Leeuwenhoek’s Microscope Leeuwenhoek’s Single-Lens Microscope .
Single Lens Imaging System
If you can't read please download the document
description
Single Lens Imaging System. Retina. Photoreceptors. Neurons. Cone. Rod. Optic nerve fibers. Retina. Fovea. Optic nerve. Figure 29.8B. Human photoreceptor cells are named for their shapes Rods Cones. ROD. Cell body. CONE. Synaptic knobs. Membranous discs containing visual pigments. - PowerPoint PPT Presentation
Transcript of Single Lens Imaging System
-
Single Lens Imaging System
-
Figure 29.8BRetinaOptic nerveFoveaOptic nerve fibersRetinaPhotoreceptorsNeuronsConeRod
-
Human photoreceptor cells are named for their shapesRodsConesFigure 29.8ACell bodySynaptic knobsMembranous discs containing visual pigmentsRODCONE
-
Slide 15Slide 15Slide 15Vertebrate EyeRods
Cones
-
Three Cone Opsins (red, green, blue)
-
R(ed), G(reen) B(lue) Monitors
-
Robert Hooke (1635 - 1703)Built one of the first useful compound microscopesObserved structure of corkCoined the term Cell.Published Micrographia (1665)
-
1665 Hooke publishes Micrographia
1678 van Leeuwenhoek observes protozoa (little animals)
1838-9 Schleiden & Schwann proposed Cell Theory
1860 Pasteur confirms Cell Theory
1931 Ruska invents electron microscope
1932 Zerniki develops phase contrast microscopy
1955 Minsky invents the laser scanning microscope (LSM)
1989 Webb, Denk & Strickler invent multiphoton LSM
-
Anatomy of a Compound Microscopebaselamp housingsubstage condenserstagestandtrinocular tubeeye pieceobjective turret
-
Upright Microscope
-
Contrast MicroscopyKoehler IlluminationDark FieldPhase ContrastDifferential Interference ContrastHoffman Modulation
-
Condensers
-
Koehler Illumination
Condenser lens is adjusted so that an image of the field diaphragm is focused onto the object (specimen) plane
-
Koehler Illumination1. focus specimen 2. stop down field diaphragm until edges are visible 3. focus condenser until image of field diaphragm is sharp 4. open field diaphragm until edges are no longer visible
-
Conjugate Planes of Focus
-
Path of Imaging Rays Conjugate Planes
-
Path of Illuminating Rays Conjugate Planes
-
Light PathsIlluminating and imaging rays do not share conjugate planes
-
Projector vs Microscope
-
Microscope produces a virtual image of the back focal plane of the objective
-
HistochemistryHematoxylin & Eosin(H & E stain)Immunohistochemistry
-
Oil immersionn = 1.0n = 1.5n = 1.52n = 1.5
-
Effect of Oil
-
Contrast MicroscopyKoehler IlluminationDark FieldPhase ContrastDifferential Interference ContrastHoffman Modulation
-
Darkfield ImagesBrightfield Darkfield
![Deep Learning-Based Imaging using Single-Lens and Multi … · 2019. 10. 23. · conventional optics, we need a thicker lens for larger numerical aperture (or resolution) [1]. To](https://static.fdocuments.net/doc/165x107/5fe4e7d510a42d325b20a0eb/deep-learning-based-imaging-using-single-lens-and-multi-2019-10-23-conventional.jpg)
