Ellead offers various efficacy and safety tests customized to your needs at our new Ellead Osong research center dedicated solely for testing.

in vitro

in vitro

in vitro

in vitro

in vitro

in vitro

CLINICAL EVALUATION

Ellead Co., Ltd. www.ellead.com[HQ] 325, Hwangsaeul-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea 13590Tel: +82-31-709-9030 | Fax: +82-31-703-9071 | E-mail: [email protected]

[BRANCH] Venture center II, 194-41, Osongsaengmyeong 1-ro, Osong-eup, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do, Republic of Korea 28160 Tel: +82-43-715-5371 | Fax: +82-43-715-5377 | E-mail: [email protected]

Sophistication Advance Reliance

• ISO 9001:2008 Certified• GLP (Good Laboratory Practice) Certified• IRB (Institutional Review Board) Registered

Skin & Bio Research ;@D

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