Single-Use customised fermenter - 英宏德企業有限公司 · Single-Use customised fermenter...
Transcript of Single-Use customised fermenter - 英宏德企業有限公司 · Single-Use customised fermenter...
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Single-Usecustomised fermenter
July 2014
Scalable platform for microbial fermentation
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World first configurable Single-Use-Fermenter
• BactoVesselisavailableinOD110mmsizeforbottomdriveonMagnetic-Stirrer-Table(MST)orintheBio-block
• BactoVesselisavailableinOD130,OD150,OD200withSingle-Use-JacketforHead-Plate-Drive(HPD)orMagnetic-Stirrer-Table(MST)accept-ingmostservomotors
• BactoVesselavailableinOD110,OD130,OD150,OD200,OD250comeswithHead-Plate-Drive(HPD)accept-ingmostservomotors
• BactoVesselissoonavailableinseri-oussize,OD400withStrong-Head-Plate-Drive(SHPD)Configuration
Choose your own design =
fully configurable SUF
Scalable platform =
choose from 2 to >75 litre Vessel Volume
Outstanding performance =
>1,500 rpm capacity
Manufactured entirely from
mono-component rigid plastics
Much more information available on www.cercell.com
Shownafewofthe2,000differentcomponentsfromtheConfigurator Tool abletosatisfyanyrequest
What do I get?
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BactoVessel
Basic specifications for BactoVessel are:• PolycarbonateandNyloncomponents(nomulti-componentplasticfoil!)
• NumberofstandardPG13.5portsaccordingtotheSUFdiameter
• 6differentdiametersand4differentheight
• Turbine(s)forbi-directionalrotation/up-flowcombinedaxial/radialfluidcirculation
• TheSUFbearingsdesigned>1,500rpmforoneweekfermentationprocess
• Baffledvesselforcombinedaxialvortexmixinganddonutshapeflowpattern
•DesignedforNewtonianfluidswithmaxviscosityof50,000cP(similartoyourpreferredstiff,coldgoodmorningyogurt!)
• Designedfor50mBaroperatingpressure
Specification
Much more information available on www.cercell.com
Customised5.7litreSUFforHead-Plate-DrivenexttotheRe-Usable-Jacketforcoolingpriortheinstallation.
Fully configurable BactoVessel´s are created by selecting various optional components via the Configurator Tool:• Severalvesselheating/coolingoptionssuchasintegratedorre-usableheatexchanger
• Variousaerationmethods;microporesparger,microporeL-spargers,ringholespargers,L-spargers,headspacegasexchange
• SeveraldifferentfluidIn&Outmethodsandexhaustmethodstoselectfrom
•Arangeofsensors,Single-Use-Sensor(SUS),liquidlevel/foamsensors
•Optionallyoperatingpressureupto500mBarforOD110,OD130andOD150
•Optionallyoperatingpressureupto500mBarforOD200-OD250requiresacovercageincombinationwithRUJ
• DeliveredhighprecisionE-beam(32kGy)irradiatedindualpolyesterfoilbags
• AllcomponentsinmediacontactareISO10993,FDAapprovedorUSPclassVIcertifiedmaterials.
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BactoVessel facilitate a range of different radial mixing turbine principles: Rushton, Jacob, Smith, Hjort and Bakker:
•OuterDiameter,ODmm:40,50,60,70,80,90,100and120
•Numberofbladesare6or8dependingonprinciple
• Bladeshapes:flat,symmetrichalfcircle,symmetricconcav,asymmetricconcav
• Jacob´sflatbladeangledin75degreefromvertical
Heightofbladesisfixedaccordingtotheturbinediameter.DimensionofturbineandbladedesignedselectablefromtheConfigurator Tool.IfoneormoreaxialmixingMarineimpellersisrequiredintheBactoVesselsuchisavailableinaselectablediameter,3blade,16degreebladeangleddesign.
Two identical BactoVessel OD150 mmintegrating3baffles,ringspargerandRushtonandSmithturbines.Thetwoturbinesperformsverydifferent!
Agitation in BactoVessel Turbines
Unlimited range of turbines
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BafflesBaffles are obstructing vertical arranged vanes or elongated plates inside the vessel needed to stop the radial swirl inside the fermenter and convert the rotational flow to axial mixing.Withoutbaffles,thetangen-tialvelocitiescomingfromanyturbine(s)causestheentirefluidmasstospincreatingacentralvortex.Baffles,sotospeak,increasethefrictiontothevesselinnerwallsurface.BactoVesselbafflesarestraightflatplatesverticallyandperpendicularorientedclosetotheinnersideofthevesselattachedtoandextendingfromthebottomuptoaboveliquidsurface.
