Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of...
Transcript of Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of...
![Page 1: Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of Experimental Ecology Oxana Habuštová Biology Centre,](https://reader036.fdocuments.net/reader036/viewer/2022062803/56649f215503460f94c396ea/html5/thumbnails/1.jpg)
Side effect of transgenic plantsSide effect of transgenic plants
Biology Centre, ASCR, v.v.i. Institute of Entomology
Department of Experimental Ecology
Oxana Habuštová
Biology Centre, ASCR, v.v.i. Institute of Entomology
Department of Experimental Ecology
Oxana Habuštová
![Page 2: Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of Experimental Ecology Oxana Habuštová Biology Centre,](https://reader036.fdocuments.net/reader036/viewer/2022062803/56649f215503460f94c396ea/html5/thumbnails/2.jpg)
Conducted researchConducted research
Monitoring of selected insect taxa and spiders on ten 0.5-ha plots in a 14-ha field in 2002-2005. Maize MON810 expressing Cry1Ab was compared with a parental, non-transgenic cultivar. Ten plants and five pitfall traps per plot were examined in 2-week intervals.
![Page 3: Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of Experimental Ecology Oxana Habuštová Biology Centre,](https://reader036.fdocuments.net/reader036/viewer/2022062803/56649f215503460f94c396ea/html5/thumbnails/3.jpg)
Field trialsField trials
![Page 4: Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of Experimental Ecology Oxana Habuštová Biology Centre,](https://reader036.fdocuments.net/reader036/viewer/2022062803/56649f215503460f94c396ea/html5/thumbnails/4.jpg)
Average temperatures
![Page 5: Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of Experimental Ecology Oxana Habuštová Biology Centre,](https://reader036.fdocuments.net/reader036/viewer/2022062803/56649f215503460f94c396ea/html5/thumbnails/5.jpg)
Precipitation profile
![Page 6: Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of Experimental Ecology Oxana Habuštová Biology Centre,](https://reader036.fdocuments.net/reader036/viewer/2022062803/56649f215503460f94c396ea/html5/thumbnails/6.jpg)
Quantification of Cry toxinQuantification of Cry toxin
![Page 7: Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of Experimental Ecology Oxana Habuštová Biology Centre,](https://reader036.fdocuments.net/reader036/viewer/2022062803/56649f215503460f94c396ea/html5/thumbnails/7.jpg)
Content of Cry1Ab/c (µg/g) in MON810 in 2003-2005 (no toxin was detected in the control maize)Content of Cry1Ab/c (µg/g) in MON810 in 2003-2005 (no toxin was detected in the control maize)
![Page 8: Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of Experimental Ecology Oxana Habuštová Biology Centre,](https://reader036.fdocuments.net/reader036/viewer/2022062803/56649f215503460f94c396ea/html5/thumbnails/8.jpg)
Numbers of dominating insects per ten plants in 2003-2005
![Page 9: Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of Experimental Ecology Oxana Habuštová Biology Centre,](https://reader036.fdocuments.net/reader036/viewer/2022062803/56649f215503460f94c396ea/html5/thumbnails/9.jpg)
Abundance of sucking insects in relation to Bt expression, date and year
![Page 10: Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of Experimental Ecology Oxana Habuštová Biology Centre,](https://reader036.fdocuments.net/reader036/viewer/2022062803/56649f215503460f94c396ea/html5/thumbnails/10.jpg)
Species
2003
2004
2005
Bt
Non Bt
Bt
Non Bt
Bt
Non Bt
Pterostichus melanarius (Illiger, 1798)
576
612
631
653
415
494
Calathus fuscipes (Goeze, 1777)
328
215
99
63
110
78
Poecilus cupreus (Linné, 1758)
375
451
4019
4593
2962
3456
Bembidion quadrimaculatum (L., 1761)
123
114
186
224
50
60
Trechus quadristriatus (Schrank, 1781)
17
23
27
24
14
18
Bembidion lampros (Herbst, 1784)
11
3
45
67
43
22
Pseudoophonus rufipes (De Geer, 1774)
154
178
94
68
168
273
Clivina fossor (Linné, 1758)
37
36
30
23
14
16
Dominant carabid species
![Page 11: Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of Experimental Ecology Oxana Habuštová Biology Centre,](https://reader036.fdocuments.net/reader036/viewer/2022062803/56649f215503460f94c396ea/html5/thumbnails/11.jpg)
Dominant spider species
![Page 12: Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of Experimental Ecology Oxana Habuštová Biology Centre,](https://reader036.fdocuments.net/reader036/viewer/2022062803/56649f215503460f94c396ea/html5/thumbnails/12.jpg)
Abundance of epigeic arthropods on Cry1Ab (Bt), data, year, and plot position
![Page 13: Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of Experimental Ecology Oxana Habuštová Biology Centre,](https://reader036.fdocuments.net/reader036/viewer/2022062803/56649f215503460f94c396ea/html5/thumbnails/13.jpg)
• Maize MON810 contains > 1 ppm Cry1Ab in the stem and folliage but undetectable amounts in the flowers and grain.
• It is not attacked by the European corn borer.
• None of the other herbivores living on the maize plants and none of their predators were affected.
• The compositions of epigeic beetles and spiders was not altered due to Cry presence.
Conclusions
![Page 14: Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of Experimental Ecology Oxana Habuštová Biology Centre,](https://reader036.fdocuments.net/reader036/viewer/2022062803/56649f215503460f94c396ea/html5/thumbnails/14.jpg)
Recent experimentRecent experiment
• Will performed in the corner of 60ha field• 14 ha will divided into 25 plots of 0.5ha each• MON 88017, isogenic, treated with insecticides,
reference cultivar A and B will be sowed• Weather condition• Precipitation profile • Plant dwelling insect • Soil insects by pitfall traps• Entomopathogenic nematodes• Yellow sticky traps with pheromone „Csalomon
PAL“ for Diabrotica virgifera • Yellow sticky traps for natural enemies• Content of Cry3Bb1 toxin
![Page 15: Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of Experimental Ecology Oxana Habuštová Biology Centre,](https://reader036.fdocuments.net/reader036/viewer/2022062803/56649f215503460f94c396ea/html5/thumbnails/15.jpg)
Design of experimental field in 2009-2011
![Page 16: Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of Experimental Ecology Oxana Habuštová Biology Centre,](https://reader036.fdocuments.net/reader036/viewer/2022062803/56649f215503460f94c396ea/html5/thumbnails/16.jpg)
Thank you for your attention!Thank you for your attention!