Simons Institute for the Theory of Computing - Active Dendrites … · 2020. 1. 3. · Jim Heys...
Transcript of Simons Institute for the Theory of Computing - Active Dendrites … · 2020. 1. 3. · Jim Heys...
![Page 1: Simons Institute for the Theory of Computing - Active Dendrites … · 2020. 1. 3. · Jim Heys Mike Adoff Brad Randall Jason Climer Funding: NSF 1516235 NIH 1R01MH101297 ...](https://reader036.fdocuments.net/reader036/viewer/2022071416/61127a36ccc3a96f993adaaf/html5/thumbnails/1.jpg)
Active Dendrites Contribute to Hippocampal Place Field FormationMark Sheffield, Department of Neurobiology, The University of Chicago
![Page 2: Simons Institute for the Theory of Computing - Active Dendrites … · 2020. 1. 3. · Jim Heys Mike Adoff Brad Randall Jason Climer Funding: NSF 1516235 NIH 1R01MH101297 ...](https://reader036.fdocuments.net/reader036/viewer/2022071416/61127a36ccc3a96f993adaaf/html5/thumbnails/2.jpg)
2 m linear VR track CA1 somatic population imaging
Co-acquired CA1 somatic and basal dendritic imaging
![Page 3: Simons Institute for the Theory of Computing - Active Dendrites … · 2020. 1. 3. · Jim Heys Mike Adoff Brad Randall Jason Climer Funding: NSF 1516235 NIH 1R01MH101297 ...](https://reader036.fdocuments.net/reader036/viewer/2022071416/61127a36ccc3a96f993adaaf/html5/thumbnails/3.jpg)
Switching the Virtual Environment Causes Remapping of the Hippocampal Cognitive Map
Cel
l nu
mb
er
Cel
l nu
mb
er
1
7878
1
Track Position Track Position
Mean place field position Mean place field position
Linear track 1 Linear track 2
Virtual teleportation
78 Place cells covering linear track 1
![Page 4: Simons Institute for the Theory of Computing - Active Dendrites … · 2020. 1. 3. · Jim Heys Mike Adoff Brad Randall Jason Climer Funding: NSF 1516235 NIH 1R01MH101297 ...](https://reader036.fdocuments.net/reader036/viewer/2022071416/61127a36ccc3a96f993adaaf/html5/thumbnails/4.jpg)
Example cell: delayed place field
Lap
nu
mb
er
35
1
Track Position (cm)1 300
Delay
Example cell: Instant place field
The majority of place fields appear within the first ~15 laps of exposure to a novel environment
Sheffield et. al., Neuron, 2017
![Page 5: Simons Institute for the Theory of Computing - Active Dendrites … · 2020. 1. 3. · Jim Heys Mike Adoff Brad Randall Jason Climer Funding: NSF 1516235 NIH 1R01MH101297 ...](https://reader036.fdocuments.net/reader036/viewer/2022071416/61127a36ccc3a96f993adaaf/html5/thumbnails/5.jpg)
Soma & all branches Soma & no branches
Place field transient 1 Place field transient 2(same cell)
Branch spiking throughout the arbor can vary between place field traversals
![Page 6: Simons Institute for the Theory of Computing - Active Dendrites … · 2020. 1. 3. · Jim Heys Mike Adoff Brad Randall Jason Climer Funding: NSF 1516235 NIH 1R01MH101297 ...](https://reader036.fdocuments.net/reader036/viewer/2022071416/61127a36ccc3a96f993adaaf/html5/thumbnails/6.jpg)
Sheffield et. al., Neuron, 2017
Branch spike prevalence across basal dendrites is highest when place fields first appear
![Page 7: Simons Institute for the Theory of Computing - Active Dendrites … · 2020. 1. 3. · Jim Heys Mike Adoff Brad Randall Jason Climer Funding: NSF 1516235 NIH 1R01MH101297 ...](https://reader036.fdocuments.net/reader036/viewer/2022071416/61127a36ccc3a96f993adaaf/html5/thumbnails/7.jpg)
Branch spike prevalence predicts future place field location
Branch spiking
First few laps in novel environment
Somatic place field
High Low Low
Many laps in novelenvironment
Avg
bra
nch
sp
ike
pre
vale
nce
Sheffield et. al., Neuron, 2017
![Page 8: Simons Institute for the Theory of Computing - Active Dendrites … · 2020. 1. 3. · Jim Heys Mike Adoff Brad Randall Jason Climer Funding: NSF 1516235 NIH 1R01MH101297 ...](https://reader036.fdocuments.net/reader036/viewer/2022071416/61127a36ccc3a96f993adaaf/html5/thumbnails/8.jpg)