NumberofbafflesinBactoVesselrangefrom3to6dependingonthefermenterdiameter.Thebafflewidth(B1)istypicallyT/10-T/12ofthevesselinnerdiameter(T1).BafflesarelocatedwithdistanceofT/72–T/50fromthewall.
OD130 x 420 mm BactoVessel customisedtoend-userrequirementintegrating3baffles,ringsparger,dualIn&Out,HPDdriveandtwospecificRushtonturbines.
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Since oxygen is sparingly soluble in water, it’s one of several growth-limiting factor in fermentations. Thoughdealingwithaerobicmicro-bialapplicationsithelpsthatthecells,bacteria,yeast,etcarealmostinde-structiblefacilitatinghighRPM,suchasupto>1,500RPMwithBactoVessel.
Aeration in BactoVesselThe Rushton 100 % radial flow pattern turbine are a most cost effective design with performance generally limited by pockets or cavities, which cling to the back of the blade at high gas flow rates and/or RPM.Rushtoncausecavitation,considerablestreamliningandpowerdrawreduction.Whengasflowgetstoohigh,thepocketscanbridgebetweenblades,resultinginfloodingandseveremechanicalinstability.RushtonRT8hasthehighestun-gassedPowerNumberofanycommonturbine.
Smithturbinesisahighefficiencyagitatorforveryhighgasdispersionapplications.Charac-terizedbysymmetricalcurvedpipesectionsmountedtoarounddiscresultinginlowPowerNumberandincreasedefficiency.ThisparticularSmithmodelST6hashalftheun-gassedPowerNumbercomparedtotheRushtonturbine.
Ring sparger
Two photosshowaOD150x320mmBactoVesselequippedwiththeadvancedOD60Smith,6bladesturbinedesignplusMarineimpeller,3baffles,1nl/minWVgasinjection.
• At 0 RPM under static condition
• At 1000 RPM. Its worth to notice the limited foam generation even at such high RPM. Head space is in general free of foam
0 RPM
1000 RPM
BactoVessel facilitate aeration / gas injection throughaseriesof0.5mmholesarrangedinacirclebelowtheprimaryturbineintegratedasapartofthevesselbottom.NumberofholesdependingontheturbinediameterorchoicesthroughtheConfigurator Tool.
Much more information available on www.cercell.com
Top class kLa values
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This Smith symmetrically arranged curved (half pipe) blade design from the 1980s is able to handle increased
injected gas volume. CandispersetwicethegascomparedtotheRushtonbeforeflooding.Doesnotexperienceasgreatapowerdrop-offduetogas
loadingcomparedtotheRushtonturbine.Recommendedcombinedwithanup
pumpingMarineimpeller.
Revolutions
ExhaustSingle-Use-Gas-Cooler are available in three sizes and combines inner boro silicate glass tube for excellent heat transfer and outer PC tube and cooling liquid connections. Alltrans-parenttubesallowtheusertoinspectthefunctionality.
Large size –indesignphase.Max100nl/min/37°gasvolumecapacity.
ConfigurationviatheConfigurator Tool
Small size–ID/OD8/12x270mmglasstube,lowerendinletandoppositeoutletendfittedwithID/OD10/14mmsiliconehose,outletholdingoneormore26cm2surfaceareasterilefilter.Coolingarea68cm2suitableformax2nl/min/37°gasvolumecapacity.
Medium size–ID/OD24/30x300mmglasstube,lowerendinletID/OD18/22mmsiliconehose,andoutletfittedwithID10/14mmsiliconehosepieceand400cm2surfaceareafilter.GlasstubefilledwithRaschig6x6mmglasstubeelements.Coolingarea500cm2suitableformax20nl/min/37°gasvolumecapacity.Coolingliquidin/outletbeingG1/8”forID6mmhoseconnection.
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Sensors and sampling
Sampling
Even the shown photo is a CellVessel bio-reactor the In&Out system for BactoVessel share the same principles and specifications. ViatheConfigurator Tool youcanchooseanycombinationofhoses,connectorsforfluidspassingIn&OutoftheBactoVesselheadplate.AsIn&OutrangeofaccessoriesisbasedonthePG13.5pluganywishispossibletofullfil
Unlimited possibilities in connections
The CellVessel bioreactor use
thesameIn&Outsystem
specificationastheBactoVessel.
