C190-E221

Shim-pack Scepter LC ColumnsExcellent stability & performance could be achieved under a wide range of LC conditions

Method Scouting Utilize excellent stability & performance under a wide range of LC conditions

Excellent stability and performance could be achieved under a wide range of LC conditions with Shim-pack Scepter LC columns, which are the next generation organic silica hybrid based columns. With different chemistry characteristics, Shim-pack Scepter columns are effective for method development/scouting with suitability for use in a wide variety of applications. With different particle sizes (1.9 µm, 3 µm, 5 µm) and different column dimensions, Shim-pack Scepter LC columns are fully scalable between UHPLC, HPLC and preparative LC making method transfer seamless between different laboratory instrumentation.

Shim-pack Scepter Chemistries

Shim-pack Scepter

Ligand Type

Particle

Particle Size

Pore Size

End Capping

pH Range

100% aqueous condition

USP Classification

Trifunctional C18 Trifunctional C18

Generic Purpose Type High Density Type

12 nm 8 nm

Proprietary

1 - 12

No

L1

No

L7

Yes

L1

1 - 10

Yes

L11

1 - 8

Yes

L43

2 - 10

N/A

L20

None

12 nm

Trifunctional C8Trifunctional Phenylbutyl

Trifunctional Pentafluorophenylpropyl

Trifunctional�Dihydroxypropyl

Organic Silica Hybrid

1.9 µm, 3 µm, 5 µm

Reveresed Phase

C8C18 HD-C18 Phenyl PFPP

HILIC

Diol-HILIC

pH2.9

pH6.1

pH9.9

C8C18 HD-C18 Phenyl PFPP

With excellent stability under a wide range of LC conditions, Shim-pack Scepter LC columns are effective for method scouting combining mobile phase pH and organic modifier.

Analytes

1. Saccharin (pKa = 2.2)

2. Dextromethorphan (pKa = 8.3)

3. Amitriptyline (pKa = 9.4)

4. N-Butyl paraben (pKa = 8.3)

5. Ibupurofen (pKa = 4.4)

Comparison of Chromatograms using Gradient condition with Acetonitrile

Outside of recommended pH

mAU

0

20

min0 2 4 6 8 1210

1

2 3 4 5

mAU

0

20

min0 2 4 6 8 1210

1

2 3 5 4

F140812A

mAU

0

20

min0 2 4 6 8 1210

1

2 3 4

5

mAU

0

20

min0 2 4 6 8 1210

2

3 4

5

1

mAU

0

20

min0 2 4 6 8 1210

4 5

1 ( peak 2, 3 : N.D.)

mAU

0

20

min0 2 4 6 8 1210

1

2 3,

4

5

mAU

0

20

min0 2 4 6 8 1210

1

2

3

5 4

mAU

0

20

min0 2 4 6 8 1210

1

2

3

4 5

mAU

0

20

min0 2 4 6 8 1210

1

2 3

4

5

mAU

0

20

min0 2 4 6 8 1210

1

2 3

4 5

mAU

0

20

min0 2 4 6 8 1210

1

2 3

4

5

mAU

0

20

min0 2 4 6 8 1210

1

2 3

4

5 mAU

0

20

min0 2 4 6 8 1210

1

2

3 4

5

mAU

0

20

min0 2 4 6 8 1210

1

2

3 4

5

Shim-pack Scepter Analytical Columns

Shim-pack Scepter Preparative Columns

Particle Size (µm)Chemistry C18 HD-C18 C8

Ordering Information

* Main P/Ns are described in the list. Please contact your local representative for guard columns and columns in dimensions other than described above.

ID (mm)� 2.1227-31012-03227-31012-04227-31012-05227-31012-06227-31014-03227-31014-04227-31014-05227-31014-06

227-31017-03227-31017-04227-31017-05227-31017-06

3227-31013-01227-31013-02227-31013-03227-31013-04227-31015-01227-31015-02227-31015-03227-31015-04

227-31018-01227-31018-02227-31018-03227-31018-04

4.6

227-31016-02227-31016-03227-31016-04227-31016-05227-31016-06227-31020-02227-31020-03227-31020-04227-31020-05227-31020-06

4.6

227-31030-02227-31030-03227-31030-04227-31030-05227-31030-06227-31024-02227-31024-03227-31024-04227-31024-05227-31024-06

2.1227-31026-03227-31026-04227-31026-05227-31026-06227-31028-03227-31028-04227-31028-05227-31028-06