In-vivo spontaneous dSpike
Ex-vivo evoked dSpike
10 um
Localized dendritic branch spikes in CA1 basal dendrites
7 spines0.5 ms laser duration0.12 ms interstim interval<0.5 ms to stimulate them all
![Page 9: Simons Institute for the Theory of Computing - Active Dendrites … · 2020. 1. 3. · Jim Heys Mike Adoff Brad Randall Jason Climer Funding: NSF 1516235 NIH 1R01MH101297 ...](https://reader036.fdocuments.net/reader036/viewer/2022071416/61127a36ccc3a96f993adaaf/html5/thumbnails/9.jpg)
dSpikes occur prior to the formation of delayed place fields
Rest Spine transient Branch spike Dendritic spike
Matched image settings
10 um
Example cell: delayed place field
Lap
nu
mb
er
35
1
Track Position (cm)1 300
Sheffield et. al., Neuron, 2017
![Page 10: Simons Institute for the Theory of Computing - Active Dendrites … · 2020. 1. 3. · Jim Heys Mike Adoff Brad Randall Jason Climer Funding: NSF 1516235 NIH 1R01MH101297 ...](https://reader036.fdocuments.net/reader036/viewer/2022071416/61127a36ccc3a96f993adaaf/html5/thumbnails/10.jpg)
Ethan Goldberg & Douglas Coulter (2013)
Interneurons that target CA1 dendrites could regulate dendritic spikes
SOM+ Interneurons
Axons in CA1 basal dendritic layer
20 μm
Sheffield et. al., Neuron, 2017
![Page 11: Simons Institute for the Theory of Computing - Active Dendrites … · 2020. 1. 3. · Jim Heys Mike Adoff Brad Randall Jason Climer Funding: NSF 1516235 NIH 1R01MH101297 ...](https://reader036.fdocuments.net/reader036/viewer/2022071416/61127a36ccc3a96f993adaaf/html5/thumbnails/11.jpg)
Dendritic inhibition is transiently reduced during novel environment exposure
Sheffield et. al., Neuron, 2017
![Page 12: Simons Institute for the Theory of Computing - Active Dendrites … · 2020. 1. 3. · Jim Heys Mike Adoff Brad Randall Jason Climer Funding: NSF 1516235 NIH 1R01MH101297 ...](https://reader036.fdocuments.net/reader036/viewer/2022071416/61127a36ccc3a96f993adaaf/html5/thumbnails/12.jpg)
Localized dendritic spikes absent during place field Formation in NR1KO
Inducible knock out of NMDA receptors in CA1 neurons disrupts active dendritic signals
Branch spikes reduced during place field formation in NR1KO
WT mice
Sheffield et. al., Neuron, 2017
![Page 13: Simons Institute for the Theory of Computing - Active Dendrites … · 2020. 1. 3. · Jim Heys Mike Adoff Brad Randall Jason Climer Funding: NSF 1516235 NIH 1R01MH101297 ...](https://reader036.fdocuments.net/reader036/viewer/2022071416/61127a36ccc3a96f993adaaf/html5/thumbnails/13.jpg)
NMDA KO in CA1 neurons decreases the number of place fields that form
Sheffield et. al., Neuron, 2017
![Page 14: Simons Institute for the Theory of Computing - Active Dendrites … · 2020. 1. 3. · Jim Heys Mike Adoff Brad Randall Jason Climer Funding: NSF 1516235 NIH 1R01MH101297 ...](https://reader036.fdocuments.net/reader036/viewer/2022071416/61127a36ccc3a96f993adaaf/html5/thumbnails/14.jpg)
Sheffield et. al., Neuron, 2017
![Page 15: Simons Institute for the Theory of Computing - Active Dendrites … · 2020. 1. 3. · Jim Heys Mike Adoff Brad Randall Jason Climer Funding: NSF 1516235 NIH 1R01MH101297 ...](https://reader036.fdocuments.net/reader036/viewer/2022071416/61127a36ccc3a96f993adaaf/html5/thumbnails/15.jpg)
Acknowledgements
Dombeck Lab:
Mark Howe
Jim Heys
Mike Adoff
Brad Randall
Jason Climer
Funding:
NSF 1516235NIH 1R01MH101297
![Page 16: Simons Institute for the Theory of Computing - Active Dendrites … · 2020. 1. 3. · Jim Heys Mike Adoff Brad Randall Jason Climer Funding: NSF 1516235 NIH 1R01MH101297 ...](https://reader036.fdocuments.net/reader036/viewer/2022071416/61127a36ccc3a96f993adaaf/html5/thumbnails/16.jpg)
Acknowledgements
Sheffield Lab:
Can Dong (Grad student)
Chery Cherian (Tech)
Jo DiPietro (Post Doc)
Madeline Klinger (Tech)
Funding:
UChicago Start up
Whitehall Foundation
Sloan Fellowship