Much more information available on
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Single-Use-SensorsBenefits:
• IntegratedSUSreducecontaminationrisk
• Saveshoursofpreptimeandlabor,asnoautoclavingorsterilisationisneeded
• EnablesSUSsetuprightonthebenchtop–nobiosafetycabinet/hoodneededforoperation
•Noautoclavingfacilityisneeded
• ChooseSUSviaConfigurator Tool
Single-Use-Sensors (SUS) offer many advantages
Express
installation
Much more information available on www.cercell.com
Bio mass sensors
• Capacitivesensingtechnologyallowspreciseon-linemonitoringofthecellmass,viablecelldensityaswellascellphysiologicalstate.
• Userscanalsotrackcellcyclechanges,modelapoptosis,andpredictproteintiterallinrealtime
DO Single-Use-Sensor
• Non-invasiveDOwellforVisiFerm™.Pre-installedwithSUScapintegratedtotransparentPCtube.
• ExtendsDOsensorlife,assensorisneverautoclaved
•DOsensordesignedwithdigitalsignalcommunicationthatfitsmostPCS
pH Single-Use-Sensors
• Pre-installed6monthdrystoragetolerantpHSUSsuchasOneFermorFermProbe
• ClassicaldimensionpHsensorwithextendedshelflifetimeofferingmonthsofoperation
• ClassicalpHsignalfitsanyPCS
•Availablecombinedwith°CanalougeoutputfordualdigitalsignalcommunicationviaARCmodule
ARC moule for SUS
• Pre-installedpHSUSOneFermVP6designedforconnectiontoARCanalogue/digitalmodule
• Availablecombinedwith°CanalougeoutputfordualdigitalsignalcommunicationviaARC
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BactoVessel in RUJWater conveying Process-Control-System (PCS) benefit from the RUJ heat exchanger for BactoVessel. TheRUJdesignaddsignificantsupportfacilitatingtheservomotormountedontheheadplate(HPD).RUJisfurtherrequiredforSUFoperatingpressureupto500mBar.
OD150x320 BactoVessel designedwithturbineandimpeller.SUFdesignedformicrobialapplicationwhereexothermalmicrobialreactionrequirescooling.
TheRUJmountedwitha5.7litreVVSUFwith2.5litreWVareabletoremoved~20watt/litremediapower.TheRUJallowfastturn-overtimeandhighestpossibleexchangeofenergy.ShownwithRectus21couplingsfordirectconnectiontoBioStatsetoforangehoses.
PreparationBactoVessel SUF weigthislessthan1/10oftheglass/steelSTRofcomparablevolume.AnyonecaneasilytumbletheSUFinasafeway.
The BactoVessel concept is to move weight from the STR, Stirred-Tank-Reactors made from glass / Stain-less Steel to “fixed” components. ThetraditionaljacketedStirred-Tank-Reactorshaswith5.7litreVVtypicallyaweightof18kilo.Notreallyeasytohandle!
TheBactoVesselSUFiskeptaslightaspossible.The5.7litreBactoVesselSUFweigthislessthan2kilo!Alltheweigthismovedtothefixedcomponent–RUJ!
Reduced
turn-around
time
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Heating & cooling with Re-Usable-JacketFor water conveying Process-Control-System (PCS) CerCell offer a stand-alone Re-Usable-Jacket (RUJ) accessory to insert the SUF. ThisjacketisanefficientheatexchangerandanadvancedoptionforSUFOD110–250mmandin225,320and420mmheight.AflexibleO-ringsealbetweentheremovableSUFtopflangeandsupportringallowsimplereplacementoftheSUF.SuchSUFareequippedwithhead-plateflangeasselectedintheConfigurator Tool.
Specifications for RUJ:
• TheRUJisoneofmanyaccessoriesfoundonwww.cercell.com/commerce
•Operatingtemperature5-60°C
•Maxjacketoperatingpressure400mBar–RUJequippedwithaspringloadedover-pressure,systemprotectionvalvesetat500mBar
• Recommendedwaterflow1litre/minperlitre/SUFWV
• TwoG3/8”femalethreadforIn/Outadapt-ers:OD10barborGL18maleglassthread(similartoUniVesselandothers)orRectus21couplings(similartoBiostatsetup)
•Weightranging5-10kilodependingonsize,theweightfacilitatesstablearrange-mentondesktop.
• Thetransparentrigidplastictubematerialgivesexcellentprocessview.
Water connections
ProudProduction ManagerwithBactoVesselinRUJ
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Fromlefttorigth:Rectus21toG3/8”coupletkit,G3/8”toOD10barbkit,G3/8”toGL18glassthreadkit,hoseadapterstoGL18kits
For water conveying Process-Control-System (PCS) theoptionalintegratedSingle-Use-Jacket(SUJ)heatexchangerisanefficientoptionfortheBactoVesselfermenterfamily.AllowtheuseofMSTupto13.3litreSUFsize.