227-31021-02227-31021-03227-31021-04227-31021-05

3227-31027-01227-31027-02227-31027-03227-31027-04227-31029-01227-31029-02227-31029-03227-31029-04

227-31022-01227-31022-02227-31022-03227-31022-04

4.6

227-31037-02227-31037-03227-31037-04227-31037-05227-31037-06227-31041-02227-31041-03227-31041-04227-31041-05227-31041-06

2.1227-31033-03227-31033-04227-31033-05227-31033-06227-31035-03227-31035-04227-31035-05227-31035-06

227-31038-03227-31038-04227-31038-05227-31038-06

3227-31034-01227-31034-02227-31034-03227-31034-04227-31036-01227-31036-02227-31036-03227-31036-04

227-31039-01227-31039-02227-31039-03227-31039-04

507510015050751001502505075100150250

1.9

3

5

Length (mm)

ID (mm)�Length (mm)Particle Size (µm)Chemistry Phenyl PFPP Diol-HILIC

2.1227-31063-03227-31063-04227-31063-05227-31063-06227-31065-03227-31065-04227-31065-05227-31065-06

227-31068-03227-31068-04227-31068-05227-31068-06

3227-31064-01227-31064-02227-31064-03227-31064-04227-31066-01227-31066-02227-31066-03227-31066-04

227-31069-01227-31069-02227-31069-03227-31069-04

4.6

227-31067-02227-31067-03227-31067-04227-31067-05227-31067-06227-31071-02227-31071-03227-31071-04227-31071-05227-31071-06

4.6

227-31057-02227-31057-03227-31057-04227-31057-05227-31057-06227-31061-02227-31061-03227-31061-04227-31061-05227-31061-06

2.1227-31053-03227-31053-04227-31053-05227-31053-06227-31055-03227-31055-04227-31055-05227-31055-06

227-31058-03227-31058-04227-31058-05227-31058-06

3227-31054-01227-31054-02227-31054-03227-31054-04227-31056-01227-31056-02227-31056-03227-31056-04

227-31059-01227-31059-02227-31059-03227-31059-04

4.6

227-31047-02227-31047-03227-31047-04227-31047-05227-31047-06227-31051-02227-31051-03227-31051-04227-31051-05227-31051-06

2.1227-31043-03227-31043-01227-31043-02

227-31045-03227-31045-04227-31045-05227-31045-06

227-31048-03227-31048-04227-31048-05227-31048-06

3227-31044-03227-31044-01227-31044-02

227-31046-01227-31046-02227-31046-03227-31046-04

227-31049-01227-31049-02227-31049-03227-31049-04

507510015050751001502505075100150250

1.9

3

5

Chemistry 10

227-31101-01227-31101-02

227-31104-01227-31104-02

227-31107-01227-31107-02

20227-31102-01

227-31102-02227-31102-03227-31102-04227-31105-01

227-31105-02227-31105-03227-31105-04227-31108-01

227-31108-02227-31108-03227-31108-04

30227-31103-01227-31103-02227-31103-03227-31103-04227-31103-05227-31106-01227-31106-02227-31106-03227-31106-04227-31106-05227-31109-01227-31109-02227-31109-03227-31109-04227-31109-05

10

227-31113-01227-31113-02

227-31110-01227-31110-02

Chemistry

Phenyl

PFPP

20227-31114-01

227-31114-02227-31114-03227-31114-04227-31111-01

227-31111-02227-31111-03227-31111-04

30227-31115-01227-31115-02227-31115-03227-31115-04227-31115-05227-31112-01227-31112-02227-31112-03227-31112-04227-31112-05

507510015025050751001502505075100150250

C18

HD-C18

C8

50751001502505075100150250

ID (mm)�Length (mm)ID (mm)�Length (mm)

© Shimadzu Corporation, 2018

www.shimadzu.com/an/

For Research Use Only. Not for use in diagnostic procedures. This publication may contain references to products that are not available in your country. Please contact us to check the availability of these products in your country.Company names, products/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation, its subsidiaries or its affiliates, whether or not they are used with trademark symbol “TM” or “®”.Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services, whether or not they are used with trademark symbol “TM” or “®”.Shimadzu disclaims any proprietary interest in trademarks and trade names other than its own.

The contents of this publication are provided to you “as is” without warranty of any kind, and are subject to change without notice. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication.

First Edition: September 2018, Printed in Japan 3655-09807-20AIK