Specifications:
• SelectedviatheConfigurator Tool
•Operatingtemperature5-60°Cheating/coolingwater
• Recommendedmaxoperatingpressureis500mBar
• Burstpressureat20°Cis<1Bar
• Recommendedcoolingflow1litre/min/WV
• StandardtwoG3/8”femalethreadcon-nectionsonjacket
• Adaptersavailableasaccessory:G3/8”toOD10barb,G3/8”toGL18maleglassthreadadapterlikeUniVessel,G3/8”toRectus21male/femalekit
• Thetransparentrigidselfsupportiveplas-ticmaterialgivesexcellentprocessview.
Heating / cooling with Single-Use-Jacket
16.4 litre VV CellVessel (OD250x320) viaConfigurator Tool equippedwithSUJ.ThetwofemalethreadedG3/8”connectionsarevisibleontherightsideoftheSUF.
Water connection
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BactoVesselequippedwithSIngle-Use-JacketandfemaleG3/8”threadedconnectionsforavarietyofadapters.
BactoVessel with SUJ
5.7 litre VV BactoVessel viaConfigurator ToolequippedwithSUJ
1. FHC–Flexible-Heating-CoolingelementwrappedaroundtheSUFwith5.5litreVV,2.5litreWV,removedpower~5watt/litremedia
2. AluminiumRHScoilencapsulat-ingtheSUFwith5.5litreVV,2.5litreWV,removedpower~10watt/litremedia
3. IntegratedSingle-Use-JacketonSUFwith5.5litreVV,2.5litreWV,removedpower~20watt/litremedia
4. IntegratedjacketonUniVesselSTR-glass/steel,removedpower~25watt/litremedia
Heat exchange
Cooling
as with
glass
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HPD drive• Head-Plate-Drive(HPD)forBactoVesselSUFoffercoupling/adapterkitstoservomotorsfromApplikon,Biostat,DasGip,BJ,NBS,Solaris,Finesse,BBI-biotechandotherPCSservomotors
• Re-usableservomotoradapterseasilyattachedtotheOD30mmHPDcentrebearingontheBactoVesselheadplate
• Forhighloadsamotorstandishelpful
BactoVessel 5.7 litrefacilitatingSmithturbineandMarineimpelleroperatingat1,500RPMmountedinRe-Usable-Jacket,RUJequippedwithservomotorsupportandSky-Supportforthegas-coolerandexhaustfilter.
Agitation methods
Kollmorgen AKM23DservomotordrivingviaHPDinBiostatsetupa5.7litreBactoVesselsubmersedinaRUJ
Use you
own PCS
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MST drive•Magnetic-Stirrer-Table(MST)Stain-lessSteelconstructionfeaturinglonglifeandsilentrunningtoothedrubberbeltonaluminiumsprock-ets,twolifetimesealeddouble-rowballbearings
•MSTrotor(undertheSUForSUB)isequippedwith12permanentmag-netsandabletotransfer>100wattpowerdependingontheparticularservomotorcapacity
• Dim:390x180x50mm,weightis5,7kilo
• ForBactoVesselwithSIngle-Use-Jacket,SUJ
MSTdrivinginthiscasea3.0litreCellVesselbioreactor.FullyindependentoftheparticularPCSservomotor.TheMSTadapter,seentotheleft,takescareofthisisssue.
Kollmorgen AKM23D servomotordrivingMSTinBiostatsetup Mostservomotorsfacilitatetheseadapters.
You get drive options
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SUF productsOur “Configurator Tool” can help you design a SUF to your User-Requirement-Specification.
BactoVessel sizes to select from
Vessel diameter, OD mm 110 130 150 200 250 400
Number of PG13.5 ports 7 7 9 10 10 12
Turbine diameter, OD mm 40-50 50-60 50-70 80-100 100-120 100-150
Vessel Volume, litre
225 mm 2.1 3.0 4.1
320 mm 4.2 5.7
420 mm 13.3 21.0
500 mm
600 mm 29.5 75.6
Specificationssubjecttochangewithoutnotice.CerCell,CellVessel,BactoVessel,Applikon,Biostat,DasGip,Finesse,Solaris,Hamilton,Visiferm,Presensareregistratedtrademarks.
CerCellApsMalmmosevej19CDK-2840HolteDenmarkwww.cercell.comphone+4545423393info@cercell.com
Thank you for your time
browsing this leaflet
You get our attention
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